TAILIN WU^1,#, XIANG ZHOU^2,#, CANHUA YE¹, WENCAN LU¹, HAITAO LIN¹, YANZHE WEI¹, ZEKAI KE¹, ZHENGJI HUANG¹, JIANZHOU LUO¹, HUIREN TAO¹, CHUNGUANG DUAN^1,*

BIOCELL, Vol.46, No.2, pp. 495-503, 2022, DOI:10.32604/biocell.2022.015214

Abstract Differentiated macrophages have been proven to participate in the development of mesenchymal stem cells in different tissues. However, the regulatory processes remain obscure. Exosomes, which are key secretions of macrophages, have attracted increasing attention. Therefore, macrophage-derived exosomes may modulate the development of Bone marrow mesenchymal stem cells (BMMSCs). Different culture conditions were used to induce M1 polarization in THP1 cells. Subsequently, exosomes derived from unpolarized (M0) and polarized (M1) macrophages were isolated, BMMSCs were cultured with normal complete medium or inductive medium supplemented with M0 or M1 derived exosomes, and the osteogenic capacity of the BMMSCs was measured and analyzed.… More >

Maryam Jazayeri¹, Mohammad Ali Shokrgozar¹, Nooshin Haghighipour^1,2, Reza Mahdian³, Mehdi Farrokhi¹, Shahin Bonakdar¹, FereshtehMirahmadi¹, Tannaz Nourizadeh Abbariki

Molecular & Cellular Biomechanics, Vol.12, No.3, pp. 197-213, 2015, DOI:10.3970/mcb.2015.012.197

Abstract The osseous tissue repair and regeneration have great importance in orthopedic and maxillofacial surgery. Tissue engineering makes it possible to cure different tissue abnormalities using autologous grafts. It is now obvious that mechanical loading has essential role in directing cells to differentiation. In this study, the influence of cyclic uniaxial loading and its combination with chemical factors on expression of osteogenic markers was investigated. Rat bone marrow-derived stem cells were isolated and cultured. In one group cells were maintained in chemical induction medium. In another group cells were subjected to cyclic uniaxial strain with 3% amplitude and 0.3 Hz frequency… More >