Guan Z-J^1,2,*, B Guo^1,*, Y-L Huo³, J-K Dai⁴, Y-H Wei¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 51-58, 2012, DOI:10.32604/phyton.2012.81.051

Abstract The initiation and development of organogenic buds and somatic embryos in HBsAg-transgenic cherry tomato (Lycopersicum esculentum var. cerasiforme) mutant were studied histologically. The leaf explants of the mutant were cultured on Murashige & Skoog (MS) basal medium supplemented with 6-BA 1.0 mg/L and IAA 0.05 mg/L for callus induction. Histological studies on the leaf explants of the mutant at various developmental stages revealed that organogenic buds first appeared in the axillary position of explants on the 14th cultured day, and then somatic embryos formed in the same mutant explants after 35 days of culture. Transmission electron microscopy and scanning electron… More >

Pérez Castañeda LM, MA Cruz Hernández, A Mendoza Herrera

Phyton-International Journal of Experimental Botany, Vol.80, pp. 27-34, 2011, DOI:10.32604/phyton.2011.80.027

Abstract Various studies have shown the symbiotic association of Azospirillum brasilense with different grasses of nutritional importance. A high variability among the isolated strains has been observed. This is consistent with the genetic diversity that Azospirillum has shown in sugarcane isolates. However, if this is also the case in maize and sorghum isolates is unknown. In addition, it has also been reported that most of the isolates come from tropical regions where water is not limiting for maize and sorghum production. We analyzed the genetic variability of Azospirillum brasilense isolates obtained from arid zones of Northeast Mexico in maize and sorghum.… More >

Sacristán M, AM Millanes, B Fontaniella, ME Legaz, C Vicente

Phyton-International Journal of Experimental Botany, Vol.77, pp. 253-262, 2008, DOI:10.32604/phyton.2008.77.253

Abstract Secreted arginases from Evernia prunastri and Xanthoria parietina thalli, exhibiting lectin activity, have been purified to homogeneity. Analyses of both amino acid and glycoside composition are reported. Purified proteins have been subjected to tryptic digestion and some amino acid sequences have been analyzed. An undecapeptide from Evernia arginase, which shows some degree of homology with the domain to which Mn²⁺ binds, a heptapeptide and an undecapeptide from Xanthoria arginase have been analyzed, and homologies with some arginases from other fungi and plants have been found. More >

Giménez¹ C, E de García², O Haddad³

Phyton-International Journal of Experimental Botany, Vol.77, pp. 65-79, 2008, DOI:10.32604/phyton.2008.77.065

Abstract Evaluation of clonal micropropagation and phenotype stability of elite somaclones are critical steps for development of new varieties. In the present work somaclon variant CIEN BTA-03 (resistant to Black Sigatoka), obtained through in vitro process from cultivar Williams (susceptible to Black Sigatoka), was micropropagated via apical shoot culture for five multiplication cycles in 0.5 mg/l of benzyl-aminopurine (BA). To verify the genetic stability of the progeny of this elite material, random amplified polymorphic DNA (RAPD) markers were used. A total of 5,292 monomorphic bands were obtained from the amplification of fifty six DNA samples (extracted from vitroplants randomly selected) with… More >

Menéndez-Yuffá¹ A, L Ríos-Bolívar²

Phyton-International Journal of Experimental Botany, Vol.77, pp. 49-64, 2008, DOI:10.32604/phyton.2008.77.049

Abstract Regeneration and multiplication of coffee (Coffea arabica L.) through somatic embryogenesis can be efficiently achieved through different methods. However, it is very important to analyze the progenies obtained by these methods in order to establish their genetic stability. The objectives of this research were (1) to determine the protein electrophoretic patterns in leaves of coffee vitroplants which were regenerated through somatic embryogenesis, and (2) to compare these patterns with those of the explant donor plants. The protein patterns of some coffee genotypes were initially determined to detect possible differences among them. Each analyzed genotype showed a characteristic set of protein… More >

Luján-Rangel¹ R, E Olivares-Sáenz², RE Vázquez-Alvarado², L Garza-Ocañas³, O Torres-Alanís³

Phyton-International Journal of Experimental Botany, Vol.76, pp. 61-77, 2007, DOI:10.32604/phyton.2007.76.061

Abstract Karwinskia parvifolia (Kp) and Karwinskia humboldtiana (Kh) are poisonous plants of the rhamnacea family. Toxicological and clinical studies performed with one of the compounds present in the fruit [peroxisomicine A1 (PA1)] suggest its possible pharmacological usefulness in the treatment of certain types of cancer. This is why these plants are cultivated: to increase their productivity of PA1. We studied the effects of nitrogen and species on seed viability. The germination curve was determined for Kp with (Kp-WN) and without (Kp-NN) nitrogen, and for Kh without nitrogen (Kh-NN) using the probit model. This is characterized by the probability of germination in… More >

M. R. Hematiyan^1,*, M. Arezou¹, N. Koochak Dezfouli¹, M. Khoshroo¹

CMES-Computer Modeling in Engineering & Sciences, Vol.121, No.2, pp. 661-686, 2019, DOI:10.32604/cmes.2019.08275

Abstract In this paper, some remarks for more efficient analysis of two-dimensional elastostatic problems using the method of fundamental solutions are made. First, the effects of the distance between pseudo and main boundaries on the solution are investigated and by a numerical study a lower bound for the distance of each source point to the main boundary is suggested. In some cases, the resulting system of equations becomes ill-conditioned for which, the truncated singular value decomposition with a criterion based on the accuracy of the imposition of boundary conditions is used. Moreover, a procedure for normalizing the shear modulus is presented… More >

Pingping Ma¹, Wanqian Liu^1,*, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 95-95, 2019, DOI:10.32604/mcb.2019.07363

Abstract Globally, consumption of sodium (5.8 g per day) was far above the optimal levels (2.3 g per day). High intake of sodium was the leading dietary risk factor for deaths, which caused by cardiovascular disease [1]. Nevertheless, how high-salt intake leads to the occurrence of many cardiovascular diseases such as atherosclerosis is still not very clear. Dmitrieva has reported that elevated sodium concentration promoted thrombogenesis by activating the signal pathway of NFAT5 (nuclear factor of activated T cells 5), a transcription factor which orchestrates cellular defense against osmotic stress [2]. Inflammatory is accompanied with the entire development process of atherosclerosis.… More >

Ying Fang¹, Jianhua Wu^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.1, pp. 5-6, 2019, DOI:10.32604/mcb.2019.05695

Abstract For hemostasis and thrombosis, some proteins, such as Von Willebrand Factor (VWF, a multimeric plasma glycoprotein synthesized in endothelial cells and megakaryocytes and secreted to circulation or attached to endothelial cells), the metalloprotease ADAMTS13 (a disintegrin and metalloprotease with a thrombospondin type 1 motif, member 13), P-selectin (one of three selectin family members with a N-terminal C-type lectin domain, an epidermal growth factor (EGF)-like module, a series of consensus repeat (CR) units, a transmembrane segment and a short cyto-plasmic domain) and β2 integrin. In adhesion and aggregation of circulating platelets towards to the sites of vascular injury, VWF on vascular… More >

MANOOCHEHR MESSRIPOUR¹, AZADEH MESRIPOUR²

BIOCELL, Vol.37, No.2, pp. 17-21, 2013, DOI:10.32604/biocell.2013.37.017

Abstract Tyrosine hydroxylase and tryptophan hydroxylase are key rate limiting enzymes in the biosynthesis of dopamine and serotonin, respectively. Since both enzymes are active in striatum, and affected by age, this study was undertaken to investigate interaction between dopamine and serotonin synthesis in brain striatal synaptosomes of aging rat. Male Wistar rats (3 and 30 month old) were killed by decapitation and brain striatal synaptosomes were prepared by discontinuous Ficoll/sucrose gradient technique. Synaptosomes were incubated in the presence of added pargiline (monoamineoxidase inhibitor), dopamine or serotonin synthesized during 25 min was measured by HPLC, employing electrochemical detection. Dopamine synthesis in synaptosomes… More >