Weifeng Ren^1,2,#, Xiaomeng Cai^2,3,#, Jun Chen^2,3,#, Lifo Ruan^2,3, Huiru Lu², Jiayu Zhang^2,3, Yi Hu^2,3,*, Jimin Gao^1,*

Oncologie, Vol.23, No.3, pp. 359-371, 2021, DOI:10.32604/Oncologie.2021.018605

Abstract This study reported the construction of GM-CSF-loaded chitosan/graphene oxide nanoparticles for photothermal therapy-assisted immunotherapy against murine orthotopic bladder cancer. The encapsulated GM-CSF in chitosan/graphene oxide nanoparticles facilitated their immobilization on biotinylated mouse orthotopic bladder cancer surface, resulting in slow release of antitumor immune adjuvant. Locally released GM-CSF efficiently recruited dendritic cells to bladder tumor sites, thereby promoting immune responses in mice. Thus, the activated immune response in mice provided enhanced antitumor effects. Meanwhile, the thermal effect from the nanoparticles upon light irradiation induced a significant increase in dendritic cells and T cells compared with other groups, which confirmed that the… More >

Peng Wang, Haiyang Zhang, Zilian Cui, Xunbo Jin^*, Dong Zhang^*

Oncologie, Vol.23, No.2, pp. 259-268, 2021, DOI:10.32604/Oncologie.2021.015503

Abstract Urothelial carcinoma of bladder (UCB) is a common urological malignancy in the world, but its progression mechanism remains unclear. MiR-612 was found as an anti-tumor factor in multiple types of cancer, while few studies have revealed its functions in UCB cells. Based on this, UCB cells such as HTB-9 and HTB-4, and normal urothelial of bladder cells such as SV-Huc1, were used as subjects in this study. Western blot, qRTPCR, CCK-8 assay and transwell assay were used to assess functions of miR-612 in UCB cells. The database, miRWalk, was used to search for potential targets of miR-612, and dual-luciferase reporter… More >

Ling Pan¹ , Yubo Tao^2,*, Peng Li^1,2,*

Journal of Renewable Materials, Vol.9, No.6, pp. 1099-1109, 2021, DOI:10.32604/jrm.2021.014683

Abstract As a kind of biopolymer, hydrolysates of fish swim bladder, safer than those of land mammals, are widely used in food, cosmetics as well as pharmaceutical and biomedical fields for their biocompatibility, biodegradability, and weak antigenicity. To enhance hydrolysate production, in this paper, the papain and alcalase hydrolysis processes of larimichthys polyactis swim bladder were optimized with orthogonal experiments. With 89.5% hydrolysate yield, the optimal processing conditions for alcalase were solid-liquid ratio of 1:30, enzyme concentration of 0.7%, and extraction time of 6 h. As for papain, under the optimal processing conditions: solid-liquid ratio of 1:20, enzyme concentration of 0.5%,… More >

Ahmed Talal, Osama Abu-Elnasr^*, Samir Elmougy

CMC-Computers, Materials & Continua, Vol.67, No.3, pp. 3487-3503, 2021, DOI:10.32604/cmc.2021.015759

Abstract Due to the demand of high computational speed for processing big data that requires complex data manipulations in a timely manner, the need for extending classical logic to construct new multi-valued optical models becomes a challenging and promising research area. This paper establishes a novel octal-valued logic design model with new optical gates construction based on the hypothesis of Light Color State Model to provide an efficient solution to the limitations of computational processing inherent in the electronics computing. We provide new mathematical definitions for both of the binary OR function and the PLUS operation in multi valued logic that… More >

QINGXIN LIU^1§ , XIANGGUI CHEN^2§, GUOLIN YANG¹ , XUEWEN MIN³ , AND MAOXIAN DENG^1,*

BIOCELL, Vol.35, No.3, pp. 71-80, 2011, DOI:10.32604/biocell.2011.35.071

Abstract Apigenin, a nonmutagenic flavonoid, has been shown to possess free radical scavenging activities, anticarcinogenic properties, antioxidant and anti-inflammatory effects. Recently, apigenin was reported to cause gastric relaxation in murine. To assess possible effects of apigenin on migration of bladder smooth muscle (SM) cell, we isolated SM cells from peri-cancer tissue of human bladder and established a cell model that was capable to overexpress transiently MEKK1 (MEK kinase 1). Results showed that overexpression of active human MEKK1 by adenoviruses infection induced migration of human bladder smooth muscle (hBSM) cells and phosphorylation of MAPKs, ERK, JNK and p38, which are the downstream… More >