Yajun Li^1,#, Yumeng Cui^1,#, Bingjie Wang¹, Yue Li¹, Mengmeng Zhang², Silan Dai¹, He Huang^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 869-888, 2022, DOI:10.32604/phyton.2022.018659

Abstract Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum, which relies on an optimal regeneration and transformation system. However, the regeneration system of different chrysanthemum cultivars varies, and the regeneration time of most cultivars is long. To screen cultivars with highly efficient regeneration, leaves and shoot tip thin cell layers (tTCL) from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media (MS) supplemented with 1.0–5.0 mg L⁻¹ 6-benzylaminopurine (6-BA) and 0.1–1.0 mg L⁻¹ α-naphthaleneacetic (NAA). The results showed that the most efficient regeneration media were MS +… More >

Mengtong Liu¹, Yangyang Zhou¹, Tongyu Liu¹, Jianyu Lu¹, Jing Qu², Shuyan Guan^2,*, Yiyong Ma^2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.2, pp. 363-374, 2022, DOI:10.32604/phyton.2022.016325

Abstract Immature embryos of inbred maize (Zea mays) lines (H8183, H8184, and H8185) were used for Agrobacterium infection. We used the β-glucuronidase gene (GUS) as the target gene and the glufosinate resistance gene (bar) as the selection marker. We conducted research on several aspects, such as different genotypes, coculture conditions, screening agent concentrations, and concentrations of indole-3-butytric acid (IBA), 6-benzylaminopurine (6-BA), and ascorbic acid (Vc) in the differentiation medium. We optimized the genetic transformation system, and the obtained results indicated that among the three lines studied, the induction rate of H8185 was the highest at 93.2%, followed by H8184, with H8183… More >

Shidan Chi¹, Tao Luan^1,*, Yan Liang², Xundong Hu², Yan Gao^3,*

FDMP-Fluid Dynamics & Materials Processing, Vol.17, No.1, pp. 71-80, 2021, DOI:10.32604/fdmp.2021.013178

Abstract Improving the primary steam parameters is one of the most direct ways to improve the cycle efficiency of a power generation system. In the present study, the typical problem connected to the excessively high superheat degree of extraction steam in an ultra-supercritical (USC) double-reheat unit is considered. Using a 1000 MW power plant as an example, two systems (case 1 and case 2) are proposed, both working in combination with a regenerative steam turbine. The thermal performances of these two systems are compared with that of the original system through a heat balance method and an exergy balance strategy. The… More >

Si ran Wang^#, Jia Yan^#, Bu er Ha, Yu’e Bai^*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 595-604, 2021, DOI:10.32604/phyton.2021.013693

Abstract Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering technology has been successfully used in many plant species, but it is limited in Hydrangea. Here we established an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL⁻¹ 6-BA and 0.5 mgL⁻¹ 1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots proliferation coefficient… More >

Jiaji Zhang¹, Xiaofei Long¹, Yuhao Weng¹, Tielong Cheng², Jisen Shi¹, Jinhui Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 571-582, 2021, DOI:10.32604/phyton.2021.014191

Abstract Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study, we successfully developed a large-scale micropropagation protocol for C. japonicum for the first time. Sterilized shoots were excised and used as explants for shoot induction on several basal media, supplemented with different concentrations of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N⁶ -Benzyladenine (6-benzylaminopurine) (BA), α-naphthaleneacetic acid (NAA) and Gibberellic acid (GA₃). After comparison, the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L^–1 BA, 0.05 mg L^–1 NAA and… More >

YUNZE XUAN^1,#, BIN JIN^1,#, SAYAN DEB DUTTA^5,#, MENGMENG LIU², ZAIXIAN SHEN¹, XIWEN LIU³, YANG KANGJUAN^4,*, LIM KI-TAEK^5,*

BIOCELL, Vol.44, No.3, pp. 389-400, 2020, DOI:10.32604/biocell.2020.09170

Abstract Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth, leading to the destruction of tooth-supporting tissues and loss of alveolar bone. Suberoylanilide hydroxamic acid (SAHA), a class of histone deacetylase (HDAC) inhibitor, has the potential to stimulate osteoblast differentiation by acetylating histone proteins, and thus suppressing the expression of adipogenic and chondrogenic genes. However, the effect of SAHA on the differentiation of human periodontal ligament stem cells (hPDLSCs) is yet to be elucidated. Herein, we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs… More >

Peng Jiao^1,#, Ruiqi Ma^2,#, Zhuo Qi¹, Zhenzhong Jiang¹, Siyan Liu¹, Jing Qu³, Shuyan Guan^1,*, Yiyong Ma^3,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 121-130, 2020, DOI:10.32604/phyton.2020.07980

Abstract This research uses the immature embryos of inbred maize lines (GSH9901, Hi01, Hi02, and Chang 7-2) as receptor materials to establish the callus induction system. These inbred lines provide the receptor materials for the genetic regeneration of maize and the verification of the genetic functions of maize. The factor experiment and orthogonal experiments were used to investigate the impacts of different genotypes, immature embryo size, shield orientation, 2, 4-D concentration, proline concentration, and folic acid concentration on the induction rate of embryogenic callus tissue. A sensitivity experiment testing glyphosate (Bar) and an antibiotic (Cefotaxime sodium) were also conducted. The results… More >

Xu Wang^1,3, Jianhong Zhou^1,2, Bo Pang^1,2, Dawei Zhao^1,2,*

Journal of Renewable Materials, Vol.7, No.12, pp. 1363-1380, 2019, DOI:10.32604/jrm.2019.08218

Abstract Introduction of the strategy of anhydrous calcium carbonate protection incorporated with the drop by drop reaction, high-purity 1-butyl-3-methylimidazolium chloride ([Bmim] Cl) was prepared at reaction temperature of 80°C for only 10 h. Cellulose samples from different biomass sources (with different degree of polymerization characteristic) could be rapidly (no more than 10 minutes) and completely dissolved in the [Bmim] Cl using a microwave-assisted ionothermal route. Homogeneous cellulosic regenerates with high degree of polymerization and thermal stability characteristics were obtained through a coagulation process in water. Furthermore, the dissolved celluloses were readily regenerated into solid products such as casting films and spinning… More >

MARÍA LAURA FONTANA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY*

BIOCELL, Vol.33, No.3, pp. 179-186, 2009, DOI:10.32604/biocell.2009.33.179

Abstract With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of thidiazuron in… More >

YOUSOF GHEISARI^1,2, MASOUD SOLEIMANI^3*, SIROUS ZEINALI², EHSAN AREFIAN¹, AMIR ATASHI^1,3, MAHIN NIKOUGOFTAR ZARIF⁴

BIOCELL, Vol.33, No.1, pp. 33-38, 2009, DOI:10.32604/biocell.2009.33.033

Abstract The kidney has an inherent ability for recovery and regeneration following acute damage. However, there has been much contention as to the source of regenerating renal cells. The aim of this study was to isolate and characterize these cells. Normal rat kidneys were minced and cells were isolated with collagenase I and were cultured in an expansion medium. Adherent cells were isolated and expanded for more than 120 days in vitro. These cells had the potential of trans-lineage differentiation into neural cells, adipocytes and osteocytes. These cells also expressed Nucleostemin, Cyclin D1, Notch1 and Survivin which are commonly expressed in… More >