López-España RG¹, S Hernández-Verdugo¹, S Parra-Terraza¹, F Porras¹, Antonio Pacheco-Olvera¹, A Valdez-Ortiz², T Osuna-Enciso³, MD Muy-Rangel³

Phyton-International Journal of Experimental Botany, Vol.85, pp. 131-141, 2016, DOI:10.32604/phyton.2016.85.131

Abstract Wild pepper (Capsicum annuum L. var. glabriusculum) is a valuable genetic resource for food and agriculture. The patterns of variation of 12 morphological traits of 17 populations of wild pepper collected across a latitudinal and climate gradients in northwestern Mexico were analyzed in a uniform greenhouse experiment. The morphological traits data were axamined by univariate and multivarite analysis. The relationship between phenotypic variation and latitude of population origen was tested using linear regression analysis. We found high variation within and among populations for most of the measured traits. Populations were clearly differentiated by univariate analysis of variance and principal component… More >

Liu XL^1,2, JH Li^2,3, JY Zhu², YF Yang¹

Phyton-International Journal of Experimental Botany, Vol.85, pp. 297-304, 2016, DOI:10.32604/phyton.2016.85.297

Abstract Spring ephemeroid plants complete their aboveground reproduction and growth during the short growing season, and may go dormant subsequently underground. Little is known about the underground dormancy and biological activities of the plants. In this study, we observed organogenesis and growth rhythm of rhizome buds of Adonis amurensis Regel et Radde, a spring ephemeroid plant from the Changbai Mountains in northeastern China. Our results showed that A. amurensis did not go through summer dormancy, but started producing mixed buds on rhizomes soon after the aboveground parts had died. The buds grew in length and diameter following an exponential model with… More >

Danyang Yue¹, Yijuan Fan¹, Juan Lu¹, Mengxue Zhang¹, Jin Zhou¹, Yuying Bai¹, Jun Pan^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 144-144, 2019, DOI:10.32604/mcb.2019.07084

Abstract Mesenchymal Stem Cells (MSCs) are recruited to the musculoskeletal system following trauma [1] or chemicals stimulation [2]. The regulation of their differentiation into either bone or cartilage cells is a key question. The fluid shear stress (FSS) is of pivotal importance to the development, function and even the repair of all tissues in the musculoskeletal system [3]. We previously found that MSCs are sensitive enough to distinguish a slight change of FSS stimulation during their differentiation commitment to bone or cartilage cells, and the internal mechanisms. In detail, MSCs were exposed to laminar FSS linearly increased from 0 to 10… More >

Yuhsuan Wang^1,2, Yuwei Guo^1,2, Lisha Zheng^1,2,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 99-99, 2019, DOI:10.32604/mcb.2019.06995

Abstract Many studies have shown that cell shape effects cell chromatin aggregation, gene expression, protein synthesis, cell growth, apoptosis, and cytoskeletal rearrangement [1, 2]. Dental pulp stem cells (DPSCs) are capable of osteogenic, dentinogenic, chondrogenic, and neurogenic differentiation. They are regarded as a promising candidate for tissue regeneration. How the cell shape regulates their cell behavior is still unknown. We used micropatterning technology to design single cell patterns in a 1:1, 1:2, 1:4, 1:8, 1:16 length-width ratio of rectangles with the same area. The results indicated that cell shape rearranged the cytoskeleton of DPSCs. The nuclear shape also affected by different… More >

Lili Dong¹, Yang Song^1,*, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 78-78, 2019, DOI:10.32604/mcb.2019.07130

Abstract It has been widely recognized that stem cells possess the potential of osteogenic differentiation, which greatly contribute to bone repair. Recently, accumulating evidences have indicated that mechanical cues are required for bone repair ^[1,2]. However, how local and recruited stem cells in the bone architecture receive the mechanical signals is poorly understood ^[3,4]. The purpose of this study is to demonstrate that macrophages potentially transduce the mechanical signals for stem cell osteogenic lineage. This demonstration has been carried out through a co-culture system to investigate the effect of macrophages which subjected to cyclic stretch on the osteogenic potential of bone… More >

Yu Gao¹, Meiyue Wang¹, Yanting He¹, Lanlan Li¹, Xiaodong Cui¹, Min Cheng^1,*, Xiaoyun Zhang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.1, pp. 93-93, 2019, DOI:10.32604/mcb.2019.05758

Abstract This study examines the effects of P53 in transdifferentiation of endothelial progenitor cells (EPCs) into smooth muscle cells induced by oscillatory shear stress. Endothelial progenitor cells (EPCs) were planted on slide and treated with 4 dyne/cm2 oscillatory shear stress (OSS). Results showed that the expression P53 was decreased time dependent after OSS. The OSS also attenuated the endothelial cells marker vWF and CD31 expression but enhanced the marker of smooth muscle cell α-SMA and SM22 expression in EPCs. After EPCs were pretreated with P53 agonist, the changes of angiogenesis in vitro were detected by matrix gel, and the expressions of… More >

Xiumei Guan¹, Hong Li¹, Xin Li¹, Xiaoyun Zhang¹, Xiaodong Cui¹, Hong Yan¹, Yuzhen Wang², Shunmei Liu², Min Cheng^3,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.1, pp. 91-91, 2019, DOI:10.32604/mcb.2019.05755

Abstract Aims: Endothelial progenitor cells (EPCs) play an important role in postnatal angiogenesis and neovascularization. Previous studies have revealed shear stress could accelerate EPC proliferation, differentiation, migration and so on, which contribute to postnatal angiogenesis and neovascularization. Moreover, some studies indicate that autophagy actively participates angiogenesis by affecting EPC migration and differentiation. Here, we try to elucidate the possible roles of autophagy of EPC differentiation induced by shear stress. Methods and Results: EPCs were exposed to shear stress (12 dyne/cm²). And then the expression of autophagy markers, such as LC3Ⅱ/Ⅰ, P62andATG5, were analyzed using Western blot. The results have shown that… More >

Jifeng Li^1,#, Yanting He^1,#, Xiaoyun Zhang¹, Hong Li¹, Xiumei Guan¹, Min Cheng^1,*, Xiaodong Cui^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.1, pp. 89-89, 2019, DOI:10.32604/mcb.2019.05753

Abstract Objective: The propose of the study is to investigate the specific effects of the mechanically sensitive channel Kir2.1 on the transdifferentiation of EPCs so as to understand the molecular mechanism of pathological vascular remodeling. Methods: Endothelial progenitor cells (EPCs) were isolated from rat bone marrow and cultured in EGM2 medium in vitro. The recombinant lentiviral vectors carrying Kir2.1 (NM_017296.1) gene was designed and constructed in order to overexpress the gene. The smooth muscle cells (SMCs) molecules marker on EPCs, such as α-SMA, FSP1 and α-SM22, were detected by RT-PCR and cellular immunofluorescence. In addition, cell angiogenic capacity and migration in… More >

VANINA L. JULIANELLI¹ , LILIANA N. GUERRA^*1, JUAN C. CALVO^*1,2

BIOCELL, Vol.31, No.2, pp. 237-245, 2007, DOI:10.32604/biocell.2007.31.237

Abstract Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation… More >

García-Zambrano EA¹, MC Ojeda-Zacarías¹, JG Gutiérrez-Soto¹, SR Sinagawa-García¹, A Gutiérrez-Díez¹, J Martínez-de la Cerda¹, JA Pedroza-Flores¹, C Gallegos-Vázquez², JI García-Zambrano³

Phyton-International Journal of Experimental Botany, Vol.87, pp. 87-93, 2018, DOI:10.32604/phyton.2018.87.087

Abstract The technologies based on molecular markers of DNA are the most advanced and possibly the most effective and accurate means to identify genetic variation. The objective of this study was to use molecular genetic markers namely AFLP as a tool for the molecular differentiation of 36 accessions of xoconostle collected in the Centro Regional Universitario Centro Norte of the Universidad Autónoma de Chapingo (CRUCEN-UACh.). DNA extraction was performed by the method of cellular ruptor using FastDNA^® Kit, and quantification of purity and yield of DNA was carried out through the Nanodrop 2000. It was observed that the DNA concentrations ranged… More >