MARÍA ESTHER DIUPOTEX-CHONG¹, NÉSTOR J. CAZZANIGA², ALEJANDRA HERNÁNDEZ-SANTOYO³, JOSÉ MIGUEL BETANCOURT-RULE⁴

BIOCELL, Vol.28, No.3, pp. 279-285, 2004, DOI:10.32604/biocell.2004.28.279

Abstract Mitotic chromosomes of the freshwater snail Pomacea patula catemacensis (Baker 1922) were analyzed on gill tissue of specimens from the type locality (Lake Catemaco, Mexico). The diploid number of chromosomes is 2n = 26, including nine metacentric and four submetacentric pairs; therefore, the fundamental number is FN = 52. No sex chromosomes could be identified. The same chromosome number and morphology were already reported for P. flagellata, i.e., the other species of the genus living in Mexico. The basic haploid number for family Ampullariidae was reported to be n° =°14 in the literature; so, its reduction to n° =°13 is… More >

PAOLA TALIA¹ , EDUARDO J. GREIZERSTEIN¹ , H. ESTEBAN HOPP^1,2 , NORMA PANIEGO¹ , LIDIA POGGIO2 AND RUTH A. HEINZ^1,2*

BIOCELL, Vol.35, No.1, pp. 19-28, 2011, DOI:10.32604/biocell.2011.35.019

Abstract Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. This selection was based on previously identified QTL associated to S. sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization, and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG 16… More >

Wang QL, DH Liu, JY Yue

Phyton-International Journal of Experimental Botany, Vol.85, pp. 155-161, 2016, DOI:10.32604/phyton.2016.85.155

Abstract Allium cepa var. agrogarum L. seedlings are sensitive to Cd stress. We used fluorescence imaging to indicate that Cd2+ was localized in cytoplasm in the epidermis of the basal parts of root and vascular tissues after Cd treatment. The nucleoli and the cell walls were the first storage sites of Cd²⁺. When Cd exposure was prolonged, severe irregularly-shaped nuclei were induced. We used silver nitrate staining to analyze the effects of different concentrations (1–300 μM) of cadmium chloride on chromosome, nucleolus and nucleolus organizer regions (NORs) in root tip cells. Cd²⁺ induced c-mitosis, chromosome bridges, chromosome stickiness and micronuclei. More… More >

Guan Z-J^1,3,4*, S-B Lu^2*,Y-L Huo⁵, B Guo³, Z-P Guan⁶, Y-H Wei³

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 351-357, 2015, DOI:10.32604/phyton.2015.84.351

Abstract Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of the mutant increased steadily until… More >

Turkyilmaz Unal B¹, A Esiz Dereboylu²

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 144-147, 2015, DOI:10.32604/phyton.2015.84.144

Abstract The aim of the study was to investigate some physiological effects of an insecticide (Akira 20-SP) with acetamiprid agent on Cin misir Adapop 10 and Sert misir Adapop 2 cultivars of corn plants in different concentrations [0 g/L, 0.6 g/L (recommended concentration), 1.2 g/L]. Photosynthetic pigment substances, total soluble protein and proline contents were determined. Analysis of treated leaves showed that the recommended concentration resulted in higher chlorophyll value in Cin misir Adapop 10 and Sert misir Adapop 2. Carotenoid amounts increased in Cin misir Adapop 10 and decreased in Sert misir Adapop 2 to the control. Total protein contents… More >

Hernández-Quintero JD¹, MH Reyes-Valdés², DV Mendoza-Rodríguez³, M Gómez-Martínez², R Rodríguez-Herrera⁴

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 107-112, 2015, DOI:10.32604/phyton.2015.84.107

Abstract The genus Dasylirion is a group of plants typically present in the Chihuahuan Desert, perennial, with a dioecious sexual behavior and commonly called sotoles. This genus has been little studied from the biological point of view, and the bases of its reproductive response remain unknown. In this work we studied the chromosome number and meiotic response of Dasylirion cedrosanum in the county of Saltillo, Coahuila, located at the North East of Mexico. For the preparation of mitotic chromosomes, we used a technique based on enzymatic treatment with pectolyase and cellulase, as well as staining with acetocarmin dye. For the study… More >

Wang QL, LT Zhang, JH Zou, DH Liu, JY Yue

Phyton-International Journal of Experimental Botany, Vol.83, pp. 291-298, 2014, DOI:10.32604/phyton.2014.83.291

Abstract The effects of various cadmium (Cd) concentrations (10, 50 and 100 μM) on root growth, cell division and micronuclei formation in root tip cells of Allium cepa var. agrogarum L. were investigated to better understand the processes of Cd-induced apoptosis. The results indicated that 10 μM of Cd had an obviously passive influence on root growth during the 24 h treatment, and that the influence was even more serious with increasing Cd concentrations and duration of treatments. The mitotic index decreased with increasing Cd concentration and duration of treatments. Cadmium induced c-mitosis, chromosome bridges, chromosome stickiness and micronuclei. The frequency… More >

Luján-Rangel R¹, E Olivares-Sáenz², RE Vázquez-Alvarado², L Garza-Ocañas¹, O Torres-Alanís^1(†), HJ Garza-Ulloa¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 247-253, 2012, DOI:10.32604/phyton.2012.81.247

Abstract Karwinskia parvifolia Rose synthesizes a toxin (PA1), which might be used to treat some types of tumors. Variability from 16 plants of K. parvifolia and the effect of nitrogen was evaluated on its dry fruit yield. The experiment was carried out using a crossover design, two treatment sequences and two production cycles. Collected data were: dry fruit yield (DFY), dry fruit weight (DFW) and dry endocarp weight (DWE) per plant. During Cycle 1, the group of plants that received additional nitrogen (CN) produced higher yields than the group that received no additional nitrogen (SN). Ratios of CN/SN were 1.68 (DFY),… More >

Simonetti E¹, E Alba², A Delibes²

Phyton-International Journal of Experimental Botany, Vol.81, pp. 139-142, 2012, DOI:10.32604/phyton.2012.81.139

Abstract In a previous work, deduced amino acid sequences from twenty wheat peroxidase genes were assigned to seven groups designated as TaPrx108 to TaPrx114. Some of these apoplastic peroxidases have previously shown to play different roles in the plant defense responses to infection by the cereal cyst nematode Heterodera avenae. In the present study, PCR marker analysis using Sears’s aneuploid wheat lines cv. ‘Chinese Spring’ was used to locate four genes encoding peroxidase isozymes. The TaPrx111-A, TaPrx112-D and TaPrx113-F genes were located on the short arm of chromosome 2B and the TaPrx109-C on the long arm of chromosome 1B. These results… More >

Velázquez-Sepúlveda I, MC Orozco-Mosqueda, CM Prieto-Barajas, G Santoyo

Phyton-International Journal of Experimental Botany, Vol.81, pp. 81-87, 2012, DOI:10.32604/phyton.2012.81.081

Abstract Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they corresponded to… More >