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  • Open Access

    ARTICLE

    Differentiation of Fungal Destructive Behaviour of Wood by the White-Rot Fungus Fomes fomentarius by MALDI-TOF Mass Spectrometry

    Ehsan Bari1,*, Antonio Pizzi2,*, Olaf Schmidt3, Siham Amirou2, Mohammad Ali Tajick-Ghanbary4, Miha Humar5

    Journal of Renewable Materials, Vol.9, No.3, pp. 381-397, 2021, DOI:10.32604/jrm.2021.015288

    Abstract There are many methods to identify and recognize the molecular and behavioural differences between organisms. One of the methods for the detection and identification of unknown organisms as well as intermolecular and intramolecular structural differences is MALDI-TOF mass spectrometry. Therefore, differentiation of Fomes fomentarius decay capabilities on the chemical properties of the wood cell wall of the tree species Quercus castaneifolia, Juglans regia, and Carpinus betulus were used to determine and characterize the destructive behaviour of F. fomentarius decay by MALDI-TOF mass spectrometry. The results showed that the fungus had more signifi- cant destructive behaviour on J. regia than the… More >

  • Open Access

    REVIEW

    New models of adipogenic differentiation highlight a cell-autonomous response to temperature

    KSENIJA VELICKOVIC1,2,#, HILDA ANAID LUGO LEIJA1,3,#, SARAH MCGINLAY1,3,#, MICHAEL E. SYMONDS1,3, VIRGINIE SOTTILE1,4,*

    BIOCELL, Vol.44, No.4, pp. 501-512, 2020, DOI:10.32604/biocell.2020.012942

    Abstract Temperature is a key regulator of brown adipose tissue (BAT) function, acting through central sensory inputs to influence metabolism and energy storage. Although animal models have produced a wealth of information on the pathways, effectors and responses mediating the physiological response of adipose tissue to temperature in vivo, the use of cell culture models now offers evidence of an additional cell-autonomous response to temperature changes, in the absence of neural input. In particular, stem cell models provide new insight into the regulation of adipogenic differentiation and the induction of browning features in vitro. Here the basis for adipogenic responsiveness to… More >

  • Open Access

    ARTICLE

    MiR-4262 regulates differentiation and osteogenesis of human periodontal stem cells by targeting suppressor of cytokine signaling 4

    ZIMING WEI1,2, YARONG WANG3, MINGYUAN DU1,4, MINJIE LI2, HONG HE1,4,*

    BIOCELL, Vol.44, No.4, pp. 631-638, 2020, DOI:10.32604/biocell.2020.010596

    Abstract Periodontitis, as a chronic inflammatory disease, remains unsolved, and the pathogenesis of this disease has not been fully elucidated. In this study, the effect of miR-4262 was investigated in tumor necrosis factor-α (TNF-α) induced human periodontal stem cells (hPDLSCs) for the first time. The gene expression involved in this study was determined using polymerase chain reaction (PCR), the expressions of relevant proteins were determined by western blot analysis, and the levels of IL-1β, IL-6, and MCP-1 were estimated by enzyme-linked immunosorbent assay (ELISA) assay. The luciferase reporter assay was performed for verification of the target gene, alkaline phosphatase (ALP) activity… More >

  • Open Access

    ARTICLE

    Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid

    YUNZE XUAN1,#, BIN JIN1,#, SAYAN DEB DUTTA5,#, MENGMENG LIU2, ZAIXIAN SHEN1, XIWEN LIU3, YANG KANGJUAN4,*, LIM KI-TAEK5,*

    BIOCELL, Vol.44, No.3, pp. 389-400, 2020, DOI:10.32604/biocell.2020.09170

    Abstract Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth, leading to the destruction of tooth-supporting tissues and loss of alveolar bone. Suberoylanilide hydroxamic acid (SAHA), a class of histone deacetylase (HDAC) inhibitor, has the potential to stimulate osteoblast differentiation by acetylating histone proteins, and thus suppressing the expression of adipogenic and chondrogenic genes. However, the effect of SAHA on the differentiation of human periodontal ligament stem cells (hPDLSCs) is yet to be elucidated. Herein, we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs… More >

  • Open Access

    ABSTRACT

    Supplement. 10 Symposium: BIOLOGY AND CULTURE OF SILVERSIDES (PEJERREYES) Characterization of the Brain-pituitary axis in pejerrey Odontesthes bonariensis

    G.M. SOMOZA, L.A. MIRANDA, L.G. GUILGUR, P.H. STROBL-MAZZULLA

    BIOCELL, Vol.30, Suppl.S, pp. 89-95, 2006

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    Isolation of stem cells from adult rat kidneys

    YOUSOF GHEISARI1,2, MASOUD SOLEIMANI3*, SIROUS ZEINALI2, EHSAN AREFIAN1, AMIR ATASHI1,3, MAHIN NIKOUGOFTAR ZARIF4

    BIOCELL, Vol.33, No.1, pp. 33-38, 2009, DOI:10.32604/biocell.2009.33.033

    Abstract The kidney has an inherent ability for recovery and regeneration following acute damage. However, there has been much contention as to the source of regenerating renal cells. The aim of this study was to isolate and characterize these cells. Normal rat kidneys were minced and cells were isolated with collagenase I and were cultured in an expansion medium. Adherent cells were isolated and expanded for more than 120 days in vitro. These cells had the potential of trans-lineage differentiation into neural cells, adipocytes and osteocytes. These cells also expressed Nucleostemin, Cyclin D1, Notch1 and Survivin which are commonly expressed in… More >

  • Open Access

    ARTICLE

    Sequential expressions of Notch1, Jagged2 and Math1 in molar tooth germ of mouse

    SILVIA S. BORKOSKY1,2, HITOSHI NAGATSUKA1, YORIHISA ORITA3, HIDETSUGU TSUJIGIWA4, JUNKO YOSHINOBU3, MEHMET GUNDUZ1, ANDREA P. RODRIGUEZ1, LILIANA R. MISSANA2, KAZUNORI NISHIZAKI3, NORIYUKI NAGAI1

    BIOCELL, Vol.32, No.3, pp. 251-257, 2008, DOI:10.32604/biocell.2008.32.251

    Abstract The Notch signaling pathway is an evolutionary conserved mechanism that plays an important role in cell-cell communication and cell fate in a wide range of tissues. The mammalian family of Notch receptors consists of 4 members: Notch1/2/3/4. The Notch ligand family consists of 5 members: Delta1/3/4 and Jagged1/2. Math1 encodes a murine basic helix-loop-helix (bHLH) transcription factor that acts as positive regulator of cell differentiation. Recently, links between Notch and Math1 pathways were demonstrated in various tissues. Expression of Notch1, Jagged2 and Math1 were analyzed in the mouse molar tooth germ during embryonic stage (E) 13 and E15 and during… More >

  • Open Access

    ARTICLE

    Behavior of mesenchymal stem cells stained with 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) in osteogenic and non osteogenic cultures

    N.M. OCARINO*, A. BOZZI**, R.D.O. PEREIRA*, N.M. BREYNER**, V.L. SILVA*, P. CASTANHEIRA**, A.M. GOES**, R. SERAKIDES*

    BIOCELL, Vol.32, No.2, pp. 175-183, 2008, DOI:10.32604/biocell.2008.32.175

    Abstract 4’, 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long extensions that apparently linked them… More >

  • Open Access

    ARTICLE

    Differentiation and morphogenesis of Triatoma infestans (Klug 1834) female gonads. I – Post embryonic development

    CECILIA I. IBAÑEZ DE BARRETT, JUAN PABLO BOZZINI, MARTA MARIANO DE BOZZINI*

    BIOCELL, Vol.28, No.3, pp. 259-269, 2004, DOI:10.32604/biocell.2004.28.259

    Abstract The post-embryonic development of the female gonads in Triatoma infestans (Hemiptera, Heteroptera), insects of importance in health affairs as harbors and vectors of different tripanosomatidea flagellates, is presented in a complete follow-up since insect hatches from the egg up to the last molt in the fifth instar stage. The detailed description of the morphological changes which occur in each instar as well as careful measurements evaluating its size increase have been analyzed by stereomicroscopy, phase contrast, dark field, and oblique illumination, in order to optimize the observations as well the photographic register of gonad morphology and structure.
    The analysis… More >

  • Open Access

    ARTICLE

    A unique mechanism of nuclear division in Giardia lamblia involves components of the ventral disk and the nuclear envelope

    ALBERTO J. SOLARI*, MONICA I. RAHN*, ALICIA SAURA**, HUGO D. LUJAN**

    BIOCELL, Vol.27, No.3, pp. 329-346, 2003, DOI:10.32604/biocell.2003.27.329

    Abstract The fine structure of the binucleate, parasitic protist Giardia lamblia during interphase and divisional stages was studied by serial thin sectioning and three-dimensional reconstructions. The earlier sign of nuclear division is the development of a few peripheral areas of densely packed chromatin directly attached to the inner nuclear envelope. An intracytoplasmic sheet of ventral disk components grows from the cell periphery towards one of the nuclei, apparently constricting this nucleus, which becomes located at a ventral bulge. After the basal bodies become duplicated, a full nuclear division occurs in trophozoites, giving two pairs of parent-daughter nuclei. This full division occurs… More >

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