Yunfeng Chen1,2,3,†,*, Jiexi Liao4,†, Zhou Yuan1, Kaitao Li4, Baoyu Liu4, Lining Arnold Ju4,5,6, Cheng Zhu1,2,4,5,*
Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 97-97, 2019, DOI:10.32604/mcb.2019.07123
Abstract Force-mediated molecular binding initiates numerous cellular activities such as cell adhesion, migration, and activation. Dynamic force spectroscopy (DFS) is widely used to examine molecular binding and cell mechano-signaling [1]. The rate of dissociation, off-rate, is an important attribute of molecular binding that reflects bond stability. Extensive DFS works have demonstrated that off-rates are a function of force magnitude, yielding signature bond behaviors like “catch bond” [2]. However, as a controversial topic of the field, different DFS assays, i.e., force-clamp and force-ramp assays, often yielded distinctive "off-rate vs. force" relations from the same molecular system [3]. Such discrepancies cast doubt on… More >