MARÍA LAURA FONTANA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY*

BIOCELL, Vol.33, No.3, pp. 179-186, 2009, DOI:10.32604/biocell.2009.33.179

Abstract With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of More >

MARIA TERESA MARTIN^2*, HILDA PEDRANZANI³, PATRICIA GARCÍA-MOLINERO², VALENTIN PANDO⁴, ROSARIO SIERRA-DE-GRADO¹

BIOCELL, Vol.33, No.3, pp. 141-148, 2009, DOI:10.32604/biocell.2009.33.141

Abstract Two independent parameters, epicotyl height (cm) and number of induced buds were studied on Pinus pinaster explants to analyse the effects of three phytohormones (6-benzylaminopurine, jasmonic acid, ethylene) which were combined or not in 11 different treatments. Epicotyle length diminished significantly in relation to the control medium (medium without exogen phytohormones) in presence of jasmonic acid, 6-benzylaminopurine or Ethephon (which is converted to ethylene in plants) in any of treatments. Concentrations of 100 μM of jasmonic acid and Ethephon had a greater inhibitory effect than the treatments with 10 μM. In addition to that, jasmonic acid… More >

Torroba MC, HA Paccapelo, L Aguilera, J Mazzola

Phyton-International Journal of Experimental Botany, Vol.77, pp. 93-102, 2008, DOI:10.32604/phyton.2008.77.093

Abstract An optimized methodology for improving regeneration of maize plants by direct organogenesis was evaluated. Our objective was to embarobtain genetically homogeneous plants through in vitro methods to regenerate clumps of multiple shoots from shoot tips at high frequency. Cultures were initiated from shoot tips of experimental lines of maize on a Murashige & Skoog (MS) medium supplemented with 2 mg/l benziladenine. Two experimental forage maize lines were used, in which two frequencies of subcultures were evaluated, namely line L. 850 (every 15 or 30 days) and line L.769 (every 30 days). It was observed that the More >

Giménez¹ C, E de García², O Haddad³

Phyton-International Journal of Experimental Botany, Vol.77, pp. 65-79, 2008, DOI:10.32604/phyton.2008.77.065

Abstract Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from More >

M.T. MARTIN^*, H.E. PEDRANZANI^**, R. SIERRA DE GRADO

BIOCELL, Vol.31, No.1, pp. 41-49, 2007, DOI:10.32604/biocell.2007.31.041

Abstract An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations.
Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven… More >

NÉSTOR CURVETTO1^1,2, PABLO MARINANGELI^1,2, GABRIELA MOCKEL²

BIOCELL, Vol.30, No.3, pp. 497-500, 2006, DOI:10.32604/biocell.2006.30.497

Abstract The micropropagation of Lilium longiflorum requires adequate equipment which may not be afforded by small laboratories or producers. In this work we compared traditional methodology with a protocol that included easily available elements to sterilize materials and culture media, together with addition of hydrogen peroxide (H₂O₂ ) into the nutrient media as chemical sterilizer. A series of H₂O₂ concentrations (0.005, 0.010, 0.015 and 0.020% p/v) was used to control contamination during in vitro establishment and subsequent cultivation; the explant organogenic response was also examined and compared to the traditional micropropagation technique. The level of culture contamination was within… More >

Ojeda-Zacarías¹ Ma del Carmen, Hugo A Luna-Olvera², Lilia H Morales-Ramos², María J Verde-Star², Teresa E Torres-Cepeda², Benito Pereyra-Alférez², Leobardo Iracheta-Donjuan³, Emilio Olivares-Sáenz⁴, Raúl Salazar-Sáenz⁴, Elizabeth Cárdenas-Cerda⁴

Phyton-International Journal of Experimental Botany, Vol.75, pp. 109-113, 2006, DOI:10.32604/phyton.2006.75.109

Abstract Pinus maximartinezii (Rzedowski) is an endemic endangered species, confined to a single population of approximately 2000 to 2500 mature trees. It covers about 400 ha in southern Zacatecas, Mexico. The success of tissue culture techniques for germplasm preservation depends on regeneration of cultures. The objective of this study was to achieve an in vitro proliferation protocol using organogenesis technique for Pinus maximartinezii. Mature seeds were surface sterilized in 6% H2O2 v/v. Isolated cotyledons and zygotic embryos were cultured on shoot induction media. DCR and GD media were supplemented with 0.3 and 0.5 mgl-1 BAP; 0.01 mgl-1 ANA and More >

R. MEDINA, M. FALOCI, M.A. MARASSI, AND L.A. MROGINSKI.

BIOCELL, Vol.28, No.1, pp. 13-20, 2004, DOI:10.32604/biocell.2004.28.013

Abstract An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins (BAP and KIN) and auxins (2,4-D and NAA). After four weeks of culture, multiple shoots were obtained. The best response was observed on MS supplemented with BAP 5 mg l^-1. Shoot clumps were multiplied in MS liquid medium containing More >

C. Luna, P. Sansberro*, L. Mroginski, J. Tarragó

BIOCELL, Vol.27, No.2, pp. 205-212, 2003, DOI:10.32604/biocell.2003.27.205

Abstract Micropropagation of Ilex dumosa var. dumosa R. (“yerba señorita”) from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in ¹/₄ strength Murashige and Skoog medium (¹/₄ MS) plus 30 gr·L^-1 sucrose and supplemented with 4.4 µM BA. Further multiplication and elongation of the regenerated shoots were obtained by subculture in a fresh medium of similar composition with 1.5 gr·L^-1 sucrose. Rooting induction from shoots were achieved in two steps: 1) 7 days in ¹/₄ MS (30 gr·L^-1 sucrose, 0.25 % Phytagel^®) with 7.3 µM IBA and 2) 21 days in More >

Concepción Marino¹, María T. Ponce^1,*, María E. Videla², Sonia Fioretti³, Miguel Cirrincione¹

BIOCELL, Vol.27, No.1, pp. 57-60, 2003, DOI:10.32604/biocell.2003.27.057

Abstract Glandularia perakii is a perennial species with beautiful violet flowers that grows in the stony soil of Mendocine pedemont. A plentiful and prolonged flowering confers it an important ornamental potential. In this paper, a method of propagation of G. perakii from nodal segments is reported. Proliferating microshoot cultures were obtained by placing nodal segment on Murashige and Skoog medium (MS) supplemented with 20 g.L^-1 of sucrose without growth regulators. In this medium multiplication rate after 20 days was 7.9. Rooted plants were acclimatized successfully . More >