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  • Open Access

    ARTICLE

    Preparation of Tartary Buckwheat Seed Coating Agent and Its Effect on Germination

    Xin Zou1, Jieyu Zhang1, Ting Cheng1, Yangyang Guo1, Xiao Han1, Han Liu1, Yuxing Qin1, Jie Li2, Dabing Xiang1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.4, pp. 699-712, 2024, DOI:10.32604/phyton.2024.048469

    Abstract To mitigate the wastage of seed resources and reduce the usage of pesticides and fertilizers, seed coating agents have gained popularity. This study employs single-factor and multi-index orthogonal experimental design methods to investigate the seed coating formula and physical properties of Tartary buckwheat. The specific effects of each component on Tartary buckwheat seed germination are analyzed. The findings reveal that the seed coating agent formulated with 1.5% polyvinyl alcohol, 0.15% sodium alginate, 0.2% op-10, 0.1% polyacrylamide, 8% colorant, 3% ammonium sulfate, 1% potassium dihydrogen phosphate, and 0.15% carbendazim exhibits the most effective coating. It demonstrates… More >

  • Open Access

    ARTICLE

    The Combination of Achnatherum inebrians Extracts and Soil Microorganisms Inhibited Seed Germination and Seedling Growth in Elymus nutans

    Rui Zhang1, Taixiang Chen1, Zhenjiang Chen1, Hao Chen1, Xuekai Wei1, Malik Kamran1, Chunjie Li1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 567-580, 2024, DOI:10.32604/phyton.2024.047485

    Abstract In a greenhouse experiment, the effects of soil microorganisms and extracts of Achnatherum inebrians on the seed germination and seedling growth of Elymus nutans were studied. The results showed that both the extracts from aboveground and belowground parts of A. inebrians significantly inhibited the germination rate, germination potential, germination index, vigor index, seedling height, root length, and fresh weight of E. nutans, but increased malondialdehyde content, catalase, peroxidase and superoxide dismutase activity of E. nutans seedlings (p < 0.05). The allelopathy of aqueous extracts of the aboveground parts of A. inebrians was stronger than that of the precipitates. Aqueous extracts of the… More >

  • Open Access

    ARTICLE

    Comparative Transcriptome Analysis of Seed Germination of a Cotton Variety with High Tolerance to Low Temperature

    Genhai Hu1,*, Maoni Chao1, Xiuren Zhou2, Yuanzhi Fu2

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2535-2554, 2023, DOI:10.32604/phyton.2023.030163

    Abstract Gossypium hirsutum L. is an important cash crop native to the subtropics and is widely cultivated around the world. Low temperature is an important stress that seriously affects seed germination and emergence during planting. In this study, transcriptomic profiles of low-temperature- and normal-temperature-germinated seeds of Xinluzao 25, a variety with low-temperature tolerance and high germination rates, were analyzed and compared. The following results were obtained. (1) A total of 81.06 Gb of clean data were obtained after transcriptome sequencing and assembly, and 76,931 non-redundant Unigene sequences were obtained after data consolidation and concatenation; of these, 69,883… More >

  • Open Access

    ARTICLE

    Substrate, Hormone, Winnowing, and Stratification Influence the Seed Germination of Ilex asprella (Hook. et Arn.) Champ. ex Benth

    Yuquan Huang1,2, Fan Wei1, Qing Ma2, Yang Lin1, Jinpeng Huang2, Yanxia Zhu1,*, Danfeng Tang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2105-2116, 2023, DOI:10.32604/phyton.2023.029205

    Abstract Ilex asprella (Hook. et Arn.) Champ. ex Benth is one of the most important traditional Chinese medicines in southern China. The seeds of Ilex asprella usually have extremely low germination due to their dormancy characteristics, which severely impacts the efficiency of seedling raising and increases labor costs. In this study, to improve the seed germination of I. asprella, the effects of germination substrate, hormone, winnowing, and stratification treatments on the seed germination of I. asprella were investigated. The results of the germination matrix showed that the highest germination percentage of 45.2% was achieved under the 20°C/10°C day/night temperature and… More >

  • Open Access

    ARTICLE

    Identification and Expression Analysis of Abscisic Acid Signal Transduction Genes in Hemp Seeds

    Cong Hou1, Kang Ning1, Xiuye Wei1, Yufei Cheng1, Huatao Yu1, Haibin Yu2, Xia Liu1,*, Linlin Dong1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2087-2103, 2023, DOI:10.32604/phyton.2023.029041

    Abstract Abscisic acid (ABA) is involved in regulating diverse biological processes, but its signal transduction genes and roles in hemp seed germination are not well known. Here, the ABA signaling pathway members, PYL, PP2C and SnRK2 gene families, were identified from the hemp reference genome, including 7 CsPYL (pyrab-actin resistance1-like, ABA receptor), 8 CsPP2CA (group A protein phosphatase 2c), and 7 CsSnRK2 (sucrose nonfermenting1-related protein kinase 2). The content of ABA in hemp seeds in germination stage is lower than that in non-germination stage. Exogenous ABA (1 or 10 μM) treatment had a significant regulatory effect on the selected PYL,… More >

  • Open Access

    ARTICLE

    Observation of Flowering Process of Grape (Vitis vinifera L.)—Insight into the Starting of Pollination of Flower Hiding in Calyptras (Cap)

    Yue Wang1,#, Xiujie Li2,#, Yusen Wu2, Zhonghui Cai1, Bo Li2, Zhaosen Xie1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1859-1871, 2023, DOI:10.32604/phyton.2023.027067

    Abstract It is generally agreed that many Vitis vinifera L. cultivars are self-fertile, where self-pollination often occurs before capfall in a process called cleistogamy. Therefore, it is difficult to identify the right time to remove stamens before self-pollination during the cross-breeding of grape. For this paper, we observed the process of grape flowering and measured the pollen viability and stigma receptivity of grape flowers of ‘Shine Muscat’ in order to identify the starting time of self-pollination before capfall and to provide useful information for improving the efficiency of cross-breeding. The results demonstrate that the anther is not… More >

  • Open Access

    ARTICLE

    Impact of ZnSO and ZnO Nanoparticles on Seed Germination and Seedling Growth of Lettuce

    Adalberto Benavides-Mendoza1, Rebeca Betancourt-Galindo2, Nazario Francisco-Francisco3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1831-1840, 2023, DOI:10.32604/phyton.2023.028085

    Abstract

    Micronutrient nanoparticles (NPs) are currently an option for chemical fertilization and biostimulation in crops. However, there is little information on the phytotoxic or biostimulatory effects of NPs at low concentrations of some elements, such as Zn. In this study, the effect of low concentrations of Zn oxide (ZnO) NPs on germination, growth variables, and nutritional attributes of lettuce (Lactuca sativa L.) was evaluated in comparison to Zn sulfate. Romaine lettuce seeds were treated with ZnSO4-- × 7H2O and ZnO NPs at Zn molar concentrations of 1 × 10−3, 5 × 10−3, 1 × 10−4, 5 × 10−4, 1

    More >

  • Open Access

    ARTICLE

    Integrated Transcriptome and Small RNA Sequencing Analyses Reveals Insights into the Molecular Mechanism of Seed Germination in Mung Bean

    Yanyan Pu1,#, Liwen Wang2,#, Leilei Li3, Yujun Si4, Shubin Xie2, Yunzhe Cong1, Dong Wang1, Yongchao Gong1, Rumei Tian1, Xue Chen3, Xiaoyan Zhang1, Min Liu1, Hanfeng Ding1,3,*, Nana Li1,3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1793-1812, 2023, DOI:10.32604/phyton.2023.026822

    Abstract During the life cycle of a plant, seed germination is crucial. Upon ingestion of water, the dry seeds resumed energy metabolism and cellular repair. To dissect the complex mechanisms at the very beginning of seed germination, two approaches including transcriptome and small RNA sequencing were conducted during the water imbibition process of mung bean seeds compared with dry seed. The transcriptome sequencing analysis identified 10,108 differentially expressed genes (DEGs) between dry and imbibed mung bean seeds. KEGG enrichment analyses demonstrated numerous DEGs involved in hormone signaling pathways, carbohydrate, and energy metabolism. Out of the total… More >

  • Open Access

    ARTICLE

    Melatonin Promotes Rice Seed Germination under Drought Stress by Regulating Antioxidant Capacity

    Luqian Zhang1,#, Xilin Fang1,#, Nan Yu1, Jun Chen1, Haodong Wang1, Quansheng Shen1, Guanghui Chen2,*, Yue Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1571-1587, 2023, DOI:10.32604/phyton.2023.025481

    Abstract Drought stress is a serious threat to the germination of plant seeds and the growth of seedlings. Melatonin has been proven to play an important role in alleviating plant stress. However, its effect on seed germination under drought conditions is still poorly understood. Therefore, we studied the effects of melatonin on rice seed germination and physiological characteristics under drought stress. Rice seeds were treated with different concentrations of melatonin (i.e., 0, 20, 100, and 500 μM) and drought stress was simulated with 5% polyethylene glycol 6000 (PEG6000). The results showed that 100 μM melatonin can… More >

  • Open Access

    REVIEW

    The Genetic and Biochemical Mechanisms Underlying Cereal Seed Dormancy

    Sasa Jing1, Yuan Tian1, Heng Zhang2, John T. Hancock3, Ying Zhu2,*, Ping Li1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1203-1214, 2023, DOI:10.32604/phyton.2023.026305

    Abstract The crop seeds have been a staple food for humans, and seed yield is important for sustaining agriculture development and enhancing human adaptability to food risks. The phenomenon of pre-harvest sprouting (PHS), caused by seed dormancy deficiency, and the phenomenon of low seedling emergence caused by seed deep dormancy, will lead to a reduction in agricultural production. Therefore, it is particularly important to understand the regulation mechanisms of seed dormancy. There are many studies on the regulation of seed dormancy in rice, but there are few studies on the regulation of seed dormancy in other More >

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