Miravalles MT¹, MN Fioretti¹, H Mirassón², ML Faraldo², L Möck¹, RE Brevedan¹

Phyton-International Journal of Experimental Botany, Vol.82, pp. 263-273, 2013, DOI:10.32604/phyton.2013.82.263

Abstract In the present study the effects of three tillage systems: conventional (CT), vertical (VT) and direct drilling (DD) on three bread wheat varieties of different cycle length, were studied, for yield and yield components. Their influence on grain quality, expressed through thousand kernel weight (TKW, g), test weight (TW, kg/hL), grain protein content (GPC, %) and yellowberry percentage (YBP, %) were also studied. The highest average yield was under CT and DD, while the lowest was under VT. The lowest dry matter accumulation and one third fewer spikes per unit area were determined under VT. Concerning grain quality, a comparatively… More >

Castro Nava S

Phyton-International Journal of Experimental Botany, Vol.82, pp. 249-254, 2013, DOI:10.32604/phyton.2013.82.249

Abstract Identification of plant tolerance to heat requires novel strategies for measuring plant responses to this stress. Leaf electrolyte leakage is an indirect method that can be utilized as selection criteria for heat tolerance in plants, but it has to be adapted to each species. The objectives of this study were to measure heat-induced leaf electrolyte leakage in corn and bean to determine heat tolerant genotypes, and also to establish the optimal developmental stage for use of electrolyte leakage as selection criteria. This study included two genotypes of corn (Zea mays L.), and other two of bean (Phaseolus vulgaris L.), measured… More >

He XY^1,2, H Ye¹, JL Ma^1,2, RQ Zhang², GC Chen¹, YY Xia¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 177-180, 2012, DOI:10.32604/phyton.2012.81.177

Abstract Annual leaf segments of eight Camellia species were used to study the heat tolerance by an electrical conductivity method, in combination with a Logistic equation to ascertain the semi-lethal high temperature by fitting the cell injury rate curve. The relationship between the processing temperature and the cell injury rate in Camellia showed a typical "S" shaped curve, following the Logistic model. The correlation coefficient was above 0.95. The semi-lethal high temperature LT₅₀ of the eight Camellia species, determined by the inflection point on the curve, varied from 50 to 57 LT₅₀ / °C, following the descending order: Camellia oleifera >… More >

Simonetti E¹, E Alba², A Delibes²

Phyton-International Journal of Experimental Botany, Vol.81, pp. 139-142, 2012, DOI:10.32604/phyton.2012.81.139

Abstract In a previous work, deduced amino acid sequences from twenty wheat peroxidase genes were assigned to seven groups designated as TaPrx108 to TaPrx114. Some of these apoplastic peroxidases have previously shown to play different roles in the plant defense responses to infection by the cereal cyst nematode Heterodera avenae. In the present study, PCR marker analysis using Sears’s aneuploid wheat lines cv. ‘Chinese Spring’ was used to locate four genes encoding peroxidase isozymes. The TaPrx111-A, TaPrx112-D and TaPrx113-F genes were located on the short arm of chromosome 2B and the TaPrx109-C on the long arm of chromosome 1B. These results… More >

Hernández-León R¹, M Martínez-Trujillo², E Valencia-Cantero¹, G Santoyo¹

Phyton-International Journal of Experimental Botany, Vol.81, pp. 133-137, 2012, DOI:10.32604/phyton.2012.81.133

Abstract Rhizospheric soil of wheat plants contains a high diversity of microorganisms, and therefore, comprises a large reservoir for discovering genes with diverse agro-biotechnological applications. In this work, we constructed an E. coli metagenomic library based on bacterial artificial chromosome (BAC) clones with large genomic inserts from metagenomic DNA from the rhizosphere of wheat plants. The average of the DNA cloned segments varies from 5 to 80 kb, with an average size of 38 kb. Random clones were end-sequenced and homology results showed that the clonation of metagenomic DNA codes mainly for metabolic and catalytic functions (40%), including amidohydrolase, hydrolase, peptidase,… More >

Velázquez-Sepúlveda I, MC Orozco-Mosqueda, CM Prieto-Barajas, G Santoyo

Phyton-International Journal of Experimental Botany, Vol.81, pp. 81-87, 2012, DOI:10.32604/phyton.2012.81.081

Abstract Rhizospheric soil is one the largest reservoirs of microbial genetic diversity. Before conducting a large-scale metagenomic analysis of an environment, such as a rhizospheric soil, it is necessary to perform a pre-screening of the resident genetic diversity. In this study, we analyzed the bacterial diversity associated with the rhizosphere of wheat plants by PCR amplification, construction of a library and sequencing of 16S rDNA genes. Thirty OTUs were detected, including the Classes Alfaproteobacteria, Betaproteobacteria, Deltaproteobacteria, Gammaproteobateria, Actinobacteria, Bacilli, Clostridia and Uncultivable bacteria. Within the Gammaproteobacteria class, the genera Pseudomonas, Stenotrophomonas and Bacillus were the most abundant, since they corresponded to… More >

Morant AE, HD Merchán, EE Lutz

Phyton-International Journal of Experimental Botany, Vol.80, pp. 113-117, 2011, DOI:10.32604/phyton.2011.80.113

Abstract The wheat vernalization response is controlled by three recessive alleles identified and located, that allows to characterize varieties through genetic analysis. However, it is unknown whether the same genetic constitution at a loci scale in different varieties implies the same (1) requirement of cold hours, and (2) interaction with the photoperiod for induction. Therefore, we conducted an experiment with varieties carrying the alleles (vrn-A1, vrn-B1 vrn-D1), extreme condition of vernalization requirements, to compare the duration of the vegetative phase under different photoperiods. The study was conducted in a greenhouse with four varieties of double purpose wheat. The treatments were 8,… More >

Jurado E¹, M Márquez-Linares², J Flores³

Phyton-International Journal of Experimental Botany, Vol.80, pp. 101-105, 2011, DOI:10.32604/phyton.2011.80.101

Abstract We evaluated the effect of cold storage and fire-related cues on seed germination of Arctostaphylos pungens HBK (Mexican Manzanita), a common shrub in poorly managed pine-oak forests in Durango, Mexico. Because this shrub has a high density in previously burnt forests, we investigated the effect that high temperatures, smoke and charcoal might have on seed germination of this species. Seeds were collected fresh from the shrubs. The highest germination was 30% for seeds that had been cold stored, heat shocked, past through smoke and watered with diluted charcoal. More >

Morant AE, HD Merchán, EE Lutz

Phyton-International Journal of Experimental Botany, Vol.80, pp. 5-8, 2011, DOI:10.32604/phyton.2011.80.005

Abstract This study aimed to characterize wheat cultivars for their chilling requirements for differentiation and their relationship to flowering time, to establish a dual purpose ideotype. Seeds of eight genotypes (cultivars and advanced lines) of bread wheat were exposed to constant temperature (4 °C) in germination chambers for 8, 6, 4 and 2 weeks. A control treatment was kept at room temperature. Four replicates of each treatment were placed in the greenhouse in 250 cc pots containing 4 seedlings each, with irrigation and natural photoperiod, following a randomized complete block design. The greenhouse temperature was constant (20 °C) and the natural… More >

Mirassón¹ HR, ML Faraldo¹, MN Fioretti², M Miravalles², RE Brevedan²

Phyton-International Journal of Experimental Botany, Vol.79, pp. 183-187, 2010, DOI:10.32604/phyton.2010.79.183

Abstract Tillage system is an important management decision that has to be taken in the central, semiarid, temperate region of Argentina for dryland wheat production. A 2-year study was conducted on an Entic Haplustoll soil with a sandy loam texture to determine the effects of three tillage systems (conventional, vertical and notillage) on leaf water status and chlorophyll content. Measurements were made at the Z65 growth stage with a SPAD-502; leaf water potential measurements were simultaneously taken. There was a positive relationship between leaf water potential and leaf greenness in both years. Tillage systems were significantly different in 2007; both variables… More >