Min Dong1,#, Zongchang Song2,#, Xiaohui Lu1,3,#, Minxue Lu4,*, Chen Zhong1,*
Oncology Research, Vol.34, No.3, 2026, DOI:10.32604/or.2026.068508
- 24 February 2026
Abstract Background: Gastric cancer (GC) remains highly lethal, with metabolic reprogramming as a key hallmark. This study explores Centromere Protein F (CENPF)’s role in GC pathogenesis, specifically its regulation of glutamine metabolism. Methods: The Cancer Genome Atlas–Stomach Adenocarcinoma (TCGA-STAD), GSE19826, and GSE27342 datasets were analyzed by bioinformatics to identify key candidate genes in GC. The function of CENPF was assessed by flow cytometry, colony formation assays, and Cell Counting Kit-8 (CCK-8). RNA sequencing, metabolic profiling, chromatin immunoprecipitation (ChIP), western blot (WB), and luciferase reporter assay were employed to investigate the fundamental mechanisms. Results: CENPF was upregulated in GC… More >