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  • Open Access

    ARTICLE

    Characterization and Expression of Ammonium Transporter in Peach (Prunus persica) and Regulation Analysis in Response to External Ammonium Supply

    Meiling Tang1,2,#, Yuhe Li1,3,#, Yahui Chen1,4, Lei Han1,3, Hongxia Zhang1,3, Zhizhong Song1,3,4,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.4, pp. 925-941, 2020, DOI:10.32604/phyton.2020.011184 - 09 November 2020

    Abstract As the preferred nitrogen (N) source, ammonium (NH4+ ) contributes to plant growth and development and fruit quality. In plants, NH4+ uptake is facilitated by a family of NH4+ transporters (AMT). However, the molecular mechanisms and functional characteristics of the AMT genes in peach have not been mentioned yet. In this present study, excess NH4+ stress severely hindered shoot growth and root elongation, accompanied with reduced mineral accumulation, decreased leaf chlorophyll concentration, and stunned photosynthetic performance. In addition, we identified 14 putative AMT genes in peach (PpeAMT). Expression analysis showed that PpeAMT genes were differently expressed in peach… More >

  • Open Access

    ARTICLE

    Utilization of Bayer Red Mud Derived from Bauxite for Belite-Ferroaluminate Cement Production

    Yanrong Zhao1,2,3,#, Ping Chen1,4,*,#, Shifeng Wang5, Yaxiong Ji3,4, Yuanhao Wang3,4,*, Bolin Wu1,2, Rongjin Liu1

    Journal of Renewable Materials, Vol.8, No.11, pp. 1531-1541, 2020, DOI:10.32604/jrm.2020.011462 - 28 September 2020

    Abstract Bayer red mud (BRM) is a kind of industrial solid waste characterized by huge volume and high alkalinity. Its disposal generates serious environmental pollution and occupies a large number of farmland. The utilization and recycling of BRM is currently a crucial issue and needs to be addressed as soon as possible. The chemical composition of BRM is similar to cement clinker. In this study, the feasibility of preparing Belite-ferroaluminate clinker (BFAC) with different BRM was explored. The physical properties, mechanics performance, radioactivity levels and trace harmful metals leaching were measured. XRD, BEI and EDS were… More >

  • Open Access

    ARTICLE

    Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid

    YUNZE XUAN1,#, BIN JIN1,#, SAYAN DEB DUTTA5,#, MENGMENG LIU2, ZAIXIAN SHEN1, XIWEN LIU3, YANG KANGJUAN4,*, LIM KI-TAEK5,*

    BIOCELL, Vol.44, No.3, pp. 389-400, 2020, DOI:10.32604/biocell.2020.09170 - 22 September 2020

    Abstract Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth, leading to the destruction of tooth-supporting tissues and loss of alveolar bone. Suberoylanilide hydroxamic acid (SAHA), a class of histone deacetylase (HDAC) inhibitor, has the potential to stimulate osteoblast differentiation by acetylating histone proteins, and thus suppressing the expression of adipogenic and chondrogenic genes. However, the effect of SAHA on the differentiation of human periodontal ligament stem cells (hPDLSCs) is yet to be elucidated. Herein, we investigated the effects of SAHA on in vitro More >

  • Open Access

    REVIEW

    Effect of structure and function of paraoxonase-1 (PON-1) on organophosphate pesticides metabolism

    HAZAEL RAMIRO CEJA-GÁLVEZ1, ERANDIS DHENI TORRES-SÁNCHEZ1, JUAN HERIBERTO TORRES-JASSO2, ARTURO VILLALOBOS ORNELAS1, JOEL SALAZAR-FLORES1,*

    BIOCELL, Vol.44, No.3, pp. 363-370, 2020, DOI:10.32604/biocell.2020.09147 - 22 September 2020

    Abstract Paraoxonase-1 (PON1) is an important enzyme in various pathologies such as pesticide poisoning, diabetes, atherosclerosis, neuronal disorders, and cancer, due to its multifunctional activity since it acts on different metabolites. However, one of its main functions is the hydrolysis of organophosphate (OP) compounds from pesticides that cause fatal poisoning at the level of the central nervous system (CNS). The objective of this review was to investigate whether the structure, genetics, and function of PON1 affect the metabolism of organophosphate pesticides or other abnormalities. Information was selected from articles in the database PubMed–NCBI (https://www.ncbi.nlm.nih.gov/ pubmed/) with More >

  • Open Access

    ARTICLE

    Age-related modifications of macrophages influenced by “inflammageing” in graft vs. host disease

    YAQUN HONG1,2, BO WAN3, XIAOFAN LI1,4,*

    BIOCELL, Vol.44, No.2, pp. 237-246, 2020, DOI:10.32604/biocell.2020.08887 - 27 May 2020

    Abstract Most studies focus on the adaptive immune cells in the GVHD pathogenesis, while little is known about innate immune cells in GVHD occurrence and development, especially macrophages. Meanwhile, a higher incidence of graft versus host disease (GVHD) is also found in the elderly patients. Though advances have been made in the modification of macrophages influenced by the inflamm-ageing, there is still no review on the role of macrophages in GVHD and the association between GVHD and the altered macrophages by inflamm-ageing. In this review, we focus on the potential age-related modifications of macrophage in GVHD, More >

  • Open Access

    ARTICLE

    Synergistic combination of colistin with imipenem, amikacine or ciprofloxacin against Acinetobacter baumannii and Pseudomonas aeruginosa carbapenem-resistant isolated in Annaba hospital Algeria

    SAIDA MELIANI1, SOUMAYA TOUMI1, HEYTHEM DJAHOUDI2, KHALED DEGHDEGH3, KAMEL AMOURA4, ABDELGHANI DJAHOUDI5,*

    BIOCELL, Vol.44, No.2, pp. 175-182, 2020, DOI:10.32604/biocell.2020.09097 - 27 May 2020

    Abstract Objective: The aim of this study is to detect in vitro the synergetic activity of colistin in combination with imipenem, amikacin or ciprofloxacin, at sub-inhibitory concentrations, against carbapenems-resistant (CR) Acinetobacter baumannii and Pseudomonas aeruginosa strains isolated from various wards in Annaba teaching hospital in eastern Algeria.
    Materials and Methods: The minimal inhibitory concentrations (MIC) were determined by broth macrodilution (BMD). Carbapenemase encoding genes were screened using polymerase chain reaction (PCR). The activity of colistin in combination with second antibiotic was evaluated by the Checkerboard Technique.
    Results: 39 CR P. aeruginosa and 21 CR A. baumanni strains where collected. The MIC values ranging… More >

  • Open Access

    ARTICLE

    Dephosphorylated mutations affect the protein-protein interactions of ERF in Populus simonii x P. nigra

    Yao SUN, Yao LI, Xin SUN, Qiong WU, Lei WANG*

    BIOCELL, Vol.44, No.1, pp. 117-126, 2020, DOI:10.32604/biocell.2020.08242 - 01 March 2020

    Abstract Phosphorylation is a common type of post-translational modification (PTM). It plays a vital role in many cellular processes. The reversible phosphorylation and dephosphorylation affect protein structures and proteinprotein interactions. Previously, we obtained five proteins that interact with ethylene-responsive factor (ERF) from the cDNA library of Populus simonii x Populus nigra. To further investigate the effect of dephosphorylation of PsnERF on its protein binding ability, we generated different phosphorylation states of PsnERF and demonstrated their protein binding capacity by the yeast two-hybrid assay (Y2H). The secondary structures and 3D structures of PsnERF, ERFm, TrunERF, and psnerf197/198/202a were predicted More >

  • Open Access

    ARTICLE

    Isolation of Thermally Stable Cellulose Nanocrystals from Spent Coffee Grounds via Phosphoric Acid Hydrolysis

    Brody A. Frost, E. Johan Foster*

    Journal of Renewable Materials, Vol.8, No.2, pp. 187-203, 2020, DOI:10.32604/jrm.2020.07940 - 01 February 2020

    Abstract As the world's population exponentially grows, so does the need for the production of food, with cereal production growing annually from an estimated 1.0 billion to 2.5 billion tons within the last few decades. This rapid growth in food production results in an ever increasing amount of agricultural wastes, of which already occupies nearly 50% of the total landfill area. For example, is the billions of dry tons of cellulose-containing spent coffee grounds disposed in landfills annually. This paper seeks to provide a method for isolating cellulose nanocrystals (CNCs) from spent coffee grounds, in order… More >

  • Open Access

    ARTICLE

    Extended hospital stay after radical cystectomy with enhanced recovery protocol

    Hatim Thaker, Saum Ghodoussipour, Mateen Saffarian, Akbar Ashrafi, Gus Miranda, Jie Cai, Anne K. Schuckman, Monish Aron, Mihir Desai, Inderbir S. Gill, Siamak Daneshmand, Hooman Djaladat

    Canadian Journal of Urology, Vol.26, No.1, pp. 9654-9659, 2019

    Abstract Introduction: To evaluate the reasons leading to an extended hospital stay (EHS) in patients undergoing radical cystectomy (RC) with postoperative enhanced recovery after surgery (ERAS) protocol.
    Materials and methods: A total of 509 patients underwent RC and urinary diversion with ERAS between May 2012 and March 2017. The protocol includes no bowel preparation, early feeding, predominantly non-narcotic pain control, and μ opioid antagonists. Non-consenting/lost to follow-up patients, and those with non-urothelial carcinoma were excluded. We defined EHS as ≥ 5 postoperative days and compared the cohort to those with a length of stay (LOS) of ≤ 4… More >

  • Open Access

    ARTICLE

    Effect of Synthesis Parameters on Phosphoric Acid Doped Polybenzimidazole Membranes: A Parametric Evaluation from Microstructural Level to Fuel Cell Performance

    ÇAĞLA GÜL GÜLDİKEN, LEVENT AKYALÇIN, HASAN FERDİ GERÇEL*

    Journal of Polymer Materials, Vol.36, No.4, pp. 337-349, 2019, DOI:10.32381/JPM.2019.36.04.4

    Abstract In the present study, polybenzimidazole (PBI) polymer which has a great importance in polymer electrolyte membrane (PEM) research was synthesized via the solution polycondensation method, under various synthesis conditions to put forward the effect of the synthesis parameters on the properties of the synthesized polymer and consequently to the quality of the prepared polymer electrolyte membrane. The synthesized polymers were characterized in terms of their chemical structure, thermal degradation behaviour, and molecular weight by Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance spectroscopy (1 H NMR), thermogravimetric analysis (TGA). The Mechanical properties, acid-doping level capacity, proton More >

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