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  • Open Access

    ARTICLE

    The uptake of cadmium by Allium cepa var. agrogarum L. and its effects on chromosome and nucleolar behavior in root tip cells

    Wang QL, DH Liu, JY Yue

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 155-161, 2016, DOI:10.32604/phyton.2016.85.155

    Abstract Allium cepa var. agrogarum L. seedlings are sensitive to Cd stress. We used fluorescence imaging to indicate that Cd2+ was localized in cytoplasm in the epidermis of the basal parts of root and vascular tissues after Cd treatment. The nucleoli and the cell walls were the first storage sites of Cd2+. When Cd exposure was prolonged, severe irregularly-shaped nuclei were induced. We used silver nitrate staining to analyze the effects of different concentrations (1–300 μM) of cadmium chloride on chromosome, nucleolus and nucleolus organizer regions (NORs) in root tip cells. Cd2+ induced c-mitosis, chromosome bridges, chromosome stickiness and micronuclei. More… More >

  • Open Access

    ARTICLE

    Changes in soil organic carbon in the upper Heihe river basin, China

    Qin YY1,2, F Qi1, NM Holden3, JJ Cao4

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 149-154, 2016, DOI:10.32604/phyton.2016.85.149

    Abstract The Heihe River Basin is a globally significant carbon pool, but its soil organic carbon dynamics is poorly understood. Soil samples taken between 2500 m and 4100 m revealed that the majority (>75%) of soil organic carbon was from 0-40 cm. It showed a negative relationship with pH and soil bulk density, and a positive relationship with altitude and soil water content, respectively. From 2005 to 2011, soil carbon content in the upper catchment decreased from 93 g/kg to 53 g/kg. These results suggest that policies should aim to reduce carbon loss by transferring it from the top- to the… More >

  • Open Access

    ARTICLE

    Dynamic growth of squash (Cucurbita pepo L.) on a substrate based on vermicompost in the greenhouse

    Orozco V JA1, E Galindo P2, MA Segura C1, M Fortis H1, P Preciado R1, P Yescas C1, JA Montemayor T1

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 117-124, 2016, DOI:10.32604/phyton.2016.85.117

    Abstract The aim of this study was to establish the dynamics of growth and allocation of photosynthates in squash (Cucurbita pepo L.) cultivated in a semiarid region under greenhouse conditions and in an organic substrate. Squash seeds were sown in two substrates (one mineral and other organic). The first consisted of a mixture of sand and pumice on a volume basis, while the organic consisted of sand and pumice mixed with vermicompost, both in plastic pots. The substrates were for two treatments [Inorganic (IT) and Organic Treatment (OT)] with sixteen repetitions on a completely randomized design. After emergence, plants were fertilized… More >

  • Open Access

    ARTICLE

    Nitrogen deposition influences the response of Potentilla tanacetifolia to phosphorus addition

    Yang G1,2, Z Zhang1, G Zhang3, H Zhang2, X Han3, CA Busso4

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 100-107, 2016, DOI:10.32604/phyton.2016.85.100

    Abstract Phosphorus is an essential macronutrient for all living plants and plant production. Simultaneously, atmospheric nitrogen deposition also affects plant productivity at a global scale. However, few studies have investigated how plants respond to P addition while simultaneously considering N deposition. We investigated plant biomass, nutrient status and stoichiometric ratios on Potentilla tanacetifolia in response to P fertilization under contrasting N addition rates in a typical meadow steppe in Inner Mongolia, China. Aboveground biomass of P. tanacetifolia increased under increasing levels of P fertilization under conditions of N addition. However, there was no significant change in biomass when only phosphorus was… More >

  • Open Access

    ARTICLE

    In vitro plant regeneration via indirect organogenesis from different explants of Lathyrus sativus L. and Lathyrus cicera L.

    Li RS1, YJ Tao2, FJ Liu2, X Hu2, QL Xu2, KY Li2

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 87-93, 2016, DOI:10.32604/phyton.2016.85.087

    Abstract The grass pea (Lathyrus sativus L.) and flatpod peavine (Lathyrus cicera L.) are the most economically important and widely cultivated Lathyrus species. However, their utilization is limited due to the presence of their endogenous toxin β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP). Thus, a β-ODAP free variety should be developed through some plant breeding technique like either mutational breeding or genetic-manipulation. In this circumstance, the plant regeneration of Lathyrus species becomes a bottleneck. In the present study, an efficient system for in vitro regeneration of L. sativus with high β-ODAP levels, and L. cicera with low β-ODAP levels, was developed from different explants… More >

  • Open Access

    ARTICLE

    Phytochemical quality of tomato Saladette produced with organic substrates under greenhouse conditions

    González B ML1, M Fortis H1, P Preciado R1, MA Segura C1, E Salazar Sosa1, JL García H2, JR Esparza R3

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 71-78, 2016, DOI:10.32604/phyton.2016.85.071

    Abstract The aim of the current study was to evaluate yield and phytochemical quality of tomato fruits produced using organic substrates under greenhouse conditions. The applied treatments (organic substrates) were: T1 (80% River sand + 20% Vermicompost; T2 (90% River sand + 10% Compost); T3 Steiner Solution (80% River sand + 20% Perlite); T4 (80% River sand + 5% Soil + 15% Vermicompost); T5 (85% River sand + 15% Treated manure), and T6 (80% River sand + 5% Soil + 15% Treated manure). We used a totally randomized experimental design, with four replicate treatments. Evaluated variables were: yield, relative content of… More >

  • Open Access

    ARTICLE

    Quality and capsaicinoid concentration on genotypes of Serrano pepper (Capsicum annuum L.) produced under organic fertilization

    Valadez Sánchez YM1, E Olivares Sáenz1, RE Vázquez Alvarado1, JR Esparza-Rivera3, P Preciado-Rangel4, RD Valdez-Cepeda5, JL García-Hernandez2

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 21-26, 2016, DOI:10.32604/phyton.2016.85.021

    Abstract The aim of this study was to evaluate the effect of three organic fertilization sources on the agronomic and biochemical qualities of four genotypes of Serrano pepper. The experiment was performed at the field in Gómez Palacio, Durango (México). The capsaicinoids (CAP´s) are compounds responsible of the pungency of the peppers, and they are valuable for health and food and cosmetic food. The organic treatments applied were: solarized bovine manure (60 Mg/ha), vermicompost (10 Mg/ha) and poultry manure (5 Mg/ ha). A non-fertilized treatment was used as control. The evaluated variables were: length, diameter and individual weight on fruits, and… More >

  • Open Access

    ARTICLE

    Evaluation of a vermicompost and leachates on Solidago x hybrida and organic carbon mineralization under aerobic incubations

    Ramírez Gerardo MG1, MA Chávez-García2, J Mejía-Carranza2

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 397-406, 2015, DOI:10.32604/phyton.2015.84.397

    Abstract In the floriculture region of Tenancingo in the State of Mexico, the application of stabilized organic matter, such as vermicompost and leachates, contributes to improve the quality of the soil and plant nutrition. However, it is important to know the chemical composition of a vermicompost and the mineralization process. This is because the amount and speed of nutrient release which will be available to the crop will depend on that knowledge. The objective of the study was to evaluate the effect of the application of a vermicompost and leachates on various quantitative variables of Solidago x hybrid, and the mineralization… More >

  • Open Access

    ARTICLE

    Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant

    Guan Z-J1,3,4*, S-B Lu2*,Y-L Huo5, B Guo3, Z-P Guan6, Y-H Wei3

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 351-357, 2015, DOI:10.32604/phyton.2015.84.351

    Abstract Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until… More >

  • Open Access

    ARTICLE

    Effect of humic acids from Leonardite on the stability of soil aggregates and melon roots under greenhouse conditions

    Gutiérrez C JL1, G González C2, MA Segura C3, I Sánchez C2, JA Orozco V3, M Fortis H3

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 298-305, 2015, DOI:10.32604/phyton.2015.84.298

    Abstract Leonardite is an oxidized form of lignite carbon, which is obtained from fossilized organic materials. Such materials are used for the extraction of humic acids (HA). The result of the addition of HA of organic origin on soil structure is known; however, the effects of adding HA of Leonardite on soil structure have been scarcely investigated. The objectives of this research were (1) to determine the influence of humic acids derived from Leonardite in increasing the aggregate stability of an Aridisol under greenhouse conditions, and (2) evaluate the morphology of the root xylem during the phenological development of melon plants… More >

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