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  • Open Access

    ARTICLE

    Inhibition of Proliferation, Migration, and Invasion by Knockdown of Pyruvate Kinase-M2 (PKM2) in Ovarian Cancer SKOV3 and OVCAR3 Cells

    Yi Miao1, Meng Lu1, Qin Yan, Shuangdi Li, Youji Feng

    Oncology Research, Vol.24, No.6, pp. 463-475, 2016, DOI:10.3727/096504016X14685034103671

    Abstract Pyruvate kinase (PK) is a key enzyme in the process of glycolysis, catalyzing phosphoenolpyruvate (PEP) into pyruvate. Currently, PK isozyme type M2 (PKM2), one subtype of PK, has been proposed as a new tumor marker with high expression in various tumor tissues. Here we aimed to explore the effects of siRNAPKM2 on ovarian carcinoma (OC) cell lines SKOV3 and OVCAR3, in which PKM2 was notably expressed. PKM2 gene interference lentivirus vectors were built by miRNA transfection assay. siRNA-PKM2-transfected SKOV3 and OVCAR3 cells were evaluated for cell proliferation, cell cycle distribution, cell apoptosis, cell migration, and More >

  • Open Access

    ARTICLE

    Knockdown of Long Noncoding RNA uc.338 by siRNA Inhibits Cellular Migration and Invasion in Human Lung Cancer Cells

    Xuexin Gao*, Xuezhen Gao, Chao Li*, Yukun Zhang*, Lei Gao

    Oncology Research, Vol.24, No.5, pp. 337-343, 2016, DOI:10.3727/096504016X14666990347671

    Abstract Lung cancer remains a critical health concern worldwide. Long noncoding RNAs with ultraconserved elements have recently been implicated in human tumorigenesis. The present study investigated the role of ultraconserved element 338 (uc.338) in the regulation of cell proliferation and metastasis in human lung cancer. Our data showed that the expression of uc.338 in lung cancer was remarkably increased in vivo and in vitro. Depletion of uc.338 with specific siRNA interference retarded the cell proliferative rate in lung cancer cell lines NCI-H929 and H1688. Furthermore, knockdown of uc.338 caused cell cycle arrest in the G0/G1 phase in More >

  • Open Access

    ARTICLE

    Knockdown of Long Noncoding RNA PCAT6 Inhibits Proliferation and Invasion in Lung Cancer Cells

    Li Wan1, Lin Zhang1, Kai Fan, Zai-Xing Cheng, Quan-Chao Sun, Jian-Jun Wang

    Oncology Research, Vol.24, No.3, pp. 161-170, 2016, DOI:10.3727/096504016X14618564639178

    Abstract As a newly identified oncogenic long noncoding RNA (lncRNA), prostate cancer-associated transcript 6 (PCAT6) promoted cellular proliferation and colony formation of prostate cancer. However, the biological function of PCAT6 in lung cancer is still largely unknown. In this study, we found that PCAT6 is significantly increased in cancer tissues compared to normal tissues and positively correlates with metastasis of lung cancer in patients. We then examined PCAT6 expression in lung cancer cell lines and identified that PCAT6 expression was significantly elevated in lung cancer cells compared to normal human bronchial epithelial (NHBE) cells, especially in… More >

  • Open Access

    ARTICLE

    LINC00052 Promotes Gastric Cancer Cell Proliferation and Metastasis via Activating the Wnt/β-Catenin Signaling Pathway

    Yuqiang Shan1, Rongchao Ying1, Zhong Jia, Wencheng Kong, Yi Wu, Sixin Zheng, Huicheng Jin

    Oncology Research, Vol.25, No.9, pp. 1589-1599, 2017, DOI:10.3727/096504017X14897896412027

    Abstract Gastric cancer (GC) is one of the most common malignant tumors of the digestive system. The etiology of GC is complex, and much more attention should be paid to genetic factors. In this study, we explored the role and function of LINC00052 in GC. We applied qRT-PCR and Northern blot to detect the expression of LINC00052 and found it was highly expressed during GC. We also investigated the effects of LINC00052 on tumor prognosis and progression and found that LINC00052 indicated poor prognosis and tumor progression. By performing MTT, colony formation, and Transwell assays, we… More >

  • Open Access

    ARTICLE

    UCA1 Regulates the Growth and Metastasis of Pancreatic Cancer by Sponging miR-135a

    Xiaobo Zhang*, Feng Gao*, Lei Zhou*, Huaitao Wang*, Gang Shi, Xiaodong Tan*

    Oncology Research, Vol.25, No.9, pp. 1529-1541, 2017, DOI:10.3727/096504017X14888987683152

    Abstract Pancreatic cancer (PC) is a devastating malignant disease with a poor prognosis. This study aimed to investigate the role of urothelial carcinoma associated 1 (UCA1) in the progression of PC. Our results revealed that long noncoding RNA (lncRNA) UCA1 was overexpressed in PC tissues compared with adjacent histologically normal tissues. A downregulated level of UCA1 was also detected in five human PC cell lines (SW1990, BxPC-3, MiaPaCa-2, PANC-1, and CAPAN-1) compared with normal pancreatic duct epithelial HPDE cells. The proliferation of PC cells was inhibited after UCA1 was suppressed by a lentiviral vector. The cell… More >

  • Open Access

    ARTICLE

    FOXO1–MALAT1–miR-26a-5p Feedback Loop Mediates Proliferation and Migration in Osteosarcoma Cells

    Juntao Wang, Guodong Sun

    Oncology Research, Vol.25, No.9, pp. 1517-1527, 2017, DOI:10.3727/096504017X14859934460780

    Abstract miR-26a has been found to be downregulated in osteosarcoma (OS) when compared with normal control tissues and has been shown to suppress the malignant behaviors of OS cells. The underlying mechanism, nevertheless, remains unknown. In our study, the long noncoding RNA MALAT1, confirmed to be significantly upregulated in OS, is first shown to be capable of promoting proliferation and migration by directly suppressing miR-26a-5p in OS cells. In addition, we have identified forkhead box O1 (FOXO1) as a transcriptional factor of MALAT1 that can negatively regulate MALAT1. We have shown that MALAT1 promoted growth and More >

  • Open Access

    ARTICLE

    MicroRNA-133b Inhibits Proliferation, Cellular Migration, and Invasion via Targeting LASP1 in Hepatocarcinoma Cells

    Hui Li*, Zhigang Xiang*, Yan Liu*, Bin Xu*, Jianzhou Tang

    Oncology Research, Vol.25, No.8, pp. 1269-1282, 2017, DOI:10.3727/096504017X14850151453092

    Abstract MicroRNAs (miRs), a class of small noncoding RNAs, are key gene regulators through inducing translational repression or degradation of their target genes. However, the regulatory mechanism of miR-133b underlying hepatocellular carcinoma (HCC) growth and metastasis remains largely unclear. Here we found that miR-133b was significantly downregulated in HCC tissues and cell lines. Moreover, low miR-133b levels were significantly associated with the malignant progression of HCC. LASP1, upregulated in HCC tissues and cell lines, was then identified as a novel target of miR-133b in HCC HepG2 and Hep3B cells. Moreover, the increased expression of LASP1 was… More >

  • Open Access

    ARTICLE

    Upregulation of CD147 Promotes Metastasis of Cholangiocarcinoma by Modulating the Epithelial-to-Mesenchymal Transitional Process

    Paweena Dana*†‡, Ryusho Kariya, KulthidaVaeteewoottacharn*†, Kanlayanee Sawanyawisuth*†, Wunchana Seubwai†§, Kouki Matsuda, Seiji Okada, Sopit Wongkham*†

    Oncology Research, Vol.25, No.7, pp. 1047-1059, 2017, DOI:10.3727/096504016X14813899000565

    Abstract CD147 is a transmembrane protein that can induce the expression and activity of matrix metalloproteinases (MMPs). Expression of CD147 has been shown to potentiate cell migration, invasion, and metastasis of cancer. In this study, the critical role of CD147 in metastasis was elucidated using CD147-overexpressing cholangiocarcinoma (CCA) cells in vitro and in vivo. The molecular mechanism, demonstrated herein, supported the hypothesis that metastasis increased in CD147-overexpressing cells. Five CD147-overexpressing clones (Ex-CD147) were established from a low CD147-expressing CCA cell line, KKU-055, using lentivirus containing pReceiver-Lenti-CD147. The metastatic capability was determined using the tail vein injection… More >

  • Open Access

    ARTICLE

    MicroRNA-373 Promotes Growth and Cellular Invasion in Osteosarcoma Cells by Activation of the PI3K/AKT–Rac1–JNK Pathway: The Potential Role in Spinal Osteosarcoma

    Yufeng Liu*, Zhengliang Cheng, Feng Pan, Weigang Yan§

    Oncology Research, Vol.25, No.6, pp. 989-999, 2017, DOI:10.3727/096504016X14813867762123

    Abstract Spinal osteosarcoma (OS) has been proven to be more difficult to treat owing to potently malignant metastasis. The present study aimed to explore the functional role of microRNA (miR)-373 in cell growth and invasion of OS cells, as well as its underlying mechanism. The expression of miR-373 was analyzed in spinal OS tissues and cell lines. MG-63 cells were transfected with the miR-373 mimic or inhibitor and/or treated with the phosphoinositide 3-kinase (PI3K) (LY294002) inhibitor or Ras-related C3 botulinum toxin substrate 1 (Rac) guanosine triphosphate (GTPase) (NSC23766) inhibitor, and then the impact of miR-373 aberrant… More >

  • Open Access

    ARTICLE

    Protease Serine S1 Family Member 8 (PRSS8) Inhibits Tumor Growth In Vitro and In Vivo in Human Non-Small Cell Lung Cancer

    Chaonan Ma*1, Wei Ma*1, Nannan Zhou*, Na Chen*, Li An, Yijie Zhang*

    Oncology Research, Vol.25, No.5, pp. 781-787, 2017, DOI:10.3727/096504016X14772417575982

    Abstract Protease serine S1 family member 8 (PRSS8), a membrane-anchored serine protease, has been reported to be involved in the development of several human cancers. However, the role of PRSS8 in non-small cell lung cancer (NSCLC) pathogenesis remains unclear. The objective of this study was to investigate PRSS8 expression, biological function, and its related molecular mechanism in NSCLC. Our results showed that PRSS8 was expressed in a low amount in NSCLC cell lines. Ectopic expression of PRSS8 inhibited tumor growth in vitro and in vivo. Furthermore, ectopic expression of PRSS8 inhibited the migration and invasion of More >

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