Weiliang Qi^1,2,3,4,*, Wancang Sun⁵, Li Ma⁵, Xiaolong Li¹, Haiqing Liu¹, Cairong Yang⁶, Ziyao Wei¹

Phyton-International Journal of Experimental Botany, Vol.94, No.3, pp. 739-762, 2025, DOI:10.32604/phyton.2025.060712 - 31 March 2025

Abstract Brassica napus L. (B. napus), recognized as a significant cash and oil crop, faces challenges in popularization and application in northern China due to its limited cold resistance. Clarifying the mechanism of cold stress on gene regulation and signal transduction in B. napus is crucial. To address these issues, we conducted transcriptome sequencing and gene expression analysis, along with gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway profiling under natural (25°C) and cold (4°C) conditions in cold tolerant 16VHNTS309 and weak cold-resistant Tianyou 2238 B. napus seedlings. Enhanced genomic annotation was achieved through additional sequencing.… More >

Sha Luo¹, Shuang Wu¹, Junyan Song¹, Xuena Yu¹, Kehao Liang¹, Zhi Huang¹, Yi Tang¹, Huanxiu Li¹, Victor Hugo Escalona², Zhifeng Chen³, Fen Zhang^1,*, Bo Sun^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.2, pp. 521-538, 2025, DOI:10.32604/phyton.2025.061099 - 06 March 2025

Abstract This study conducted karyotype analysis on 56 Chinese kale varieties from Guangdong and Fujian provinces using conventional chromosome analysis methods. The varieties were categorized into four groups based on their origin and flower color: white-flower Chinese kale originating from Guangdong (GW), yellow-flower Chinese kale originating from Guangdong (GY), white-flower Chinese kale originating from Fujian (FW), and yellow-flower Chinese kale originating from Fujian (FY). Karyotype differences among the four groups of Chinese kale were analyzed, and the evolutionary relationship between yellow-flower and white-flower Chinese kale from the two regions was inferred based on karyotype parameters. The… More >

Chengxu Qian¹, Qiangwen Chen^1,2, Leiyu Jiang¹, Xiaoyan Yang³, Shen Rao⁴, Weiwei Zhang¹, Feng Xu^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.5, pp. 951-962, 2024, DOI:10.32604/phyton.2024.050893 - 28 May 2024

Abstract The objective of this study was to explain the physiological mechanisms through which NaSeO mitigates the growth and developmental inhibition of pakchoi under HgCl stress. The results showed that treatment with HgCl (40 mg L) led to reduced biomass, dwarfing, root shortening, and root tip necrosis in pakchoi. Compared to control (CK), the activities of superoxide dismutase (SOD) and peroxidase (POD) in Hg treatment increased, and the content of malondialdehyde (MDA) also dramatically increased, which negatively impacted the growth of pakchoi. Low concentrations of NaSeO (0.2 mg L) significantly increased the content of soluble sugars… More >

Haoxue Wu^#, Xiaohan Zhang^§,#, Xiaoyu Chen, Kang Li, Aixia Xu, Zhen Huang, Jungang Dong, Chengyu Yu^*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 627-640, 2024, DOI:10.32604/phyton.2024.050321 - 28 March 2024

Abstract Genetic manipulation (either restraint or enhancement) of the biosynthesis pathway of α-linolenic acid (ALA) in seed oil is an important goal in Brassica napus breeding. B. napus is a tetraploid plant whose genome often harbors four and six homologous copies, respectively, of the two fatty acid desaturases FAD2 and FAD3, which control the last two steps of ALA biosynthesis during seed oil accumulation. In this study, we compared their promoters, coding sequences, and expression levels in three high-ALA inbred lines 2006L, R8Q10, and YH25005, a low-ALA line A28, a low-ALA/high-oleic-acid accession SW, and the wildtype ZS11. The expression… More >

Zain Anwar Ali^1,2,*, Dingnan Deng¹, Muhammad Kashif Shaikh³, Raza Hasan⁴, Muhammad Aamir Khan²

Computer Systems Science and Engineering, Vol.48, No.1, pp. 1-34, 2024, DOI:10.32604/csse.2023.041866 - 26 January 2024

Abstract Technological advances in unmanned aerial vehicles (UAVs) pursued by artificial intelligence (AI) are improving remote sensing applications in smart agriculture. These are valuable tools for monitoring and disease identification of plants as they can collect data with no damage and effects on plants. However, their limited carrying and battery capacities restrict their performance in larger areas. Therefore, using multiple UAVs, especially in the form of a swarm is more significant for monitoring larger areas such as crop fields and forests. The diversity of research studies necessitates a literature review for more progress and contribution in… More >

Liang Chai^1,2, Cheng Cui¹, Benchuan Zheng¹, Jinfang Zhang¹, Jun Jiang¹, Haojie Li^1,2,*, Liangcai Jiang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 645-663, 2023, DOI:10.32604/phyton.2023.024717 - 29 November 2022

Abstract Nitrogen (N) is one of the basic nutrients and signals for plant development and deficiency of it would always limit the productions of crops in the field. Quantitative research on expression of N-stress responsive proteins on a proteome level remains elusive. In order to gain a deep insight into the proteins responding to nitrogen stress in rapeseed (Brassica napus L.), comparative proteomic analysis was performed to investigate changes of protein expression profiles from the root, stem and leaf under different N concentrations, respectively. More than 200 differential abundance proteins (DAPs) were detected and categorized into groups More >

Yanhua Li^1,#, Hualei Huang^1,#, Youming Shi¹, Shuqin Huang¹, Tao Liu¹, Changming Xiao¹, Xiaoqing Tian², Ping Zhao¹, Xiaoyan Dai³, Taocui Huang^1,*, Yan Zhou^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 815-835, 2023, DOI:10.32604/phyton.2023.023928 - 29 November 2022

Abstract Gibberellin 2-oxidases (GA2ox) are important enzymes that maintain the balance of bioactive GAs in plants. GA2ox genes have been identified and characterized in many plants, but these genes were not investigated in Brassica napus. Here, we identified 31 GA2ox genes in B. napus and 15 of these BnaGA2ox genes were distributed in the A and C subgenomes. Subcellular localization predictions suggested that all BnaGA2ox proteins were localized in the cytoplasm, and gene structure analysis showed that the BnaGA2ox genes contained 2–4 exons. Phylogenetic analysis indicated that BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups, including two… More >

Ruizhi Huang¹, Huasheng Yu², Yong Yang¹, Heqin Liu², Xuelong Wu¹, Zhihong Liu¹, Haiyan He¹, Gengwei Wu¹, Wengjia Wang¹, Hua Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 333-348, 2023, DOI:10.32604/phyton.2022.023252 - 12 October 2022

Abstract As global temperature rise, the threat of heat stress to rapeseed production is becoming more obvious. Exploring the response characteristics of two important biological pathways, oil accumulation and photosynthesis, to heat stress during B. napus seed filling is helpful in the genetic improvement of heat-tolerant rapeseed. The effects of heat stress on seed oil accumulation and chlorophyll fluorescence characteristics of 29 B. napus germplasms with different oil content and environmental sensitivity, including 6 rapeseed varieties which exhibited environment-sensitive/insensitive and with high, medium or low oil content, were tested by whole plant heat stress or the in vitro silique… More >

Reza Shokri-Gharelo¹, Ali Bandehagh^1,*, Mohammad Anwar Hossain^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1403-1417, 2022, DOI:10.32604/phyton.2022.020431 - 14 March 2022

Abstract Drought stress is one of the most important abiotic stresses that plants face frequently in nature. Under drought conditions, many morphological, physiological, and molecular aspects of plants are changed and as a result plants experience a remarkable reduction in growth, yield, and reproduction. To expand our understanding of the molecular basis of the plant response to drought stress, the proteomic profile and protein-protein network of canola (Brassica napus L.) were studied. The focus was to show molecular mechanisms related to canola susceptibility to drought stress. The experiment used a completely randomized design, implemented in a hydroponic… More >

Haijun Sun^1,2, Paramsothy Jeyakumar³, Hongdong Xiao², Xuewen Li², Jiayou Liu², Min Yu², Prabal Bir Jung Rana¹, Weiming Shi^2,4,*

Phyton-International Journal of Experimental Botany, Vol.91, No.1, pp. 197-206, 2022, DOI:10.32604/phyton.2022.016492 - 16 August 2021

Abstract

There are few evidences on the effect of biochar on vegetable yield, nitrogen (N) and phosphorus (P) leaching losses under intensive vegetable production soil. The current field plot scale study evaluated responses of Chinese cabbage (Brassica oleracea L.) yield, N and P leaching losses using five N treatments of common N application rate according to local farmers’ practice (N100%), reducing 20% or 40% N fertilizer (N80% and N60%), and reducing 40% N fertilizer but incorporating 10 or 20 t/ha biochar (N60% + BC10 and N60% + BC20). Results showed that N80% and N60% decreased both the cabbage

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