ZHIPING WEI^1,2, YAJUN LIU¹, MEIWEN YANG³, MENGDI LI¹, KEXIN LI¹, LUXI ZHENG¹, HUIQIONG GUO¹, FENFANG HONG^4,*, SHULONG YANG^1,*

BIOCELL, Vol.45, No.4, pp. 1005-1012, 2021, DOI:10.32604/biocell.2021.014581

Abstract Background and Objective: LTB4 has been shown to be involved in rheumatoid arthritis (RA) pathogenesis. The effect of Dioscin(Dio) on the LTB4 pathway of RA have not been reported yet. This study aimed at further exploring whether Dioscin’s effects on TNF-α induced collagen-induced arthritis (CIA) rat fibroblast-like synoviocytes (FLS) connected with the LTB4 and its receptor pathway. Materials & Methods: In this experiment, control group, TNF-α group, and different concentrations of Dioscin groups were established. Cell viability was evaluated using MTT assay. The levels of LTB4 in the samples of above groups were measured using ELISA. The mRNA expression levels… More >

Antonio Pizzi^*

Journal of Renewable Materials, Vol.9, No.8, pp. 1345-1364, 2021, DOI:10.32604/jrm.2021.015663

Abstract Collagen powder hydrolysates were reacted with a solution of commercial mimosa bark tannin extract. The mixture was prepared at ambient temperature and prepared at 80°C to determine what reactions, if any, did occur between the collagen protein through its amino acids and the polyphenolic condensed tannin. The reaction products obtained were analyzed by matrix assisted laser desorption ionization time-of-flight (MALDI ToF) mass spectrometry. Reactions between the two materials did appear to occur, with the formation of a relatively small proportion of covalent and ionic linkages at ambient temperature but a considerable proportion of covalent linkages tannin-protein amino acids and the… More >

JIANHUA LI^1,#, YAOGANG ZHANG^1,2,#, TAO ZHANG³, MEIYUAN TIAN¹, JING HOU¹, DENGLIANG HUANG¹, YAN CHENG^1,3, ZHU MAN^1,3, XIAOMING SU^1,3, ZHIQIN LI¹, SIXIAN TONG¹, XUAN ZHANG^1,3, JUN DENG^1,3, YUN DONG^1,3, YANYAN MA^1,2,3,*

BIOCELL, Vol.44, No.2, pp. 209-215, 2020, DOI:10.32604/biocell.2020.08941

Abstract The aim of this study was to find a way to efficiently separate neuronal cells from the cerebral cortex of adult rats, providing a reference method for rapid acquisition of neuronal cells from the adult rat brain. Fifteen SD rats were randomly divided into three groups, with five SD rats in each group. Then, neuron cells were isolated from the adult rat cerebral cortex by the grinding method, the trypsin method, and the collagenase II method, respectively. The expression of anti-NeuN in the neurons of each group was analyzed by flow cytometry. The acquisition rates and morphology of neurons of… More >

Monica Puccini^*1, Eleonora Stefanelli¹, Maurizia Seggiani¹, Elena Balestri², Sandra Vitolo¹

Journal of Renewable Materials, Vol.5, Suppl.1, pp. 117-123, 2017, DOI:10.7569/JRM.2017.634138

Abstract In this study, blends of low-density polyethylene (PE) containing 20 wt% of hydrolyzed collagen (HC) from the leather industry were processed by the film blowing technique. A biodegradation study of these innovative materials was performed by two different biodegradation tests, one in terrestrial environment, the other one under aquatic conditions. Degradation rates were determined for both systems and an environmental degradability parameter was calculated. The results proved the positive influence of hydrolyzed collagen on degradation of polyethylene, but also showed a relatively low biological degradability of PE/HC blends under the applied test conditions. More >

SIMA GODOSEVICIUS KATZ

BIOCELL, Vol.29, No.3, pp. 261-270, 2005, DOI:10.32604/biocell.2005.29.261

Abstract The interaction of antimesometrial decidual cells and collagen fibrils was studied by light microscopy and ultrastructural cytochemistry in fed and acutely fasted mice on days 9-11 of pregnancy.
Fibrillar elements in the extracellular space consisted of collagen fibrils and filamentous aggregates (disintegrating collagen fibrils). Intracellular vacuoles exhibited typical collagen immersed in electron-translucent material (clear vacuoles) and faint cross-banded collagen immersed in electron-opaque material (dark vacuoles).
Fibrillar elements showed extracellular acid phosphatase activity which was stronger in the region of mature decidua than in predecidual cells region in all animals; it was conspicuous in mature decidua of fasted animals.… More >

HILDA L. MONTRULL, NILDA Y. BRIZUELA, SILVIA L. DEMURTAS, LUIS SPITALE* , CARLOS I. MEIROVICH.

BIOCELL, Vol.29, No.2, pp. 163-167, 2005, DOI:10.32604/biocell.2005.29.163

Abstract Cartilage samples were taken from OA patients in order to describe and quantify pro-inflammatory mediators. Samples were cultured under aseptic conditions in Dulbecco’s modified Eagle medium at 37°C for 10 days. Control samples, taken from non-inflammatory cartilage, were cultured under the same conditions. The levels of NO^-₂ and NO^-₃ were measured in the supernatant using a spectrophotometric assay. The activity of MMP-1 was quantified by ELISA.
The concentration of NO^-_x was 47.3 ± 4.1 µM in the OA cartilague and 10.7 ± 1.8 µM in the controls. The average MMP-1 activity was 3,650 ± 387 ng/ml in the OA… More >

TATIANA CARLA TOMIOSSO, LAURECIR GOMES, BENEDICTO DE CAMPOS VIDAL, EDSON ROSA PIMENTEL

BIOCELL, Vol.29, No.1, pp. 47-54, 2005, DOI:10.32604/biocell.2005.29.047

Abstract The composition and organization of the extracellular matrix of ostrich articular cartilage was investigated, using samples from the proximal and distal surfaces of the tarsometatarsus. For morphological analysis, sections were stained with toluidine blue and analyzed by polarized light microscopy. For biochemical analysis, extracellular matrix components were extracted with 4 M guanidinium chloride, fractionated on DEAE-Sephacel and analyzed by SDS-PAGE. Glycosaminoglycans were analyzed by electrophoresis in agarose gels. Structural analysis showed that the fibrils were arranged in different directions, especially on the distal surface. The protein and glycosaminoglycan contents of this region were higher than in the other regions. SDS-PAGE… More >

H-L. Wang¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 145-146, 2006, DOI:10.32604/mcb.2006.003.145

Abstract This article has no abstract. More >

LINGFENG CHEN^1,2,3, ZHIPENG GAO^1,2,3, BAIMEI LIU^1,2,3, YING LV^1,2,3, MEIWEN AN^1,2,3,*, JILING FENG^4,*

BIOCELL, Vol.42, No.1, pp. 25-34, 2018, DOI:10.32604/biocell.2018.06114

Abstract Arterial characterization of healthy descending thoracic aorta (DTA) is indispensable in determining stress distributions across wall thickness and different regions that may be responsible for aorta inhomogeneous dilation, rupture, and dissection when aneurysm occurs. Few studies have shown the inhomogeneity of DTA along the aorta tree considering changes in circumferential direction. The present study aims to clarify the circumferential regional characterization of DTA. Porcine DTA tissues were tested according to region and orientation using uniaxial tension. For axial test, results show that the difference in circumferential direction was mainly in collagen fiber modulus, where the anterior collagen fiber modulus was… More >

David Azria^1,2, Raluca Guermache^1,2, Sophie Raisin¹, Sébastien Blanquer¹, Frédéric Gobeaux³, Marie Morille¹, Emmanuel Belamie^1,2,*

Journal of Renewable Materials, Vol.6, No.3, pp. 314-324, 2018, DOI:10.7569/JRM.2017.634186

Abstract Biopolymers extracted from renewable resources like chitosan and collagen exhibit interesting properties for the elaboration of materials designed for tissue engineering applications, among which are their hydrophilicity, biocompatibility and biodegradability. In many cases, functional recovery of an injured tissue or organ requires oriented cell outgrowth, which is particularly critical for nerve regeneration. Therefore, there is a growing interest for the elaboration of materials exhibiting functionalization gradients able to guide cells. Here, we explore an original way of elaborating such gradients by assembling particles from a library of functionalized microspheres. We propose a simple process to prepare chitosan-collagen hybrid microspheres by… More >