SITI NABILAH^1,2; TRI HANDOYO^2,3; KYUNG-MIN KIM⁴; MOHAMMAD UBAIDILLAH^2,*

BIOCELL, Vol.46, No.7, pp. 1633-1641, 2022, DOI:10.32604/biocell.2022.019111 - 17 March 2022

Abstract Somatic embryogenesis is an asexual reproduction process that occurs in many plant species, including rice. This process contains several totipotency markers such as Somatic Embryogenesis Receptor-like Kinase (SERK), Leafy Cotyledon1 (LEC1) and WUSCHEL-Related Homeobox4 (WOX4) and also a helpful model for embryo development and clones and transformations. Here, we report the gene expression during somatic embryo development correlates with regeneration frequency in 14 Javanica rice (pigmented and non-pigmented) using modifified N6 media supplemented with Kinetin (2.0 mg/L) and NAA (1.0 mg/L). Although there have been advances in understanding the genetic basis of somatic embryogenesis in… More >

C. Pabitha^*, B. Vanathi

Computer Systems Science and Engineering, Vol.42, No.3, pp. 949-961, 2022, DOI:10.32604/csse.2022.021086 - 08 February 2022

Abstract Skin is the largest body organ that is prone to the environment most specifically. Therefore the skin is susceptible to many damages, including burn damage. Burns can endanger life and are linked to high morbidity and mortality rates. Effective diagnosis with the help of accurate burn zone and wound depth evaluation is important for clinical efficacy. The following characteristics are associated with the skin burn wound, such as healing, infection, painand stress and keloid formation. Tissue regeneration also takes a significant amount of time for formation while considering skin healing after a burn injury. Deep… More >

YASHVI SHARMA¹, SHARDA RAY², SUJATA MOHANTY^1,*

BIOCELL, Vol.46, No.6, pp. 1435-1438, 2022, DOI:10.32604/biocell.2022.018612 - 07 February 2022

Abstract Neurodegenerative disorders are a vicious woe to the public health and wellness. Uncertainty in their underlying causes, lack of effective biomarkers for their early detection, existence of only supportive therapy, and their ever rising incidence creates an unmatched need for targeted therapies. Mesenchymal Stem Cells (MSCs) have found to be promising candidates for regenerative and remedial therapy in neurodegenerative disorders, however several biological risks and practical issues impede in their translational utility. Deriving from MSCs are certain Extracellular Vesicles (EVs), which aid in the paracrine action of MSCs and have lately gained the scientific interest More >

BENSHUAI YOU¹, HUI QIAN^1,2,*

BIOCELL, Vol.46, No.6, pp. 1459-1463, 2022, DOI:10.32604/biocell.2022.018378 - 07 February 2022

Abstract Exosomes, especially from mesenchymal stem cells, have attracted extensive attention in regeneration medicine. Mesenchymal stem cells derived exosomes (MSCs-exosomes) have shown anti-inflammatory, anti-oxidant, anti-apoptosis and tissue regeneration effects in a variety of tissue injury repair models. MSCs-exosomes hold many excellent properties such as low immunogenicity, biocompatibility, and targeting capability. With the in-depth study on the generation and function of exosomes, MSCs-exosomes are considered to be the bright stars in the field of regenerative medicine. However, there are still many obstacles to overcome in terms of exosomes isolation, clinical trials and safety evaluation. In this article, More >

KENJI TSUJI^*, SHINJI KITAMURA, JUN WADA

BIOCELL, Vol.46, No.4, pp. 899-902, 2022, DOI:10.32604/biocell.2022.018594 - 15 December 2021

Abstract Mesenchymal stem cells (MSCs) have abilities to mediate tissue protection through mechanisms of anti-apoptosis, anti-oxidative stress and anti-fibrosis as well as tissue regeneration through mechanisms of cell proliferation, differentiation and angiogenesis. These effects by MSCs are mediated by a variety of factors, including growth factors, cytokines and extracellular vesicles (EVs). Among these factors, EVs, containing proteins, mRNA and microRNAs (miRNA), may carry their contents into distant tissues with high stability. Therefore, the treatment with MSC-derived EVs may be promising as ‘natural’ drug delivery systems (DDS). Especially, the treatment of MSC-derived EVs with the manipulation of More >

PETRA KRAUS^1,*, ANKITA SAMANTA¹, SINA LUFKIN², THOMAS LUFKIN¹

BIOCELL, Vol.46, No.4, pp. 893-898, 2022, DOI:10.32604/biocell.2022.018432 - 15 December 2021

Abstract Pain and lifestyle changes are common consequences of intervertebral disc degeneration (IVDD) and affect a large part of the aging population. The stemness of cells is exploited in the field of regenerative medicine as key to treat degenerative diseases. Transplanted cells however often face delivery and survival challenges, especially in tissues with a naturally harsh microniche environment such as the intervertebral disc. Recent interest in the secretome of stem cells, especially cargo protected from microniche-related decay as frequently present in degenerating tissues, provides new means of rejuvenating ailing cells and tissues. Exosomes, a type of More >

Yajun Li^1,#, Yumeng Cui^1,#, Bingjie Wang¹, Yue Li¹, Mengmeng Zhang², Silan Dai¹, He Huang^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 869-888, 2022, DOI:10.32604/phyton.2022.018659 - 09 December 2021

Abstract Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum, which relies on an optimal regeneration and transformation system. However, the regeneration system of different chrysanthemum cultivars varies, and the regeneration time of most cultivars is long. To screen cultivars with highly efficient regeneration, leaves and shoot tip thin cell layers (tTCL) from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media (MS) supplemented with 1.0–5.0 mg L⁻¹ 6-benzylaminopurine (6-BA) and 0.1–1.0 mg L⁻¹ α-naphthaleneacetic (NAA). The results showed that the most efficient regeneration… More >

Mengtong Liu¹, Yangyang Zhou¹, Tongyu Liu¹, Jianyu Lu¹, Jing Qu², Shuyan Guan^2,*, Yiyong Ma^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 363-374, 2022, DOI:10.32604/phyton.2022.016325 - 26 September 2021

Abstract Immature embryos of inbred maize (Zea mays) lines (H8183, H8184, and H8185) were used for Agrobacterium infection. We used the β-glucuronidase gene (GUS) as the target gene and the glufosinate resistance gene (bar) as the selection marker. We conducted research on several aspects, such as different genotypes, coculture conditions, screening agent concentrations, and concentrations of indole-3-butytric acid (IBA), 6-benzylaminopurine (6-BA), and ascorbic acid (Vc) in the differentiation medium. We optimized the genetic transformation system, and the obtained results indicated that among the three lines studied, the induction rate of H8185 was the highest at 93.2%, followed by H8184, More >

Shidan Chi¹, Tao Luan^1,*, Yan Liang², Xundong Hu², Yan Gao^3,*

FDMP-Fluid Dynamics & Materials Processing, Vol.17, No.1, pp. 71-80, 2021, DOI:10.32604/fdmp.2021.013178 - 09 February 2021

Abstract Improving the primary steam parameters is one of the most direct ways to improve the cycle efficiency of a power generation system. In the present study, the typical problem connected to the excessively high superheat degree of extraction steam in an ultra-supercritical (USC) double-reheat unit is considered. Using a 1000 MW power plant as an example, two systems (case 1 and case 2) are proposed, both working in combination with a regenerative steam turbine. The thermal performances of these two systems are compared with that of the original system through a heat balance method and More >

Si ran Wang^#, Jia Yan^#, Bu er Ha, Yu’e Bai^*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 595-604, 2021, DOI:10.32604/phyton.2021.013693 - 07 February 2021

Abstract Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering technology has been successfully used in many plant species, but it is limited in Hydrangea. Here we established an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL⁻¹ 6-BA and 0.5 mgL⁻¹ 1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots More >