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  • Open Access

    REVIEW

    Review : Reticulocyte maturation: mitoptosis and exosome release

    CHARLES GÉMINARD, AUDE DE GASSART, MICHEL VIDAL

    BIOCELL, Vol.26, No.2, pp. 205-215, 2002, DOI:10.32604/biocell.2002.26.205

    Abstract During the differentiation of erythroid cells, a vast program of maturation takes place, leading to decay or elimination of organelles, including the nucleus, mitochondria, ribosomes, lysosomes, endoplasmic reticulum and Golgi apparatus. During the last step of red cell maturation, remaining organelles, primarily mitochondria and ribosomes but also vestiges of others are finally cleared from the cell. This cleaning session also affects specific proteins that are partially or entirely removed from the cell surface. The interplay of the various events and their causal relationships are approached here. More >

  • Open Access

    ARTICLE

    Somatic embryogenesis induction from immature embryos of Sorghum bicolor L. (Moench)

    Espinoza-Sánchez EA1, YA Sánchez-Peña2, JA Torres-Castillo3, EA García-Zambrano2, JE Treviño Ramírez2, F Zavala-García2, SR Sinagawa-García2

    Phyton-International Journal of Experimental Botany, Vol.87, pp. 105-112, 2018, DOI:10.32604/phyton.2018.87.105

    Abstract In this study, we established an in vitro regeneration system from immature embryos of four varieties of Sweet Sorghum with biotechnological potential. A total of 17 treatments were assesses in the induction of somatic embryogenic calli. We analyzed the effect of Murashige y Skoog media supplemented with different concentrations of plant growth regulators such as auxins and cytokinins. The callus induction response was evaluated using three immature embryos development stages and based on the number of embryos with callus induction. Different responses were observed in the embryos depending of the embryo age, culture condition, media composition and variety. The results… More >

  • Open Access

    ARTICLE

    Nitric oxide synthase activity in tissues of the blowfly Chrysomya megacephala (Fabricius, 1794)

    A. C. FARALDO1, A. SÁ-NUNES2, L. H. FACCIOLI2, E. A. DEL BEL3, E. LELLO4

    BIOCELL, Vol.31, No.2, pp. 205-211, 2007, DOI:10.32604/biocell.2007.31.205

    Abstract Although insects lack the adaptive immune response of the mammalians, they manifest effective innate immune responses, which include both cellular and humoral components. Cellular responses are mediated by hemocytes, and humoral responses include the activation of proteolytic cascades that initiate many events, including NO production. In mammals, nitric oxide synthases (NOSs) are also present in the endothelium, the brain, the adrenal glands, and the platelets. Studies on the distribution of NO-producing systems in invertebrates have revealed functional similarities between NOS in this group and vertebrates. We attempted to localize NOS activity in tissues of naïve (UIL), yeast-injected (YIL), and saline-injected… More >

  • Open Access

    ARTICLE

    Kaempferol and docetaxel diminish side population and down-regulate some cancer stem cell markers in breast cancer cell line MCF-7

    SARA SOLTANIAN1, HELIA RIAHIRAD1, ATHAREH PABARJA1, MOHAMMAD REZA KARIMZADEH2, KOLSOUM SAEIDI3,4

    BIOCELL, Vol.41, No.2-3, pp. 33-40, 2017, DOI:10.32604/biocell.2017.41.033

    Abstract Cancer stem cells (CSCs) are a small subpopulation of cancer cells whose resistance to chemotherapy and radiotherapy plays a pivotal role in treatment failure, tumor recurrence and metastasis. Today, the ability of many phytochemicals in targeting of CSCs has been identified. Kaempferol is a plant phytoestrogen that was recognized as a useful agent for targeting cancer cells by apoptosis induction, cell cycle arrest and inhibition of angiogenesis, migration, and invasion of cancer cells. In this study, we compared the effect of docetaxel as a common breast cancer chemotherapy drug and kaempferol on MCF-7 breast CSCs. Results showed that both docetaxel… More >

  • Open Access

    ARTICLE

    Some Factors That May Determine the Frequency Response of Cells and Tissue to Applied Electrical and Mechanical Forces

    F. X. Hart1

    Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 235-235, 2006, DOI:10.32604/mcb.2006.003.235

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    Structural and Antioxidative Stabilities of Liposomal Nanocapsulsin Membrane Physicochemical Properties

    H. Nakagawa1, T. Shiina2, M. Kotani3, H. Kotani1, S. Ueno1

    Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 163-163, 2006, DOI:10.32604/mcb.2006.003.163

    Abstract This article has no abstract. More >

  • Open Access

    ARTICLE

    Trypanosoma cruzi invasion in non-phagocytic cells: an ultrastructural study

    Juan Agustín CUETO3, Emile SANTOS BARRIAS6, Wanderley de SOUZA4, 5, Patricia Silvia ROMANO1, 2

    BIOCELL, Vol.42, No.3, pp. 105-108, 2018, DOI:10.32604/biocell.2018.07017

    Abstract Trypanosoma cruzi is the causative agent of Chagas disease. This parasite requires the intracellular niche in order to proliferate and disseminate the infection. After invasion, T. cruzi resides temporarily in an acidic vacuole which is lysed by a not well-understood mechanism. Transmission electron microscopy was used to describe the process of T. cruzi escape from the parasitophorous vacuole over the time. Using HeLa (non-professional phagocytic cells) as host cell, we observed that recently internalized parasites reside in a membrane-bounded vacuole. A few hours later, the first sign of vacuole disruption appeared as membrane discontinuities. This observation was followed by a… More >

  • Open Access

    ARTICLE

    Study of phylogenic relationships of some wild and crop species of Iranian Crocus by ITS nuclear loci

    Farshid GOLSHANI1, Barat Ali FAKHERI1, Mahmood SOLOUKI1, Nafiseh MAHDINEZHAD1, Majid Reza KIANI FERIZ2

    BIOCELL, Vol.43, No.3, pp. 225-232, 2019, DOI:10.32604/biocell.2019.07547

    Abstract ITS (Internal Transcription Spacer of nuclear ribosomal DNA) regions method was applied to identify and study the phylogenetic relationships existing between 41 individuals of Crocus, including 8 species collected in Iran (4 crop and 8 wild individuals) and 16 species (2 crop and 27 wild individuals) already registered in GenBank (NCBI). PCR amplifications were performed using the primer designed on the nucleotide sequence of nuclear region (ITS). A total of 12 sequences were obtained and registered in NCBI database. In particular, 4 of these sequences were not already present in the scientific library. Nucleotide polymorphic sites were counted for ITS… More >

  • Open Access

    ARTICLE

    Trypanosoma rangeli: growth in mammalian cells in vitro and action of a repositioned drug (17-AAG) and a natural extract (Artemisia sp. essential oil)

    Ana Laura CIMADOR1,#, Emeli Luciana GALANTE1,#, Lucila Ibel MUÑOZ1,#, Patricia Silvia ROMANO2,3, Antonella Denisse LOSINNO1,2, María Cristina VANRELL1,2,3

    BIOCELL, Vol.43, No.1, pp. 13-20, 2019, DOI:10.32604/biocell.2019.07018

    Abstract Trypanosoma rangeli and T. cruzi are both parasitic unicellular species that infect humans. Unlike T. cruzi, the causative agent of Chagas disease, T. rangeli is an infective and non-pathogenic parasite for humans, but pathogenic for vectors from the Rhodnius genus. Because both species can coexist in different hosts and overlap their infective cycles but very little is known about the infection of T. rangeli in mammalian cells, we decided to characterize both the development of this parasite in cell culture and the effect of therapeutic agents with potential trypanocidal action on it. We found that T. rangeli exhibits a cycle… More >

  • Open Access

    ABSTRACT

    Assessment of Some Atomization Models Used in Spray Calculations

    M.S. Raju & Dan Bulzan

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.19, No.4, pp. 109-110, 2011, DOI:10.3970/icces.2011.019.109

    Abstract The paper presents the results from a validation study undertaken as a part of the NASA's fundamental aeronautics initiative on high altitude emissions in order to assess the accuracy of several atomization models used in both sub-cooled and superheat spray calculations. As a part of this investigation we have undertaken the validation based on four different cases to investigate the spray charactereristics of (1) a ashing jet generated by the sudden release of pressurized R134A from cylindrical nozzle, (2) a liquid jet atomizing in a subsonic cross ow, (3) a Parker-Hannin pressure swirl atomizer, and (4) a single-element LDI (Lean… More >

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