Shengzhe Ma^1,#, Xiaoming Zhu^1,#, Cheng Xin^1,#, Fuao Cao¹, Mengnan Xu¹, Xiangling Han¹, Jinke Sui^3,*, Wenjun Chang^2,*, Wei Zhang^1,*

Oncologie, Vol.24, No.4, pp. 823-833, 2022, DOI:10.32604/oncologie.2022.025411

Abstract Background: Reticulocalbin 3 (RCN3) has been associated with several malignancies. However, its role in colorectal cancer (CRC) remains controversial. Thus, this study aimed to investigate the role of RCN3 in CRC prognosis. Methods: The clinical significance of RCN3 expression in CRC was evaluated in a large cohort of 483 patients. Normal tissues, carcinoma, para-carcinoma, adenoma, and metastatic tissues were evaluated by immunohistochemistry. We investigated the association between RCN3 expression and CRC occurrence in tumors and other tissues. Prognostic factors were also evaluated by Kaplan-Meier survival analysis and the Cox regression model. Results: RCN3 was significantly overexpressed in CRC and metastatic… More >

OSCAR MAC DONAL¹, JUAN G. CHEDIACK^1,2,3, ENRIQUE CAVIEDES-VIDAL^1,2,3

BIOCELL, Vol.32, No.3, pp. 219-227, 2008, DOI:10.32604/biocell.2008.32.219

Abstract The isolation of viable enterocytes, villi and crypts from the small intestine of a feral bird (Columba livia) is important for performing physiological experiments in ecologically relevant processes of membrane transport. The effectiveness of mechanical disruption, enzymatic digestion and chelating agents were compared. The objectives were to isolate enterocytes, villi and crypts from the small intestine of young pigeons; to evaluate the viability of the isolated intestinal epithelial cells isolated; and to verify the integrity of enterocytes by biochemical features. Enzymatic and mechanical methods yielded both elongated columnar and spherical cells. With the chelating method villi and crypts were obtained.… More >

MARIA RAQUEL MARÇAL NATALI, SONIA LUCY MOLINARI, LUIZ CRISTIANO VALENTINI, MARCÍLIO HUBNER DE MIRANDA NETO.

BIOCELL, Vol.29, No.1, pp. 39-46, 2005, DOI:10.32604/biocell.2005.29.039

Abstract The purpose of this work was to analyze the morphoquantitative behavior of the neurons of the myenteric plexus, as well as the morphometry of the duodenal wall, in adult rats fed with normoproteic (22%) and hypoproteic (8%) rations, killed at the age of 345 days. For neuronal assessments duodenal wholemounts stained with the Giemsa method were used, and for the evaluation of the duodenal wall routine histological processing and staining with Hematoxilin-Eosin were employed. The means of the number of neurons in 80 microscopic fields (12.72 mm² ) and of the size of the neuronal cell bodies did not reveal… More >

NILZA CRISTINA BUTTOW, MIRIAM SANTIN, LUCIANA CONCI MACEDO, ALINE CRISTINA NERES TEIXEIRA, GISELE CAROLINE NOVAKOWSKI, TAÍSE ROBERTA BOLONHEIS ARMELIN, KATHYA ASSMANN

BIOCELL, Vol.28, No.2, pp. 135-142, 2004, DOI:10.32604/biocell.2004.28.135

Abstract A morphological and quantitative study in the ileal and colonic myenteric and submucous plexuses of rats after BAC denervation was performed. Four groups were employed: SI – ileum control; CBI – denervated ileum; SC – colon control; and CBC – denervated colon. We used the Myosin-V immunohistochemistry technique to study the myenteric and submucous plexuses. In the submucous plexus of the ileum and colon there was not a significant decrease in the number of neurons/mm2 and of ganglia/mm2 . The denervation of the myenteric plexus in the group CBI was 44.7% and in the group CBC, 68.3%. In the myenteric… More >

LUZMARINA HERNANDES*, LUCIENI CRISTINA MARQUES DA SILVA PEREIRA*, ELIANA PARISI ALVARES**

BIOCELL, Vol.27, No.3, pp. 347-351, 2003, DOI:10.32604/biocell.2003.27.347

Abstract The enteric nervous system plays a role on the stimulation of secretory cells of intestinal epithelia. We have demonstrated that ablation of ENS stimulates epithelial cell proliferation. As goblet cells are important constituents of the epithelial sheet, it is mandatory to investigate separately this cell type. The myenteric plexus of the ileum of rats in postnatal development was partially removed by the serosal application of benzalkonium chloride (BAC). Three groups of animals were used: those where BAC application was at 13 days and sacrifice was at 15 (13/28-day-old) or 23 days after treatment (13/36-day-old), and those where BAC was applied… More >

Erik B. Kistler¹, Geert W. Schmid-Schönbein^2,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 8-8, 2019, DOI:10.32604/mcb.2019.07256

Abstract A longstanding question in research on organ failure after physiological shock (such as trauma, burns, sepsis, surgery and medical emergencies) is the underlying mechanism for a progressive loss of cell and tissue functions. Our systematic analysis of this problem has served to identify digestive enzymes as key players [1, 2]. After synthesis and discharge from the pancreas, the digestive enzymes are usually contained inside the lumen of the small intestine where they break down food every day. Escape of the digestive enzymes out of the lumen of the intestine is kept to a minimum by the mucosal barrier in the… More >

H. Gregersen¹, G.S. Kassab², Y.C. Fung²

Molecular & Cellular Biomechanics, Vol.1, No.3, pp. 191-200, 2004, DOI:10.3970/mcb.2004.001.191

Abstract During the last decades, it has become increasingly common to make balloons distension in visceral organs in vivo. In particular this is true for studies of gastrointestinal motor function and biomechanics. Balloon distension is often used for assessment of small intestinal compliance and tension based on Laplace's law for cylindrical pressure pipes. This commonly used law is valid only when the balloon-distended intestine is cylindrical. Experimentally, it is seen that the diameter of the balloon-distended intestine is not a constant, but variable in the axial direction. Hence, it is necessary to improve Laplace's law for intestinal investigation. In this paper… More >

Odin RAMÍREZ-FERNÁNDEZ^1,2,3,*, Ludovika CACOPARDO¹, Benjamín LEON-MANCILLA², Joana COSTA¹

BIOCELL, Vol.43, No.1, pp. 29-36, 2019, DOI:10.32604/biocell.2019.04790

Abstract We present a bioreactor system which combines a semi permeable membrane that simulates the osmotic nutrients interchange in the small intestine circulation and rhythmic peristaltic movement. This custom-designed presents a semipermeable membrane bioreactor, with peristaltic flow and compression variation that allows adjustment of luminal flow rate. In addition, this system is also capable of achieving the drug distribution in the small intestine model from the apical compartment to the basal compartment by the semipermeable channel. This dynamic bioreactor can mimic the human small intestine with increased accuracy to overcome many of the limitations and accuracy with the previously described in… More >