GANG LIU^1,#, LEI SHI^1,#, BIN WANG¹, ZEHUI WU¹, HAIYUAN ZHAO¹, TIANYU ZHAO², LIANGHUI SHI^1,*

Oncology Research, Vol.32, No.3, pp. 585-596, 2024, DOI:10.32604/or.2023.029349

Abstract The role of lncRNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in colon cancer involves various tumorigenic processes and has been studied widely. However, the mechanism by which it promotes colon cancer remains unclear. Retroviral vector pSEB61 was retrofitted in established HCT116-siKCN and SW480-siKCN cells to silence KCNQ1OT1. Cellular proliferation was measured using CCK8 assay, and flow cytometry (FCM) detected cell cycle changes. RNA sequencing (RNA-Seq) analysis showed differentially expressed genes (DEGs). Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were carried out to analyze enriched functions and signaling pathways. RT-qPCR, immunofluorescence, and western blotting… More >

WEI HU^1,#, THOMAS WARTMANN^2,#, MARCO STRECKER², ARISTOTELIS PERRAKIS², ROLAND CRONER², ARPAD SZALLASI³, WENJIE SHI^2,*, ULF D. KAHLERT^2,*

Oncology Research, Vol.32, No.1, pp. 227-239, 2024, DOI:10.32604/or.2023.043053

Abstract Transient receptor potential (TRP) channels are strongly associated with colon cancer development and progression. This study leveraged a multivariate Cox regression model on publicly available datasets to construct a TRP channels-associated gene signature, with further validation of signature in real world samples from our hospital treated patient samples. Kaplan-Meier (K-M) survival analysis and receiver operating characteristic (ROC) curves were employed to evaluate this gene signature’s predictive accuracy and robustness in both training and testing cohorts, respectively. Additionally, the study utilized the CIBERSORT algorithm and single-sample gene set enrichment analysis to explore the signature’s immune infiltration landscape and underlying functional implications.… More > Graphic Abstract

ELYN AMIELA SALLEH¹, YEONG YEH LEE², ANDEE DZULKARNAEN ZAKARIA³, NUR ASYILLA CHE JALIL⁴, MARAHAINI MUSA^1,*

BIOCELL, Vol.47, No.10, pp. 2233-2244, 2023, DOI:10.32604/biocell.2023.030541

Abstract Colorectal cancer (CRC) is a major global health concern. Accumulation of cancer-associated fibroblasts (CAFs) in CRC is associated with poor prognosis and disease recurrence. CAFs are the main cellular component of the tumor microenvironment. CAF-tumor cell interplay, which is facilitated by various secretomes, drives colorectal carcinogenesis. The complexity of CAF populations contributes to the heterogeneity of CRC and influences patient survival and treatment response. Due to their significant roles in colorectal carcinogenesis, different clinical applications utilizing or targeting CAFs have been suggested. Circulating CAFs (cCAFs) which can be detected in blood samples, have been proposed to help in determining patient… More > Graphic Abstract

LIANLI NI^1,2,#, YUN YU^1,2,#, HAN LIN^1,2, WEISHAN ZHUGE², LU TAO², YIWEI SHEN², RI CUI^2,*, SHAOTANG LI^1,2,*

BIOCELL, Vol.47, No.10, pp. 2245-2254, 2023, DOI:10.32604/biocell.2023.030147

Abstract Background: Homeobox B8 (HOXB8), a member of HOX family, plays a key role in the development of colorectal cancer (CRC). However, the function of HOXB8 in oxaliplatin (OXA) resistance in CRC is still unclear. This study investigated the role and precise molecular mechanism of HOXB8 in OXA-resistant CRC cells. Methods: The cell viability was measured by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, and the colony forming ability was determined by colony formation assay. The silencing RNA (siRNA) approach was used to knockdown HOXB8 in CRC cells while the lentiviral transfection system was used to establish stable HOXB8 overexpressing CRC… More >

Ushaa Eswaran^1,*, S. Anand²

Computer Systems Science and Engineering, Vol.46, No.2, pp. 1917-1928, 2023, DOI:10.32604/csse.2023.032523

Abstract Colon cancer is the third most commonly diagnosed cancer in the world. Most colon AdenoCArcinoma (ACA) arises from pre-existing benign polyps in the mucosa of the bowel. Thus, detecting benign at the earliest helps reduce the mortality rate. In this work, a Predictive Modeling System (PMS) is developed for the classification of colon cancer using the Horizontal Voting Ensemble (HVE) method. Identifying different patterns in microscopic images is essential to an effective classification system. A twelve-layer deep learning architecture has been developed to extract these patterns. The developed HVE algorithm can increase the system’s performance according to the combined models… More >

HUAMIN WANG^1,#, YANTING WU^1,#, ZHENLEI WANG^2,#, YUHANG CHEN¹, JINYU MO¹, WEN GUAN¹, YALI ZHANG¹, HONGLIANG YAO^1,*

Oncology Research, Vol.29, No.3, pp. 201-215, 2021, DOI:10.32604/or.2022.03553

Abstract LncRNAs and metabolism represents two factors involved in cancer initiation and progression. However, the interaction between lncRNAs and metabolism remains to be fully explored. In this study, lncRNA FEZF1-AS1 (FEZF1- AS1) was found upregulated in colon cancer after screening all the lncRNAs of colon cancer tissues deposited in TCGA, the result of which was further confirmed by RNAscope staining on a colon tissue chip. The results obtained using FEZF1- AS1 knockout colon cancer cells (SW480 KO and HCT-116 KO) constructed using CRISPR/Cas9 system confirmed the proliferation, invasion, and migration-promoting function of FEZF1-AS1 in vitro. Mechanistically, FEZF1-AS1 associated with the mitochondrial… More >

Juan Gu^*, Chang-fu Cui^†, Li Yang^‡, Ling Wang^*, Xue-hua Jiang^*

Oncology Research, Vol.27, No.2, pp. 193-202, 2019, DOI:10.3727/096504018X15150662230295

Abstract Colon cancer (CC) is the third most common cancer worldwide. Emodin is an anthraquinone-active substance that has the ability to affect tumor progression. Our study aims to explore the effects and the relevant mechanism of emodin on the invasion and migration of CC in vitro and in vivo. In our study, we found that emodin inhibited the invasion and migration abilities of RKO cells and decreased the expression of matrix metalloproteinase-7 (MMP-7), MMP-9, and vascular endothelial growth factor (VEGF) in a dose-dependent manner. Further research suggested that emodin inhibited EMT by increasing the mRNA level of E-cadherin and decreasing the… More >

Xiaofei Ning^*, Cong Wang^†, Meng Zhang^†, Kecheng Wang^*

Oncology Research, Vol.27, No.4, pp. 399-406, 2019, DOI:10.3727/096504018X15179675206495

Abstract Colon cancer is one of the most common cancers in the world. Epithelial-to-mesenchymal transition (EMT) is a crucial step in tumor progression and is also involved in the acquisition of stem cell-like properties. Some miRNAs have been shown to function as either tumor suppressors or oncogenes in colon cancer. Here we investigated the role of miR-147 in the regulation of the stem cell-like traits of colon cancer cells. We observed that miR-147 was downregulated in several colon cancer cell lines, and overexpressed miR-147 decreased the expression of cancer stem cell (CSC) markers OCT4, SOX2, and NANOG in the colon cancer… More >

Fen Liu^*†, Shaojun Liu^*, Feiyan Ai^*†, Decai Zhang^*†, Zhiming Xiao^*, Xinmin Nie^‡, Yunfeng Fu^§

Oncology Research, Vol.28, No.5, pp. 553-557, 2020, DOI:10.3727/096504020X16032056440094

Abstract Colorectal cancer (CRC) is one of the most common malignancies in the world, with a high incidence and a high mortality. However, the pathogenesis of CRC carcinogenesis is still unexplored. In this study, we investigated the role of miR-107 in the regulation of CRC cell proliferation and apoptosis. First, the expression of miR-107 was observed to be aberrantly increased in human CRC tumor tissues and cell lines when compared to the colonic control tissues and colon epithelial cells. Further study showed that the proliferative and apoptotic capacities of human CRC SW480 and LoVo cells were aberrantly regulated by miR-107. The… More >

Jing Zeng^*1, Tianping Du^†1, Yafeng Song^‡, Yan Gao^‡, Fuyan Li^‡, Ruimin Wu^‡, Yijia Chen^‡, Wei Li^‡, Hong Zhou^‡, Yi Yang^‡, Zhijun Pei^‡

Oncology Research, Vol.28, No.5, pp. 551-552, 2020, DOI:10.3727/096504020X16032056440085

Abstract Long noncoding RNA (lncRNA) colon cancer-associated transcript 2 (CCAT2) has been demonstrated to play an important role in diverse tumorigenesis. However, the biological function of lncRNAs in glioma is still unknown. In this study, we found that lncRNA CCAT2 was overexpressed in glioma tissues and cell lines and associated with tumor grade and size. Furthermore, patients with high levels of lncRNA CCAT2 had poorer survival than those with lower levels of lncRNA CCAT2. Knocking down lncRNA CCAT2 expression significantly suppressed the glioma cell growth, migration, and invasion, as well as induced early apoptosis of glioma cells in vitro. Moreover, lncRNA… More >