Dairong LI¹, Xianlu ZHUO^1,2, Lumi HUANG¹, Xiaohui JI¹, Donglin WANG¹

BIOCELL, Vol.43, No.3, pp. 139-144, 2019, DOI:10.32604/biocell.2019.06460

Abstract X-ray repair cross-complementing protein 1 (XRCC1) could repair cisplatin-induced DNA damage. XRCC1 Arg399Gln and Arg194Trp variants alter XRCC1 expression and function, leading to changes in cancer sensitivity to cisplatin treatment. This study aimed to investigate the effects of XRCC1 Arg399Gln and Arg194Trp polymorphisms on cell viability, apoptosis and XRCC1 expression in cisplatin-sensitive A549 and cisplatin-resistant A549/DDP nonsmall cell lung cancer (NSCLC) cells. Plasmids carrying XRCC1 Arg399Gln and Arg194Trp were constructed and transfected into A549 and A549/DDP cells. RT–PCR, Western blot, MTT assay, and flow cytometry analysis were performed to assess cell viability, apoptosis, and XRCC1 expression. Compared to control cells,… More >

Wen JING^1,§, Chuanfen LI^2,3,§, Yingli LU⁴, Lianshi FENG⁴

BIOCELL, Vol.43, No.3, pp. 129-138, 2019, DOI:10.32604/biocell.2019.06087

Abstract This study aimed to investigate the microRNA expression profile and the characteristics of lipid metabolism in the livers of rats undergoing a high-fat diet. Fifty male Sprague-Dawley (SD) rats were divided into a standard chow group (C group, N = 10) and a high-fat diet group (H group, N = 40). After 12 weeks, the rat body weight, body length, fat mass, and serum lipid concentration were measured. The expression profile of microRNAs and the gene and protein expression levels involved in lipid metabolism in rat liver were detected. Body fat and serum lipid concentrations were all significantly higher in… More >

Ji SHI; Peixin SUN, Ye ZHANG, Bing YAO, Haozhe PIAO

BIOCELL, Vol.43, No.3, pp. 119-128, 2019, DOI:10.32604/biocell.2019.07577

Abstract Glioma is the most common primary malignant brain tumor with a poor survival rate. In recent years, no significant progress has been made in the treatment of gliomas in contrast to the development of improved diagnosis via molecular typing. Newcastle disease virus (NDV), a negative-stranded RNA virus that exhibits oncolytic activity, has been investigated for its capacity to elicit antitumor activity in many types of cancers, including glioma. Therefore, application of oncolytic viruses, such as NDV, as a new treatment strategy to specifically target aberrant signaling in glioblastomas has brought new hope. For many years, NDV has been investigated for… More >

Bengü ERGÜDEN

BIOCELL, Vol.43, No.3, pp. 103-118, 2019, DOI:10.32604/biocell.2019.06517

Abstract The expression of a gene is governed at various levels, from transcriptional to translational level. The translational control is widely used to regulate gene expression, especially when a rapid, local, and selective control over protein synthesis is required. The present review describes instructive examples of translational regulation in yeast, together with regulatory elements within mRNAs. The review also outlines the important contributions of mRNAbinding proteins that act in harmony with several translational elements to generate appropriate translational signals and responses. More >

Zongqiang GAO¹, Xiong GUO², Junchang CHEN¹, Chen DUAN²

BIOCELL, Vol.43, No.2, pp. 99-102, 2019, DOI:10.32604/biocell.2019.06520

Abstract This study aimed to investigate the effect of hyaluronic acid (HA) on the expression of heat shock protein 70 (HSP70) in chondrocytes isolated from patients with osteoarthritis (OA) and Kashin-Beck disease (KBD). The chondrocytes were collected from OA and KBD patients, and chondrocytes isolated from patients of accident injuries were used as the control. The chondrocytes were treated with HA at different doses. HSP70 expression in chondrocytes at both mRNA and protein levels was tested by PCR and Western blot analysis. Compared with control, both mRNA and protein levels of HSP70 were higher in chondrocytes from KBD and OA. However,… More >

Xiaoning Qin¹, Liqing Yuan², Hongxun Ruan¹, Lin Lin¹

BIOCELL, Vol.43, No.2, pp. 89-98, 2019, DOI:10.32604/biocell.2019.06352

Abstract Interleukin-22 (IL-22) is a member of IL-10 cytokine family which is expressed in activated T cells predominantly and in activated natural killer cells at lower levels. Previous studies have demonstrated the link between elevated levels of IL-22 and disease severity of psoriasis, Crohn’s disease, rheumatoid arthritis and interstitial lung diseases. However, the function of IL-22 in the development and progression of colorectal cancer (CRC) remains elusive. In this study, we first evaluated the IL-22/IL-22R1 level in CRC patients, and found that tumor tissues have more active expression of IL-22 and IL-22R1 than normal tissues, presenting correlation with the degree of… More >

Mahmoud MOUSTAFA^1,2, Saad ALAMRI¹, Hoida ZAKI², Naglaa LOUTFY², Tarek TAHA³, Ali SHATI¹, Mohamed ALKAHTANI¹, Sajda SIDDIQI¹

BIOCELL, Vol.43, No.2, pp. 81-88, 2019, DOI:10.32604/biocell.2019.06231

Abstract The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases, cytoskeletal proteins, ATPases, ADPase and amount of amino acid of pea (Pisum sativum L. var. Alaska). Pellets of cytoskeletals proteins (27000 xg) were probed with anti-apyrase antibody, biotinylated anti-rat, actin and alpha and beta-tubulin for Western blotting. ATPase and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine diphosphate. By 72 hours, the abundance of apyrases, cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds… More >

Entsar A. SAAD¹, Reda S. EL-DEMERDASH², Eman M. ABD EI-FATTAH¹

BIOCELL, Vol.43, No.2, pp. 73-80, 2019, DOI:10.32604/biocell.2019.07020

Abstract Cisplatin is a powerful anticancer drug but its nephrotoxic effects limit its clinical use. We aimed to evaluate the effect of mesenchymal stem cells (MSCs) injection or of captopril to counteract the cisplatin-induction of nephropathy. MSCs isolation, preparation and tracking, transforming growth factor-β (TGF-β) and interleukin-10 (IL-10) expressions, kidney function tests, oxidative stress state, and histological examinations were done. Cisplatininduced nephropathy was indicated biochemically and confirmed histopathologically. MSCs treatment showed normal kidney architecture, and significantly decreased oxidative stress and TGF-β while increased IL-10 and improved kidney function tests. Rats treated with cisplatin + captopril showed noticeable kidney histopathological changes. Superior… More >

Ramesa Shafi BHAT¹, Amina El GEZEERY², Abir Ben BACHAN¹, Mona Awad ALONAZI¹, Leena Saleh ALSUHAIBANI¹, Afaf El-ANSARY^3,4

BIOCELL, Vol.43, No.2, pp. 65-71, 2019, DOI:10.32604/biocell.2019.07012

Abstract Fluoride is a key ingredient of many psychiatric drugs like fluoxetine (Prozac^®, Fluoxetine^®). Pregnant women frequently use this drug as they suffer from depression and anxiety disorders during this period. Fluoxetine is able to reach the fetus through the placenta and passes to the newborn through milk. In the present study, female Wistar rats were treated with 5, 10, and 20 mg/L fluoxetine (containing 94% fluorides) from pregnancy day 10 to day 20. After delivery, the levels of the enzymatic antioxidants in the brain of their offspring at postnatal day 2 were measured. The results showed that, in all fluoxetine… More >

HAIFENG WANG^1,2, KAI WANG¹, JUAN GUO¹, TIEQIAO WEN²

BIOCELL, Vol.43, No.2, pp. 59-64, 2019, DOI:10.32604/biocell.2019.05731

Abstract Histone deacetylation is a key modulator involved in cell proliferation, apoptosis, and mRNA transcription. However, the effects of histone deacetylation on C17.2 neural stem cells (NSCs) remain unclear. Here, the histone deacetylase inhibitors nicotinamide and trichostatin A (TSA) were used to determine the role of histone deacetylation on gene transcription in NSCs. The results showed that the mRNA expression of p53, Sox1, Sox2, and Bax were significantly higher in E14.5 NSCs than in C17.2 NSCs. Nestin, a marker gene of neuronal differentiation, did not differ significantly between E14.5 NSCs and C17.2 NSCs. The transcription levels of p53 and Nestin were… More >