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  • Open Access

    PROCEEDINGS

    Segment Crack Formation and Density Regulation in Air Plasma Sprayed Coatings

    Liuyu Yang1, Peng Jiang1, Tiejun Wang1,*

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.25, No.3, pp. 1-1, 2023, DOI:10.32604/icces.2023.010538

    Abstract Air Plasma Sprayed (APS) Thermal Barrier Coatings (TBCs) have been widely used in land-based gas engines for enhancing the high temperature performance due to their outstanding thermal insulation and high durability. Introducing the segment cracks into APS-TBCs to enhance its durability has been quite attractive approaches nowadays. Qualitative conclusions have been drawn to explore the mechanisms on segment crack formation in the past decades. This article acts as a quantitative study of segment crack formation and crack density regulation mechanism in APS Yttria-Stabilized Zirconia (YSZ) TBCs with experimental observations and analytical calculations. An in-situ stress measurement method is developed through… More >

  • Open Access

    ARTICLE

    Genome-Wide Analysis of the F3′5′H Gene Family in Blueberry (Vaccinium corymbosum L.) Provides Insights into the Regulation of Anthocyanin Biosynthesis

    Xiaolan Guo1,2, Jinbin Hu3, Shimei Yang4, Delu Wang2,*, Jianbing Wang1

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2683-2697, 2023, DOI:10.32604/phyton.2023.029820

    Abstract The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis, abiotic stress, and hormone signaling. In this study, 14 F3′5′H genes were identified from the blueberry genome. The chromosomal distribution, physicochemical properties, F3′5′H domain, conserved motifs, cis-acting elements, and intron/exon compositions were analyzed. The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites. The results of qRT-PCR showed that VcF3′5′Hs (especially VcF3′5′H4) were highly expressed at the ripening stage. Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum. Co-expression analysis showed that the VcF3′5′H gene family… More >

  • Open Access

    ARTICLE

    Transcriptome Profiling of the Salt-Stress Response in Paper Mulberry

    Jie Zhang1, Yingwei Zhao2, Hongying Li3, Jianwei Ni4,*, Dongmei Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2591-2610, 2023, DOI:10.32604/phyton.2023.028338

    Abstract Paper mulberry is a high-quality woody feed resource plant with high crude protein content. It is widely distributed in China and has excellent characteristics of salt and alkali tolerance. Paper mulberry has ecological and economic importance. Salt stress has become a critical factor with the increasing degree of soil salinity that restricts plant growth. In the saline-alkali environments, transcriptome expression is altered leading to phenotypic defects in most plants. However, the regulatory mechanism related to paper mulberry’s salt-stress (SS) response is not clearly understood. In the present study de novo transcriptomic assembly was performed, and gene expression levels were measured… More >

  • Open Access

    ARTICLE

    Genotypic Divergence, Photosynthetic Efficiency, Sodium Extrusion, and Osmoprotectant Regulation Conferred Salt Tolerance in Sorghum

    Ashaduzzaman Sagar1 , Md. Sabibul Haque1, Md. Alamgir Hossain1, Md. Nesar Uddin1, Jannat E. Tajkia1, Md. Ashik Mia1, Toufika Hossain Shabi2, Md. Solaiman Ali Fakir1, Md. Abdul Kader3,4,5, Walid Soufan6, Md. Atikur Rahman7, Muhammad Aamir Iqbal8, Mohammad Sohidul Islam9, Ayman El Sabagh10,*, A. K. M. Zakir Hossain1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2349-2368, 2023, DOI:10.32604/phyton.2023.028974

    Abstract Salt stress is one of the major limitations to modern agriculture that negatively influences plant growth and productivity. Salt tolerant cultivar can provide excellent solution to enhance stress tolerance with plant fitness to unfavorable environments. Therefore, this study was aimed to screen salt tolerant sorghum genotypes through evaluating of different morphological, biochemical, and physiological attributes in response to salinity stress. In this study, we have been evaluated total six sorghum genotypes including Hybrid sorgo, Debgiri, BD-703, BD-706, BD-707, and BD-725 under salt stress (12 dS m−1 NaCl). The response variables included length and weight of root and shoot, root: shoot… More >

  • Open Access

    REVIEW

    Molecular regulation mechanism of oocyte maturation in beef cattle

    BINWU BAO, JINPENG WANG, YANXIA LI, FEN FENG, ZHONGXIANG JI, ZHUOMA LUORENG*, XINGPING WANG*

    BIOCELL, Vol.47, No.7, pp. 1509-1518, 2023, DOI:10.32604/biocell.2023.028646

    Abstract Bovine oocytes are one of the indispensable cells in cattle reproduction and have become a research hot spot in cattle reproduction in recent years. The maturation process of oocytes is mainly regulated by enzymes, hormones, cytokines, and other molecules. The factors affecting cattle oocyte maturation have been previously studied to clarify the molecular mechanisms of cattle oocyte maturation. In this review article, phospholipid protein-3-kinase/protein kinase B, mitogen-activated protein kinase/extracellular signal-regulated kinase, Janus kinase/signal transducer and activator of transcription, epidermal growth factor receptor/extracellular signal-regulated kinase, and other signaling pathways related to oocyte maturation are discussed. In addition, the molecular mechanisms of… More >

  • Open Access

    ARTICLE

    Hepatitis B virus X protein-mediated upregulation of miR-221 activates the CXCL12-CXCR4 axis to promote NKT cells in HBV-related hepatocellular carcinoma

    YUE CAO, LIN HU, YISHU TANG*

    BIOCELL, Vol.47, No.7, pp. 1537-1548, 2023, DOI:10.32604/biocell.2023.027205

    Abstract Backgrounds: Both hepatitis B virus X protein (HBx) and microRNA-221 (miR-221) have been implicated in the development of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). The present study demonstrates that HBx promotes HCC cell proliferation via the C-X-C motif chemokine ligand 12-C-X-C chemokine receptor type 4 (CXCL12-CXCR4) axis. We predict that HBx/miR-221-mediated CXCL12/CXCR4 signaling induces NKT cells to promote HBV-related HCC. Methods: After miR-221 mimic, miR-221 mimic negative control, miR-221 inhibitor, miR-221 inhibitor negative control were transfected into cells, the expression of CXCL12 and miR-221 was detected by qPCR and western blot. Then we constructed a stable HBV-HCC cell line.… More >

  • Open Access

    ARTICLE

    Urban Drainage Network Scheduling Strategy Based on Dynamic Regulation: Optimization Model and Theoretical Research

    Xiaoming Fei*

    Intelligent Automation & Soft Computing, Vol.37, No.2, pp. 1293-1309, 2023, DOI:10.32604/iasc.2023.038607

    Abstract With the acceleration of urbanization in China, the discharge of domestic sewage and industrial wastewater is increasing, and accidents of sewage spilling out and polluting the environment occur from time to time. Problems such as imperfect facilities and backward control methods are common in the urban drainage network systems in China. Efficient drainage not only strengthens infrastructure such as rain and sewage diversion, pollution source monitoring, transportation, drainage and storage but also urgently needs technical means to monitor and optimize production and operation. Aiming at the optimal control of single-stage pumping stations and the coordinated control between two-stage pumping stations,… More >

  • Open Access

    REVIEW

    Protein-mediated interactions in the dynamic regulation of acute inflammation

    RYAN STARK*

    BIOCELL, Vol.47, No.6, pp. 1191-1198, 2023, DOI:10.32604/biocell.2023.027838

    Abstract Protein-mediated interactions are the fundamental mechanism through which cells regulate health and disease. These interactions require physical contact between proteins and their respective targets of interest. These targets include not only other proteins but also nucleic acids and other important molecules as well. These proteins are often involved in multibody complexes that work dynamically to regulate cellular health and function. Various techniques have been adapted to study these important interactions, such as affinity-based assays, mass spectrometry, and fluorescent detection. The application of these techniques has led to a greater understanding of how protein interactions are responsible for both the instigation… More >

  • Open Access

    ARTICLE

    Transcriptome Analysis of Auxin Drives Cone Size Regulation in Fokienia hodginsii

    Shunde Su1,2,#, Huan Chang3,#, Xiangyang Kang1,*, Renhua Zheng2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1713-1723, 2023, DOI:10.32604/phyton.2023.027586

    Abstract As the reproductive organ of the endangered species Fokienia hodginsii, the size of the cones is a constraint on the reproductive renewal of the population. In this study, the molecular basis of the influence of cone size on F. hodginsii was elucidated by comparing the phenotype, biochemistry, and transcriptome of two cultivars of F. hodginsii (‘FJ431’ and ‘FJ415’). The two cultivars differed significantly in cone size, with FJ431 having a significantly larger cone size and weight than FJ415, 1.32 and 1.90 times that of FJ415, respectively. RNA-Seq analysis of both cultivars retrieved 75,940 genes whose approximate functions were classified as… More >

  • Open Access

    ARTICLE

    Tanshinone IIA protects intestinal epithelial cells from ferroptosis through the upregulation of GPX4 and SLC7A11

    HAN WANG1,2,#, YANG SUN1,2,#, XIAOXU ZHANG2,3, XIAOYING WANG4, YUJUN XIA1,*, LISHENG WANG1,2,*

    BIOCELL, Vol.47, No.5, pp. 1107-1115, 2023, DOI:10.32604/biocell.2023.027131

    Abstract Background: Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract. The destruction of the intestinal epithelial barrier is one of the major pathological processes in IBD pathology. Growing evidence indicated that epithelial cell ferroptosis is linked to IBD and is considered a target process. Methods: RAS-selective lethal 3 (RSL3) was used to induce ferroptosis in intestinal epithelial cell line No. 6 (IEC-6) cells, and cell ferroptosis and the effects of tanshinone IIA (Tan IIA) were determined by cell counting kit-8 (CCK-8), reactive oxygen species (ROS) staining, Giemsa staining and transmission electron microscope (TEM). The cell viability… More >

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