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  • Open Access

    ARTICLE

    Brief Note : Proacrosin-acrosin activity in capacitated and acrosome reacted sperm from cryopreserved bovine semen

    M. I. ROSATTI, M. T. BECONI, M. CÓRDOBA

    BIOCELL, Vol.28, No.3, pp. 311-316, 2004, DOI:10.32604/biocell.2004.28.311

    Abstract Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acrosin at pH 8. Capacitation with heparin and quercitin failed to induce conversion of proacrosin to acrosin. An increase in acrosin activity produced by the presence of progesterone, in a dose-dependent manner, was related with the induction of true acrosome reaction. The total level of acrosin activity registered showed… More >

  • Open Access

    ARTICLE

    The actin filament network associated to Sertoli cell ectoplasmic specializations

    JUAN CARLOS CAVICCHIA1*, MABEL FÓSCOLO1 , JORGE IBAÑEZ1, CHRISTOPHER LILLIG2, FRANCISCO CAPANI3

    BIOCELL, Vol.35, No.3, pp. 81-90, 2011, DOI:10.32604/biocell.2011.35.081

    Abstract Junctional devices in Sertoli cells conform the blood-testis barrier and play a key role in maturation and differentiation of germ cells. The spacial distribution of ectoplasmic specializations of Sertoli cells was studied by β-actin immunolabelling, using laser confocal and transmission electron microscopy. For confocal microscopy, β-actin immunolabelling of ectoplasmic specializations was studied over the background of either prosaposin or glutaredoxin immunolabelling of the Sertoli cytoplasm. Labelling was found near the basal lamina, surrounding early spermatocytes (presumably in leptotene-zygotene) or at one of two levels in the seminiferous epithelium: (1) around deep infoldings of the Sertoli cell cytoplasm, in tubular stages… More >

  • Open Access

    ARTICLE

    Aboveground biomass and concentration of nutrients in semiarid rangeland plant species: Influence of grazing and soil moisture

    Gul B1, M Islam2, S Ahmad3, S Gul1

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 94-99, 2016, DOI:10.32604/phyton.2016.85.094

    Abstract Spatial and temporal patterns of aboveground biomass and nutritive value of rangeland species with respect to the influence of grazing and soil moisture were investigated. The research was conducted during two years at the Tomagh Research Station, near Sanjawi, Ziarat District, Balochistan, Pakistan. This area is protected from grazing since 1998; however, some of the area is open for grazing. Three sites were selected for research purposes: a protected plain, a protected hilly, and an unprotected plain grazed area. Sampling was carried out during the spring, summer and autumn seasons. Results revealed that soil moisture was greater at the protected… More >

  • Open Access

    ARTICLE

    Effects of grazing on plant species diversity and carbon partitioning in semiarid rangelands of northeastern China

    Hu FL1,2, B Liu1,3, ZM Liu1,3, YT Fang1,3, CA Busso4

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 209-221, 2015, DOI:10.32604/phyton.2015.84.209

    Abstract Grasslands are one of the most widespread landscapes worldwide, covering approximately one-fifth of the world’s land surface, where grazing is a common practice. How carbon storage responds to grazing in steppes remains poorly understood. We quantified the effects of grazing on community composition and species diversity, and carbon storage in two typical grasslands of northeastern China, one in Horqin and the other one in Hulunbeier. In both grasslands, grazing did not influence plant species diversity. However, it substantially decreased aboveground carbon by 31% and 54% in Horqin and Hulunbeier, respectively. Fenced and grazing treatments showed a similar belowground carbon at… More >

  • Open Access

    ARTICLE

    Influence of woody species on aerial growth of perennial grasses in semi-arid rangelands of central Argentina

    Blazquez FR1,2,3, DV Peláez1,3,4, RJ Andrioli1, OR Elia1,2,3

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 397-405, 2014, DOI:10.32604/phyton.2014.83.397

    Abstract The interaction between woody and grass species in semi-arid rangelands of central Argentina was assessed through the evaluation of the impact of woody species on grass growth over a 2-y period. The study comprised two morpho-physiologically different woody species and two cool-season perennial grass species highly preferred by domestic livestock. The study involved the assessment of total green length (TGL) and number of green leaves (NGL) per tiller in grasses growing beneath the canopy of woody species and in open rangeland areas. Soil temperature, pH and water, nitrogen, phosphorus and organic matter content were estimated at the same study sites.… More >

  • Open Access

    ARTICLE

    Water stress response of Tricepiro and its progenitors Triticale and Trigopiro

    Ruiz MA1,3, A Golberg1,2, ML Molas1

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 263-274, 2014, DOI:10.32604/phyton.2014.83.263

    Abstract In semiarid regions rainfall is often scarce and erratic in space and time, making valuable the exploration of news genotypes with superior behavior in dry land systems. Tricepiro (X Triticosecale Wittmack x X Agrotriticum Ciferri & Giacom) is a synthetic cereal with long cycle and high biomass production in semiarid environments. However its performance under controlled water stress has not yet been evaluated. The aim of this work was to investigate the effect of water stress on tricepiro genotypes in comparison with their progenitors: triticale and trigopiro. Morphophysiological traits related to forage yield were evaluated in the greenhouse and the… More >

  • Open Access

    ARTICLE

    Effect of tillage system on bread wheat yield and grain quality in the temperate semiarid central zone of La Pampa

    Miravalles MT1, MN Fioretti1, H Mirassón2, ML Faraldo2, L Möck1, RE Brevedan1

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 263-273, 2013, DOI:10.32604/phyton.2013.82.263

    Abstract In the present study the effects of three tillage systems: conventional (CT), vertical (VT) and direct drilling (DD) on three bread wheat varieties of different cycle length, were studied, for yield and yield components. Their influence on grain quality, expressed through thousand kernel weight (TKW, g), test weight (TW, kg/hL), grain protein content (GPC, %) and yellowberry percentage (YBP, %) were also studied. The highest average yield was under CT and DD, while the lowest was under VT. The lowest dry matter accumulation and one third fewer spikes per unit area were determined under VT. Concerning grain quality, a comparatively… More >

  • Open Access

    ARTICLE

    First report of Tobacco mosaic virus (TMV) and Poinsettia mosaic virus (PnMV) in poinsettia (Euphorbia pulcherrima Willd. Ex Klotzch) in Mexico

    Ocampo Ocampo T1, DL Ochoa Martínez1, S Ramírez Rojas2, G Valdovinos Ponce1, C Nava Díaz1

    Phyton-International Journal of Experimental Botany, Vol.82, pp. 235-241, 2013, DOI:10.32604/phyton.2013.82.235

    Abstract The Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), in Zacatepec Morelos, started a genetic improvement program of wild and semi-cultivated poinsettia plants ("nochebuena de sol"). It is important to generate a phytosanitary database that allows the release of new varieties and hybrids that provide tolerance to the major pathogens reported in cultivated poinsettia plants. The phytosanitary status of the “nochebuena de sol” plants is not available in Mexico, so the objective of this research was to generate preliminary data about viruses associated to these materials. Based on DAS-ELISA and RT-PCR results, we reported the presence of the Tobacco… More >

  • Open Access

    ARTICLE

    Mnesithea selloana: morphology of sinflorescence, maturity and caryopsis quality

    Galussi AA1,2, ME Moya1,2, LR Zimmermann1, FG Marchese1,2, YG Gillij1,2, F Torres1,2

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 255-260, 2012, DOI:10.32604/phyton.2012.81.255

    Abstract Mnesithea selloana (known as cola de largarto, teyú ruguay) is a spring-summer-fall species that flowers and fructifies in that period; it has good forage quality, and it is in danger of extinction in the natural grasslands at the study region. The objectives of this work were to study the sinflorescence morphology, and the maturity and quality of the caryopsis to know its reproductive aspects in relation to the dissemination of this especies in grazing areas. The sinflorescence was studied in two plants during a period of 45 days, between October and December 2010, within an experimental field of the UNER… More >

  • Open Access

    ARTICLE

    Responses of Conocarpus lancifolius to environmental stress: a case study in the semi-arid land of Kuwait

    Redha A, P Suleman, R Al-Hasan, M Afzal

    Phyton-International Journal of Experimental Botany, Vol.81, pp. 181-190, 2012, DOI:10.32604/phyton.2012.81.181

    Abstract Cococarpus lancifolius Engl. grows wild in Djibouti, Somalia and East Africa, and South Asia. The species was introduced into Kuwait, where it is exposed to several stresses: oil pollution, salinity, high temperature and harsh climatic conditions. These conditions are prevalent in its semi-arid environment. In this study, the impact of salinity (2, 6 and 10%), temperature (20, 30 and 40 °C), polyethylene glycol (10, 30 and 60%) and drought has been investigated on C. lancifolius. Proline (Pro) accumulation (5.5 ± 0.03 µg/g dry weight; p≤0.05-0.005), protein (16.47 ± 0.052 mg/g; p≤0.001), free amino acids (10.92 ± 0.082 mg/g; p≤0.006) and… More >

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