Zhenzhen SHI, Jiaxin CAO, Hailong PANG, Lingyun JIA, Kun SUN, Hanqing FENG^*

BIOCELL, Vol.44, No.1, pp. 89-99, 2020, DOI:10.32604/biocell.2020.08059

Abstract Cadmium (Cd) is one of the most widespread and toxic heavy metals to plants. Extracellular ATP (exATP) is thought to be an extracellular effector in regulating the physiological responses of plant cells to environmental stresses. However, the function of exATP in Cd-stressed plant cells is much unknown. The present work showed that treating tobacco (Nicotiana tabacum L. cv. Bright Yellow-2) cell-suspension cultures with exogenous CdCl₂ reduced the cell viability, exATP level, and Mg content. However, the production of reactive oxygen species (ROS), Cd content, and electrolyte leakage of the cells were enhanced by exogenous CdCl₂. When the Cd-induced accumulation of… More >

VIVIANE DAL-SOUTO FRESCURA¹, HAYWOOD DAIL LAUGHINGHOUSE IV², THAIS SCOTTI DO CANTODOROW¹, SOLANGE BOSIO TEDESCO^1*

BIOCELL, Vol.36, No.3, pp. 143-145, 2012, DOI:10.32604/biocell.2012.36.143

Abstract Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as ‘barba-de-São-João’, ‘barba-de-bode’, ‘vassourinha branca’, and ‘mimosa’. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander’s stain. The young inflorescences of twenty accessions were collected and fixed in a solution of ethanol: acetic acid (3:1) for 24 hours, then stored in ethanol 70% under refrigeration. Six slides per plant, two for each stain, were prepared by squashing, and 300 pollen grains per slide were analyzed. Pollen viability was high (>70%) for most accessions of… More >

Lazos-Monterrosa FA, C Orantes-García, O Farrera-Sarmiento, AG Verdugo-Valdez, MS Sánchez-Cortés, LE Ruíz-Meza

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 138-143, 2015, DOI:10.32604/phyton.2015.84.138

Abstract The tempisque (Sideroxylon capiri) is a tree native to Mexico used by the rural population for housing construction, poles and hedges, as fuel (wood) and also for fodder and ornamental purposes, among others. It is considered an endangered species. In order to contribute to its preservation and sustainable management, it was considered important to determine the proportion of viable seeds, the loss of viability due to storage period and the germination process by applying pregerminative treatments. We found that freshly collected seeds showed 100% viability, which decreased to 0% after 5 months of storage. According to the cumulative germination significant… More >

Rodríguez de León JI¹, MH Reyes-Valdés², DV Mendoza-Rodríguez³, F Ramírez-Godina², V Robledo-Torres², M Gómez-Martínez², G Hernández-Guzmán⁴

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 101-106, 2015, DOI:10.32604/phyton.2015.84.101

Abstract The cultivated husk tomato (Physalis ixocarpa) (2n = 2x = 24) is native from Mexico and Central America and shows a wide genetic variation. Presently, it is the fourth horticultural crop in cultivation surface in Mexico. The working team of this research previously developed an autotetraploid population by using colchicine. The objectives of the present work were to analyze the ploidy level and meiotic behavior of the subsequent generations (C3, C4, C5, C6) from the original (C2) composed only by plants with the duplicated genome from the Rendidora cultivar, and to determine pollen viability. As a diploid control the cultivar… More >

Alvarado-Vázquez MA, R Foroughbakhch, MA Guzmán- Lucio, A Rocha-Estrada, JL Hernández-Piñero, ML Cárdenas-Ávila, BM Soto-García

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 70-79, 2015, DOI:10.32604/phyton.2015.84.070

Abstract Candelilla (Euphorbia antisiphylitica Zucc.) is a very important plant resource in the arid lands of Northern Mexico. This is because the wax content coating the stem has unique properties which have been useful for multiple applications in the food industry, electronics, cosmetics, etc. However, the intensive exploitation of this resource has caused a great decrease in the populations of this species making necessary to consider strategies for their conservation and sustainable use. One of the primary needs with regeneration purposes is to know their reproductive processes, particularly the biotic and/or abiotic factors that determine the viability and germination of seeds.… More >

Astiz V¹, LF Hernández^2,3

Phyton-International Journal of Experimental Botany, Vol.83, pp. 27-36, 2014, DOI:10.32604/phyton.2014.83.027

Abstract The intra-plant pollen production in cultivated sunflower (Helianthus annuus L.) was quali- and quantitatively studied. Two modern self-pollinated, high oleic sunflower hybrids were grown during two consecutive seasons: 2009 in three planting dates, and 2010 in two planting dates. Two cross-pollinated varieties of sunflower, Hopi and Havasupai, were also studied to determine if an anomalous intracapitulum pollen gradient observed in preliminary studies could be a remnant character inherited from primitive genotypes. Pollen grains per flower (PGF), anther volume (AV), pollen grain volume (PGV) and pollen viability (PV) were measured in three capitulum sectors, external (ES), middle (MS) and internal (IS).… More >

Astiz V¹, LF Hernández^2,3

Phyton-International Journal of Experimental Botany, Vol.82, pp. 297-302, 2013, DOI:10.32604/phyton.2013.82.297

Abstract During microsporogenesis, sunflower florets might be exposed to episodes of day- and night-time temperatures exceeding 30 and 20 °C, respectively, that can affect pollen availability and consequently plant’s yield. The relationship between air temperature and relative humidity during the meiotic phase of microsporogenesis, and the quantity and quality of pollen produced by the flowers, were studied in two sunflower hybrids during two years. The hybrids were grown on irrigated plots in Bahía Blanca (38° 45’ S; 62° 11’ W) in three planting dates (PD) at a density of 5.6 plants/m2. Flowers were consecutively taken as anthesis progressed, sectorizing the capitulum… More >

Pereira JR¹, AE Duarte², JB Pitombeira³, MAP da Silva², NE de M Beltrão¹, LM Barros²

Phyton-International Journal of Experimental Botany, Vol.82, pp. 275-279, 2013, DOI:10.32604/phyton.2013.82.275

Abstract An experiment was conducted in dryland conditions of the Brazilian Northeast to determine the number of viable weed seeds (seedbank) in an upland cotton crop, and its distribution in the soil profile, before and after using various herbicide treatments. A randomized block design in a split-plot block scheme with 6 replications was used, where the main plots were constituted by a factorial (13 treatments and 2 sampling soil depths), and the subplots by 2 sampling dates. The seedbank was determined by germination of the recovered weed seeds obtained from different soil depths. The highest number of viable weed seeds in… More >

Flores-del Angel ML, R Foroughbakhch, A Rocha-Estrada, ML Cárdenas-Ávila, MA GuzmánLucio, YL Hernández-Aguilar, MA Alvarado-Vázquez

Phyton-International Journal of Experimental Botany, Vol.82, pp. 161-167, 2013, DOI:10.32604/phyton.2013.82.161

Abstract Candelilla (Euphorbia antisyphilitica Zucc.) is a native plant in the desert regions of northern Mexico and the southern United States. The primary commercial use of this plant is for wax production. This study assessed the morphology, viability, and the effects of temperature in combination with chemical and mechanical scarification on the germination of candelilla seeds. Seeds were collected in four localities in northeastern Mexico. The seeds were predominantly pyriform with foveolate surfaces and brown in color; on average, the seeds measured 2.92 ± 0.26, 1.91 ± 0.22, and 1.50 ± 0.41 mm in length, width, and thickness, respectively. The average… More >

Luján-Rangel¹ R, E Olivares-Sáenz², RE Vázquez-Alvarado², L Garza-Ocañas³, O Torres-Alanís³

Phyton-International Journal of Experimental Botany, Vol.76, pp. 61-77, 2007, DOI:10.32604/phyton.2007.76.061

Abstract Karwinskia parvifolia (Kp) and Karwinskia humboldtiana (Kh) are poisonous plants of the rhamnacea family. Toxicological and clinical studies performed with one of the compounds present in the fruit [peroxisomicine A1 (PA1)] suggest its possible pharmacological usefulness in the treatment of certain types of cancer. This is why these plants are cultivated: to increase their productivity of PA1. We studied the effects of nitrogen and species on seed viability. The germination curve was determined for Kp with (Kp-WN) and without (Kp-NN) nitrogen, and for Kh without nitrogen (Kh-NN) using the probit model. This is characterized by the probability of germination in… More >