QILU PAN^1,2,#, YAN JIANG^1,2,#, LINQIAO LI^1,2, XIAOJING DU¹, QIAN HAN¹, FEIXIANG LING¹, ROU LI¹, SHUYUAN CHU^1,2, LIN MAI¹, JIANWEI HUANG¹, LIBING MA^1,2,*

BIOCELL, Vol.47, No.11, pp. 2495-2502, 2023, DOI:10.32604/biocell.2023.042617

Abstract Introduction: Allergen-specific CD4⁺ T cells play a central role in autoimmune disorders, allergies and asthma, with Th2-type immunity being the typical functional response of CD4⁺ T cells. This study aimed to investigate the role of MBD2 in regulating Th2 cell differentiation. Methods: Splenic mononuclear cells were extracted from C57BL/6 mice, and CD4⁺ T cells were isolated using magnetic beads and confirmed through flow cytometry. Lentivirus was employed to construct MBD2-silenced CD4⁺ T cells. In vitro experiments were performed to treat splenogenic mononuclear cells and CD4⁺ T cells with Ovalbumin (OVA), and Th2 cell ratios and IL-4 levels were assessed using… More >

S. E. Burruel-Ibarra¹, R. A. Esquer-Osuna², R. G. Valdez-Melchor², D. A. Cuevas-Acuña², L. QuihuiCota³, J. Juárez⁴, J. C. Campos-García², E. Valbuena-Gregorio², M. A. López-Mata^2,*

Journal of Renewable Materials, Vol.11, No.6, pp. 2647-2663, 2023, DOI:10.32604/jrm.2023.027372

Abstract This study aimed to characterize the physical-chemical and biological properties of pectin (PC)/chia seed mucilage (CM) membranes. PC/CM [100/0 (control), 80/20%, 60/40%, and 40/60% w/w] membranes were prepared using the casting method. The membranes (PC/CM) were thin, yellow, lightly opaque (≈10%) and capable of blocking light UVB (between 66 at 52%). SEM analysis showed the presence of aggregates in the shape of a sphere (≈13 µm) and ovoid (≈25 µm). The proportion of 80/20 showed an increase in tensile strength (29%) and elastic modulus (19%) when compared to the control. FTIR analysis exhibited intermolecular interactions between PCPC, PC-CM, and CM-CM… More > Graphic Abstract

MINGHAO LI¹, YOULI QIU², WENHUI ZHANG¹, RUIHAO SUN¹, MEIJIN DU¹, LUZE YANG³, YU LI^1,*

BIOCELL, Vol.44, No.2, pp. 247-255, 2020, DOI:10.32604/biocell.2020.08835

Abstract While phthalate acid esters (PAEs) cannot fluoresce alone, they can be detected by fluorescence spectroscopy after chelation with bovine serum albumin (BSA). In this study, the types of amino acid residues at the active site of PAEs chelated with BSA were determined using molecular docking technology. A modification scheme of BSA with higher detection sensitivity fluorescence spectroscopy for PAEs was proposed based on the docking results and constructed for a novel BSA structure with a higher detection sensitivity of fluorescence spectroscopy using a homologous modeling method. Density functional theory (DFT) was employed to explore the influence before and after BSA… More >

Maximiliano GIRAUD-BILLOUD^{1, 2*}, Fernando EZQUER², Javiera BAHAMONDE², Marcelo EZQUER²

BIOCELL, Vol.41, No.1, pp. 1-12, 2017, DOI:10.32604/biocell.2017.41.001

Abstract A decrease in the tubular expression of albumin endocytic transporters megalin and cubilin has been associated with diabetic nephropathy, but there are no comprehensive studies to date relating early tubulointerstitial injury and the effect of the disease on both transporters in type 1 diabetes mellitus (T1DM). We used eight-weekold male C57BL/6 mice divided into two groups; one of them received the vehicle (control group), while the other received the vehicle + 200 mg/kg streptozotocin (T1DM). Ten weeks after the injection, we evaluated plasma insulin, enzymuria, urinary vitamin D-binding protein (VDBP), tubulointerstitial fibrosis and proximal tubule histology, markers of autophagy, and… More >