Suzana Vieira Rabêlo¹, Fernanda Granja da Silva Oliveira², Michele Miriam Calixto de Lira³, Livia Macedo Dutra³, Adilson Sartoratto⁴, Marta Cristina Teixeira Duarte⁴, Maria Claudia dos Santos Luciano⁵, Maria Francilene Souza Silva⁵, Cláudia do Ó Pessoa⁵, Manoel Odorico de Moraes Filho⁵, Emmanoel Vilaça Costa⁶, Jackson Roberto Guedes da Silva Almeida^1,3,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 921-931, 2021, DOI:10.32604/phyton.2021.013305

Abstract This study aimed to identify the chemical composition of essential oil from fruits (EOAF) and the hexanic crude extract from aerial parts (At-Hex) of atemoya (Annona cherimola x Annona squamosa), a hybrid belonging to the Annonaceae family. Cytotoxic and antimicrobial activity was also evaluated. OEAF was obtained by hydrodistillation using a Clevenger apparatus, and their composition was analyzed by gas chromatography-mass spectrometry (GC-MS) analyses. Cytotoxicity was tested against human tumor cell lines HCT-116 (colon carcinoma), SF-295 (glioblastoma), OVCAR-8 (ovarian carcinoma) and HL60 (leukemia) using 3-(4,5-dimethyl-2-thiazolyl)-2,5- diphenyl-2H-tetrazolium bromide (MTT) assay, while antimicrobial activity was conducted by bioauthography method against eleven microorganisms… More >

SINEM GÖKÇE KÜTÜK¹, MUSTAFA NAZIROĞLU^2,3,*

BIOCELL, Vol.45, No.1, pp. 177-188, 2021, DOI:10.32604/biocell.2021.013531

Abstract A protective action of melatonin (MELAT) on docetaxel (DCT)-induced inflammation, apoptosis, and reactive free oxygen radical (fROS) generation values via blocking of TRPM2 calcium-permeable channel was investigated in different cells except for laryngo-tracheal epithelial (LT-Epi) cells. Hence, the protective action of MELAT on DCT-induced oxidative toxicity and inflammation in LT-Epi tissue and cells of mice were investigated in the current study. MELAT treatment ameliorated DCTinduced mitochondrial ROS in the LT-Epi cells by reducing the generation of fROS (cytosolic and mitochondrial), lipid peroxidation, and depolarization of the mitochondrial membrane, while increasing reduced glutathione (GSH), GSH peroxidase, and total antioxidant status. In… More >

LI-NA XU^1#, BI-NAN LU^2#, MING-MING HU¹, YOU-WEI XU¹, XU HAN¹, YAN QI¹, JIN-YONG PENG^1,3*

BIOCELL, Vol.36, No.3, pp. 113-120, 2012, DOI:10.32604/biocell.2012.36.113

Abstract Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose- and time-dependent manner. Morphology of apoptotic cells was studied with acridine orange/ ethidium bromide staining. The concentrations of lactate… More >

ANURADHA SEHRAWAT AND VIJAY KUMAR^*

BIOCELL, Vol.36, No.2, pp. 63-71, 2012, DOI:10.32604/biocell.2012.36.063

Abstract The flower of Butea monosperma (Lam.) (Fabaceae) has been used in traditional Indian medicine in the treatment of many ailments including liver disorders. To understand the pharmacological basis of its beneficial effects, the extracts of dried flowers in water, methanol, butanol, ethyl acetate and acetone were evaluated for free radical scavenging and pro-apoptotic activities in cell cultures (human hepatoma Huh-7 cell line and immortalized AML-12 mouse hepatocytes). Butrin and butein -the active constituents of flower extracts- were used as reference molecules. The levels of cell injury markers like lactate dehydrogenase, glutathione and lipid peroxidation and primary antioxidant enzymes glutathione S-transferase… More >

VIJAY L. KUMAR*, ABHISHEK SINGHAL

BIOCELL, Vol.33, No.1, pp. 19-24, 2009, DOI:10.32604/biocell.2009.33.019

Abstract Cytotoxic properties of plant extracts and drugs being developed for cancer treatment are usually evaluated by a variety of in vivo and in vitro tests carried out in animal or plant based models. In the present study we have evaluated the possibility of using the germinating mung beans (Vigna radiata), for rapid and inexpensive screening of drugs exhibiting cytotoxic properties. Mung beans were allowed to germinate either in tap water or in different drug solutions, and parameters like percent germination, increase in radicle length, change in seedling weight and mitotic index of apical root meristems were determined at two time… More >

R. SEHGAL, S. ROY, V.L. KUMAR*

BIOCELL, Vol.30, No.1, pp. 9-13, 2006, DOI:10.32604/biocell.2006.30.009

Abstract In the present study we have utilized the Allium cepa root tip meristem model to evaluate the cytotoxic and anti-mitotic activities of latex of Calotropis procera (DL) and podophyllotoxin. Standard cytotoxic drug cyclophosphamide and non-cytotoxic drugs cyproheptadine and aspirin served as controls. Like cyclophosphamide, both DL and podophyllotoxin significantly inhibited the growth of roots and mitotic activity in a dose-dependent manner. However, podophyllotoxin was more potent in this regard and produced root decay. Cyproheptadine and aspirin, on the other hand, showed a marginal effect on the root growth and mitotic activity at much higher concentrations. More >

Mohammad Reza RAMEZANI¹, Hossein NADERI-MANESH^{1, *}, Hossain-Ali RAFIEEPOUR²

BIOCELL, Vol.38, No.1, pp. 11-16, 2014, DOI:10.32604/biocell.2014.38.011

Abstract Cell-based biosensors have become a research hotspot in the biosensors and bioelectronics fields. The main feature of cell-based biosensors is immobilization of living cells on the surface of transducers. Different types of polymers which are used as scaffolds for cell growth should be biocompatible and should have reactive functional groups for further attachment of biomolecules. In this work, cell attachment and proliferation on chitosan hydrogel, chitosan-gelatin and gold nanoparticle-chitosan nanocomposite membranes was studied. Characterization of the membranes was performed using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Cytotoxicity assessment on HEK293 cells was carried out for all… More >

Orlando J. CASTEJÓN^*

BIOCELL, Vol.39, No.2-3, pp. 33-40, 2015, DOI:10.32604/biocell.2015.39.033

Abstract Twenty one days old Swiss albino mice that received diphenylhydantoin (25 mg/kg, i.p., daily for 15 days) progressively developed gait alterations, changes of behavior and cerebellar ataxia. Cerebellar slices were processed by conventional transmission electron microscopy. The body of Purkinje cells exhibited fragmented limiting plasma membranes, dilated nuclear envelopes, swelling and disassembly of nuclear pores, enlargement of rough and smooth endoplasmic reticulum and a notable detachment of membrane associated ribosomes, together with distorted vacuoles of smooth endoplasmic reticulum, bizarre shaped and swollen mitochondria with dilated cristae, as well as disrupted limiting lysosomal membranes. Degenerated axosomatic synapses apparently corresponding to basket… More >

MAHMOUD MOUSTAFA^1,2*, TAREK TAHA³, MOHAMED ELNOUBY⁴, NEHAL EL-DEEB⁵, GAMAL HAMAD⁶, M.A. ABUSAIED⁷, SULAIMAN ALRUMMAN¹

BIOCELL, Vol.41, No.2-3, pp. 67-73, 2017, DOI:10.32604/biocell.2017.41.067

Abstract Current investigation has shown that human exposure to aflatoxins is not limited to the administration of contaminated cereals, but water is another possible source. This study was aimed to design easily applicable method to eliminate aflatoxin B2 (AFB2) from contaminated drinking water. Electrospinning has been used for preparation of probiotic-coated polyvinyl alcohol (PVA) and cellulose acetate (CA) nanofibers. Both of these hybrid nanofibers were studied by scanning electron microscopy (SEM) and Fourier-transformed infrared spectroscopy (FT-IR). SEM showed the proper coating of probiotic strains (Kluyveromyces lactis CBS 2359 and Saccharomyces cerevisiae ATCC 9763) on both nanofiber types. Different areas (1-5 cm²)… More >