JUNWEI DU, QIANG ZHANG, JING ZHANG, MAIERDANJIANG MAIHEMUTI, HAIYANG HE, RENBING JIANG^*

Oncology Research, Vol.33, No.2, pp. 357-367, 2025, DOI:10.32604/or.2024.050878 - 16 January 2025

Abstract Objectives: Melanoma is a highly aggressive and metastatic form of cancer, and the role of exosomal microRNAs (miRNAs) in its progression remains largely unexplored. This study aimed to investigate the effects of melanoma cell-derived exosomal miR-424-5p on angiogenesis and its underlying mechanisms. Methods: Exosomes were isolated from melanoma cell lines A375 and A2058, and their effects on the proliferation, migration, and angiogenesis of human umbilical vein endothelial cells (HUVECs) were examined. The interaction between miR-424-5p and its target gene, large tumor suppressor kinase 2 (LATS2), was analyzed using luciferase reporter assays and functional experiments. In vivo,… More >

ZHENGYANG ZHOU^1,#, LEI QIAO^1,#, TONGTONG WANG¹, WEN PAN¹, JINGJING DUAN¹, HAIYANG ZHANG², TING DENG¹, YI BA^1,*, YI HE^1,*

Oncology Research, Vol.33, No.2, pp. 327-345, 2025, DOI:10.32604/or.2024.050431 - 16 January 2025

Abstract Background: Patients with gastric cancer (GC) are prone to lymph node metastasis (LNM), which is an important factor for recurrence and poor prognosis of GC. Nowadays, more and more studies have confirmed that exosomes can participate in tumor lymphangiogenesis. An in-depth exploration of the pathological mechanism in the process of LNM in GC may provide effective targets and improve the diagnosis and treatment effect. Materials and Methods: We used sequencing analysis of collected serum to screen out exo-miRNA related to LNM in GC. ELISA, qRT-PCR, Western Blot, RNA pull-down assay, Transwell assay, animal experiments, and other… More > Graphic Abstract

TAO SUN^1,2, QINGHUA SONG³, HUA LIU^1,*

Oncology Research, Vol.33, No.1, pp. 133-148, 2025, DOI:10.32604/or.2024.054141 - 20 December 2024

Abstract Background: Lung cancer is a life-threatening disease that occurs worldwide, but is especially common in China. The crucial role of the tumour microenvironment (TME) in non-small cell lung cancer (NSCLC) has attracted recent attention. Cancer-associated fibroblasts (CAFs) are the main factors that contribute to the TME function, and CAF exosomes are closely linked to NSCLC. Methods: The expression levels of miR-3124-5p and Toll-interacting protein (TOLLIP) were analysed by bioinformatics prediction combined with RT-qPCR/Western Blot detection. Fibroblasts were isolated and identified from clinical NSCLC tissues. Transmission electron microscopy and Western Blot were used to identify exosomes… More >

Erdong Shen¹, Ting Pan¹, Pan Guo¹, Ke Chen¹, Rui Xu¹, Mei Yang¹, Dahe Zhan¹, Fang Fang¹, Qinghui Wu^1,*, Jianbing Hu^1,*

The International Conference on Computational & Experimental Engineering and Sciences, Vol.30, No.4, pp. 1-3, 2024, DOI:10.32604/icces.2024.012772

Abstract Objective
This study investigates the synergistic effects of a novel multifunctional nanoparticle on gastric cancer treatment through photothermal therapy, chemotherapy, and immunotherapy.

Method
Synthesize hollow mesoporous Prussian blue nanoparticles and load them with luteolin. Use exosomes to encapsulate the nanoparticles and modify the surface of the targeted peptide GX1. Detect the morphology of nanoparticles using a nanoparticle size analyzer and transmission electron microscopy. Use Coomassie Brilliant Blue to detect the effect of extracellular vesicle encapsulation. Detect the thermal conversion efficiency of nanoparticles under specific laser irradiation through infrared and ultraviolet spectroscopy, as well as the release rate… More >

LICHEN XU^1,2, PAN ZHANG^3,*, GUIQI ZHANG², ZHAOLIANG SHEN⁴, XIZHUANG BAI^1,3,*

Oncology Research, Vol.32, No.11, pp. 1777-1789, 2024, DOI:10.32604/or.2024.047704 - 16 October 2024

Abstract Background: Osteosarcoma (OS), recognized as the predominant malignant tumor originating from bones, necessitates an in-depth comprehension of its intrinsic mechanisms to pinpoint novel therapeutic targets and enhance treatment methodologies. The role of fat mass and obesity-associated (FTO) in OS, particularly its correlation with malignant traits, and the fundamental mechanism, remains to be elucidated. Materials and Methods: 1. The FTO expression and survival rate in tumors were analyzed. 2. FTO in OS cell lines was quantified utilizing western blot and PCR. 3. FTO was upregulated and downregulated separately in MG63. 4. The impact of FTO on the… More >

JIAJUN HUANG^1,#, WEI WANG^1,#, WENTONG LIN², HENGSEN CAI³, ZHIHAN ZHU¹, WAQAS AHMED⁴, QIANKUN ZHANG¹, JIALE LIU¹, YIFAN ZHANG¹, RONG LI¹, ZHINUO LI¹, AHSAN ALI KHAN⁵, DENG LU³, YONG HU⁶, LUKUI CHEN^1,*

BIOCELL, Vol.48, No.10, pp. 1405-1418, 2024, DOI:10.32604/biocell.2024.053148 - 02 October 2024

Abstract Degeneration and death of nerve cells are inevitable with the occurrence and progression of nervous system disorders. Researchers transplanted neural stem cells into relevant areas, trying to solve the difficulty of neural cell loss by differentiating neural stem cells into various nerve cells. In recent years, however, studies have shown that transplanted neural stem cells help neural tissues regenerate and return to normal through paracrine action rather than just replacing cells. Exosomes are essential paracrine mediators, which can participate in cell communication through substance transmission. In this regard, this review mainly discusses the current research More >

YI LI¹, TIANYI WANG¹, HAORAN DING¹, SHIYONG ZHUANG¹, XIAOBO DAI¹, BING YAN^1,2,*

Oncology Research, Vol.32, No.10, pp. 1623-1635, 2024, DOI:10.32604/or.2024.048191 - 18 September 2024

Abstract Background: Oral cancer, a malignancy that is prevalent worldwide, is often diagnosed at an advanced stage. MicroRNAs (miRNAs) in circulating exosomes have emerged as promising cancer biomarkers. The role of miRNA let-7c-5p in oral cancer remains underexplored, and its potential involvement in tumorigenesis warrants comprehensive investigation. Methods: Serum samples from 30 patients with oral cancer and 20 healthy controls were used to isolate exosomes and quantify their RNA content. Isolation of the exosomes was confirmed through transmission electron microscopy. Quantitative PCR was used to assess the miRNA profiles. The effects of let-7c-5p and TAGLN overexpression… More >

YUN WANG¹, XIAOJIANG LI², DALONG LIU², ZHIFENG WANG³, JICHEN XIA⁴, LIJUN WANG⁵, XUDONG ZHANG^6,*

Oncology Research, Vol.32, No.10, pp. 1649-1660, 2024, DOI:10.32604/or.2024.043482 - 18 September 2024

Abstract Exosomes, minute vesicles ubiquitously released by diverse cell types, serve as critical mediators in intercellular communication. Their pathophysiological relevance, especially in malignancies, has garnered significant attention. A meticulous exploration of the exosomal impact on cancer development has unveiled avenues for innovative and clinically valuable techniques. The cargo conveyed by exosomes exerts transformative effects on both local and distant microenvironments, thereby influencing a broad spectrum of biological responses in recipient cells. These membrane-bound extracellular vesicles (EVs) play a pivotal role in delivering bioactive molecules among cells and organs. Cellular and biological processes in recipient cells, ranging… More > Graphic Abstract

SHAN OU^#, YUN XU^#, QINGLAN LIU, TIANWEN YANG, WEI CHEN, XIU YUAN, XIN ZUO, PENG SHI^*, JIE YAO^*

BIOCELL, Vol.48, No.3, pp. 415-429, 2024, DOI:10.32604/biocell.2024.046178 - 15 March 2024

Abstract Background: The interrelation between intestinal polyps, metabolic syndrome (MetS), and colorectal cancer (CRC) is a critical area of study. This research focuses on pinpointing potential molecular targets to understand the link between intestinal polyp formation, metabolic irregularities, and CRC progression. Methods: We examined clinical samples from patients with intestinal polyps coexisting with MetS and compared them with samples from patients with standard intestinal polyps. Transcriptome sequencing and public database analysis were employed to identify significant pathways and genes. These targets were then validated through immunohistochemistry (IHC). Following the RNA interference of key target expression, a… More > Graphic Abstract

WEIXUE WANG^#, TONGTONG WANG^#, YAN ZHANG, TING DENG, HAIYANG ZHANG^*, YI BA^*

Oncology Research, Vol.32, No.3, pp. 489-502, 2024, DOI:10.32604/or.2023.046676 - 06 February 2024

Abstract Different from necrosis, apoptosis, autophagy and other forms of cell death, ferroptosis is a mechanism that catalyzes lipid peroxidation of polyunsaturated fatty acids under the action of iron divalent or lipoxygenase, leading to cell death. Apatinib is currently used in the third-line standard treatment of advanced gastric cancer, targeting the anti-angiogenesis pathway. However, Apatinib-mediated ferroptosis in vascular endothelial cells has not been reported yet. Tumor-secreted exosomes can be taken up into target cells to regulate tumor development, but the mechanism related to vascular endothelial cell ferroptosis has not yet been discovered. Here, we show that More >