Lilan Gao^1,2,*, Qingxian Yuan^1,2, Ruixin Li^3,*, Lei Chen^1,2, Chunqiu Zhang^1,2, Xizheng Zhang^1,2

Molecular & Cellular Biomechanics, Vol.15, No.2, pp. 85-98, 2018, DOI:10.3970/mcb.2018.00329

Abstract Silk fibroin-typeⅡcollagen scaffold was made by 3D printing technique and freeze-drying method, and its mechanical properties were studied by experiments and theoretical prediction. The results show that the three-dimensional silk fibroin-typeⅡ collagen scaffold has good porosity and water absorption, which is (89.3%+3.26%) and (824.09%+93.05%), respectively. With the given strain value, the stress of scaffold decreases rapidly firstly and then tends to be stable during the stress relaxation. Both initial and instantaneous stresses increase with increase of applied strain value. The creep strains of scaffold with different stress levels show the two stages: the rapidly increasing… More >

Flores S¹, A Promis², L Faúndez³

Phyton-International Journal of Experimental Botany, Vol.85, pp. 324-332, 2016, DOI:10.32604/phyton.2016.85.324

Abstract Jubaea chilensis (Molina) Baill. (Chilean palm) is an endemic species in Chile. It has been classified as a vulnerable species because of the reduction and the fragmentation of its population numbers, the exploitation of the palms for the production of syrup, the indiscriminate harvest of seed for human consumption, and the reduction of the accompanying native vegetation cover. The southernmost limit of its natural distribution is located in Palmas de Tapihue (Pencahue, Maule region). The objective of this study was to benchmark the conservation status of this population and its accompanying vegetation. The population of J. chilensis… More >

Li RS¹, YJ Tao², FJ Liu², X Hu², QL Xu², KY Li²

Phyton-International Journal of Experimental Botany, Vol.85, pp. 87-93, 2016, DOI:10.32604/phyton.2016.85.087

Abstract The grass pea (Lathyrus sativus L.) and flatpod peavine (Lathyrus cicera L.) are the most economically important and widely cultivated Lathyrus species. However, their utilization is limited due to the presence of their endogenous toxin β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP). Thus, a β-ODAP free variety should be developed through some plant breeding technique like either mutational breeding or genetic-manipulation. In this circumstance, the plant regeneration of Lathyrus species becomes a bottleneck. In the present study, an efficient system for in vitro regeneration of L. sativus with high β-ODAP levels, and L. cicera with low β-ODAP levels, was developed from different explants (axillary… More >

Pranav S. Sapkal^1*, Abhaykumar M. Kuthe¹, Divya Ganapathy², Shantanu C. Mathankar³, Sudhanshu Kuthe⁴

Molecular & Cellular Biomechanics, Vol.13, No.2, pp. 115-136, 2016, DOI:10.3970/mcb.2016.013.131

Abstract Biphasic calcium phosphate scaffolds with 20/80 HA/TCP ratio were fabricated using the 3D-Bioplotting system to heal critical size defects in rabbit tibia bone. Four different architectures were printed in a layer by layer fashion with lay down patterns viz. (a) 0°– 90°, (b) 0°– 45°– 90°– 135°, (c) 0°–108°– 216° and (d) 0°– 60°– 120°. After high-temperature sintering scaffolds were coated with collagen and were further characterized by (FTIR) Fourier Transform Infrared Spectroscopy, (SEM) Scanning Electron Microscopy, (XRD) X-Ray diffraction, Porosity analysis and Mechanical testing. Scaffold samples were tested for its ability to induce cytotoxicity… More >

Heng Zuo^*, Dalin Tang^*,†,‡, Chun Yang^*,§, Glenn Gaudette^¶, Kristen L. Billiar^¶, Pedro J. del Nidok^II

Molecular & Cellular Biomechanics, Vol.12, No.2, pp. 67-85, 2015, DOI:10.3970/mcb.2015.012.067

Abstract Right ventricular (RV) dysfunction is a common cause of heart failure in patients with congenital heart defects and often leads to impaired functional capacity and premature death. Myocardial tissue regeneration techniques are being developed for the potential that viable myocardium may be regenerated to replace scar tissues in the heart or used as patch material in heart surgery. 3D computational RV/LV/Patch models with fluid-structure interactions (FSI) were constructed based on data from a healthy dog heart to obtain local fluid dynamics and structural stress/strain information and identify optimal conditions under which tissue regeneration techniques could… More >

Qian J^1,2, Z Wang¹, Z Liu¹, W Kuang^1,2, CA Busso³

Phyton-International Journal of Experimental Botany, Vol.83, pp. 407-414, 2014, DOI:10.32604/phyton.2014.83.407

Abstract Understanding the changes in the total bud bank, and its contribution to community regeneration, in response to grassland enclosure to livestock grazing, is crucial for grassland management. Despite its importance, the contribution of the total bud bank and that of different bud types to community regeneration as a whole have been rarely explored. The vegetative offspring recruited from different bud types was investigated in grasslands having different enclosure durations to livestock grazing on a typical steppe of Inner Mongolia, China. Total vegetative offspring density was significantly higher (p<0.05) under continuous grazing than in fenced grasslands,… More >

Wilckens P¹, MP Fernández², M Gómez¹, I Peña³, G Montenegro¹

Phyton-International Journal of Experimental Botany, Vol.82, pp. 81-90, 2013, DOI:10.32604/phyton.2013.82.081

Abstract Buddleja globosa Hope is a well-known native, medicinal plant because of its digestive and cicatrizing properties. Due to the increasing domestic and international demand, B. globosa abundance and distribution in Chile has resulted in the intensive exploitation of the plant’s natural habitat, without consideration of the plant’s regenerative capacity. The main objective of this research was to establish the B. globosa growth cycle and model its regeneration cycle. This would allow for more productive, efficient and sustainable use and management of this species, thus ensuring its long-term conservation. We selected two study sites, one wild and another one… More >

Yongren Wu^∗, Sarah Cisewski^∗, Barton L. Sachs^†, Hai Yao^∗,†,‡

Molecular & Cellular Biomechanics, Vol.10, No.2, pp. 159-182, 2013, DOI:10.3970/mcb.2013.010.159

Abstract This study examines the effects of cartilage endplate (CEP) calcification and the injection of intervertebral disc (IVD) cells on the nutrition distributions inside the human IVD under physiological loading conditions using multiphasic finite element modeling. The human disc was modeled as an inhomogeneous mixture consisting of a charged elastic solid, water, ions (Na⁺ and Cl⁻), and nutrient solute(oxygen,glucose and lactate) phases. The effect of the endplate calcification was simulated by a reduction of the tissue porosity (i.e., water volume faction) from 0.60 to 0.48. The effect of cell injection was simulated by increasing the cell density… More >

Marco A.Velasco^∗, Diego A. Garzón-Alvarado^†

Molecular & Cellular Biomechanics, Vol.10, No.2, pp. 137-157, 2013, DOI:10.3970/mcb.2013.010.137

Abstract The design of porous scaffolds for tissue engineering requires methods to generate geometries in order to control the stiffness and the permeability of the implant among others characteristics. This article studied the potential of the reaction-diffusion systems to design porous scaffolds for bone regeneration. We simulate the degradation of the scaffold material and the formation of new bone tissue over canal-like, spherical and ellipsoid structures obtained by this approach. The simulations show that the degradation and growth rates are affected by the form of porous structures. The results have indicated that the proposed method has More >

O’Connor-Sánchez¹ A, AV Domínguez-May¹, MA Keb-Llanes¹, YJ Peña-Ramírez², VA Herrera-Valencia¹

Phyton-International Journal of Experimental Botany, Vol.79, pp. 25-29, 2010, DOI:10.32604/phyton.2010.79.025

Abstract A reliable protocol for plant regeneration of Mullein Nightshade (Solanum donianum Walp.) was developed from in vitro leaf explants. They were cultured on Murashige and Skoog semisolid medium, supplemented with several combinations of zeatin riboside and 1-naphthaleneacetic acid for shoot regeneration. The formulation that originated most shoots per explant was 8.5 μM zeatin riboside in absence of 1-naphthalene acetic acid. Explants with shoots were transferred to Murashige and Skoog medium, with half the normal salt concentration and without plant growth regulators for elongation. Elongated shoots were individualized, and they rooted readily in half-strength Murashige and Skoog More >