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  • Open Access


    In vitro Antibacterial Activity of Moringa oleifera Ethanolic Extract against Tomato Phytopathogenic Bacteria

    Roberto Arredondo-Valdés1, Francisco D. Hernández-Castillo2, Mario Rocandio-Rodríguez1, Julia C. Anguiano-Cabello3, Madai Rosas-Mejía1, Venancio Vanoye-Eligio1, Salvador Ordaz-Silva4, Imelda V. López-Sánchez4, Laura D. Carrazco-Peña4, Julio C. Chacón-Hernández1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 895-906, 2021, DOI:10.32604/phyton.2021.014301

    Abstract The tomato (Solanum lycopersicum L.) is one of the world’s most important vegetable crops. Still, phytopathogenic bacteria affect the yield and quality of tomato cultivation, like Agrobacterium tumefeciens (At), Clavibacter michiganensis subsp. michiganensis (Cmm), Pseudomonas syringae pv. tomato (Pst), Ralstonia solanacearum (Rs), and Xanthomonas axonopodis (Xa). Synthetic chemical products are used mostly on disease plant control, but overuse generates resistance to bacterial control. This study aimed to evaluate the in vitro antibacterial activity of the ethanolic extract of Moringa oleifera Lam. leaves against At, Cmm, Pst, Rs, and Xa, as well as information about this plant species’ chemical composition. Antibacterial activity against pathogens observed by microplate technique, phytochemical screening, More >

  • Open Access


    Rapid delivery of Cas9 gene into the tomato cv. ‘Heinz 1706’ through an optimized Agrobacterium-mediated transformation procedure


    BIOCELL, Vol.45, No.1, pp. 199-215, 2021, DOI:10.32604/biocell.2021.012353

    Abstract Solanum lycopersicum ‘Heinz 1706’ is a pioneer model cultivar for tomato research, whose whole genome sequence valuable for genomics studies is available. Nevertheless, a genetic transformation procedure for this cultivar has not yet been reported. Meanwhile, various genome editing technologies such as transfection of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) ribonucleoprotein complexes into cells are in the limelight. Utilizing the Cas9-expressing genotype possessing a reference genome can simplify the verification of an off-target effect, resolve the economic cost of Cas9 endonuclease preparation, and avoid the complex assembly process together with single-guide RNA (sgRNA) in… More >

  • Open Access


    Image-Based Automatic Diagnostic System for Tomato Plants Using Deep Learning

    Shaheen Khatoon1,*, Md Maruf Hasan1, Amna Asif1, Majed Alshmari1, Yun-Kiam Yap2

    CMC-Computers, Materials & Continua, Vol.67, No.1, pp. 595-612, 2021, DOI:10.32604/cmc.2021.014580

    Abstract Tomato production is affected by various threats, including pests, pathogens, and nutritional deficiencies during its growth process. If control is not timely, these threats affect the plant-growth, fruit-yield, or even loss of the entire crop, which is a key danger to farmers’ livelihood and food security. Traditional plant disease diagnosis methods heavily rely on plant pathologists that incur high processing time and huge cost. Rapid and cost-effective methods are essential for timely detection and early intervention of basic food threats to ensure food security and reduce substantial economic loss. Recent developments in Artificial Intelligence (AI)… More >

  • Open Access


    Identification and analysis of AP2/ERF gene family in tomato under abiotic stress


    BIOCELL, Vol.44, No.4, pp. 777-803, 2020, DOI:10.32604/biocell.2020.010153

    Abstract AP2/ERE-type transcription factors, as a type of plant-specific transcription factors, play a key role in plant biotic and abiotic stress. Meanwhile, they have been studied in many plants, but rarely in tomatoes. In this study, we performed a genome-wide analysis of the SlAP2/ERF gene family of tomato, and finally identified 29 SlAP2/ERF genes and divided them into different subfamilies. At the same time, its basic physical and chemical properties were analyzed. We also constructed phylogenetic trees with 30 Arabidopsis AP2/ERF proteins and 28 potatoes AP2/ERF proteins to ensure conservative homology between them. In addition, we mapped… More >

  • Open Access


    Spatial Distribution of Nymphs Populations Bactericera cockerelli Sulc in Tomato Crops (Physalis ixocarpa Brot)

    Roberto Rivera-Martínez1, Agustín David Acosta-Guadarrama1, José Francisco Ramírez-Dávila2,*, Fidel Lara Vazquez1, Dulce Karen Figueroa Figueroa1

    Phyton-International Journal of Experimental Botany, Vol.88, No.4, pp. 449-458, 2019, DOI:10.32604/phyton.2019.06350

    Abstract Tomato crops (Physalis ixocarpa Brot.) are produced in almost all Mexico, part of the United States and Central America. Recently the tomato production has suffered economic losses of 70% to 80% due the presence of yellowing and floral abortion, whose causal agent has been attributed to the presence of phytoplasma; an insect vector of these phytoplasma is Bactericera cockerrelli Sulc. Alternative control of this psyllid has lacked effectiveness because their spatial distribution is unknown within tomato plots. This study aimed to determine the spatial distribution of populations of nymphs of B. cockerelli in four tomato plots, the determination… More >

  • Open Access


    Polymer-based encapsulation of Bacillus subtilis and its effect on Meloidogyne incognita in tomato

    Pacheco-Aguirre J, E Ruiz-Sánchez, A Reyes-Ramírez, J Cristóbal-Alejo, J Tun-Suárez, L Borges-Gómez

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 1-6, 2016, DOI:10.32604/phyton.2016.85.001

    Abstract Antagonistic bacteria used as biological control agent may loss effectiveness at the field due to environmental factors such as UV radiation, dryness and high temperature. An inexpensive alternative to protect antagonistic bacteria against such factors is the use of microencapsulating agents. In this work, the effect of microencapsulation of Bacillus subtilis with commercial gums on their antagonistic capacity against Meloidogyne incognita was evaluated. The efficiency of the microencapsulation was verified by the difference between the initial and final concentrations of protein release. The effectiveness as antagonist was evaluated against M. incognita in tomato under greenhouse More >

  • Open Access


    Phytochemical quality of tomato Saladette produced with organic substrates under greenhouse conditions

    González B ML1, M Fortis H1, P Preciado R1, MA Segura C1, E Salazar Sosa1, JL García H2, JR Esparza R3

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 71-78, 2016, DOI:10.32604/phyton.2016.85.071

    Abstract The aim of the current study was to evaluate yield and phytochemical quality of tomato fruits produced using organic substrates under greenhouse conditions. The applied treatments (organic substrates) were: T1 (80% River sand + 20% Vermicompost; T2 (90% River sand + 10% Compost); T3 Steiner Solution (80% River sand + 20% Perlite); T4 (80% River sand + 5% Soil + 15% Vermicompost); T5 (85% River sand + 15% Treated manure), and T6 (80% River sand + 5% Soil + 15% Treated manure). We used a totally randomized experimental design, with four replicate treatments. Evaluated variables… More >

  • Open Access


    Physiological and biochemical changes during organogenesis and somatic embryogenesis of HBsAg-transgenic cherry tomato mutant

    Guan Z-J1,3,4*, S-B Lu2*,Y-L Huo5, B Guo3, Z-P Guan6, Y-H Wei3

    Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 351-357, 2015, DOI:10.32604/phyton.2015.84.351

    Abstract Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of… More >

  • Open Access


    Meiotic analysis of four cross-pollinated generations in a synthetic autotetraploid population of husk tomato (Physalis ixocarpa)

    Rodríguez de León JI1, MH Reyes-Valdés2, DV Mendoza-Rodríguez3, F Ramírez-Godina2, V Robledo-Torres2, M Gómez-Martínez2, G Hernández-Guzmán4

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 101-106, 2015, DOI:10.32604/phyton.2015.84.101

    Abstract The cultivated husk tomato (Physalis ixocarpa) (2n = 2x = 24) is native from Mexico and Central America and shows a wide genetic variation. Presently, it is the fourth horticultural crop in cultivation surface in Mexico. The working team of this research previously developed an autotetraploid population by using colchicine. The objectives of the present work were to analyze the ploidy level and meiotic behavior of the subsequent generations (C3, C4, C5, C6) from the original (C2) composed only by plants with the duplicated genome from the Rendidora cultivar, and to determine pollen viability. As a… More >

  • Open Access


    Microbiological quality of tomato (Solanum lycopersicum L.) produced under greenhouse conditions in five Municipalities of the State of Mexico

    Ocaña-de Jesús RL, AT Gutiérrez-Ibáñez, JR Sánchez-Pale, MD Mariezcurrena-Berasain, G Velázquez-Garduño, A Laguna Cerda, I Rojas Puebla

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 45-50, 2015, DOI:10.32604/phyton.2015.84.045

    Abstract The aim of the current research was to determine tomato (Solanum lycopersicum L.) microbiological quality produced under greenhouse conditions in 5 municipalities of the State of Mexico. Studies were conducted during the 2013 production cycle to know the risks and apply prevention strategies prior to its consumption. A microbiological analysis of samples of irrigation water, soil and 100 tomato fruits variety cid was performed to determine Aerobic Mesophiles, Total Coliforms and Fecal Coliforms. The methodology used were those according to the Official Mexican Standards NOM- 109-SSA1-1994, NOM-110-SSA1-1994, NOM-092-SSA1-1994, NOM-113-SSA1-1994, and the Regulations of the National French More >

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