Karen Hinsley, Margaret Evans-Langhorst, Courtney Porter, Stephanie Chandler, Christina VanderPluym, John Triedman, Vassilios J. Bezzerides

Congenital Heart Disease, Vol.13, No.2, pp. 222-225, 2018, DOI:10.1111/chd.12551

Abstract Objective: This quality improvement study was implemented to demonstrate consistent and reliable post procedure anticoagulation for patients undergoing left-sided ablations. We evaluated the safety and efficacy of anticoagulation practice during a transition from anticoagulation with overnight infusion of unfractionated heparin to a single subcutaneous injection of low molecular weight heparin.
Methods: Outcomes for patients who received unfractionated heparin from January 2014 to October 2014, were compared with outcomes of patients who received low molecular weight heparin from October 2014 to October 2015. Complications prepractice and postpractice change were documented and compared to establish confidence in the practice change and confirm… More >

Uri Pollak^1,2,3, David Mishaly^3,4, Gili Kenet^3,5, Amir Vardi^1,3

Congenital Heart Disease, Vol.13, No.1, pp. 16-25, 2018, DOI:10.1111/chd.12557

Abstract Heparin-induced thrombocytopenia (HIT) is a life-threatening complication of heparin therapy. The risk for HIT correlates with the cumulative dosage of heparin exposure. In Fontan patients, recurrent systemic anticoagulation, traditionally with heparin, is used to alleviate the thrombotic complications that may occur postoperatively when the venous pressure rises and the systemic venous flow into the pulmonary arteries becomes sluggish, putting them at increased risk. As a pressure gradient-dependent circulation, elevation in systemic venous pressure, most often by venous thrombosis, contributes to circuit failure. Therefore, when HIT complicates patients after the Fontan procedure, it is associated with a high thrombotic morbidity and… More >

M. I. ROSATTI, M. T. BECONI, M. CÓRDOBA

BIOCELL, Vol.28, No.3, pp. 311-316, 2004, DOI:10.32604/biocell.2004.28.311

Abstract Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acrosin at pH 8. Capacitation with heparin and quercitin failed to induce conversion of proacrosin to acrosin. An increase in acrosin activity produced by the presence of progesterone, in a dose-dependent manner, was related with the induction of true acrosome reaction. The total level of acrosin activity registered showed… More >

Qi Dang¹, Chun-Gong Li¹, Xin-Xin Jin¹, Ya-Jin Zhao¹, Xiang Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 146-146, 2019, DOI:10.32604/mcb.2019.07074

Abstract Endotoxin is a a very powerful and toxic inflammatory stimulator usually leading to the sepsis occurred. In order to remove endotoxin better through hemoperfusion, it is a pretty choice to increase the length of molecular linker on adsorbents. In this study, we chose the heparin as a molecular linker because of its being anticoagulant linear polysaccharide. Heparin as a linker was covalently immobilized on the chloromethylated polystyrene microspheres (Ps) and then connected with L-phenylalanine (Phe) forming the Ps-Hep-Phe structure to adsorbed endotoxin better. The property of microspheres was characterized by Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, zeta potential and… More >

Ying Mao¹, Chaojing Li¹, Peng Ge¹, Fujun Wang^1,*, Lu Wang^1,*

Molecular & Cellular Biomechanics, Vol.15, No.3, pp. 143-154, 2018, DOI: 10.3970/mcb.2018.03987

Abstract Heparin/ PEG-PCL core-shell microcarriers were fabricated in one-step via coaxial-electrospraying technology. Optimization of the coaxial-electrospraying processing is by controlling the PEG-PCL concentration, applied voltage, receiving distance, and feed rate. The influence of the electrospray parameters on microsphere morphology was studied by optical microscopy and scanning electron microscopy. The functional groups and components of the electrosprayed microspheres were characterized by Fourier transform infrared spectroscopy (FTIR). Transmission electron microscope (TEM) observation proved the core-shell structure of heparin-loaded PEG-PCL microspheres. Drug loading and releasing study demonstrated that PEG-PCL concentration could control the encapsulation efficiency and releasing activity of the heparin in the microspheres.… More >