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  • Open Access

    ARTICLE

    Antimicrobial evaluation of extracts obtained from tropical micromycetes against phytopathogens

    Moreno Pérez P1,3, M Gamboa Angulo1, G Heredia2, B Canto Canché1, CM Rodríguez García1, IL Medina Baizabal1, L Peraza Echeverría1

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 7-14, 2016, DOI:10.32604/phyton.2016.85.007

    Abstract Nowadays, it is highly necessary to find more and safer agrochemicals. In this sense, micromycetes are an important source of natural products which could be used to control plant diseases. Therefore with the aims to contribute in this searching of natural products with antimicrobial applications, a total of 49 fungal strains were isolated from the Yucatan Peninsula. These fungi were cultured in fermented rice, their respective organic extracts macerated in ethyl acetate (EAE) and methanol (ME) were obtained and tested against five fungal pathogens of agricultural importance. These included Alternaria chrysanthemi, Colletotrichum gloeosporioides, Mycosphaerella fijiensis, and the bacteria Erwinia carotovoraMore >

  • Open Access

    ARTICLE

    Spatial distribution of Asclepias curassavica L., in the State of Mexico, Mexico

    Ramírez-Dávila JF1, RA Jiménez-Carrillo2, JR Sánchez-Pale1, M Rubí Arriaga1, DK Figueroa-Figueroa1

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 193-202, 2014, DOI:10.32604/phyton.2014.83.193

    Abstract This study contributes to the knowledge of the biodiversity of the flora of the State of Mexico through an analysis of the distribution and abundance of Asclepias curassavica L., which is planted with ornamental and medicinal potential. The study was carried out in the municipalities of Temascaltepec, Malinalco and Valle de Bravo. The spatial statistical method was used to determine the distribution of A. curassavica based on the method SADIE (spatial analysis by remote indexes). Sampling transects of 200 m, marked every 10 m, were used in the three municipalities, during three times of the year, spring, summer and winter.… More >

  • Open Access

    ARTICLE

    High passage numbers induce resistance to apoptosis in C2C12 muscle cells

    LUCIA PRONSATO, ANABELA LA COLLA, ANA CAROLINA RONDA, LORENA MILANESI, RICARDO BOLAND, ANDREA VASCONSUELO*

    BIOCELL, Vol.37, No.1, pp. 1-9, 2013, DOI:10.32604/biocell.2013.37.001

    Abstract Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2 O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2 O2 induction of apoptosis. We propose serially cultured C2C12… More >

  • Open Access

    ARTICLE

    Cytotoxicity assessment of a gold nanoparticle-chitosan nanocomposite as an effi cient support for cell immobilization: comparison with chitosan hydrogel and chitosan-gelatin

    Mohammad Reza RAMEZANI1, Hossein NADERI-MANESH1, *, Hossain-Ali RAFIEEPOUR2

    BIOCELL, Vol.38, No.1, pp. 11-16, 2014, DOI:10.32604/biocell.2014.38.011

    Abstract Cell-based biosensors have become a research hotspot in the biosensors and bioelectronics fields. The main feature of cell-based biosensors is immobilization of living cells on the surface of transducers. Different types of polymers which are used as scaffolds for cell growth should be biocompatible and should have reactive functional groups for further attachment of biomolecules. In this work, cell attachment and proliferation on chitosan hydrogel, chitosan-gelatin and gold nanoparticle-chitosan nanocomposite membranes was studied. Characterization of the membranes was performed using scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). Cytotoxicity assessment on HEK293 cells was carried out for all… More >

  • Open Access

    ARTICLE

    Evidence for maternal transmission of a putative endosymbiont in the digestive gland of Pomacea canaliculata (Architaenioglossa, Ampullariidae)

    EDUARDO KOCH1,2, ISRAEL A. VEGA1,3,4, ALFREDO CASTRO-VAZQUEZ1,3,4

    BIOCELL, Vol.41, No.2-3, pp. 59-62, 2017, DOI:10.32604/biocell.2017.41.059

    Abstract The digestive gland of the apple snail Pomacea canaliculata lodges two types of pigmented corpuscles (identified as C and K corpuscles) which has been proposed as endosymbiont/s. Both corpuscular types are always present in the digestive gland of adult snails, they are released into the tubuloacinar lumen and are later expelled in the feces. On their part, hatchlings lack any C or K corpuscles in the digestive gland as well as in their feces, whereas C corpuscles appear in both the gland and feces within one week after hatching. Hence, it is possible that the detritivorous hatchlings acquire the putative… More >

  • Open Access

    ARTICLE

    Dynamic Pressures on Tunnel Roofs due to Vehicle Passages

    James D. Barnes1, Ethan R. Brush1, Mark S. Newmark1, Eric E. Ungar1, *

    Sound & Vibration, Vol.52, No.4, pp. 6-8, 2018, DOI:10.32604/sv.2018.03737

    Abstract Pressure and proximity measurements made in a tunnel indicate that a typical vehicle passage produced on the tunnel roof an initial pressure increase of small magnitude, followed by a sharp and more substantial drop in pressure below atmospheric. The magnitude of the pressure drop was found to increase with smaller clearances between the vehicle top and the tunnel roof, consistent with the Bernoulli relation and the vehicle speed. The dynamic pressures potentially may have significant effects on the vibration and noise environments on the lower floors of “air rights construction” buildings that span highways. More >

  • Open Access

    ARTICLE

    A Stochastic Analysis of a Brownian Ratchet Model for Actin-Based Motility

    Hong Qian1

    Molecular & Cellular Biomechanics, Vol.1, No.4, pp. 267-278, 2004, DOI:10.3970/mcb.2004.001.267

    Abstract In recent single-particle tracking (SPT) measurements on Listeria monocytogenes motility in cells [Kuo and McGrath (2000)], the actin-based stochastic dynamics of the bacterium movement has been analyzed statistically in terms of the mean-square displacement (MSD) of the trajectory. We present a stochastic analysis of a simplified polymerization Brownian ratchet (BR) model in which motions are limited by the bacterium movement. Analytical results are obtained and statistical data analyses are investigated. It is shown that the MSD of the stochastic bacterium movement is a monotonic quadratic function while the MSD for detrended trajectories is linear. Both the short-time relaxation and the… More >

  • Open Access

    ARTICLE

    Effect of Eugenia winzerlingii Extracts on Bemisia tabaci and Evaluation of its Nursery Propagation

    A. Cruz-Estrada1,2, E. Ruiz-Sánchez2, I.L. Medina Baizabal1, E. Balam-Uc1 and M. Gamboa-Angulo1,*

    Phyton-International Journal of Experimental Botany, Vol.88, No.2, pp. 161-170, 2019, DOI:10.32604/phyton.2019.05809

    Abstract The development of plant-derived products to control Bemisia tabaci Genn. (Hemiptera: Aleyrodidae) is an urgent need for production of horticultural crops. Plant extracts and essential oils of several species of the genus Eugenia (Myrtaceae) have shown insecticidal activity. In southern Mexico, leaf extracts from Eugenia winzerlingii showed nematicidal effect but its insecticidal properties have not been explored. Therefore, the objective of this study was to evaluate the insecticidal effect of aqueous and organic extracts from E. winzerlingii leaves on B. tabaci egg, nymph and adult stages, and else to explore its nursery propagation. Then, extracts of this species were obtained… More >

  • Open Access

    ARTICLE

    Prenatal exposure to the fluoride containing psychiatric drug fluoxetine and anti-oxidative alterations in the neonatal rat brain

    Ramesa Shafi BHAT1, Amina El GEZEERY2, Abir Ben BACHAN1, Mona Awad ALONAZI1, Leena Saleh ALSUHAIBANI1, Afaf El-ANSARY3,4

    BIOCELL, Vol.43, No.2, pp. 65-71, 2019, DOI:10.32604/biocell.2019.07012

    Abstract Fluoride is a key ingredient of many psychiatric drugs like fluoxetine (Prozac®, Fluoxetine®). Pregnant women frequently use this drug as they suffer from depression and anxiety disorders during this period. Fluoxetine is able to reach the fetus through the placenta and passes to the newborn through milk. In the present study, female Wistar rats were treated with 5, 10, and 20 mg/L fluoxetine (containing 94% fluorides) from pregnancy day 10 to day 20. After delivery, the levels of the enzymatic antioxidants in the brain of their offspring at postnatal day 2 were measured. The results showed that, in all fluoxetine… More >

  • Open Access

    ABSTRACT

    Immunoassay of Human Immunoglobulin Using Quartz Crystal Microbalance

    Sheng D. Chao

    The International Conference on Computational & Experimental Engineering and Sciences, Vol.17, No.2, pp. 33-34, 2011, DOI:10.3970/icces.2011.017.033

    Abstract We performed an immunoassay analysis using the Quartz Crystal Microbalance (QCM) biosensor to detect the specific binding reaction of the (Human IgG1)-(Anti-Human IgG1) protein pair. Both experimental and computational were used to study biomolecular binding reactions in microfluidic channels. We discussed the unsteady convective diffusion in the transportation tube and found that the distribution of the analyte concentration in the tube is strongly affected by the flow field. Due to this, large discrepancies between the simulations and experimental results were observed. We show that the conventional assumption of the uniform and steady analyte concentration used in the computational procedure is… More >

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