YAPING WANG^1,2,#,*; XIAOHONG XUE^2,#; ZHEN ZHAO^1,#; XIAOLIN LI²; ZHIYONG ZHU¹

BIOCELL, Vol.46, No.7, pp. 1651-1659, 2022, DOI:10.32604/biocell.2022.018559

Abstract The pathogenesis of high altitude-related gastric mucosal injury remains poorly understood, this study aimed to investigate the role of autophagy in hypoxia-induced apoptosis of rat gastric mucosal cells. Rats were randomized into four groups which were maintained at an altitude of 400 m (P) or received no treatment (H), autophagy inducer rapamycin (H+AI) or autophagy inhibitor 3-MA (H+AB) at an altitude of 4,300 m for 1, 7, 14 and 21 days, respectively, and the morphology, ultrastructure, autophagy, and apoptosis of gastric mucosal tissues were examined. Gastric mucosal epithelial cells CC-R039 were cultured under conditions of normoxia, 2% O2 (hypoxia), or… More >

XINFU FAN^1,2,#, JIE CHEN^3,#, ZONGMENG ZHANG^4,#, FANGHUI CHEN⁵, HAOSEN WANG⁶, YAFEI CAI⁶, JUN LI^7,*

BIOCELL, Vol.46, No.6, pp. 1527-1535, 2022, DOI:10.32604/biocell.2022.018269

Abstract Spinal cord injury (SCI) remains an intractable clinical challenge of neurosurgery, it can be divided into two stages: uncontrollable primary injury induced by mechanical damage and controllable secondary injury regulated by continuous cell death. The apoptosis was the one of most important events in secondary injury, previous studies revealed that excessive endoplasmic reticulum (ER) stress breaks down the homeostasis and triggers apoptosis in the spinal cord. To deter or alleviate the secondary jury, we screen one of fat-soluble compounds, salubrinal, which was an inhibitor of eIF2α dephosphorylation can repair SCI by inhibiting ER stress in mice after SCI. Administration of… More >

Ping Zhao^1,#, Yao Xu^1,#, Anqi Wu², Yuhao Hu², Aiting Cai², Xinyue Wang³, Feng Wang^2,*, Xiang Chen^1,*

Oncologie, Vol.23, No.4, pp. 589-601, 2021, DOI:10.32604/oncologie.2021.018713

Abstract Background: Although there has been a part of research that the expression of circular RNAs (circRNAs) is bound up with the occurrence, development and prognosis of multiple tumors, what roles circRNAs have in hepatocellular carcinoma (HCC) remain to be explored. Methods: Hsa_circ_0016863 showed a trend of high expression in HCC tissues by Microarray GSE97332. The expression of hsa_circ_0016863 in HCC and adjacent non-cancerous tissues was detected by qRT-PCR. The role of hsa_circ_0016863 in HCC was investigated by colony formation assay, cell proliferation assay, Transwell assay and flow cytometry. Results: Hsa_circ_0016863 exhibited a trend of high expression in HCC. Cell experiments… More >

LONGJU QI^1,2,#, XIAOYING XU^3,#, BIN LI^4,#, BO CHANG⁵, SHENGCUN WANG², CHUN LIU², LIUCHENG WU², XIAODI ZHOU⁴, QINGHUA WANG^2,*

BIOCELL, Vol.46, No.4, pp. 989-998, 2022, DOI:10.32604/biocell.2022.018014

Abstract Excessive fat ectopically deposited in the non-adipose tissues is considered as one of the leading causes of myopathy. The aim of this study was to investigate the role of Dihydroartemisinin (DHA) in palmitate (PAL)-incubated H9c2 cells (lipotoxicity-induced cell injury model). Cell viability of PAL-treated cells was determined by MTT assay, and apoptotic regulators were examined by qRT-PCR and western blot analysis, in the absence or in the presence of DHA, respectively. Expression levels of miR-133b and Sirt1 were also evaluated by qRT-PCR and western blotting examination. PAL decreased the viability of H9c2 cells and enhanced the expression of apoptotic genes.… More >

TIANTIAN XU^1,#, RUILIN ZHENG^1,#, DANYANG CHEN^1,#, HAIYANG CHEN², MINGQI ZHAO¹, MIN GUO¹, YI CHEN¹, CHANGBING WANG¹, LU KUANG¹, YINGHUA LI^1,*, BING ZHU^1,*

BIOCELL, Vol.46, No.4, pp. 1033-1039, 2022, DOI:10.32604/biocell.2022.017757

Abstract Emerging evidence that Enterovirus A 71 (EV-A71) infection closely related to apoptosis. The ZVAD is a caspase inhibitor that can prevent apoptosis. The aims of this project were to evaluate the mechanism of the ZVAD inhibited EV-A71 virus and to provide experiment basis for finding new antiviral drugs. In this study, after treated with ZVAD in EV-A71 infected Vero cells, the viral replication was reduced, and the cell viability was higher than EV-A71 group. Additionally, ZVAD decreased the cell apoptosis and the level of inflammatory cytokines induced by EV-A71 in the infected Vero cells. ZVAD inhibited cell apoptosis by regulating… More >

XIANGCAI YANG¹, JIAGUI QU², JIEJING LI^1,*

BIOCELL, Vol.46, No.4, pp. 1041-1051, 2022, DOI:10.32604/biocell.2022.017383

Abstract Malignant glioma is one of the most common and deadly tumors in the central nervous system while developing effective treatments for this devastating disease remains a challenge. Previously, we demonstrated that the vitamin nicotinic acid (NA) inhibits glioma invasion. Here, we show that high-dose NA induces apoptosis of malignant glioma cells in vitro and in vivo. In cultured U251 glioma cells treated with NA, we detected ER stress that was likely caused by elevated intracellular calcium levels. The elevated calcium can be attributed to the activation of TRPV1, a cation channel that has been implicated in cutaneous flushing caused by… More >

MENGTING LI^*, YI-ER QIU, KAIFENG ZHENG

BIOCELL, Vol.46, No.4, pp. 1089-1096, 2022, DOI:10.32604/biocell.2022.013432

Abstract The effect of the baicalin, a bio-active flavonoid extracted from Scutellaria baicalensis Georgi, on the carbon tetrachloride (CCl₄) induced liver fibrosis was investigated. To compare the effect of baicalin on the liver fibrosis, five different groups of rats treated by 100, 200, and 400 mg/kg baicalin were studied. Upon CCl₄ treatment, the levels of procollagen type III, aspartate aminotransferase, aminotransferase, hyaluronic acid, and hydroxyproline were significantly increased, whereas the superoxide dismutase and glutathione peroxidase content were decreased. These changes in the biochemical parameters, which are associated with liver function, were significantly attenuated by the baicalin treatment, suggesting that baicalin can… More >

FEI ZHANG^1,2,#, JIANZHONG LAI^3,#, RONGHAN HE^1,#, YI SHI⁴, KUN XU⁵, SHIHAI JIANG¹, TANGZHAO LIANG¹, WANYU ZHAO⁵, WEIDA REN⁵, LEI ZHU⁶, SONG JIN^2,*, KUN WANG^1,*

BIOCELL, Vol.46, No.3, pp. 699-709, 2022, DOI:10.32604/biocell.2022.015726

Abstract Circular RNAs (circRNAs) are a novel class of non-coding RNA that have recently shown to have huge capabilities in the regulation of gene expression at the posttranscriptional level. Growing evidence has indicated that circRNAs could serve as competing endogenous RNAs (ceRNAs) to sponge microRNAs (miRNAs) and suppress functions of targeted miRNAs. Osteosarcoma (OS) is the most common malignant primary bone cancer. Hsa_circ_0002137 is upregulated in OS. However, the role of hsa_circ_0002137 in OS remains unclear. Using miRNA pull-down assay, we showed that cir_0002137 sponged hsa-miR-1246, and BCL2 apoptosis regulator (BCL2) mRNA was a potential target of hsa-miR-1246 in human osteosarcoma… More >

SHADY G. EL-SAWAH^1,*, FAYEZ ALTHOBAITI², HANAN M. RASHWAN¹, ADIL ALDHAHRANI³, MARWA A. ABDEL-DAYEM⁴, EMAN FAYAD², REHAB M. AMEN⁵, EL SHAIMAA SHABANA⁶, EHAB I. EL-HALLOUS⁷

BIOCELL, Vol.46, No.3, pp. 745-757, 2022, DOI:10.32604/biocell.2022.017853

Abstract Since Type 1 diabetes (T1DM) occurs when β-cells mass is reduced to less than 20% of the normal level due to autoimmune destruction of cells resulting in the inability to secrete insulin, preservation or replenishment of the functional β-cells mass has become a major therapeutic focus for this diabetic type treatment. Thus, this 4-week work plan was designed to determine which mesenchymal stem cells (MSCs) type is more appropriate to alleviate pancreatic hazards resulting from diabetes induction; via tracking a comparative study between MSCs derived from adipose tissue (AD-MSCs) and from bone marrow (BM-MSCs) in management of T1DM considering their… More >

XINWEI WU¹, GUOYONG JIA^2,*, HONGNA YANG³, CONGCONG SUN², YING LIU², ZENGYAN DIAO²

BIOCELL, Vol.46, No.2, pp. 471-478, 2022, DOI:10.32604/biocell.2021.015701

Abstract A progressive neurodegenerative disease, Alzheimer’s disease (AD). Studies suggest that highly expressed protein isoaspartate methyltransferase 1 (PCMT1) in brain tissue. In the current study, we explored the effects of neural stem cell-conditioned medium (NSC-CDM) on the PCMT1/MST1 pathway to alleviate Aβ_25-35-induced damage in SH-SY5Y cells. Our data suggested that Aβ25-35 markedly inhibited cell viability. NSC-CDM or Neural stem cell-complete medium (NSC-CPM) had a suppression effect on toxicity when treatment with Aβ_25-35, with a greater effect observed with NSC-CDM. Aβ_25-35 + NSC-CDM group exhibited an increase in PCMT1 expression. sh-PCMT1 markedly decreased cell proliferation and suppressed the protective role of NSC-CDM… More >