Ying Wang¹, Weijun Zhang¹, Fei Cai¹, Yong Tao^2,*

Congenital Heart Disease, Vol.19, No.6, pp. 653-669, 2024, DOI:10.32604/chd.2025.060406 - 27 January 2025

Abstract Background: Previous studies combined integrated scRNA-seq with bulk RNA data to screen biomarkers for cardiomyopathy. This study extended this approach to identify biomarkers specific for hypertrophic cardiomyopathy (HCM). Methods: Datasets GSE36961, GSE130036, GSE249925 and GSE203274 were analyzed in this study. ScRNA-seq analysis was employed to identify distinct cell populations. Differentially expression analysis was conducted to screen vascular endothelial cells (VECs)-related feature genes. After calculating VECs score, WGCNA was used to correlate gene modules with the VECs score. Key HCM biomarkers were determined using random forest analysis, and LASSO regression analyses to construct a diagnostic model… More >

Domenico Crea¹, Arben Dedja¹, Matteo Ponzoni^1,2, Stefania Rizzo³, Alberto Cipriani⁴, Riccardo Bariani⁴, Kalliopi Pilichou³, Maria Bueno Marinas³, Danila Azzolina⁵, Massimo A. Padalino^1,6,*

Congenital Heart Disease, Vol.19, No.5, pp. 473-487, 2024, DOI:10.32604/chd.2025.057014 - 31 December 2024

Abstract Background: Since 2015, the pulmonary artery banding (PAB), following the Giessen protocol, has treated end-stage heart failure in selected infants with preserved right ventricular function, acting as a bridge to transplant or recovery, as a result of ventricular-ventricular interaction. Objectives: To elucidate whether PAB is a feasible and reproducible procedure in a rodent model of pharmacologically induced dilated cardiomyopathy (DCM) and to evaluate PAB-induced ventricular rehabilitation. Methods: We used 49 Sprague-Dawley rats divided into four groups: a sham surgery control group, a healthy animal group undergoing PAB, a doxorubicin (DOX)- treated control group, and a DOX… More >

CONGCHEN BAI¹, QIHANG KONG², HAO TANG², SHUWEN ZHANG², JUNTENG ZHOU^3,*, XIAOJING LIU^2,4,*

BIOCELL, Vol.47, No.11, pp. 2517-2533, 2023, DOI:10.32604/biocell.2023.043864 - 27 November 2023

Abstract Background: Various physiological mechanisms are linked to dilated cardiomyopathy (DCM) development, including oxidative stress, immune irregularities, inflammation, fibrosis, and genetic changes. However, precise molecular drivers of DCM, especially regarding abnormal immune responses, remain unclear. This study investigates immune-related long non-coding RNAs (lncRNAs) in DCM’s diagnostic and therapeutic potential. Methods: GSE141910, GSE135055, and GSE165303 datasets were acquired from the GEO database. LASSO, SVM-RFE, and random forest algorithms identified DCM-associated immune-related lncRNAs. Diagnostic capabilities were assessed by Nomogram and receiver operating characteristic (ROC) curves. Multivariate linear regression explored lncRNA correlations with ejection fraction. Single-sample gene set enrichment analysis… More > Graphic Abstract

YAO LU^1,2,*, QIUYUE WANG¹, CAIHUI ZHANG¹

BIOCELL, Vol.47, No.2, pp. 393-400, 2023, DOI:10.32604/biocell.2023.024944 - 18 November 2022

Abstract Myocardial fibrosis is an important manifestation of diabetic cardiomyopathy. This study investigated the potential mechanism of diabetic myocardial fibrosis. Male C57BL/6J and db/db mice aged 8 weeks were randomly divided into the diabetic (DB) and control groups. At 20 weeks, the mouse heart was harvested and subjected to hematoxylin-eosin staining (HE) and Masson staining to investigate the degree of fibrosis. The expressions of transforming growth factor-beta 1 (TGF-β1), collagen-III, B-cell lymphoma-2 (Bcl2), Bcl2-associated X protein (Bax), cleaved gasdermin D (GSDMD), cysteinyl aspartate specific proteinase-1 (caspase-1), apoptosis-associated speck-like protein containing a CARD (ASC), and nucleotide-binding oligomerization… More >

Junsung Park¹, Go Hun Seo², Yena Lee¹, Yunha Choi¹, Minji Kang³, Hyo-Sang Do³, Young-Hwue Kim⁴, Jeong Jin Yu⁴, Ellen Ai-Rhan Kim⁵, Euiseok Jung⁵, Byong Sop Lee⁵, Jae Suk Baek^4,#,*, Beom Hee Lee^1,6,#,*

Congenital Heart Disease, Vol.16, No.4, pp. 397-410, 2021, DOI:10.32604/CHD.2021.015167 - 19 April 2021

Abstract Background and Method: The genetic cause of infantile-onset cardiomyopathy is rarely investigated. Here, we conducted whole exome sequencing (WES) and mitochondrial DNA (mtDNA) sequencing in eight patients with infantile-onset cardiomyopathy to identify genetic variations. Result: Among these patients, two (25%) had dilated cardiomyopathy (DCMP), two (25%) had left ventricular non-compaction (LVNC), and four (50%) had hypertrophic cardiomyopathy (HCMP). Except four patients identified prenatally, the remaining patients presented at a median age of 85.5 days. WES identified genetic variants in a total of seven (87.5%) patients and mtDNA sequencing in the other case. TPM1 and MYH7 variants were identified… More >

Jacqueline S. Lee^1,2, Angelica Blais^1,2, Julia Jackson¹, Bhavika J. Patel¹, Lillian Lai⁴, Gary Goldfield^1,3, Renee Sananes⁵, Patricia E. Longmuir^1,2,3,*

Congenital Heart Disease, Vol.16, No.3, pp. 255-267, 2021, DOI:10.32604/CHD.2021.014628 - 02 March 2021

Abstract Background: Pediatric cardiology patients often experience decreased quality of life (QoL) and higher rates of mental illness, particularly with severe disease, but the relationship between them and comparisons across diagnostic groups are limited. This mixed-methods cross-sectional study assessed the association between QoL anxiety and behavior problems among children with structural heart disease, arrhythmia, or other cardiac diagnoses. Methods: Children (6–14 years, n = 76, 50% female) and their parents completed measures of QoL (PedsQL), behavior (BASC-2, subset of 19 children) and anxiety (MASC-2, children 8+ years). Pearson correlations/regression models examined associations between QoL, behavior and anxiety,… More >

Chao Luo¹, Canghong Shi¹, Xiaojie Li^{1, *}, Xin Wang⁴, Yucheng Chen³, Dongrui Gao¹, Youbing Yin⁴, Qi Song⁴, Xi Wu¹, Jiliu Zhou¹

CMC-Computers, Materials & Continua, Vol.63, No.2, pp. 995-1012, 2020, DOI:10.32604/cmc.2020.07968 - 01 May 2020

Abstract Myocardial segmentation and classification play a major role in the diagnosis of cardiovascular disease. Dilated Cardiomyopathy (DCM) is a kind of common chronic and life-threatening cardiopathy. Early diagnostics significantly increases the chances of correct treatment and survival. However, accurate and rapid diagnosis of DCM is still challenge due to high variability of cardiac structure, low contrast cardiac magnetic resonance (CMR) images, and intrinsic noise in synthetic CMR images caused by motion artifact and cardiac dynamics. Moreover, visual assessment and empirical evaluation are widely used in routine clinical diagnosis, but they are subject to high inter-observer… More >

Xueying Huang^1,*, Long Deng², Chun Yang³, Mary Lesperance⁴, Dalin Tang⁵

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 58-58, 2019, DOI:10.32604/mcb.2019.06969

Abstract Hypertrophic cardiomyopathy (HCM) occurs in about 1 of every 500 adults in the general population. It has been reported that left ventricular outflow tract obstruction (LVOTO) is observed in 70% patients with HCM. Systolic anterior motion (SAM) of the mitral valve (MV) is the dominant cause of dynamic outflow tract obstruction in most patients with hypertrophic obstructive cardiomyopathy (HOCM). Currently, the hemodynamic mechanisms of SAM remain unclear. In this study, we developed 12 active and corresponding passive models based on 6 patients’ pre- and post-operative ECG-gated cardiac CT images of patients’ LV at the pre-SAM… More >

Mohammadali Sharzehee¹, Yuan Chang², Jiang-ping Song², Hai-Chao Han^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 16-17, 2019, DOI:10.32604/mcb.2019.07129

Abstract A myocardial bridge (MB), a congenital anomaly of the coronary artery, occurs when a segment of the epicardial coronary artery goes underneath the myocardium. MBs are often observed in the middle part of the left anterior descending (LAD) artery. MB squeezes the vessel wall periodically and induces hemodynamic abnormalities which are correlated with angina and myocardial ischemia. The level of hemodynamics disturbances induced by MB depends on the myocardial bridge length, the degree of myocardial contractility, thickness, and location [1]. Hypertrophic cardiomyopathy (HCM), characterized by abnormal thickening of the heart wall, is a leading cause… More >

Katharina Niedermayr¹, Gerhard Pölzl², Sabine Scholl‐Bürgi¹, Christine Fauth³, Ulrich Schweigmann¹, Edda Haberlandt¹, Ursula Albrecht¹, Manuela Zlamy¹, Wolfgang Sperl⁴, Johannes A. Mayr⁴, Daniela Karall¹

Congenital Heart Disease, Vol.13, No.5, pp. 671-677, 2018, DOI:10.1111/chd.12634

Abstract Objective: In general, a mitochondrial disorder is diagnosed on the basis of symptom combinations and confirmed by genetic findings. However, patients carrying the m.3243A>G mutation in the mitochondrial tRNA leucine 1 (MT‐TL1) do not always meet all the proposed criteria for the most frequently encountered mitochondrial syndrome “MELAS,” an acronym for Mitochondrial Encephalomyopathy, Lactic Acidosis, and at least one Stroke‐like episode. We here present various phenotypic characteristics of the mitochondrial mutation m.3243A>G with particular focus on cardiac manifestations.
Methods and Results: We followed nine patients (1 month to 68 years old; median 42 years; four female and five male) from… More >