MARÍA LAURA FONTANA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY*

BIOCELL, Vol.33, No.3, pp. 179-186, 2009, DOI:10.32604/biocell.2009.33.179

Abstract With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of More >

YOUSOF GHEISARI^1,2, MASOUD SOLEIMANI^3*, SIROUS ZEINALI², EHSAN AREFIAN¹, AMIR ATASHI^1,3, MAHIN NIKOUGOFTAR ZARIF⁴

BIOCELL, Vol.33, No.1, pp. 33-38, 2009, DOI:10.32604/biocell.2009.33.033

Abstract The kidney has an inherent ability for recovery and regeneration following acute damage. However, there has been much contention as to the source of regenerating renal cells. The aim of this study was to isolate and characterize these cells. Normal rat kidneys were minced and cells were isolated with collagenase I and were cultured in an expansion medium. Adherent cells were isolated and expanded for more than 120 days in vitro. These cells had the potential of trans-lineage differentiation into neural cells, adipocytes and osteocytes. These cells also expressed Nucleostemin, Cyclin D1, Notch1 and Survivin which More >

Torroba MC, HA Paccapelo, L Aguilera, J Mazzola

Phyton-International Journal of Experimental Botany, Vol.77, pp. 93-102, 2008, DOI:10.32604/phyton.2008.77.093

Abstract An optimized methodology for improving regeneration of maize plants by direct organogenesis was evaluated. Our objective was to embarobtain genetically homogeneous plants through in vitro methods to regenerate clumps of multiple shoots from shoot tips at high frequency. Cultures were initiated from shoot tips of experimental lines of maize on a Murashige & Skoog (MS) medium supplemented with 2 mg/l benziladenine. Two experimental forage maize lines were used, in which two frequencies of subcultures were evaluated, namely line L. 850 (every 15 or 30 days) and line L.769 (every 30 days). It was observed that the More >

J. B. Kim¹, P. Leucht^1,2, K. Lam¹, C. A. Luppen¹, D. ten Berg³, R. Nusse³, J. A. Helms¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 201-201, 2006, DOI:10.32604/mcb.2006.003.201

Abstract This article has no abstract. More >

C. N. Rios¹, J. N. Augustine¹, A. B. Mathur¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 173-174, 2006, DOI:10.32604/mcb.2006.003.173

Abstract This article has no abstract. More >

C. Niyibizi¹, F. Li¹, X. Wang¹, X. Liao¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 149-149, 2006, DOI:10.32604/mcb.2006.003.149

Abstract This article has no abstract. More >

L. Yang¹, K. L. Paul Sung¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 147-147, 2006, DOI:10.32604/mcb.2006.003.147

Abstract This article has no abstract. More >

A-J. Wang¹, Q. Ao^{1, 2}, K. Gong¹, Z-H. Zheng¹, G-Y. Lu¹, G. Wang¹, Q. He¹, L-J. Kong¹, Y-D. Gong¹, N-M. Zhao¹, X-F. Zhang¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 143-144, 2006, DOI:10.32604/mcb.2006.003.143

Abstract This article has no abstract. More >

A. C. PONTAROLI, E. L. CAMADRO

BIOCELL, Vol.29, No.3, pp. 313-317, 2005, DOI:10.32604/biocell.2005.29.313

Abstract Callus growth and plant regeneration from long-term callus cultures were studied in two elite clones of Asparagus officinalis cv. Argenteuil, to establish a suitable protocol for a prospective in vitro selection program. Callus initiation and growth was evaluated on MS medium with 3% sucrose, 0.9% agar, 1 mg.l^-1 kinetin, and three levels of 2,4-D. The highest callus relative growth was obtained on medium with 1.5 mg.l^-1 2,4-D and 1 mg.l^-1 kinetin. Shoot primordia (SP) induction from >18-months-old calluses was evaluated on several media; the highest percentage of SP induction (89%) and average number of SP per callus (8.6) were More >

CARLOS A. MARTÍN, JOSÉ MIGUEL SURUR, MARCELA N. GARCÍA, FÉLIX CORRONS, AMADO F. BADRÁN

BIOCELL, Vol.29, No.1, pp. 33-38, 2005, DOI:10.32604/biocell.2005.29.033

Abstract Variations in DNA synthesis (DNAs) and Nucleolar Organizer Regions (NORs) were studied in the littoral cell population from regenerating liver of C3HS inbred mice standardized for periodicity analysis. Immunohistochemical detection of Bromodeoxyuridine (BrdU) with a monoclonal antibody and silver staining of NORs (AgNORs) were assessed by means of a digital image analysis system in histological sections. Tissue samples were obtained every four hours from the 30th to the 54th hours after a partial hepatectomy. The results showed, in both parameters, a gradual increment of the values during the period studied, with highest values (DNAs 107.1 More >