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  • Open Access

    ARTICLE

    Functional Studies of Castor (Ricinus communis L.) PLC Family Genes in Arabidopsis Inflorescence Development

    Yanpeng Wen1,#, Rui Luo1,#, Lili Li1, Xiaotian Liang1, Xuemei Hu1, Ruxin Li1, Zhiyan Wang1, Fenglan Huang1,2,3,4,5,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3091-3112, 2023, DOI:10.32604/phyton.2023.030960

    Abstract Castor (Ricinus communis L.) is one of the top 10 oil crops in the world, and inflorescence is a trait that directly affects its yield. Phospholipase C (PLCs) is involved in many plant activities and metabolic processes. To study the functions of PLC family genes in the regulation of the inflorescence development of the female line of Lm-type castor aLmAB2, we determined the expression levels of six PLC family genes of three types of inflorescences of aLmAB2 (isofemale line, female line, bisexual line) at different developmental stages. The results showed that the 6 genes of the castor PLC family had… More >

  • Open Access

    ARTICLE

    Genome-Wide Analysis of the F3′5′H Gene Family in Blueberry (Vaccinium corymbosum L.) Provides Insights into the Regulation of Anthocyanin Biosynthesis

    Xiaolan Guo1,2, Jinbin Hu3, Shimei Yang4, Delu Wang2,*, Jianbing Wang1

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2683-2697, 2023, DOI:10.32604/phyton.2023.029820

    Abstract The F3′5′H gene family plays an important role in regulating anthocyanin biosynthesis, abiotic stress, and hormone signaling. In this study, 14 F3′5′H genes were identified from the blueberry genome. The chromosomal distribution, physicochemical properties, F3′5′H domain, conserved motifs, cis-acting elements, and intron/exon compositions were analyzed. The functional prediction analysis of these VcF3′5′Hs indicated that their biological functions included light response and other secondary metabolites. The results of qRT-PCR showed that VcF3′5′Hs (especially VcF3′5′H4) were highly expressed at the ripening stage. Subcellular localization revealed that VcF3′5′H4 may be located in the endoplasmic reticulum. Co-expression analysis showed that the VcF3′5′H gene family… More >

  • Open Access

    ARTICLE

    Identification of the SERK Gene Family in Paulownia fortunei and Its Involvement in the Response to Biotic and Abiotic Stresses

    Tongman Zhao1,2,#, Yujie Fan1,2,#, Yabing Cao1,2, Xiaogai Zhao1,2, Guoqiang Fan1,2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2473-2488, 2023, DOI:10.32604/phyton.2023.029469

    Abstract Somatic embryogenesis receptor-like kinases (SERKs) are receptor-like proteins that contain leucine-rich repeats and are involved in various signaling pathways. This study identified SERK family members in the Paulownia fortunei genome and analyzed their characteristics and expression profiles using bioinformatics methods. We identified 12 SERK genes with relatively conserved gene structures and motifs that were distributed unevenly on eight Paulownia chromosomes. The gene promoters contained various cis-acting elements that regulated the expression of the PfSERK genes in response to hormones and abiotic stresses. Synteny analysis indicated that 10 segmental duplication events had occurred during evolution of the PfSERK family. The expression… More >

  • Open Access

    ARTICLE

    Exploring the Experiences of Personal Recovery among Mental Health Consumers and Their Caregivers Receiving Strength-Based Family Interventions

    Li-yu Song1,*, Su-ting Hsu2

    International Journal of Mental Health Promotion, Vol.25, No.8, pp. 915-925, 2023, DOI:10.32604/ijmhp.2023.019349

    Abstract Background: This study explored the personal recovery of consumers and their caregivers receiving the strength-based family intervention. Method: A three-year project was implemented with 43 dyads from 5 community psychiatric rehabilitation agencies in northern, central, and Southern Taiwan. This paper presents qualitative analysis with a focus on describing the experiences of personal recovery. To gain a deeper understanding of the participants’ personal experiences and perspectives, semi-structured in-depth interviews were conducted on three occasions (six months after the inception of the experiment, 18 months after, and when the participants left the services of this study). Over the three occasions, a total… More >

  • Open Access

    ARTICLE

    Biological Analysis and Response to Low Phosphate Stress of Phosphate Transporter Family 1 (PHT1)) Genes in Solanum tuberosum L.

    Yangyang Du1, Yuanyuan Bao2, Lu Zheng1, Qinglong Tian1, Xinyong Zhang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2325-2348, 2023, DOI:10.32604/phyton.2023.027642

    Abstract Inorganic phosphate (Pi) is one of the main nutrients necessary for plant growth. Phosphate transporters mediate the acquisition, transport and recycling of phosphate, which is essential for plant growth and development. Although PHT1 has been reported in many plants at home and abroad, it is rarely studied in potato. Therefore, it is of great significance to study the PHT1 family members in order to understand the molecular response mechanism of potato in low phosphate state. In this study, a total of 6 potato PHT1 genes were identified and isolated. It was found that after the expression of different members of… More >

  • Open Access

    ARTICLE

    A Novel Multi-Stage Bispectral Deep Learning Method for Protein Family Classification

    Amjed Al Fahoum*, Ala’a Zyout, Hiam Alquran, Isam Abu-Qasmieh

    CMC-Computers, Materials & Continua, Vol.76, No.1, pp. 1173-1193, 2023, DOI:10.32604/cmc.2023.038304

    Abstract Complex proteins are needed for many biological activities. Folding amino acid chains reveals their properties and functions. They support healthy tissue structure, physiology, and homeostasis. Precision medicine and treatments require quantitative protein identification and function. Despite technical advances and protein sequence data exploration, bioinformatics’ “basic structure” problem—the automatic deduction of a protein’s properties from its amino acid sequence—remains unsolved. Protein function inference from amino acid sequences is the main biological data challenge. This study analyzes whether raw sequencing can characterize biological facts. A massive corpus of protein sequences and the Globin-like superfamily’s related protein families generate a solid vector representation.… More >

  • Open Access

    ARTICLE

    Genome-Wide Analysis of the KANADI Gene Family and Its Expression Patterns under Different Nitrogen Concentrations Treatments in Populus trichocarpa

    Minghui Niu1,#, Heng Zhang1,#, Xiangyang Li1, Zhibao Hu1, Hongjiao Zhang2,3, Zhiru Xu2,3, Chunpu Qu1,4,*, Guanjun Liu1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2001-2015, 2023, DOI:10.32604/phyton.2023.029350

    Abstract KANADI (KAN) is a plant-specific gene that controlled the polarity development of lateral organs. It mainly acted on the abaxial characteristics of plants to make the lateral organs asymmetrical. However, it had been less identified in woody plants. In this study, the members of the KAN gene family in Populus trichocarpa were identified and analyzed using the bioinformatics method. The results showed that a total of 8 KAN family members were screened out, and each member contained the unique GARP domain and conserved region of the family proteins. Phylogenetic analysis and their gene structures revealed that all KAN genes from… More >

  • Open Access

    ARTICLE

    The extracellular secretion of miR-1825 wrapped by exosomes increases CLEC5A expression: A potential oncogenic mechanism in ovarian cancer

    QIAOLING WU1,2, ZHAOLEI CUI3, HONGMEI XIA1, SHAN JIANG1, JING BAI4, ZHUO SHAO4, YANG SUN1,*

    BIOCELL, Vol.47, No.5, pp. 1039-1050, 2023, DOI:10.32604/biocell.2023.027494

    Abstract Background: Ovarian cancer (OC) is a leading cause of gynecological cancer-linked deaths worldwide. Exosomal miR-1825 and its target gene C-type lectin domain family 5 member A (CLEC5A) are associated with tumorigenesis in cancers that was further probed. Methods: Exosomal miR-1825 expression in exosomes and its impact on overall survival (OS) prediction were determined using Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) data. Target genes of miR-1825 were searched in five prediction databases and prognostically significant differentially expressed genes were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were carried out. The… More >

  • Open Access

    ARTICLE

    An Innovative Bispectral Deep Learning Method for Protein Family Classification

    Isam Abu-Qasmieh, Amjed Al Fahoum*, Hiam Alquran, Ala’a Zyout

    CMC-Computers, Materials & Continua, Vol.75, No.2, pp. 3971-3991, 2023, DOI:10.32604/cmc.2023.037431

    Abstract Proteins are essential for many biological functions. For example, folding amino acid chains reveals their functionalities by maintaining tissue structure, physiology, and homeostasis. Note that quantifiable protein characteristics are vital for improving therapies and precision medicine. The automatic inference of a protein’s properties from its amino acid sequence is called “basic structure”. Nevertheless, it remains a critical unsolved challenge in bioinformatics, although with recent technological advances and the investigation of protein sequence data. Inferring protein function from amino acid sequences is crucial in biology. This study considers using raw sequencing to explain biological facts using a large corpus of protein… More >

  • Open Access

    ARTICLE

    Genome-Wide Identification and Expression Analysis of the Phytocyanin Gene Family in Nicotiana tabacum

    Peiling Wang1,#, Xiaohong Xu2,#, Yong Li2, Hecui Zhang1, Xuejie Zhang3, Siru Zhou1, Yimei Liu1, Yunyan Feng1, Tonghong Zuo1, Liquan Zhu1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1469-1492, 2023, DOI:10.32604/phyton.2023.027133

    Abstract Phytocyanin (PC) is a class of plant-specific blue copper proteins involved in electron transport, plant growth, development, and stress resistance. However, PC proteins have not been systematically evaluated in tobacco plants. We determined the whole-genome sequences of the PC family in the tobacco cultivar ‘K326.’ The transcriptome data were used to analyze the expression of the NtPC family at different development stages and tissue-specific genes. Real-time fluorescence quantitative analysis was used to analyze the expression of the NtPC gene family under low temperature and methyl jasmonate stress. The tobacco NtPC family contained 110 members and was divided into four subfamilies:… More >

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