Jiménez-Martínez JH¹, MG Gutiérrez-Martínez², O Franco-Mora¹, A González-Huerta², AT Gutiérrez-Ibáñez²

Phyton-International Journal of Experimental Botany, Vol.82, pp. 107-112, 2013, DOI:10.32604/phyton.2013.82.107

Abstract Two accessions of wild grapevines (Vitis spp.) native to Puebla State, Nealticán and Teziutlán, and other two native to Temascaltepec (1 and 2), Mexico State, were cultured in vitro using both Murashige-Skoog (MS) and woody plant medium (WPM) mediums. There were no differences between mediums for shoot generation. However, differences were shown among accessions for explant vigour, callus development, number of roots, leaves and nodes per explant. Rooting was evaluated only in MS medium by adding naphthalene acetic acid (NAA), indole butyric acid (IBA) or indole acetic acid (IAA) at 0.5 mg/L. With the exception of Temascaltepec 2, NAA generated… More >

Sadia^1,3 B, PC Josekutty², SD Potlakayala², P Patel², S Goldman³, SV Rudrabhatla²

Phyton-International Journal of Experimental Botany, Vol.79, pp. 177-181, 2010, DOI:10.32604/phyton.2010.79.177

Abstract A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded for the genotype… More >

Torroba MC, HA Paccapelo, L Aguilera, J Mazzola

Phyton-International Journal of Experimental Botany, Vol.77, pp. 93-102, 2008, DOI:10.32604/phyton.2008.77.093

Abstract An optimized methodology for improving regeneration of maize plants by direct organogenesis was evaluated. Our objective was to embarobtain genetically homogeneous plants through in vitro methods to regenerate clumps of multiple shoots from shoot tips at high frequency. Cultures were initiated from shoot tips of experimental lines of maize on a Murashige & Skoog (MS) medium supplemented with 2 mg/l benziladenine. Two experimental forage maize lines were used, in which two frequencies of subcultures were evaluated, namely line L. 850 (every 15 or 30 days) and line L.769 (every 30 days). It was observed that the highest rate of regeneration… More >

Giménez¹ C, E de García², O Haddad³

Phyton-International Journal of Experimental Botany, Vol.77, pp. 65-79, 2008, DOI:10.32604/phyton.2008.77.065

Abstract Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with… More >

Ojeda-Zacarías¹ Ma del Carmen, Hugo A Luna-Olvera², Lilia H Morales-Ramos², María J Verde-Star², Teresa E Torres-Cepeda², Benito Pereyra-Alférez², Leobardo Iracheta-Donjuan³, Emilio Olivares-Sáenz⁴, Raúl Salazar-Sáenz⁴, Elizabeth Cárdenas-Cerda⁴

Phyton-International Journal of Experimental Botany, Vol.75, pp. 109-113, 2006, DOI:10.32604/phyton.2006.75.109

Abstract Pinus maximartinezii (Rzedowski) is an endemic endangered species, confined to a single population of approximately 2000 to 2500 mature trees. It covers about 400 ha in southern Zacatecas, Mexico. The success of tissue culture techniques for germplasm preservation depends on regeneration of cultures. The objective of this study was to achieve an in vitro proliferation protocol using organogenesis technique for Pinus maximartinezii. Mature seeds were surface sterilized in 6% H2O2 v/v. Isolated cotyledons and zygotic embryos were cultured on shoot induction media. DCR and GD media were supplemented with 0.3 and 0.5 mgl-1 BAP; 0.01 mgl-1 ANA and vitamin solution.… More >

C. Luna, P. Sansberro*, L. Mroginski, J. Tarragó

BIOCELL, Vol.27, No.2, pp. 205-212, 2003, DOI:10.32604/biocell.2003.27.205

Abstract Micropropagation of Ilex dumosa var. dumosa R. (“yerba señorita”) from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in ¹/₄ strength Murashige and Skoog medium (¹/₄ MS) plus 30 gr·L^-1 sucrose and supplemented with 4.4 µM BA. Further multiplication and elongation of the regenerated shoots were obtained by subculture in a fresh medium of similar composition with 1.5 gr·L^-1 sucrose. Rooting induction from shoots were achieved in two steps: 1) 7 days in ¹/₄ MS (30 gr·L^-1 sucrose, 0.25 % Phytagel^®) with 7.3 µM IBA and 2) 21 days in… More >

Concepción Marino¹, María T. Ponce^1,*, María E. Videla², Sonia Fioretti³, Miguel Cirrincione¹

BIOCELL, Vol.27, No.1, pp. 57-60, 2003, DOI:10.32604/biocell.2003.27.057

Abstract Glandularia perakii is a perennial species with beautiful violet flowers that grows in the stony soil of Mendocine pedemont. A plentiful and prolonged flowering confers it an important ornamental potential. In this paper, a method of propagation of G. perakii from nodal segments is reported. Proliferating microshoot cultures were obtained by placing nodal segment on Murashige and Skoog medium (MS) supplemented with 20 g.L^-1 of sucrose without growth regulators. In this medium multiplication rate after 20 days was 7.9. Rooted plants were acclimatized successfully . More >

MARÍA CECILIA JUÁREZ¹, CARLOS BERNARDO PASSERA^1,2

BIOCELL, Vol.26, No.3, pp. 319-324, 2002, DOI:10.32604/biocell.2002.26.319

Abstract The genus Opuntia is a valuable forage resource in arid and semiarid lands during periods of drought and shortage of herbaceous plants. However, absolute minimum temperatures in the plains of Mendoza represent a limiting factor to cultivate several species.
Opuntia ellisiana is a cold hardy species, so the goals of this study were to massively propagate it using in vitro culture techniques, and then to acclimatize plantlets obtained to field conditions.
Different sterilization protocols were tested. Areoles were isolated in laminar airflow cabinet, and cultured on Murashige-Skoog medium, supplemented with sucrose and different BAP and IBA combinations. Explants were… More >

L.A. CARO¹, P.A. POLCI¹ , L.I. LINDSTRÖM¹ , C.V. ECHENIQUE^1,2, L.F. HERNÁNDEZ^1,3

BIOCELL, Vol.26, No.1, pp. 25-33, 2002, DOI:10.32604/biocell.2002.26.025

Abstract Prosopis chilensis (Mol.) Stuntz (Algarrobo de Chile) is an important native tree species that can be grown in arid and semiarid regions for wood and forage production and environmental protection. Developing a simple and reliable in vitro protocol for cloning it would enable to improve it genetically. Explants of P.chilensis were taken from 4 months-old plants grown in the greenhouse or from adult trees grown in a natural environment. Nodal segments 1 – 2 cm long containing an axillary bud were selected from elongating shoots. These cuttings were aseptically cultured on two agar-solid basal media, MS or BTMm, and treated… More >

Jimarez-Montiel MJ¹, A Robledo-Paz¹, VM Ordaz-Chaparro², LI Trejo-Tellez², JC Molina-Moreno¹

Phyton-International Journal of Experimental Botany, Vol.87, pp. 94-104, 2018, DOI:10.32604/phyton.2018.87.094

Abstract An alternative method for improving the production of habanero chili is the tissue culture technique; however, the gelling agent, the high salt and sucrose concentrations used in the culture media raise production costs and limit the adaptation of the regenerated plants to greenhouse or field conditions. In this study, the effect of the substrates perlite-coconut fiber, coconut fiber-volcanic rock, vermiculite-perlite, and perlite-volcanic rock in conjunction with various culture media in in vitro plant regeneration from embryos was evaluated. The differentiation of adventitious shoots on substrates was scarcely observed or non-existent. Inducing the formation of shoots on agar and their development… More >