Yuanyuan Li^1,#, Jinze Mu^2,#, Qingliang Li¹, Huabing Liu³, Xuefeng Yuan^2,*, Deya Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2079-2085, 2023, DOI:10.32604/phyton.2023.028237

Abstract Infection of plum bark necrosis stem pitting associated virus (PBNSPaV) has been reported in many Prunus species in several countries, causing significant economic losses. The very small proteins encoded by plant viruses are often overlooked due to their short sequences and uncertain significance. However, numerous studies have indicated that they might play important roles in the pathogenesis of virus infection. The role of small hydrophobic protein P6, encoded by the open reading frame 2 of PBNSPaV, has not been well explored. In this study, we amplified the P6 fragment from a PBNSPaV isolate by RT-PCR using specific primers and found… More >

YINGHUA LUO^1,#, YU ZHANG^2,#, TONG ZHANG^2,#, YANNAN LI², HUI XUE², JINGLONG CAO², WENSHUANG HOU², JIAN LIU², YUHE CUI², TING XU², CHENGHAO JIN^2,3,*

BIOCELL, Vol.47, No.5, pp. 965-975, 2023, DOI:10.32604/biocell.2023.027729

Abstract Marine collagen peptides (MCPs) are natural products prepared by hydrolyzing marine collagen protein through a variety of chemical methods or enzymes. MCPs have a range of structures and biological activities and are widely present in marine species. MCPs also have a small molecular weight, are easily modified, and absorbed by the body. These properties have attracted great interest from researchers studying antioxidant, anti-tumor, and anti-aging activities. MCPs of specific molecular weights have significant anti-tumor activity and no toxic side effects. Thus, MCPs have the potential use as anti-cancer adjuvant drugs. Free radicals produced by oxidation are closely related to human… More >

Kairui Liu^*, Xiaolin Wu^*, Xian Zang^†, Zejian Huang^*, Zeyu Lin^‡, Wenliang Tan^*, Xiang Wu^*, Wenrou Hu^*, Baoqi Li^*, Lei Zhang^*

Oncology Research, Vol.28, No.5, pp. 559-560, 2020, DOI:10.3727/096504020X16032056440102

Abstract Overexpression of the tumor necrosis factor receptor-associated factor 4 (TRAF4) has been detected in many cancer types and is considered to foster tumor progression. However, the role of TRAF4 in hepatocellular carcinoma (HCC) remains elusive. In this study, we found that TRAF4 was highly expressed in HCC cell lines and HCC tissues compared with normal liver cell lines and adjacent noncancerous tissues. TRAF4 overexpression in HCC tissues was correlated with tumor quantity and vascular invasion. In vitro studies showed that TRAF4 was associated with HCC cell migration and invasion. An in vivo study verified that TRAF4 overexpression facilitated metastasis in… More >

Adekunle Babajide Rowaiye^1,2, Moses Okonkwo Njoku³, Angus Nnamdi Oli², Sousan Akrami^4,5, Titilayo Asala¹, Ikemefuna Chijioke Uzochukwu⁶, Alex Akpa¹, Morteza Saki^4,5,*, Charles Okechukwu Esimone²

Oncologie, Vol.23, No.4, pp. 547-557, 2021, DOI:10.32604/oncologie.2021.019738

Abstract Nonsteroidal anti-inflammatory drugs have a number of side effects. However, some medicinal herbs have anti-inflammatory properties with few side effects. This study aimed to determine the effect of the aqueous extract of Gongronema latifolium (AEGL) leaves on the level of tumor necrosis factor-α (TNF-α), transforming growth factor-β (TGF-β), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) in rabbits. Enzyme-linked immunosorbent assay (ELISA) was performed to determine the effect of the AEGL on the activities of pro-inflammatory cytokine (TNFα), and regulatory cytokine (TGF-β) in rabbits. Also, a colorimetric method was performed to evaluate the levels of AST and ALT. Overall, the weight… More >

RAGHDA A. SOLIMAN¹, RANA A. YOUNESS^1,2,*, TAMER M. MANIE³, EMAD KHALLAF⁴, MOHAMED EL-SHAZLY¹, MONA ABDELMOHSEN⁵, HEBA HANDOUSSA¹, MOHAMED Z. GAD^6,*

BIOCELL, Vol.46, No.3, pp. 769-783, 2022, DOI:10.32604/biocell.2022.016636

Abstract Triple Negative Breast Cancer (TNBC) immunotherapy has recently shown promising approach. However, some TNBC patients presented with resistance. One of the reasons was attributed to the excessive release of cytokines at the tumor microenvironment (TME) such as Tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Fine regulation of these cytokines’ levels via non-coding RNAs (ncRNAs) might alleviate the immune quiescent nature of TME at TNBC tumors. However, the extrapolation of ncRNAs as therapeutic tools is highly challenging. Therefore, disentanglement the nature for the isolation of natural compounds that could modulate the ncRNAs and their respective targets is an applicable translational… More >

YIBIN RUAN¹, ZHONGMING XIE², QIONG LIU², LIXIAO ZHANG², XIKUI HAN², XIAOYAN LIAO², JIAN LIU^1,*, FENGGUANG GAO^2,*

BIOCELL, Vol.45, No.4, pp. 1059-1067, 2021, DOI:10.32604/biocell.2021.015261

Abstract Nicotine and menthol, agonists of nicotinic acetylcholine receptor (nAChR) and transient receptor potential melastatin type 8 (TRPM8), serve important roles in the prevention of cell death-involved neurodegenerative diseases. However, the potential synergistic effects of nicotine and menthol on anti-apoptotic ability are still uncertain. In the present study, the potential synergistic effects of nicotine and menthol on cisplatin or amyloid β_1-42 induced cell model of the neurodegenerative diseases were explored by assessing cell viability, TNF-α expression, caspase-3 activation, and the collapse of mitochondrial membrane potential in human SH-SY5Y neuroblastoma cells. Statistical significance was tested using Student’s t-test or one-way ANOVA with… More >

SAMIN HONG, KYOUNGSOO PARK, CHAN YUN KIM, GONG JE SEONG

BIOCELL, Vol.32, No.2, pp. 201-205, 2008, DOI:10.32604/biocell.2008.32.201

Abstract The effect of hypoxia on the release of tumor necrosis factor-α (TNF-α) in transformed rat retinal ganglion cells (RGCs) and the effect of agmatine on the hypoxia-induced production of TNF-α in RGCs were evaluated. RGCs were cultured under hypoxic conditions with 5% oxygen, with or without 100 μM agmatine. The expression levels of TNF-α and its receptor-1 (TNF-R1) were investigated by Western blot analysis. After 6 hours of hypoxia, we noted an increase in TNF-α production in RGCs. Agmatine significantly reduced TNF-α level after 12 hours of hypoxic treatment. The expression of TNF-R1 was not affected by the hypoxia or… More >

B.C. Heng*, K.J. Vinoth*, K. Lu*, X. Deng*, Z. Ge*, B.H. Bay**, T. Cao*

BIOCELL, Vol.31, No.3, pp. 405-410, 2007, DOI:10.32604/biocell.2007.31.405

Abstract We investigated the effects of prolonged heat shock treatment on human embryonic stem cell (hESC) viability. The hESC viability steadily declined with longer exposure to heat shock treatment (43ºC). After 4 h of exposure to heat shock at 43ºC, only 56.2 ± 1.5% of cells were viable. Viability subsequently declined to 37.0 ± 3.3% and 3.5 ± 0.7% after 8 h and 16 h, respectively of heat shock treatment at 43ºC. Transmission electron micrographs showed that the morphology of the dead/dying cells after heat shock treatment was characteristic of cellular necrosis with an uncondensed chromatin and a non-intact plasma membrane.… More >

G. Sciumè^∗,†, S.E. Shelton^‡, W.G. Gray^‡, C.T. Miller^‡, F. Hussain^§,¶, M. Ferrari^¶, P. Decuzzi^¶, B.A. Schrefler^∗,¶

Molecular & Cellular Biomechanics, Vol.9, No.3, pp. 193-212, 2012, DOI:10.3970/mcb.2012.009.193

Abstract Multiphase porous media mechanics is used for modeling tumor growth, using governing equations obtained via the Thermodynamically Constrained Averaging Theory (TCAT). This approach incorporates the interaction of more phases than legacy tumor growth models. The tumor is treated as a multiphase system composed of an extracellular matrix, tumor cells which may become necrotic depending on nutrient level and pressure, healthy cells and an interstitial fluid which transports nutrients. The governing equations are numerically solved within a Finite Element framework for predicting the growth rate of the tumor mass, and of its individual components, as a function of the initial tumor-to-healthy… More >