Qi Guo¹, Jianxiong Xu^2,*, Xuan Cai², Jiaojiao Xie¹, Siqi Wu¹

BIOCELL, Vol.49, No.5, pp. 907-924, 2025, DOI:10.32604/biocell.2025.063568 - 27 May 2025

Abstract Objectives: Weaning induces oxidative stress in pigs, increasing the risk of diarrhea and death. Intestinal damage is associated with obstructed intestinal cell cycles. To stop damage caused by reactive oxygen species (ROS), N-acetyl cysteine (NAC) has been widely employed. In this study, we examined changes in the intestinal cyclin of weaning piglets and assessed the impact of NAC on intestinal cell cycle arrest and intracellular signaling pathways. Methods: We conducted two animal experiments. In the first, we divided 12 litters of 120 newborn piglets into two groups: a control group and a weaning group. The… More >

Jung-Il Chae^1,#, Ji-Hye Seo^1,#, Goo Yoon², Young-Joo Jeon^3,*, Woo-Keun Kim^4,*

BIOCELL, Vol.49, No.4, pp. 647-663, 2025, DOI:10.32604/biocell.2025.062137 - 30 April 2025

Abstract Background: Picropodophllotoxin (PPT), a principal component of Podophyllum hexandrum root, demonstrates various beneficial biological activities in multiple cancer types, including antitumor and antiproliferative properties. Despite its known effects, the specific mechanisms by which PPT induces apoptosis in oral squamous cell carcinoma (OSCC) cells lack full clarification. Aims: This study aimed to evaluate the role of PPT in inducing apoptosis in OSCC cells by targeting signal transducer and activator of transcription 3 (STAT3) and to investigate the underlying molecular pathways. Methods: Human OSCC cell lines (HN22 and HSC4) were treated with PPT. Cell viability, colony formation, and apoptotic… More >

XIUWANG WEI¹, JIANBO LIANG¹, HUANWEN HUANG¹, DAMING YANG¹, XINXIN WANG¹, XIUJIA WANG¹, CHANGSHENG CHEN¹, KAIQIANG LI¹, TAISEN PANG¹, BIN HU¹, FENGNING WU^2,*

Oncology Research, Vol.33, No.1, pp. 161-169, 2025, DOI:10.32604/or.2024.048054 - 20 December 2024

Abstract Background: Transmembrane emp24 trafficking protein 3 (TMED3) is associated with the development of several tumors; however, whether TMED3 regulates the progression of prostate cancer remains unclear. Materials and Methods: Short hairpin RNA was performed to repress TMED3 in prostate cancer cells (DU145 cells) and in a prostate cancer mice model to determine its function in prostate cancer in vitro and in vivo. Results: In the present study, we found that TMED3 was highly expressed in prostate cancer cells. In vitro, shTMED3 treatment suppressed the proliferation, invasion, and migration and promoted the apoptosis of DU145 cells. Additionally, the Kyoto Encyclopedia… More >

XIAOQI WU^1,2,#, YECHUAN HE^1,2,#, YEQIN YUAN⁴, XIAN TAN^1,2, LIN ZHU^1,2, DANLING WANG^1,2,4, BINYUAN JIANG^3,4,*

BIOCELL, Vol.48, No.5, pp. 861-872, 2024, DOI:10.32604/biocell.2024.048758 - 06 May 2024

Abstract Background: Nasopharyngeal carcinoma (NPC) exhibits a significant prevalence in the southern regions of China, and paclitaxel (PTX) is frequently employed as a medication for managing advanced NPC. However, drug resistance is typically accompanied by a poor prognosis. Exploring the synergistic potential of combining multiple chemotherapeutic agents may represent a promising avenue for optimizing treatment efficacy. Methods: This study investigated whether 3-Methyladenine (3-MA) could potentiated the effect of PTX and its potential molecular mechanism. Samples were divided into the following categories: Negative control (NC) with the solvent dimethyl sulfoxide (DMSO, 0.5% v/v), PTX (400 nM), 3-MA… More >

WEN GE^1,2,#, YA LI^1,2,#, YUTING RUAN^1,2, NINGXIA WU^1,2, PEI MA^3,4, TONGPENG XU^3,4, YONGQIAN SHU^3,4, YINGWEI WANG^1,2, WEN QIU^1,2, CHENHUI ZHAO^3,4,*

Oncology Research, Vol.32, No.4, pp. 625-641, 2024, DOI:10.32604/or.2023.031053 - 20 March 2024

Abstract The cancer cell metastasis is a major death reason for patients with non-small cell lung cancer (NSCLC). Although researchers have disclosed that interleukin 17 (IL-17) can increase matrix metalloproteinases (MMPs) induction causing NSCLC cell metastasis, the underlying mechanism remains unclear. In the study, we found that IL-17 receptor A (IL-17RA), p300, p-STAT3, Ack-STAT3, and MMP19 were up-regulated both in NSCLC tissues and NSCLC cells stimulated with IL-17. p300, STAT3 and MMP19 overexpression or knockdown could raise or reduce IL-17-induced p-STAT3, Ack-STAT3 and MMP19 level as well as the cell migration and invasion. Mechanism investigation revealed… More > Graphic Abstract

MINJI PARK¹, CHULHWAN BANG², WON-SOO YUN³, YUN-MI JEONG^3,*

Oncology Research, Vol.32, No.2, pp. 273-282, 2024, DOI:10.32604/or.2023.044362 - 28 December 2023

Abstract Fucoidan, a sulfate polysaccharide obtained from brown seaweed, has various bioactive properties, including anti-inflammatory, anti-cancer, anti-viral, anti-oxidant, anti-coagulant, anti-thrombotic, anti-angiogenic, and anti-Helicobacter pylori properties. However, the effects of low-molecular-weight fucoidan (LMW-F) on melanoma cell lines and three dimensional (3D) cell culture models are not well understood. This study aimed to investigate the effects of LMW-F on A375 human melanoma cells and cryopreserved biospecimens derived from patients with advanced melanoma. Ultrasonic wave was used to fragment fucoidan derived from Fucus vesiculosus into smaller LMW-F. MTT and live/dead assays showed that LMW-F inhibited cell proliferation in both A375 cells More >

ZIYU LIU^1,2, AKIKO OKANO^3,4, EMIKO SANADA^1,3,4, YUSHI FUTAMURA^3,4, TOSHIHIKO NOGAWA^3,5, KOSUKE ISHIKAWA⁶, KENTARO SEMBA^7,8, JIANG LI⁹, XIAOMENG LI¹⁰, HIROYUKI OSADA^3,4,11,*, NOBUMOTO WATANABE^1,2,4,*

Oncology Research, Vol.31, No.5, pp. 655-666, 2023, DOI:10.32604/or.2023.030248 - 21 July 2023

Abstract

Myc belongs to a family of proto-oncogenes that encode transcription factors. The overexpression of c-Myc causes many types of cancers. Recently, we established a system for screening c-Myc inhibitors and identified antimycin A by screening the RIKEN NPDepo chemical library. The specific mechanism of promoting tumor cell metastasis by high c-Myc expression remains to be explained. In this study, we screened approximately 5,600 microbial extracts using this system and identified a broth prepared from Streptomyces sp. RK19-A0402 strongly inhibits c-Myc transcriptional activity. After purification of the hit broth, we identified compounds closely related to the aglycone

More > Graphic Abstract

SHU ZHAO^1,*, SHIXIN YE²

BIOCELL, Vol.47, No.6, pp. 1377-1388, 2023, DOI:10.32604/biocell.2023.023874 - 19 May 2023

Abstract Background: This paper aims to establish a light-controlled phosphorylation detection method at the Y785 site of tropomyosin receptor kinase A (TrkA) receptor in mammalian cells by using genetic code expansion technology and detecting the effects of optical activation of this site on the downstream MAPK/ERK pathway. The study is based on the current situation that the regulatory mechanism of TrkA phosphorylation has not been fully elucidated. Methods: Two photosensitive unnatural amino acids, p-azido-L-phenylalanine (AzF) and photo-caged tyrosine (ONB) were introduced into the TrkA-Y785 site by genetic code expansion technology and site-directed mutagenesis. Western blotting and laser More >

LUCÍA VELOZ^1,2, SANTIAGO A. BOSCH^1,3, GONZALO APARICIO^1,2,*, FLAVIO R. ZOLESSI^1,2,*

BIOCELL, Vol.46, No.3, pp. 639-644, 2022, DOI:10.32604/biocell.2022.018798 - 18 November 2021

Abstract Cell extrusion is an active mechanism to eliminate non-viable or supernumerary cells in healthy epithelia. It also plays a role in carcinogenesis, both in tumor growth (apical extrusion) and metastasis (basal extrusion). Embryonic tissues like the neuroepithelium, on the other hand, present rates of proliferation comparable to that of carcinomas, without the occurrence of cell extrusion. However, the downregulation or phosphorylation of actin-modulating proteins like MARCKS, causes extensive neuroepithelial apical cell extrusion. As changes in MARCKS proteins phosphorylation and expression have also been correlated to carcinogenesis, we propose here an integrated model for their functions More >

XINWEI WU¹, GUOYONG JIA^2,*, HONGNA YANG³, CONGCONG SUN², YING LIU², ZENGYAN DIAO²

BIOCELL, Vol.46, No.2, pp. 471-478, 2022, DOI:10.32604/biocell.2021.015701 - 20 October 2021

Abstract A progressive neurodegenerative disease, Alzheimer’s disease (AD). Studies suggest that highly expressed protein isoaspartate methyltransferase 1 (PCMT1) in brain tissue. In the current study, we explored the effects of neural stem cell-conditioned medium (NSC-CDM) on the PCMT1/MST1 pathway to alleviate Aβ_25-35-induced damage in SH-SY5Y cells. Our data suggested that Aβ25-35 markedly inhibited cell viability. NSC-CDM or Neural stem cell-complete medium (NSC-CPM) had a suppression effect on toxicity when treatment with Aβ_25-35, with a greater effect observed with NSC-CDM. Aβ_25-35 + NSC-CDM group exhibited an increase in PCMT1 expression. sh-PCMT1 markedly decreased cell proliferation and suppressed the protective More >