DONGHUA LIU1*, WUSHENG JIANG², QINGMIN MENG¹, JIN ZOU¹, JIEGANG GU¹, MUAI ZENG¹

BIOCELL, Vol.33, No.1, pp. 25-32, 2009, DOI:10.32604/biocell.2009.33.025

Abstract Different copper concentrations, as well as different exposure times, were applied to investigate both cytogenetical and ultrastructural alterations in garlic (Allium sativum L.) meristem cells. Results showed that the mitotic index decreased progressively when either copper concentration or exposure time increased. C-mitosis, anaphase bridges, chromosome stickiness and broken nuclei were observed in the copper treated root tip cells. Some particulates containing the argyrophilic NOR-associated proteins were distributed in the nucleus of the root-tip cells and the amount of this particulate material progressively increased with increasing exposure time. Finally, the nucleolar material was extruded from the nucleus into the cytoplasm. Also,… More >

IIZUKA YOKO¹, SAMIN HONG², CHAN YUN KIM², SEUNG KAB KIM², GONG JE SEONG²

BIOCELL, Vol.32, No.3, pp. 245-250, 2008, DOI:10.32604/biocell.2008.32.245

Abstract Agmatine, 2-(4-aminobutyl)guanidine, has been reported to have neuroprotective effects against various neuronal damages. In this study it was investigated whether agmatine pretreatment rescues the retinal ganglion cells from oxidative injury in vitro. After differentiation of transformed rat retinal ganglion cells (RGC-5 cell line) with staurosporine, agmatine (0.0 to 100.0 μM) pretreatment was performed for 2 hours. Subsequently, they were exposed to hydrogen peroxide (0.0 to 2.5 mM) as an oxidative stress. Cell viability was monitored for up to 48 hours with the lactate dehydrogenase (LDH) assay and apoptosis was examined by the terminal deoxynucleotide transferase-mediated terminal uridine deoxynucleotidyl transferase nick… More >

SAMIN HONG, KYOUNGSOO PARK, CHAN YUN KIM, GONG JE SEONG

BIOCELL, Vol.32, No.2, pp. 201-205, 2008, DOI:10.32604/biocell.2008.32.201

Abstract The effect of hypoxia on the release of tumor necrosis factor-α (TNF-α) in transformed rat retinal ganglion cells (RGCs) and the effect of agmatine on the hypoxia-induced production of TNF-α in RGCs were evaluated. RGCs were cultured under hypoxic conditions with 5% oxygen, with or without 100 μM agmatine. The expression levels of TNF-α and its receptor-1 (TNF-R1) were investigated by Western blot analysis. After 6 hours of hypoxia, we noted an increase in TNF-α production in RGCs. Agmatine significantly reduced TNF-α level after 12 hours of hypoxic treatment. The expression of TNF-R1 was not affected by the hypoxia or… More >

FRANCISCO CAPANI¹, EZEQUIEL SARACENO¹, VALERIA ROMINA BOTI¹, LAURA AON-BERTOLINO¹, JUAN CARLOS FERNÁNDEZ¹, FERNANDO GATO¹, MARIA SOL KRAUSE², LISANDRO GIRALDEZ³, MARK H. ELLISMAN⁴, HÉCTOR COIRINI^1,2

BIOCELL, Vol.32, No.1, pp. 1-8, 2008, DOI:10.32604/biocell.2008.32.001

Abstract Cellular and subcellular organization and distribution of actin filaments have been studied with various techniques. The use of fluorescence photo-oxidation combined with phalloidin conjugates with eosin has allowed the examination of the precise cellular and subcellular location of F-actin. Correlative fluorescence light microscopy and transmission electron microscopy studies of F-actin distribution are facilitated with this method for morphological and physiological studies. Because phalloidin-eosin is smaller than other markers, this method allows the analysis of the three-dimensional location of F-actin with high-resolution light microscopy, three-d serial sections reconstructions, and electron tomography. The combination of selective staining and three-dimensional reconstructions provide a… More >

H.R. CONTRERAS¹, V. PAREDES¹, B. URQUIETA², L. DEL VALLE⁴, E. BUSTOS-OBREGÓN³

BIOCELL, Vol.30, No.3, pp. 423-429, 2006, DOI:10.32604/biocell.2006.30.423

Abstract Parathion® is an organophosphorate pesticide amply used in agriculture. Many alterations induced by organophosphorate pesticides have been described, such as: cytogenetic alterations in germinal cells, oligozoospermia and teratozoospermia in the mouse. The effect of Parathion®, both pure (PP) and commercial (PC), on mouse interstitial cell testosterone production was evaluated in vivo and in vitro. Male mice were intraperitoneally injected with a single dose of 1/3 LD50 of Parathion®, both PP and PC. The animals were sacrificed at 1, 8 and 40 days post injection to evaluate the impact of disrupting testosterone production on spermatogonia, spermatocytes and elongated spermatids. The plasma… More >

CARLOS A. MARTÍN, JOSÉ MIGUEL SURUR, MARCELA N. GARCÍA, FÉLIX CORRONS, AMADO F. BADRÁN

BIOCELL, Vol.29, No.1, pp. 33-38, 2005, DOI:10.32604/biocell.2005.29.033

Abstract Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 ± 16.1 SE; AgNORs 77.3… More >

GRACIELA CRISTINA CALABRESE*, ROSA WAINSTOK**

BIOCELL, Vol.28, No.3, pp. 251-258, 2004, DOI:10.32604/biocell.2004.28.251

Abstract Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular basis of differentiation pathways of endothelial cells, that enables its morphological heterogeneity. The purpose of this study was to analyze the pattern of PECAM-1 expression, employing monoclonal antibody MEC 13.3, in cellular suspensions obtained from different mouse organs at pre and postnatal stages.
Fluorescence activated cell sorter analysis showed a different profile of the glycoprotein… More >

C. D. GAMARRA-LUQUES, I. A. VEGA, E. KOCH, A. CASTRO-VAZQUEZ

BIOCELL, Vol.28, No.2, pp. 155-164, 2004, DOI:10.32604/biocell.2004.28.155

Abstract A new species of cyclopoid copepod, Ozmana huarpium, is described as a symbiont to Pomacea canaliculata (Lamarck 1822) (Caenogastropoda, Ampullariidae). Rather large numbers (about one hundred copepods per snail) were found, although there was no evidence of harm to the host. To our knowledge, O. haemophila (symbiont to P. maculata), and the currently described species, O. huarpium, are the only copepod species ever recorded as endosymbionts to freshwater invertebrates. While O. haemophila is restricted to the haemocoel of its host, O. huarpium predominate in the penis sheath, the ctenidium and the mantle cavity, figuring in these pallial organs 63-65 %… More >

S.K. VILA*, H.Y. REY, L.A. MROGINSKI

BIOCELL, Vol.28, No.1, pp. 35-41, 2004, DOI:10.32604/biocell.2004.28.035

Abstract In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10 – 15 years old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) supplemented with 1mg.l^-1 benzylaminopurine (BAP) + 0.1mg.l^-1 kinetin (KIN) + 3 mg.l^-1 adenine sulphate (ADS).
The rachis of the leaflets of the completely expanded leaves was found to be the most responsive tissue, in most of the genotypes… More >

ALICIA KOHLI¹ , STELLA M. PEZZOTTO² , GRACIELA GARCIA³ , LEONOR C. POLETTO².

BIOCELL, Vol.35, No.2, pp. 51-58, 2011, DOI:10.32604/biocell.2011.35.051

Abstract The embryological, structural and functional unit of the dentine-pulp complex shares the odontoblast, located in the border of the dentine pulp, with basal nuclei and organelles. The odontoblast process emerges from its apical pole. It is formed by microtubules, microfilaments and vesicles covered by membranes penetrating the dentinal tubules, isolated from the inter-tubular matrix, along the extent of the dentine. The objective of this study was to evaluate the efficacy of three staining techniques: hematoxylin-eosin, periodic acid-Schiff and Schmorl, by staining the process, from beginning to end, and compare the results with the erosion technique. Thirty human teeth were employed… More >