Myoung Cheol Shin¹, Tae-Kyeong Lee², Dae Won Kim³, Joon Ha Park⁴, Moo-Ho Won¹, Choong-Hyun Lee^5,*, Ji Hyeon Ahn^6,*

BIOCELL, Vol.49, No.6, pp. 1071-1083, 2025, DOI:10.32604/biocell.2025.065506 - 24 June 2025

Abstract Background: Astrocyte endfeet (AEF) serves as a key element of the blood-brain barrier and is important for the survival and maintenance of neuronal function. However, the immunohistochemical and ultrastructural changes of AEF in the CA1 and CA3 areas of the hippocampus over time following cerebral ischemia-reperfusion (IR) injury have not been well elucidated. Objectives: We investigated chronological changes in AEF in the gerbil hippocampal CA1 area from 3 h to 10 days following transient forebrain ischemia (TFI), and examined their association with neuronal death and tissue repair following IR injury. Changes in the CA3 area… More >

Xiaojian Chen^1,2,#, Zhujiang Dai^1,#, Qiang Wang³, Wei Chen¹, Yun Liu^1,*, Zhongchuan Wang^1,*

BIOCELL, Vol.49, No.3, pp. 503-518, 2025, DOI:10.32604/biocell.2025.059286 - 31 March 2025

Abstract Background: Nudix Hydrolase 21 (NUDT21) is crucial for the regulation of alternative polyadenylation, with its reduced expression frequently resulting in a shortened mRNA 3^′ untranslated region (UTR), thereby enhancing the protein levels of downstream genes. Although NUDT21 is widely recognized for its tumor-suppressive function in various cancers, its involvement in colorectal cancer (CRC) remains poorly understood. Methods: The expression of NUDT21 in CRC and adjacent normal tissues was analyzed through qPCR, Western blot, and immunohistochemistry (IHC). Additionally, we investigated the correlation between NUDT21 expression and patient prognosis. With Cell Counting Kit-8 assay and Transwell assay, we… More >

KEWEI CHENG¹, LEI SHI¹, CAIWEN SHI¹, SHUANSHUAN XIE², CHANGHUI WANG^2,*

BIOCELL, Vol.48, No.8, pp. 1209-1221, 2024, DOI:10.32604/biocell.2024.049626 - 02 August 2024

Abstract Objective: To explore the role of polycystic kidney and hepatic disease 1-like 1 (PKHD1L1) in lung adenocarcinoma (LUAD). Methods: Bioinformatics tools were utilized to examine the clinical profile of PKHD1L1 and chromobox protein homolog 7 (CBX7) in LUAD. The Cell Counting Kit-8, colony formation, terminal deoxynucleotidyl transferase dUTP nick end labeling, Transwell, and wound-healing assays were carried out to assess the proliferative, apoptotic, invasive, and migrative capacities of the cells. Furthermore, the interrelation between PKHD1L1 and CBX7 was validated using a co-immunoprecipitation assay. A LUAD mice model was constructed by subcutaneous injection of A549 cells.… More >

FREDERICK H. SILVER^1,2,*, TANMAY DESHMUKH²

BIOCELL, Vol.48, No.4, pp. 525-540, 2024, DOI:10.32604/biocell.2024.047965 - 09 April 2024

Abstract All tissues in the body are subjected externally to gravity and internally by collagen fibril and cellular retractive forces that create stress and energy equilibrium required for homeostasis. Mechanotransduction involves mechanical work (force through a distance) and energy storage as kinetic and potential energy. This leads to changes in cell mitosis or apoptosis and the synthesis or loss of tissue components. It involves the application of energy directly to cells through integrin-mediated processes, cell-cell connections, stretching of the cell cytoplasm, and activation of the cell nucleus via yes-associated protein (YAP) and transcriptional coactivator with PDZ-motif… More >

Ain Raal^1,*, Linda Rusalepp², Tatiana Chiru³, Nicolae Ciobanu³, Kelly Talvistu¹, Michelle Shusta¹, Oleh Koshovyi^1,4, Tõnu Püssa²

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 2965-2979, 2023, DOI:10.32604/phyton.2023.042723 - 24 October 2023

Abstract The fruits of the Sea buckthorn (Hippophae rhamnoides L.) are a popular plant food and a valuable health product. Cultivating plants produces a lot of leaves with fine branches as an unnecessary residue, which needs valorization. The aim of the study was to estimate (by HPLC-MS/MS) the qualitative and quantitative content of polyphenolic compounds in different parts of H. rhamnoides (HR), and to determine the level of antioxidant activity of leaves and fruits (by DPPH, ABTS methods and ferrozine test). Up to 19 compounds were identified in different parts of HR. The fruits are rich in flavonoids, including… More >

Xinyi Xiao, Dongbo Pan^*, Jianjun Yuan

Intelligent Automation & Soft Computing, Vol.37, No.3, pp. 3179-3191, 2023, DOI:10.32604/iasc.2023.041208 - 11 September 2023

Abstract Neurological disorders like Alzheimer’s disease have a significant impact on the lives and health of the elderly as the aging population continues to grow. Doctors can achieve effective prevention and treatment of Alzheimer’s disease according to the morphological volume of hippocampus. General segmentation techniques frequently fail to produce satisfactory results due to hippocampus’s small size, complex structure, and fuzzy edges. We develop a new SC-Net model using complete brain MRI images to achieve high-precision segmentation of hippocampal structures. The proposed network improves the accuracy of hippocampal structural segmentation by retaining the original location information of More >

CHEN WANG, CHAOFAN HE, YUANYUAN GAO, KAIXIAN WANG, MENG LIANG^*

BIOCELL, Vol.47, No.1, pp. 155-164, 2023, DOI:10.32604/biocell.2023.024657 - 26 September 2022

Abstract Heat stress is an important influence on the male reproductive organs. Therefore, the effects of heat stress on genes or pathways related to the reproductive system of male mice were experimentally explored in this paper to further determine the effects of heat stimulation on mammals. Herein, models of heat-exposed mouse testicular tissue and heat-excited cells were successfully established. Many scorched vesicles were found after heat excitation of testis supporting cells, testicular mesenchymal (TM4) cells. Western blot, in situ terminal deoxynucleotide transferase dUTP Nick end labeling (TUNEL) and transmission electron microscopy showed that membrane rupture, mitochondrial damage More >

QING YIN¹, CAIHONG LIU¹, WENYING JIANG¹, HAIHUI GONG¹, CHUNYUN LI^1,2,3,*, ZUPING HE^1,2,3,*

BIOCELL, Vol.47, No.1, pp. 33-39, 2023, DOI:10.32604/biocell.2023.023567 - 26 September 2022

Abstract Yes-associated protein 1 (YAP1) is a downstream effector of the Hippo signaling pathway, and it is involved in tumorigenesis, tissue repair, growth, and development. In this review, the biological roles and the mechanisms of YAP1 in mediating stem cell fate decisions are discussed, including cell proliferation, differentiation, and apoptosis. In general, YAP1 promotes the proliferation and differentiation of stem cells, including embryonic stem cells and adult stem cells. It inhibits apoptosis by binding to the transcription factors, e.g., transcriptional enhanced associate domain (TEAD), Smad, runt-related transcription factor 1/2, p73, p63, and Erb84, to maintain tissue More >

Naigong Yu^*, Hejie Yu

CMES-Computer Modeling in Engineering & Sciences, Vol.131, No.2, pp. 1147-1169, 2022, DOI:10.32604/cmes.2022.019430 - 14 March 2022

Abstract The entorhinal-hippocampus structure in the mammalian brain is the core area for realizing spatial cognition. However, the visual perception and loop detection methods in the current biomimetic robot navigation model still rely on traditional visual SLAM schemes and lack the process of exploring and applying biological visual methods. Based on this, we propose a map construction method that mimics the entorhinal-hippocampal cognitive mechanism of the rat brain according to the response of entorhinal cortex neurons to eye saccades in recent related studies. That is, when mammals are free to watch the scene, the entorhinal cortex… More >

USAMA SHARIF AHMAD, KARTHIK SARAVANAN, HONG WAN^*

Oncology Research, Vol.29, No.6, pp. 377-391, 2021, DOI:10.32604/or.2022.026085 - 10 November 2022

Abstract The Yes-associated protein (YAP) is a downstream effector of the Hippo pathway and acts as a key transcription co-factor to regulate cell migration, proliferation, and survival. The Hippo pathway is evolutionarily conserved and controls tissue growth and organ size. Dysregulation and heterogeneity of this pathway are found in cancers, including oral squamous cell carcinoma (OSCC), leading to overexpression of YAP and its regulated proliferation machinery. The activity of YAP is associated with its nuclear expression and is negatively regulated by the Hippo kinase-mediated phosphorylation resulting in an induction of its cytoplasmic translocation. This review focuses More >