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  • Open Access

    ARTICLE

    Influence of estradiol analogue on cell growth, morphology and death in esophageal carcinoma cells

    THANDI MQOCO1, SUMARI MARAIS1 AND ANNIE JOUBERT

    BIOCELL, Vol.34, No.3, pp. 113-120, 2010, DOI:10.32604/biocell.2010.34.113

    Abstract 2-Methoxyestradiol-bis-sulphamate is a bis-sulphamoylated derivative of the naturally occurring 17-beta-estradiol metabolite namely 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate is regarded as a potential anticancer drug with increased antiproliferative activity when compared to 2-methoxyestradiol. The aim of this pilot in vitro study was to determine the influence of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and possible induction of certain types of cell death in the SNO esophageal carcinoma cell line. A dose-dependent study (0.2-1.0μM) was conducted with an exposure time of 24 hours. Data revealed that 2-methoxyestradiol-bis-sulphamate reduced cell numbers statistically significantly to 74% after exposure to 0.4μM of the drug. Morphological studies including light microscopy… More >

  • Open Access

    ARTICLE

    In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line

    CHRIS VORSTER, ANNIE JOUBERT

    BIOCELL, Vol.34, No.2, pp. 71-80, 2010, DOI:10.32604/biocell.2010.34.071

    Abstract In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM’s in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4μM. In addition, mitotic indices revealed that 2ME-BM induces a G2M block. The latter was confirmed by flow cytometric analyses where… More >

  • Open Access

    REVIEW

    Minireview: C2- and C4-position 17β-estradiol metabolites and their relation to breast cancer

    ANNIE JOUBERT1*, HERMIA VAN ZYL1, JOHANNES LAURENS2, MONA-LIZA LOTTERING1

    BIOCELL, Vol.33, No.3, pp. 137-140, 2009, DOI:10.32604/biocell.2009.33.137

    Abstract C2- and C4-position 17β-estradiol metabolites play an important role in breast carcinogenesis. 2-Hydroxyestradiol and 4-hydroxyestradiol are implicated in tumorigenesis via two pathways. These pathways entail increased cell proliferation and the formation of reactive oxygen species that trigger an increase in the likelihood of deoxyribonucleic acid mutations.
    2-Methoxyestradiol, a 17β-estradiol metabolite, however, causes induction of apoptosis in transformed and tumor cells; thus exhibiting an antiproliferative effect on tumor growth. The 4-hydroxyestradiol:2- methoxyestradiol and 2-hydroxyestradiol:2-methoxyestradiolratios therefore ought to be taken into account as possible indicators of carcinogenesis. More >

  • Open Access

    ARTICLE

    DNA mismatch repair gene methylation in gastric cancer in individuals from northern Brazil

    ELEONIDAS MOURA LIMA1,2, MARIANA FERREIRA LEAL3, MARÍLIA DE ARRUDA CARDOSO SMITH3, ROMMEL RODRÍGUEZ BURBANO4, PAULO PIMENTEL DE ASSUMPÇÃO5, MARIA JOSE BELLO6, JUAN ANTONIO REY6, FRANCINALDO FERREIRA DE LIMA7, CACILDA CASARTELLI2

    BIOCELL, Vol.32, No.3, pp. 237-243, 2008, DOI:10.32604/biocell.2008.32.237

    Abstract Gastric cancer is one of the most common malignancies. DNA methylation is implicated in DNA mismatch repair genes deficiency. In the present study, we evaluated the methylation status of MLH1, MSH2, MSH6 and PMS2 in 20 diffuse- and 26 intestinal-type gastric cancer samples and 20 normal gastric mucosal of gastric cancer patients from Northern Brazil. We found that none of the nonneoplastic samples showed methylation of any gene promoter and 50% of gastric cancer samples showed at least one methylated gene promoter. Methylation frequencies of MLH1, MSH2, MSH6 and PMS2 promoter were 21.74%, 17.39%, 0% and 28.26% respectively in gastric… More >

  • Open Access

    ARTICLE

    Decrease of intestinal tumors induced by 1,2-dimethylhydrazine in rats fed with cow milk and buffalo milk

    M. Sánchez Negrette, M.A. Montenegro, M.S. Catuogno, W.J. Lértora, M.C. Guanziroli

    BIOCELL, Vol.31, No.3, pp. 391-396, 2007, DOI:10.32604/biocell.2007.31.391

    Abstract Epidemiological studies in human beings and experimental studies in laboratory animals suggest that milk and dairy products can inhibit effects on the development of some kinds of tumors. Cow milk contains sphingomyelin, butyric acid, conjugated linoleic acid, calcium, vitamin A, carotene and vitamin D. All of these components are known to inhibit the process of carcinogenesis. Our objective was to determine the effect of cow milk and water buffalo milk on the development of colon neoplasias in an experimental model of carcinogenesis in rats induced with 1,2-dimethylhydrazine (DMH). Three-month-old Wistar male rats with an average body weight of 180 g… More >

  • Open Access

    ARTICLE

    Inhibition of focal adhesion kinase by antisense oligonucleotides enhances the sensitivity of breast cancer cells to camptothecins

    T.H. Satoh2, T.A. Surmacz3, O. Nyormoi4, C.M. Whitacre1

    BIOCELL, Vol.27, No.1, pp. 47-55, 2003, DOI:10.32604/biocell.2003.27.047

    Abstract This study shows a strong association between cell attachment to substratum and activation of β1-integrin-signaling with resistance to the camptothecin derivative topotecan (TPT) in breast cancer cells. We propose a mechanistic-driven approach to sensitize the cells to camptothecins. ZR-75-1 anchoragedependent breast cancer cell line, its derivative 9D3S suspension cells (9D3S-S), and 9D3S cells attached to fibronectin-coated plates (9D3S-A) were treated with TPT (1 µM) or CPT-11 (40 µM) for 48 h. Programmed cell death (PCD), as shown by poly(ADP-ribose) polymerase (PARP), pro-caspase-3 and pro-caspase-9 cleavage, was observed in 9D3S-S cells but not in ZR-75-1 or 9D3S-A cells. Because p125 focal… More >

  • Open Access

    ABSTRACT

    Effects of Three-Dimensional Stiffness on the Proliferation, Stemness And Invasion of Hepatic Cancer Stem Cells

    Mengyue Wang1, Runze Zhao1, Fan Feng1, Tingting Xia1,*, Li Yang1,*

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 130-130, 2019, DOI:10.32604/mcb.2019.07154

    Abstract Hepatocellular carcinoma (HCC) is the third most common cancer in the world. Previous studies have shown that hard matrix promotes the proliferation of liver tumor cells. However, the role of matrix stiffness on hepatic cancer stem cells (HCSCs) is still unclear. Three-dimensional hydrogels with different stiffness were used to mimic the normal liver tissue (4kPa) and cancerous liver tissue (26kPa) stiffness. The proliferation, stemness and invasion properties of HCSCs under 3D different stiffness were detected. METHOD: HSCSs were screened and cultured by enrichment method, and the effect of matrix stiffness on HCSCs was studied by three-dimensional culture of HCSCs in… More >

  • Open Access

    ABSTRACT

    In Vivo Biomechanical Measurements of Benign and Cancerous Skin Lesions Using Vibrational Oct

    Frederick H. Silver1,*, Ruchit G. Shah2, Dominick Benedetto3

    Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 73-73, 2019, DOI:10.32604/mcb.2019.08150

    Abstract Previous literature reports suggest that tissue stiffness is a predictor of cancer and metastatic behavior of lesions. We have used optical coherence tomography and vibrational analysis (VOCT) to characterize normal skin, scar, a verrucous carcinoma (a squamous cell carcinoma subtype), a basal cell carcinoma and benign skin lesions non-invasively and non-destructively. The results suggest that epidermal thickening and increased keratin and basal cell production occur in malignant lesions and lead to increases in surface hills and valleys as well as subsequent increases in epidermal stiffness values. Increased stiffness of the epidermis is a result of increased keratin and basal cell… More >

  • Open Access

    ARTICLE

    Overexpression of inhibin α (1-32) fusion protein promotes apoptosis and cell cycle arrest in a cervical cancer cell model (Hela cells)

    Yanhong ZHEN1, Li HAN2, Kailai CAI1, Lijun HUO1, Hasan RIAZ1, Canjie WU1, Aixin LIANG1 , Lei SANG1, Liguo YANG1 *

    BIOCELL, Vol.38, No.1, pp. 17-24, 2014, DOI:10.32604/biocell.2014.38.017

    Abstract Inhibins play important roles in the reproductive system. To evaluate whether inhibin α (1-32) fusion protein plays a role in cervical cancer growth, the plasmid pVAX-inhα was constructed and its effect on proliferation and apoptosis of the human cervical cancer cell line (Hela) was checked by flow cytometry and real-time PCR. The expression and localization of inhibin α protein were detected by RT-PCR and confocal microscopy which showed that inhibin α protein was expressed and localized in the nucleus of Hela cells. Over expression of inhibin α gene significantly induced cell apoptosis and ceased S phase of cell cycle. Furthermore,… More >

  • Open Access

    ARTICLE

    Effects of high molybdenum intake on 1,2-dimethylhydrazine-induced intestinal tumors in rats

    M.A. MONTENEGRO*, M. SÁNCHEZ NEGRETTE*, E.J. GIMENO**, J.T. BORDA*

    BIOCELL, Vol.26, No.3, pp. 339-345, 2002, DOI:10.32604/biocell.2002.26.339

    Abstract Wistar male rats, 3 months of age were given ad-libitum a nutritionally adequate diet and demineralized drinking water. The Molybdenum (Mo) and Tungsten (W) were provided in the drinking water at 200 ppm concentration. Intestinal tumors were induced by 1,2-dimethylhydrazine (DMH) given subcutaneously as 16 weekly doses at 20 mg/kg body weight. Mo in the form of (NH4)6 Mo7O24 4H2O or W in the form of (Na2 WO4) were provided in the drinking water two months before the first DMH treatment and were continued during 4 months more until the last DMH treatment. Three months after the last carcinogen injection,… More >

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