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Antonios Chrysargyris^1,*, Demetris Antoniou², Timos Boyias², Nikolaos Tzortzakis^1,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.076565 - 30 January 2026
Abstract Cyprus has an extensive record in grape production and winemaking. Grapevine is essential for the economic and environmental sustainability of the agricultural sector, as it is in other Mediterranean regions. Intensive agriculture can overuse and exhaust natural resources, including soil and water. The current study evaluated how conservation strategies, including no tillage and semi-tillage (as a variation of strip tillage), affected grapevine growth and grape quality when compared to conventional tillage application. Two cultivars were used: Chardonnay and Maratheftiko (indigenous). Soil pH decreased, and EC increased after tillage applications, in both vineyards. Tillage lowered soil… More >

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Mario D’Ambrosio¹, Elisabetta Bigagli^1,*, Lorenzo Cinci¹, Cecilia Brunetti^2,*, Edgardo Giordani³, Francesco Ferrini³, Cristina Luceri¹
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.073250 - 30 January 2026
（This article belongs to the Special Issue: Plant-Derived Natural Products: Phytochemical Diversity and Functional Properties)
Abstract Moringa oleifera (MO) is traditionally used to mitigate inflammatory-mediated disorders; however, the influence of ecotypic variation on its anti-inflammatory activity remains poorly understood. In this study, we compared the phytochemical composition and anti-inflammatory activity of ethanolic extracts obtained from fresh and dried leaves of four MO ecotypes (India, Paraguay, Mozambique, and Pakistan), all grown under the same outdoor conditions, as well as two commercial powders (Just Moringa and WISSA), using LPS-stimulated RAW 264.7 macrophages. Extracts from fresh leaves were 19–43% more cytotoxic than those from dried leaves, depending on the ecotype, likely due to higher cyanogenic… More >

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Olga S. Tsiftsoglou^1,*, Vladimir Mihailovic², Nikola Sreckovic², Jelena S. Katanic Stankovic³, Kyriakos Michail Dimitriadis¹, Michalis K. Stefanakis⁴, Diamanto Lazari¹
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.075566 - 30 January 2026
（This article belongs to the Special Issue: Plant Responses and Adaptations to Environmental Stresses)
Abstract Achillea species are known for their healing properties since ancient times. There is extensive literature on their pharmacological action due to their bioactive compounds. The present study aimed to investigate the antioxidant and antimicrobial effects of hydroalcoholic extracts from the inflorescences and leaves of the species Achillea crithmifolia Waldst. and Kit., A. grandifolia Friv. and A. millefolium L. The phytochemical profiles of all extracts were evaluated both by NMR spectroscopy and LC-MS analysis, and the results were consistent with the spectrophotometrically determined total phenolic (TP: 125.42–191.98 mg/g) and total flavonoid (TF: 47.34–180.02 mg/g) contents. All the extracts were tested More >

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Olha Mykhailenko^1,2,3,#,*, Zigmantas Gudžinskas⁴, Liudas Ivanauskas⁵, Victoriya Georgiyants¹, Chia-Hung Yen^6,7,8, Chung-Fan Hsieh⁹, Riong Seulina Panjaitan⁶, Tsong-Long Hwang^10,11,12,13, Bing-Hung Chen^14,15, Michal Korinek^6,7,8,#,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.074209 - 30 January 2026
（This article belongs to the Special Issue: Secondary Metabolites in Plants and Their Interaction With Ecological Factors)
Abstract The sustainable sourcing of novel bioactive compounds from natural sources is crucial to the success of the pharmaceutical, food, and cosmetics industries. Iris bucharica Foster (syn. Juno bucharica (Foster) Vved.) is a promising source of novel bioactive molecules, particularly phenolic compounds, which are renowned for their antioxidant properties. In this study, we developed a reliable HPLC-UV-DAD method to identify and quantify phenolic compounds in the leaves and bulbs of I. bucharica, establishing the first set of quality control markers for this species. A total of 21 phenolic compounds were identified in the leaves, with flavonoids isoorientin, guaijaverin, hyperoside, More >

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87

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Wonkyu Yi, Jongseok Park^*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.073450 - 30 January 2026
（This article belongs to the Special Issue: Metabolic Mechanisms of Plant Responses to Stress)
Abstract Understanding plant responses under low-pressure conditions is important for developing closed cultivation systems that simulate space environments. This study aimed to assess the effects of different pressure levels on growth, photosynthesis, and secondary metabolite accumulation in red leaf lettuce (Lactuca sativa L. var. ‘Super Caesar’s Red’). Plants were cultivated for three weeks in sealed chambers under 101 kPa (atmospheric pressure), 66 kPa (moderate low pressure), and 33 kPa (severe low pressure). Growth analysis showed that leaf length and leaf area decreased significantly with reduced pressure, while chlorophyll content and SPAD values increased gradually. Photosynthetic measurements indicated More >

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Dmytro A. Kiriziy¹, Oksana V. Dubrovna¹, Oksana G. Sokolovska-Sergiienko¹, Alina S. Holoboroda¹, Victor V. Rohach^1,2, Oleg O. Stasik^1,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.075371 - 30 January 2026
（This article belongs to the Special Issue: Plant Responses to Abiotic Stress Mechanisms)
Abstract Water scarcity severely constrains the genetic potential of wheat yield worldwide. Proline is among the most versatile stress-related metabolites in plants, and targeting genes involved in proline synthesis and degradation represents a promising strategy for developing drought-tolerant wheat genotypes. This study evaluates the performance of the photosynthetic apparatus in transgenic wheat line with RNAi-mediated suppression of proline dehydrogenase (ProDH) and in the original (wild-type) genotype, under both drought and recovery conditions. Drought was induced at the flowering stage by lowering soil moisture to 30% field capacity for 7 days, compared with 70% field capacity in… More >

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Yun-Im Kang¹, Youn Jung Choi¹, Su Young Lee¹, Young-Ran Lee¹, Ki-Byung Lim^2,3, Yun-Jae Ahn^2,3,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.073138 - 30 January 2026
（This article belongs to the Special Issue: Plant Responses to Stress Factors)
Abstract Cucumber mosaic virus (CMV) threatens lily production by reducing floral quality and enabling carry-over via infected planting stock. To explore tissue-specific host responses, we analyzed a legacy, single-replicate RNA-seq dataset from two cultivars, ‘Cancun’ and ‘Connecticut King’ (CK), profiling leaf (source) and bulb (sink) tissues at 0 and 28 days post-inoculation (dpi), alongside leaf DAS-ELISA. Principal component analysis indicated that tissue identity dominated the transcriptome (PC1 = 47.7%), with CMV treatment driving within-tissue shifts over time. Exploratory Gene Ontology/KEGG summaries and a focused marker panel revealed a consistent split: in leaves, genes linked to jasmonate/WRKY-associated… More >

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Dilfuza Jabborova^1,2,3,*, Khurshid Sulaymanov¹, Muzafar Jabborov⁴, Nayan Ahmed⁵, Tatiana Minkina⁶, Olga Biryukova⁶, Nasir Mehmood^6,*, Vishnu D. Rajput⁶
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.072577 - 30 January 2026
（This article belongs to the Special Issue: Integrated Nutrient Management in Cereal Crops)
Abstract The combined effects of macronutrients (Nitrogen, Phosphorus, and Potassium-N, P, K) and micronutrient fertilization on turmeric yield, soil enzymatic activity, microbial biomass, and nutrient dynamics remains poorly understood, despite their significance for sustainable soil fertility management and optimizing crop productivity across diverse agroecosystems. To investigate, a net house experiment on sandy loam Haplic Chernozem was conducted to 03 fertilizer regimes, viz. N₇₅P₅₀K₅₀ kg ha⁻¹ (T-2), N₁₂₅P₁₀₀K₁₀₀ kg ha⁻¹ (T-3), and N₁₀₀P₇₅K₇₅ + B₃Zn₆Fe₆ kg ha⁻¹ (T-4). Furthermore, the influence of these treatments was systematically assessed on soil nutrient status (N, P, K), enzymatic activities (alkaline phosphomonoesterase, dehydrogenase, fluorescein diacetate… More >

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Ziying Huang¹, Ruikang Ma¹, Anning Li², Yufei Cheng¹, Xiaolin Lin², Mengzhi Li³, Yu Zhang², Liping Shi¹, Linlin Dong^1,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.074419 - 30 January 2026
Abstract Epimedium is commonly used to treat bone injury and kidney disease, with prenylated flavonol glycosides (PFGs) as its active ingredients. It has attracted much attention due to prominent healthcare and therapeutic effects, but faces problems of adulteration with closely related species and confusion about geographical origins. In this study, multiple technical approaches were employed to identify its genetic characteristics and metabolic differences. Based on DNA barcoding, 20 batches of samples were analyzed. The genetic distances of matK, ITS and psbA-trnH within species were all smaller than those between species, and psbA-trnH along with ITS + psbA-trnH proved most effective… More >

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237

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Adolfo Amador Mendoza^1,*, Rosalba Guadalupe Gomez Raymundo², Ana Rosa Ramírez Seañez¹, Hipolito Hernández Hernández¹, Rogelio Enrrique Palacios Torres¹, Nelda Xanath Martínez Galero³, Miguel Ángel García Muñoz³, Saribel Zilli Gutiérrez^4,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.073856 - 30 January 2026
（This article belongs to the Special Issue: Endophytic Microbiota: Prospects and Challenges for Application Towards Sustainable Agriculture and Environmental Management)
Abstract The cultivation of Soledad pepper (Capsicum annuum L.) is essential in Oaxaca and Veracruz, but it faces issues with pests and diseases, which affect yield and cause economic losses. To mitigate these impacts, farmers have started using biostimulants such as chitosan and plant growth promoting bacteria instead of agrochemicals due to their environmental and health benefits. This study evaluated the effect of Bacillus subtilis and chitosan, both individually and combined, on the growth, yield, and fruit quality of Soledad pepper under greenhouse conditions. Four treatments were applied at different stages of the crop cycle: Q (Chitosan), BS (Bacillus More >

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Hong Chen^1,2, Runze Yang^1,2, Jing Tian^1,2, Boyuan Xu^1,2, Qiang Chen³, Yuzong Chen^1,2, Ming-Xiao Zhao^1,2,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.072276 - 30 January 2026
（This article belongs to the Special Issue: Fungal and Bacterial Disease Management in Agricultural Crops Through Biological Control, Disease Resistance, and Transcriptomics Approaches)
Abstract Panax species are globally recognized for their high medicinal and economic value, yet large-scale cultivation is constrained by high production costs, progressive soil acidification, and persistent soil-borne diseases. Although various soil improvement strategies have been tested, a comprehensive synthesis of their comparative effectiveness has been lacking. Here, we conducted a meta-analysis of 1381 observations from 54 independent studies to evaluate the effects of conventional fertilizers, microbial fertilizers, organic amendments, and inorganic amendments on Panax cultivation. Our results demonstrate that microbial fertilizers, organic amendments, and inorganic amendments significantly increased soil pH, thereby ameliorating soil acidification. Among them,… More >

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Amine Batbat^1,2, Khaoula Habbadi², Mohamed Jeddi³, Samiah Hamad Al-Mijalli⁴, Hanae Naceiri Mrabti⁵, Fahad M. Alshabrmi⁶, Naif Hesham Moursi⁷, Hassane Greche¹, Naoufal El Hachlafi^8,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.072398 - 30 January 2026
（This article belongs to the Special Issue: Innovative Strategies in Medicinal Plant Biotechnology: From Traditional Knowledge to Modern Applications)
Abstract Origanum elongatum (OE) is an aromatic, medicinal plant endemic to Morocco that is widely used in traditional medicine due to its biological properties. This study aimed to elucidate the chemical composition of the essential oil (EO) obtained from O. elongatum (OEEO) at three stages of its life cycle, including vegetative stage (OEEO-VS), flowering stage (OEEO-FS), and post-flowering (OEEO-PFS), as well as to evaluate its biological and antiradical characteristics. The chemical analysis of the essential oil was conducted using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity was evaluated in vitro through distinct methodologies, namely, disc diffusion and microatmosphere assay;… More >

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Lu Chen^1,2,#, Zhijie Ren^2,#, Guangmin Zhao², Xuan He², Legong Li², Sheng Teng^1,*, Yikun He^2,*, Fang Bao^2,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.073817 - 30 January 2026
（This article belongs to the Special Issue: Plant Responses to Abiotic Stress Mechanisms)
Abstract ERD4 proteins, members of the early responsive-to-dehydration family, act as plasma membrane ion channels that contribute to ion homeostasis and modulate plant response to abiotic stresses. However, the functions of ERD4 homologs in non-vascular species remain largely unexplored. Here, we characterized an ERD4 family homolog in Physcomitrium patens (Hedw.) Mitt., PpCSC1 (Calcium-permeable Stress-responsive Cation Channel 1), and investigated its role in salt stress response. PpCSC1 localized to the plasma membrane and functioned as a non-selective cation channel permeable to Na⁺, K⁺, Ca²⁺, and Mg²⁺. Under salt treatment, PpCSC1 transcripts were markedly downregulated, whereas overexpression lines exhibited enhanced salt sensitivity.… More >

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192

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Aaron David Lugo-Palacios¹, Edgar Omar Rueda-Puente², César Omar Montoya-García², Ignacio Orona-Castillo³, Urbano Nava-Camberos³, José Luis García-Hernández^3,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.075449 - 30 January 2026
Abstract To ensure the efficient use of resources, particularly in water-scarce arid and semi-arid regions where abiotic stress threatens food security, assessing soil and climate suitability for specific crops is crucial. Simultaneously, food production must align with sustainable development goals by minimizing negative environmental impacts. Therefore, establishing agro-climatic suitability using a spatiotemporal approach is essential. This involves three key steps: first, determining the climatically appropriate months based on the species’ requirements (temporal suitability), and second, establishing the soil suitability of specific plots (spatial suitability). Following this, quantifying crop evapotranspiration allows for optimized water use. This study… More >

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Jiazhou Li^1,2, Mingyu Zhang¹, Xing Li^1,3, Fangbo Cao^1,2, Jiana Chen^1,2, Weiqin Wang^1,2, Huabin Zheng^1,2, Min Huang^1,2,4,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.075255 - 30 January 2026
Abstract Spikelet filling characteristics in early-season rice in southern China may be distinctive due to its exposure to high temperatures during the ripening period. However, limited information is currently available on these characteristics. This study aimed to characterize spikelet filling in early-season rice and identify the key factors contributing to its improvement. Field experiments were conducted over two years (2021 and 2022) to mainly investigate the proportions of fully-filled, partially-filled, and empty spikelets, along with the biomass-fertilized spikelet ratio and harvest index, in 11 early-season rice varieties. The results revealed significant varietal variation in spikelet filling,… More >

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Hasan Kaan Kucukerdem^*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.074188 - 30 January 2026
（This article belongs to the Special Issue: Application of Digital Agriculture and Machine Learning Technologies in Crop Production)
Abstract Root-zone temperature (RZT) strongly affects plant growth, nutrient uptake and tolerance to environmental stress, making its regulation a key challenge in greenhouse cultivation in cold climates. This study aimed to assess the potential of passive techniques, namely black polyethylene mulch and row covers, for modifying RZT dynamics in lettuce (Lactuca sativa L.) production and to evaluate the predictive performance of the eXtreme Gradient Boosting (XGBoost) algorithm. Experiments were conducted in Iğdır, Türkiye, over a 61-day period, with soil temperature continuously monitored at depths of 1–30 cm under mulched and non-mulched conditions, alongside measurements of greenhouse air… More >

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Meng Wang¹, Jinghan Cai¹, Wenzheng Liu¹, Xue Yang¹, Jingjing Zhang¹, Qiangmin Zhou¹, Fanzhen Wang¹, Hang Zhang^1,*, Tonghai Liu^2,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2025.075541 - 30 January 2026
（This article belongs to the Special Issue: Plant Protection and Pest Management)
Abstract Tomato is a major economic crop worldwide, and diseases on tomato leaves can significantly reduce both yield and quality. Traditional manual inspection is inefficient and highly subjective, making it difficult to meet the requirements of early disease identification in complex natural environments. To address this issue, this study proposes an improved YOLO11-based model, YOLO-SPDNet (Scale Sequence Fusion, Position-Channel Attention, and Dual Enhancement Network). The model integrates the SEAM (Self-Ensembling Attention Mechanism) semantic enhancement module, the MLCA (Mixed Local Channel Attention) lightweight attention mechanism, and the SPA (Scale-Position-Detail Awareness) module composed of SSFF (Scale Sequence Feature… More >

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Kirti A. Yadav¹, Yachana Jha¹, Haiam O. Elkatry², Heba I. Mohamed^3,*, Ahmed Mahmoud Ismail⁴, Abdelrahman R. Ahmed^2,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.074570 - 30 January 2026
（This article belongs to the Special Issue: Fungal and Bacterial Disease Management in Agricultural Crops Through Biological Control, Disease Resistance, and Transcriptomics Approaches)
Abstract Phytophthora blight is a devastating disease of pigeon pea (Cajanus cajan) that severely impacts plant growth and productivity. This study investigates the morphological, anatomical, and biochemical responses of a susceptible variety (ICPL 11260) and a resistant variety (IPAC-02) following infection by Phytophthora. Morphological analyses showed that infection caused a drastic reduction in root length, shoot length, leaf number, fresh weight, and dry weight in the susceptible ICPL 11260 variety, with reductions ranging from 0.5- to 2-fold compared to non-infected controls. Anatomical observations revealed pronounced cellular damage and mycelial invasion in infected ICPL 11260 plants by 30… More >

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Zakia Akter¹, Sumona Akter Jannat², Sheikh Md. Shibly¹, Afroza Sultana¹, Amdadul Hoque Amran¹, Joairia Hossain Faria¹, Sabina Yeasmin¹, Md. Parvez Anwar^1,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.075524 - 30 January 2026
（This article belongs to the Special Issue: Utilization of Biostimulants in Plant Growth and Health)
Abstract Seaweed extract contains plant growth regulators and bio-stimulants that enhance plant growth and development. In Bangladesh, winter rice (Boro rice) in the nursery bed often shows poor seed emergence and weak seedling growth due to low temperature. This problem can be addressed by using seaweed extract as a seed priming agent and bio-stimulant. The objective of this study was to evaluate the effectiveness of seaweed extract (Crop Plus) on seed emergence, seedling growth, and vigor of winter rice in the nursery. Two experiments were conducted at Bangladesh Agricultural University using BRRI dhan89. The laboratory experiment… More >

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Xiaoqing Jiang^1,2,#, Pengchong Li^1,2,#, Hongchuang Liu^1,2, Wenjie Dong^1,2, Wenjing Liu^1,2, Di Wu^1,2, Jianping Xue^1,2, Fenglan Zhao^1,2,*, Yongbo Duan^1,2,*
Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.074434 - 30 January 2026
Abstract As its tuberous alkaloids induce valuable pharmacological effects, Pinellia ternata has considerable clinical value. However, its production currently fails to meet its demand. In vitro microtuber culture, combined with salicylic acid (SA) elicitation, may provide an effective alternative to traditional field production. In this study, an in vitro P. ternata microtuber induction system was developed and used to evaluate SA-induced elicitation of alkaloid accumulation. The quality of in vitro microtubers was assessed by total alkaloid measurement, a cytotoxicity assay, and transcriptomic analysis. With or without SA treatment, P. ternata microtuber induction was achieved within 60 d using petiole-derived explants, with… More >

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