Cristiane Dos Santos VERGILIO, Edésio José Tenório De MELO^*

BIOCELL, Vol.37, No.2, pp. 45-54, 2013, DOI:10.32604/biocell.2013.37.045

Abstract Cadmium (Cd) induces several effects in different tissues, but our knowledge of the toxic effects on organelles is insufficient. To observe the progression of Cd effects on organelle structure and function, HuH-7 cells (human hepatic carcinoma cell line) were exposed to CdCl2 in increasing concentrations (1 μM – 20 μM) and exposure times (2 h – 24 h). During Cd treatment, the cells exhibited a progressive decrease in viability that was both time- and dose-dependent. Cd treated cells displayed progressive morphological changes that included cytoplasm retraction and nuclear condensation preceding a total loss of cell adhesion. Treatment with 10 μM… More >

Lidia M. Lopez*, Marta Dubin**, Patricia H. Carrizo**, Mario H. Burgos***, Amanda Pellegrino De Iraldi*, Andrés O. M. Stoppani**

BIOCELL, Vol.27, No.2, pp. 213-224, 2003, DOI:10.32604/biocell.2003.27.213

Abstract CG 10-248 (3,4-dihydro-2,2-dimethyl-9-chloro-2H-naphtho[1,2b]pyran- 5,6-dione; CG-NQ), a β-lapachone analogue, modified the ultrastructure of rat hepatocytes, as demonstrated by light and electron microscopy. After 4 h incubation with 100 µM CG-NQ, the following effects were observed: (a) nuclear chromatin condensation; (b) chromatin fragmentation; (c) displacement of mitochondria, concentrated around the nucleus; (d) disruption or expansion of mitochondrial outer or inner membranes, respectively; (e) displacement and alteration of endoplasmic reticulum (rough and smooth); (f) decrease of microvilli; (g) blebbing of plasma membrane and production of apoptotic bodies formed by folding of plasma membrane fragments around mitochondria or peroxysomes; and (h) production of hydrogen… More >

Runze Zhao¹, Tingting Xia¹, Mengyue Wang¹, Fan Feng¹, Wanqian Liu^1,*, Li Yang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 77-77, 2019, DOI:10.32604/mcb.2019.07155

Abstract This study was focus on the exploring the therapeutic function of lysyl oxidase (LOX) in rat nucleus pulposus (NP) cells in intervertebral disc degeneration (IVDD). To do this, a Sprague-Dawley (SD) rat caudal spine degeneration model was established by puncturing the Co5-6 disc. NP cells apoptosis and extracellular matrix (ECM) degeneration in IVDD were evaluated by real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR), Hematoxylin-Eosin (H&E) and immunofluorescence. Then, the therapeutic effect of LOX on IVDD was evaluated by histological staining. In vitro, the regulator effect of LOX on degenerate rat NP cell was explored. ECM relate proteins and cytokines were… More >

VIVIANE DAL-SOUTO FRESCURA¹, ANDRIELLE WOUTERS KUHN¹, HAYWOOD DAIL LAUGHINGHOUSE IV², JUÇARA TEREZINHA PARANHOS¹, SOLANGE BOSIO TEDESCO¹

BIOCELL, Vol.37, No.2, pp. 23-28, 2013, DOI:10.32604/biocell.2013.37.023

Abstract Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared… More >

LUCIA PRONSATO, ANABELA LA COLLA, ANA CAROLINA RONDA, LORENA MILANESI, RICARDO BOLAND, ANDREA VASCONSUELO^*

BIOCELL, Vol.37, No.1, pp. 1-9, 2013, DOI:10.32604/biocell.2013.37.001

Abstract Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (<20) or high (>60) passage numbers (identified as lC2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2 O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). When we analyzed the behavior of Bad, Bax, caspase-3 and mitochondrial transmembrane potential, we observed that cells in the h-C2C12 group were resistant to H2 O2 induction of apoptosis. We propose serially cultured C2C12… More >

Yanhong ZHEN¹, Li HAN², Kailai CAI¹, Lijun HUO¹, Hasan RIAZ¹, Canjie WU¹, Aixin LIANG¹ , Lei SANG¹, Liguo YANG^{1 *}

BIOCELL, Vol.38, No.1, pp. 17-24, 2014, DOI:10.32604/biocell.2014.38.017

Abstract Inhibins play important roles in the reproductive system. To evaluate whether inhibin α (1-32) fusion protein plays a role in cervical cancer growth, the plasmid pVAX-inhα was constructed and its effect on proliferation and apoptosis of the human cervical cancer cell line (Hela) was checked by flow cytometry and real-time PCR. The expression and localization of inhibin α protein were detected by RT-PCR and confocal microscopy which showed that inhibin α protein was expressed and localized in the nucleus of Hela cells. Over expression of inhibin α gene significantly induced cell apoptosis and ceased S phase of cell cycle. Furthermore,… More >

Hong YE^1,6,#, WeiWei Wang^2,#, Yu Ding^3,#, XiaoLei Liu^4,#, WenJI Jia¹, WeiLi Luo¹, HuiJuan Fan¹, HongQun Zhou¹, Jin Wang¹, JianLong Ju¹, DongMing Zhou⁷, TianHao Bao^5,1,*, YuHong Zhu^1,*

BIOCELL, Vol.42, No.3, pp. 79-86, 2018, DOI:10.32604/biocell.2018.04615

Abstract Ischemic postconditioning (IP) has been shown to provide protection for ischemia/reperfusion (IR) injury, but its efficacy is limited. In this study we hypothesized that spontaneous running wheel (RW) could improve neuroprotection efficacy of IP for IR. We established mouse models of IR and showed that compared to Sham group, IR group had obvious brain infract and neurological dysfunction. In IR+IP group, brain infract and neurological dysfunction improved compared to IR group. However, in IR+IP+RW group brain infract and neurological dysfunction improved much better. TUNEL assay showed that IP but not RW significantly reduced the number of apoptotic cells after IR.… More >

Estela M. MUÑOZ

BIOCELL, Vol.42, No.2, pp. 41-46, 2018, DOI:10.32604/biocell.2018.07011

Abstract Until recently, microglia were mainly known as the resident phagocytes of the brain, i.e. the ‘immunological warriors’ of the brain. However, extensive knowledge is being accumulated about the functions of microglia beyond immunity. Nowadays, it is well accepted that microglial cells are highly dynamic and responsive, and that they intervene in a dual manner in many developmental processes that shape the central nervous system, including neurogenesis, gliogenesis, spatial patterning, synaptic formation and elimination, and neural circuit establishment and maturation. The differentiation and the pool of precursor cells were also shown to be under microglia regulation via bidirectional communication. In this… More >

Atif Kamil¹, Mubarak Ali Khan¹, Muhammad AAsim², Nadir Zaman Khan², Raham Sher Khan¹, Muhsin Jamal³, Waqar Ahmad⁴, Mir Azam Khan⁴, Fazal Jalil⁴

BIOCELL, Vol.43, No.3, pp. 167-174, 2019, DOI:10.32604/biocell.2019.06729

Abstract Several toxic compounds are known to induce apoptosis in mammalian cell lines. The human neuroblastoma cells (SH-SY5Y) were exposed to the phosphatase inhibiting toxin okadaic acid (OA) or hydrogen peroxide (H2O2) to induce apoptosis as well as generate reactive oxygen species (ROS). Mitoxantrone (MXT) was used as a positive control for apoptosis. The SH-SY5Y cells were transfected with eukaryotic expression plasmid pHyPer-dMito encoding mitochondrial-targeted fluorescent or pHyPer-dCito encoding cytoplasmic-targeted fluorescent sensor for hydrogen peroxide (HyPer). The ERp57, also called GRP58 (Glucose-regulated protein 58), is a stress protein induced in conditions like glucose starvation and viral infection. Recently ERp57 was shown… More >

Ji SHI; Peixin SUN, Ye ZHANG, Bing YAO, Haozhe PIAO

BIOCELL, Vol.43, No.3, pp. 119-128, 2019, DOI:10.32604/biocell.2019.07577

Abstract Glioma is the most common primary malignant brain tumor with a poor survival rate. In recent years, no significant progress has been made in the treatment of gliomas in contrast to the development of improved diagnosis via molecular typing. Newcastle disease virus (NDV), a negative-stranded RNA virus that exhibits oncolytic activity, has been investigated for its capacity to elicit antitumor activity in many types of cancers, including glioma. Therefore, application of oncolytic viruses, such as NDV, as a new treatment strategy to specifically target aberrant signaling in glioblastomas has brought new hope. For many years, NDV has been investigated for… More >