Special Issues
Table of Content

Advances in Enhancing Grain Yield: From Molecular Mechanisms to Sustainable Agriculture

Submission Deadline: 31 October 2025 (closed) View: 1848 Submit to Journal

Guest Editors

Dr. Muhammad Umer Farooq

Email: umerpbguaf@gmail.com

Affiliation: Senior Scientist (Cereals), Wheat Research Institute, Ayub Agricultural Research Institute Faisalabad, 38850, Agriculture Department (Research Wing), Punjab, Pakistan

Homepage:

Research Interests: Biofortification, Phenotyping, Plant Physiology, Wheat Breeding, Crop Quality Control, Metallic trace elements, Selenium, Cadmium, Nutritional Physiology, Root Architecture System, Resource Management, Biochemical Assay, Wheat Breeding, Seed engineering, Environmental pollution, Sustainability

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Dr. Javed Ahmad

Email:javed1710@yahoo.com

Affiliation: Chief Scientist, Wheat Research Institute, Ayub Agricultural Research Institute Faisalabad, 38850, Agriculture Department (Research Wing), Punjab, Pakistan

Homepage:

Research Interests:Breeding, Genetic and Biotechnological research on wheat and Barley crops for variety development using conventional (Introduction, Selection, Hybridization) and non-conventional biotechnological techniques (Tissue culture, doubled haploid production using wheat x maize crossing system, molecular breeding).

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Dr. Sajid Fiaz

Email:sajidfiaz50@yahoo.com

Affiliation: Institute of Molecular Biology and Biotechnology, The University of Lahore, 54590 Lahore, Pakistan 

Homepage:

Research Interests:Genome Wide Association Analysis, QTL Mapping, Functional Genomics, Stress Breeding, Genetic Diversity Analysis, OMICs and Genotyping by Sequencing

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Dr. Muhammad Ahsan Asghar

Email:muhammad.ahsan@food.au.dk/ahsanasghar3853@gmail.com

Affiliation: Department of Food Science, Aarhus University, 8210, Aarhus N, Denmark 

Homepage:

Research Interests:Plant Stress Physiology and Biochemistry, Plant, Moleclar Biology, Sustainable Agriculture, Plant-Environment Interactions, Redox Changes under abiotic stresses, Light, Drought, Salt, Temperature, Metabolites, Hormones, Genes

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Summary

Improving grain yield is a critical challenge for ensuring food security and sustainable agricultural practices, especially in the face of pressing global challenges. The world’s population is projected to reach nearly 10 billion by 2050, increasing the demand for food while arable land is declining due to urbanization, land degradation, and climate change. Moreover, the food security is threatened by various abiotic stresses i.e. water scarcity, extreme temperatures, light intensity, salinity and nutrient deficiencies. Traditional agricultural methods alone are insufficient to meet these challenges; thus, this special issue focuses on multidisciplinary advances that span from molecular mechanisms to sustainable agricultural practices, aiming to explore how genetic improvement, crop physiology, and agronomic management can contribute to enhancing crop yields under changing climatic conditions. Recent advancements in molecular biology, particularly gene editing technologies like CRISPR/Cas9, provide powerful tools for precise genetic improvements. Understanding plant stress physiology and biochemical pathways is crucial for developing crops that can thrive in suboptimal conditions. Furthermore, the integration of digital technologies and precision agriculture can optimize resource use and enhance crop management strategies.


The significance of this research area is underscored by its vital contribution to global food security. As the world’s population continues to rise, innovative approaches are imperative for sustainably enhancing crop yields. The development of resilient crops capable of withstanding abiotic stresses is essential for adapting agricultural practices to fluctuating climate conditions, thereby securing stable food supplies in the face of environmental adversities. By integrating molecular techniques with sustainable agricultural methodologies, this field advocates for resource-efficient farming practices that mitigate environmental impacts. Furthermore, the convergence of diverse scientific disciplines encourages interdisciplinary collaboration, fostering innovation and expediting the formulation of novel strategies for crop improvement. Insights derived from this research can profoundly influence agricultural policies, directing investments and practices that bolster sustainable agriculture and food security. Additionally, improvements in crop yields are critical for the economic sustainability of farming, empowering farmers to thrive in an increasingly competitive market landscape. In essence, by deepening our understanding of molecular mechanisms and their agricultural applications, this research area plays a transformative role in shaping the future of global food systems and promoting sustainable agricultural practices.


The aim of this special issue is to bring together cutting-edge research and innovative approaches that enhance grain yield through a multidisciplinary lens, addressing the challenges posed by climate change, resource scarcity, and food security. This scope of this special issue includes topics but are not limited to:

-Applications of Molecular Biology and Gene Editing Technologies

-Advances in Plant Stress Physiology and Biochemistry

-Crop-Environment Interactions

-Advances in Plant Nutrition Signaling, Accessibility and Transformation

-Above and below ground research advancements

-Integration of Precision Agriculture and Digital Technologies Sustainable Agricultural Practices and Policy Recommendations.

-Molecular breeding; reverse genetic tools

-QTL Mapping for Yield and Quality related traits

-Genetic diversity analysis in cereals

-OMICs for crop improvement

-Application of plants genetics and biotechnology for stress breeding 


Keywords

plant physiologay and stress response, nutrition management and soil health, climate change, precision agriculture, molecular breeding, sustainability

Published Papers


  • Open Access

    ARTICLE

    Sunflower (Helianthus annuus L.) Hybrids: Strategic Crossbreeding Techniques to Efficiently Enhance Yield and Oil Quality

    Fida Hussain, Farooq Khan, Javed Ahmad, Heqiang Huo, Tao Jiang, Iqrar Rana, Sajida Habib, Muhammad Umer Farooq
    Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3231-3249, 2025, DOI:10.32604/phyton.2025.069654
    (This article belongs to the Special Issue: Advances in Enhancing Grain Yield: From Molecular Mechanisms to Sustainable Agriculture)
    Abstract The analysis of combining ability and heterosis is very important in enhancing the yield and oil quality of sunflowers under adverse conditions, and it reveals the potential of the parents and the mechanism of gene action. In this study, twenty-one hybrids were developed by crossing seven cytoplasmic male sterile (CMS) lines with three restorer lines and evaluated for agronomic and quality traits. Highly significant general combining ability (GCA) and specific combining ability (SCA) effects were observed, confirming the role of both additive and non-additive gene actions. Among the tested crosses, A-42 × R-86, A-92 ×… More >

  • Open Access

    ARTICLE

    Genomic and Functional Characterization of Thermophilic Paenibacillus sp. VCA1: A Biocontrol Agent Isolated from El Chichón Volcano Crater Lake

    Nancy Abril Martínez-López, Betsy Anaid Peña-Ocaña, Rodolfo García-Contreras, Toshinari Maeda, Reiner Rincón-Rosales, Federico Antonio Gutiérrez-Miceli, Víctor Manuel Ruíz-Valdiviezo
    Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2729-2743, 2025, DOI:10.32604/phyton.2025.068176
    (This article belongs to the Special Issue: Advances in Enhancing Grain Yield: From Molecular Mechanisms to Sustainable Agriculture)
    Abstract Species of the genus Paenibacillus, especially those from extreme environments that have been reported, are known for producing bioactive compounds with agricultural and biotechnological applications. In this study, we investigated the genomic and biochemical potential of Paenibacillus sp. VCA1 strain isolated from a thermophilic environment. Taxonomic identification was performed using whole genome similarity analysis, TETRA four-nucleotide frequency of occurrence analysis, ANI average nucleotide identity analysis, and gene distance analysis using digital DNA-DNA hybridization (dDDH). Functional analysis of the strain VCA1 was performed by detecting genes, enzymes, and genome subsystems involved in biocontrol and plant growth promotion,… More >

  • Open Access

    ARTICLE

    Application of Image Processing Techniques in Rice Grain Phenotypic Analysis and Genome-Wide Association Studies

    Jiexiong Xu
    Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2365-2383, 2025, DOI:10.32604/phyton.2025.067124
    (This article belongs to the Special Issue: Advances in Enhancing Grain Yield: From Molecular Mechanisms to Sustainable Agriculture)
    Abstract Background: Rice grain morphology—including traits such as awn length, hull color, size, and shape—is of central importance to yield, quality, and domestication, yet comprehensive quantification at scale has remained challenging. A promising solution has been provided by the integration of high-throughput imaging with genomic analysis. Methods: A standardized 2D image-processing pipeline was established to extract four categories of traits—awn length, hull color, projected grain area, and shape descriptors via PCA of normalized contours—from high-resolution photographs of 229 Oryza sativa japonica landraces. Genome-wide association analyses were then performed using a mixed linear model to control for population… More >

  • Open Access

    ARTICLE

    The Impact of Major Meteorological Factors in Tobacco Growing Areas on Key Chemical Constituents of Tobacco Leaves

    Guanhui Li, Jiati Tang, Qifang Zhang, Guilin Ou, Yingchao Lin, Liping Chen, Xiang Li, Shengjiang Wu, Zhu Ren, Zeyu Zhao, Xuekun Zhang, Benbo Xu, Xun Liu, Kesu Wei
    Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2385-2398, 2025, DOI:10.32604/phyton.2025.068213
    (This article belongs to the Special Issue: Advances in Enhancing Grain Yield: From Molecular Mechanisms to Sustainable Agriculture)
    Abstract To clarify the relationships between the main chemical components in flue-cured tobacco in Guizhou and field meteorological factors during the tobacco growing period, the contributions of meteorological factors to the chemical composition of flue-cured tobacco and related components were explored in this study. The flue-cured tobacco variety Y87 was used as the experimental material, and tobacco samples and meteorological data were collected from seven typical tobacco-growing areas in Guizhou Province. Using a random forest model and canonical correlation analysis, the impact and contribution of the monthly mean temperature, precipitation, and sunshine duration during the field… More >

  • Open Access

    REVIEW

    Application and Prospects of CRISPR/Cas Gene Editing Technology in Major Crop Molecular Breeding and Improving

    Dao Yao, Junming Zhou, Yashuo Wang, Yuxin Li, Wenge Cheng, Xiaoyu Lu, Huijing Liu
    Phyton-International Journal of Experimental Botany, Vol.94, No.6, pp. 1669-1694, 2025, DOI:10.32604/phyton.2025.064344
    (This article belongs to the Special Issue: Advances in Enhancing Grain Yield: From Molecular Mechanisms to Sustainable Agriculture)
    Abstract Clustered regularly interspaced short palindromic repeat sequences (CRISPR) and their accompanying proteins (Cas), commonly presenting in bacteria and archaea, make up the CRISPR/Cas system. As one of the fundamental sources of nutrition for humans, edible crops play a crucial role in ensuring global food security. CRISPR/Cas9 gene editing has been applied to improve many crop traits, such as increasing nitrogen utilization efficiency, creating male sterile germplasm, and regulating tiller and spikelet formation. This paper provides a comprehensive overview of the use of CRISPR/Cas gene editing technology in crop genomes, covering the targeted genes, the types More >

  • Open Access

    ARTICLE

    Drought-Ready Soybean Resilience: Molecular Marker-Based Screening and Selection of Superior Genotypes

    Muhammad Imran Khan, Fahad Masoud Wattoo, Kamran Shehzad Bajwa, Zahid Akram, Sabahat Noor, Zaheer Abbas, Rashid Mehmood Rana, Muhammad Tahir Akram, Shaukat Ali, Rai Muhammad Amir, Ghulam Muhammad Ali
    Phyton-International Journal of Experimental Botany, Vol.94, No.4, pp. 1299-1318, 2025, DOI:10.32604/phyton.2025.062323
    (This article belongs to the Special Issue: Advances in Enhancing Grain Yield: From Molecular Mechanisms to Sustainable Agriculture)
    Abstract The agricultural sector is notably affected by climate change, especially soybeans, which may face diminished yields because of severe water shortages. The evaluation of germplasm at morphological and molecular levels is an important pre-breeding step for crop improvement. This study employed 10 simple sequence repeat (SSR) markers to examine 60 soybean genotypes in the quest for drought-resistant lines during 2022–23. The results of the screening experiment (PEG-6000) revealed that the soybean genotypes SPS13, SPS195, PGRB83, and 39982 exhibited significant correlations in growth parameters. The results of molecular characterization indicated that five out of ten molecular… More >

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