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  • Open Access

    ARTICLE

    Effect of Cartilage Endplate on Cell Based Disc Regeneration: A Finite Element Analysis

    Yongren Wu, Sarah Cisewski, Barton L. Sachs, Hai Yao∗,†,‡

    Molecular & Cellular Biomechanics, Vol.10, No.2, pp. 159-182, 2013, DOI:10.3970/mcb.2013.010.159

    Abstract This study examines the effects of cartilage endplate (CEP) calcification and the injection of intervertebral disc (IVD) cells on the nutrition distributions inside the human IVD under physiological loading conditions using multiphasic finite element modeling. The human disc was modeled as an inhomogeneous mixture consisting of a charged elastic solid, water, ions (Na+ and Cl), and nutrient solute(oxygen,glucose and lactate) phases. The effect of the endplate calcification was simulated by a reduction of the tissue porosity (i.e., water volume faction) from 0.60 to 0.48. The effect of cell injection was simulated by increasing the cell density… More >

  • Open Access

    ARTICLE

    Bone Tissue Formation under Ideal Conditions in a Scaffold Generated by a Reaction-Diffusion System

    Marco A.Velasco, Diego A. Garzón-Alvarado

    Molecular & Cellular Biomechanics, Vol.10, No.2, pp. 137-157, 2013, DOI:10.3970/mcb.2013.010.137

    Abstract The design of porous scaffolds for tissue engineering requires methods to generate geometries in order to control the stiffness and the permeability of the implant among others characteristics. This article studied the potential of the reaction-diffusion systems to design porous scaffolds for bone regeneration. We simulate the degradation of the scaffold material and the formation of new bone tissue over canal-like, spherical and ellipsoid structures obtained by this approach. The simulations show that the degradation and growth rates are affected by the form of porous structures. The results have indicated that the proposed method has More >

  • Open Access

    ARTICLE

    Plant regeneration of Mullein Nightshade (Solanum donianum Walp.) from leaf explants

    O’Connor-Sánchez1 A, AV Domínguez-May1, MA Keb-Llanes1, YJ Peña-Ramírez2, VA Herrera-Valencia1

    Phyton-International Journal of Experimental Botany, Vol.79, pp. 25-29, 2010, DOI:10.32604/phyton.2010.79.025

    Abstract A reliable protocol for plant regeneration of Mullein Nightshade (Solanum donianum Walp.) was developed from in vitro leaf explants. They were cultured on Murashige and Skoog semisolid medium, supplemented with several combinations of zeatin riboside and 1-naphthaleneacetic acid for shoot regeneration. The formulation that originated most shoots per explant was 8.5 μM zeatin riboside in absence of 1-naphthalene acetic acid. Explants with shoots were transferred to Murashige and Skoog medium, with half the normal salt concentration and without plant growth regulators for elongation. Elongated shoots were individualized, and they rooted readily in half-strength Murashige and Skoog More >

  • Open Access

    ARTICLE

    Organogenesis and plant regeneration of Arachis villosa Benth. (Leguminosae) through leaf culture

    MARÍA LAURA FONTANA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY*

    BIOCELL, Vol.33, No.3, pp. 179-186, 2009, DOI:10.32604/biocell.2009.33.179

    Abstract With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of More >

  • Open Access

    ARTICLE

    Isolation of stem cells from adult rat kidneys

    YOUSOF GHEISARI1,2, MASOUD SOLEIMANI3*, SIROUS ZEINALI2, EHSAN AREFIAN1, AMIR ATASHI1,3, MAHIN NIKOUGOFTAR ZARIF4

    BIOCELL, Vol.33, No.1, pp. 33-38, 2009, DOI:10.32604/biocell.2009.33.033

    Abstract The kidney has an inherent ability for recovery and regeneration following acute damage. However, there has been much contention as to the source of regenerating renal cells. The aim of this study was to isolate and characterize these cells. Normal rat kidneys were minced and cells were isolated with collagenase I and were cultured in an expansion medium. Adherent cells were isolated and expanded for more than 120 days in vitro. These cells had the potential of trans-lineage differentiation into neural cells, adipocytes and osteocytes. These cells also expressed Nucleostemin, Cyclin D1, Notch1 and Survivin which More >

  • Open Access

    ARTICLE

    Plant micropropagation in experimental lines of forage maize coming from crossing Zea mays L. and Zea diploperennis Iltis, Doebley and Guzmán

    Torroba MC, HA Paccapelo, L Aguilera, J Mazzola

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 93-102, 2008, DOI:10.32604/phyton.2008.77.093

    Abstract An optimized methodology for improving regeneration of maize plants by direct organogenesis was evaluated. Our objective was to embarobtain genetically homogeneous plants through in vitro methods to regenerate clumps of multiple shoots from shoot tips at high frequency. Cultures were initiated from shoot tips of experimental lines of maize on a Murashige & Skoog (MS) medium supplemented with 2 mg/l benziladenine. Two experimental forage maize lines were used, in which two frequencies of subcultures were evaluated, namely line L. 850 (every 15 or 30 days) and line L.769 (every 30 days). It was observed that the More >

  • Open Access

    ARTICLE

    Wnt Signaling is Essential for Bone Regeneration

    J. B. Kim1, P. Leucht1,2, K. Lam1, C. A. Luppen1, D. ten Berg3, R. Nusse3, J. A. Helms1

    Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 201-201, 2006, DOI:10.32604/mcb.2006.003.201

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    The Effect of Processing Temperature on Mechanical Properties of Novel Silk Fibroin and Chitosan Blend Scaffolds for Musculoskeletal Regeneration

    C. N. Rios1, J. N. Augustine1, A. B. Mathur1

    Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 173-174, 2006, DOI:10.32604/mcb.2006.003.173

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    Adult Stem Cells and Skeletal Repair and Regeneration

    C. Niyibizi1, F. Li1, X. Wang1, X. Liao1

    Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 149-149, 2006, DOI:10.32604/mcb.2006.003.149

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    ACL Healing and Regeneration

    L. Yang1, K. L. Paul Sung1

    Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 147-147, 2006, DOI:10.32604/mcb.2006.003.147

    Abstract This article has no abstract. More >

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