DANPING ZHU^1,#, GUANGMING LIU^1,#, KUAN FENG¹, SUYUN LI¹, DANDAN HU², SIDA YANG^3,*, PEIQING LI^1,*

BIOCELL, Vol.48, No.4, pp. 653-664, 2024, DOI:10.32604/biocell.2024.044856

Abstract Background: During Enterovirus type 71 (EV71) infection, the structural viral protein 1 (VP1) activates endoplasmic reticulum (ER) stress associated with peripheral myelin protein 22 (PMP22) accumulation and induces autophagy. However, the specific mechanism behind this process remains elusive. Methods: In this research, we used the VP1-overexpressing mouse Schwann cells (SCs) models co-transfected with a PMP22 silencing or Autocrine motility factor receptor (AMFR/gp78) overexpressing vector to explore the regulation of gp78 on PMP22 and its relationship with autophagy and apoptosis. Results: The activity of gp78 could be influenced by EV71-VP1, leading to a decrease in the ubiquitination and degradation of PMP22,… More >

Tauqeer Ahmad Yasir^1,2, Muhammad Ateeq^1,3, Allah Wasaya^1,2,*, Mubshar Hussain², Naeem Sarwar², Khuram Mubeen⁴, Mudassir Aziz⁴, Muhammad Aamir Iqbal⁵, Chukwuma C. Ogbaga⁶, Ibrahim Al-Ashkar⁷, Md Atikur Rahman⁸, Ayman El Sabagh^9,10,*

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3113-3131, 2023, DOI:10.32604/phyton.2023.029502

Abstract Drought is one of the critical limitations to agricultural soils and crop plants. Scarcity of water is increasing due to climate change that lead to increasing threats to global food security. Therefore, ecofriendly and cost effective strategies are highly desirable for mitigating drought stress along with sustainable and smart agricultural production. The aim of the study was to mitigate DS using seed priming and exogenous supplementation of β-aminobutyric acid (BABA) in linseed (Linum usitatissimum L.). Different doses (0, 50, 100 and 150 µM) of BABA were used for seed priming agent and foliar spraying under three soil moisture levels viz.,… More >

Yanlei Xu, Xue Cong, Yuting Zhai, Zhiyuan Gao, Helong Yu^*

Intelligent Automation & Soft Computing, Vol.37, No.3, pp. 3019-3037, 2023, DOI:10.32604/iasc.2023.040330

Abstract To solve inefficient water stress classification of spinach seedlings under complex background, this study proposed an automatic classification method for the water stress level of spinach seedlings based on the N-MobileNetXt (NCAM+MobileNetXt) network. Firstly, this study reconstructed the Sandglass Block to effectively increase the model accuracy; secondly, this study introduced the group convolution module and a two-dimensional adaptive average pool, which can significantly compress the model parameters and enhance the model robustness separately; finally, this study innovatively proposed the Normalization-based Channel Attention Module (NCAM) to enhance the image features obviously. The experimental results showed that the classification accuracy of N-MobileNetXt… More >

Alfaro-Corres Arnoldo Enrique^1,#, Lecona-Guzman Carlos Alberto^1,#, Gutiérrez-Miceli Federico Antonio¹, Martínez-Esteves Manuel², Silverio-Gómez María del Carmen³, Medina-Lara María de Fátima², Ruiz-Lau Nancy^4,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 629-643, 2023, DOI:10.32604/phyton.2022.023970

Abstract The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana L. In order to do the establishment of cells, the formation of callus was induced; for it two auxins were evaluated: 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-mino-3,5,6-trichloropicolinic acid (picloram) at three concentrations (0.25, 0.5 and 0.75 mg L⁻¹) in three explants (leaf, root and meristems) cultured in MS semisolid medium. The callogenesis response was related to the type and section of the explant, as well as the regulator used, and a cell suspension was established using 0.5… More >

XINTING ZHU^1,2,#, MENG YE^2,3,#, KELAN FANG¹, FANG LIU^1,2, JING HUI^1,2, MEICHEN LIU², XIAOFEI LI^1,2, RONG YAN^1,2,*, Yun Liu^1,2,4,*

BIOCELL, Vol.46, No.12, pp. 2595-2600, 2022, DOI:10.32604/biocell.2022.025468

Abstract Cantharidin (CTD) is a bioactive ingredient isolated from Cantharis vesicatoria (blister beetles), which has potential therapeutic value as an anticancer agent. Magnesium Demethylcantharidate (MDC) is a recently developed derivative of Cantharidin (CTD), and previous studies have illustrated its excellent anticancer activity on HCC cells. However, the effect and mechanism of MDC remains unclear and need to be further studied. In particular, whether MDC can cause ER stress in HCC is still unknown. In this study, we demonstrated that endoplasmic reticulum stress (ERS)-related proteins were changed in SMMC-7721 and Bel-7402 cells after being exposed to MDC. Moreover, we found that MDC… More >

Xiaotong Wei^1,#, Xuhong Fan^2,#, Xuan Lu¹, Liyuan Han¹, Honglin Zhang³, Siyan Liu³, Jing Qu³, Shuyan Guan^3,*, Yiyong Ma^3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.9, pp. 1945-1957, 2022, DOI:10.32604/phyton.2022.020062

Abstract

The embryos of maize (Zea mays L.) inbred lines GS02, GS07, GS08, GS11 and GS15 were used as receptor materials to optimize the receptor system from the aspects of genotype, medium components and stress (PEG6000, mannitol, salt and low phosphorus). The results showed that GS07 had the highest induction rate (95.2%). Orthogonal test analysis showed that the best combination of medium components in induction was A₂B₃C₁D₃₍₂₎, namely, the concentration of 2, 4-dichlorophenoxy acetic acid (2,4-D) was 4 mg·mL^-1, the concentration of L-Proline (L-Pro) was 0.8 mg·mL^-1, and the concentration of silver nitrate (AgNO₃) was 10 mg·mL^-1 (or 5 mg·mL). Interestingly,… More >

GIUSEPPINA AMODIO¹, VALENTINA PAGLIARA¹, PAOLO REMONDELLI^1,*, ORNELLA MOLTEDO²

BIOCELL, Vol.46, No.5, pp. 1131-1137, 2022, DOI:10.32604/biocell.2022.018638

Abstract The endoplasmic reticulum (ER) is the site of entry of all proteins that function in the secretory pathway including the extracellular environment. Because it controls the folding of newly synthesized secretory proteins, the ER is indispensable for the maintenance of proteostasis in the secretory pathway. Within the ER and, in part, in post-ER compartments, the quality control of protein folding is under the regulation of the unfolded protein response (UPR) pathways. The UPR strategy is to enhance protein folding, increase the ER degradation pathway of misfolded proteins, and allow the exit from the ER of only correctly folded proteins. The… More >

Longsheng Hu, Xinru He, Pujie Wei, Bing Sun, Yongjun Fei, Die Hu^*

Phyton-International Journal of Experimental Botany, Vol.90, No.6, pp. 1661-1672, 2021, DOI:10.32604/phyton.2021.015871

Abstract Seedlings of Taxus chinensis var. mairei were used as experimental materials to study the adaptation of Piriformospora indica to this plant under water stress. The materials were divided into two groups, namely, with or without inoculation with P. indica. Each group was subjected to four different levels of water stress. Vitality and physiological and biochemical indexes of the roots of T. chinensis var. mairei were regularly measured. Under water stress, T. chinensis var. mairei had significantly decreased root vitality; root vitality was higher in inoculated roots than in uninoculated roots. Under intense water stress, the inoculated roots had a higher… More >

Shahida Akter, Israt Jahan, Md. Amir Hossain^*, Mohammad Anwar Hossain^*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 949-970, 2021, DOI:10.32604/phyton.2021.014411

Abstract Drought susceptibility and low genetic variability are the major constraints of lentil (Lens culinaris Medik.) production worldwide. Development of an efficient pre-field drought phenotyping technique and identification of diversified drought tolerant lentil genotype(s) are therefore vital and necessary. Two separate experiments were conducted using thirty diverse lentil genotypes to isolate drought tolerant genotype(s) as well as to assess their diversity. In both of the experiments, significant (p ≤ 0.01) variation in genotype (G), treatment (T) and G X T was observed for most of the studied traits. In experiment I, genotypes were examined for drought tolerance at the seedlings stage… More >

Zahoor Ahmad^1,2,*, Ejaz Ahmad Waraich³, Muhammad Zia ur Rehman⁴, Muhammad Ashar Ayub⁴, Muhammad Usman⁴, Hesham Alharby⁵, Atif Bamagoos⁵, Celaleddin Barutçular¹, Muhammad Ali Raza⁶, Fatih Çiğ⁷, Ayman El Sabagh^7,8,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 503-514, 2021, DOI:10.32604/phyton.2021.013588

Abstract Water is essential for the growth period of crops; however, water unavailability badly affects the growth and physiological attributes of crops, which considerably reduced the yield and yield components in crops. Therefore, a pot experiment was conducted to investigate the effect of foliar phosphorus (P) on morphological, gas exchange, biochemical traits, and phosphorus use efficiency (PUE) of maize (Zea mays L.) hybrids grown under normal as well as water deficit situations at the Department of Agronomy, University of Agriculture Faisalabad, Pakistan in 2014. Two different treatments (control and P @ 8 kg ha⁻¹ ) and four hybrids (Hycorn, 31P41, 65625,… More >