Carmona MA¹, E Simonetti^2,3, ME Ravotti¹, MM Sc,iani⁴, AG Luque⁴, NA Formento⁵, FJ Sautua¹

Phyton-International Journal of Experimental Botany, Vol.86, pp. 265-269, 2017, DOI:10.32604/phyton.2017.86.265

Abstract Soybean root and stem rots caused by soil-borne pathogens are diseases commonly found in soybean fields, and one of the most important causes of crop losses. In the present study, the mycelial sensitivity of Fusarium virguliforme, F. tucumaniae, Sclerotinia sclerotiorum and Macrophomina phaseolina was evaluated on potato dextrose agar media (25 mL) supplemented with different concentrations of manganese phosphite (MnPhi) diluted in water (0, 25, 37.5, 50, 100, 200, 300, 400, 500, 800 and 1000 μg/mL). Mycelial growth sensitivity was analyzed using logarithmic linear regression analysis. The MnPhi concentration needed to inhibit 50% of the mycelial growth (IC₅₀) ranged from… More >

García-Munguía CA¹, M Acosta-Ramos², O Vázquez-Martinez³, M Pérez-Sato⁵, A García-Munguía⁴, O García-Munguía⁶, AM García-Munguía³

Phyton-International Journal of Experimental Botany, Vol.86, pp. 258-264, 2017, DOI:10.32604/phyton.2017.86.258

Abstract The illnesses that cause root and neck rot in golf courses constitute a limiting factor for grass development worldwide. This is mainly true when management guidelines are not conducted. Our research was conducted in the Golf Club “Mexico”, located in Mexico, D.F. Isolates were taken from plants that presented yellowing symptoms and root and neck rot. The identified pathogenic fungi, isolated from axenic media, were Giberella avenacea, Fusarium oxysporum, F. oxysporum f. sp. cubense and Pythium ultimum. The molecular characterization showed a comparison of the DNA-obtained sequences which was morphologically determined as F. avenaceum and was molecularly corroborated as G.… More >

Juan Agustín CUETO³, Emile SANTOS BARRIAS⁶, Wanderley de SOUZA^{4, 5}, Patricia Silvia ROMANO^{1, 2}

BIOCELL, Vol.42, No.3, pp. 105-108, 2018, DOI:10.32604/biocell.2018.07017

Abstract Trypanosoma cruzi is the causative agent of Chagas disease. This parasite requires the intracellular niche in order to proliferate and disseminate the infection. After invasion, T. cruzi resides temporarily in an acidic vacuole which is lysed by a not well-understood mechanism. Transmission electron microscopy was used to describe the process of T. cruzi escape from the parasitophorous vacuole over the time. Using HeLa (non-professional phagocytic cells) as host cell, we observed that recently internalized parasites reside in a membrane-bounded vacuole. A few hours later, the first sign of vacuole disruption appeared as membrane discontinuities. This observation was followed by a… More >

Mahmoud MOUSTAFA^{1, 2}, Saad ALAMRI¹, Mohamed ELNOUBY³, Tarek TAHA⁴, M. A. ABU-SAIED⁵, Ali SHATI¹, Mohamed AL-KAHTANI¹, Sulaiman ALRUMMAN¹

BIOCELL, Vol.42, No.3, pp. 93-98, 2018, DOI:10.32604/biocell.2018.07014

Abstract Water contaminated with pathogenic microbes is considered as one of the most common routes for transmitting diseases in human beings. Different methods have been applied for the decontamination of microbes in contaminated water. In the current study, an easy to do hydrothermal method has been used for the preparation of TiO₂-Ag nanoparticles. The obtained material was characterised using a scanning electron microscope (SEM) and fourier transform infra-red spectroscopy (FTIR). The morphological appearance of the obtained nanoparticles was in the shape of a sphere with a size range of 60-90 nm. The antimicrobial activity of the prepared nanoparticles was tested against… More >

Hernandez-Trejo A¹, B Estrada-Drouaillet¹, JA López-Santillán¹, C Rios-Velasco², SE Varela-Fuentes¹, R Rodríguez-Herrera³, E Osorio-Hernández¹

Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 47-54, 2019, DOI:10.32604/phyton.2019.04611

Abstract The control of Spodoptera frugiperda is based on synthetic insecticides, so some alternatives are the use of entomopathogenic fungi (EF) and neem extract. The objective of the study was to evaluate in vitro effectiveness of native EF and neem extracts on S. frugiperda larvae. Six EF were identified by DNA sequencing of ITS regions from three EF (Fusarium solani, Metarrhizium robertsii, Nigrospora spherica and Penicillium citrinum). They were evaluated in concentrations of 1 × 10⁸ spores/ mL. In addition, a second bioassay was carried out evaluating only F. solani, M. robertsii and N. sphaerica and the addition of vegetable oil.… More >

Hernández Pérez A¹, E Cerna Chávez², JC Delgado Ortiz³, M Beltrán Beache⁴, LM Tapia Vargas⁵, YM Ochoa Fuentes²

Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 11-13, 2019, DOI:10.32604/phyton.2019.04608

Abstract Mexico is the main producer, consumer and exporter of avocado in the world, being Michoacan the main producer state contributing more than 80% of the national production. There are phytopathogens that decimate the production causing the death of the tree. Root samples were collected in avocado trees that showed the characteristic symptomatology of the disease known as avocado sadness, the sampling was carried out in four of the main avocado producing towns, in the state of Michoacan, Mexico. The isolation consisted in sowing root tissue in Petri dishes with V8^®-PARPH culture medium, subsequently they were identified morphologically and for species… More >

Angela Reynolds^∗,†, Kittisak Koombua^‡, Ramana M. Pidaparti^{†,‡, §}, Kevin R. Ward^†,¶

Molecular & Cellular Biomechanics, Vol.9, No.2, pp. 141-156, 2012, DOI:10.3970/mcb.2012.009.141

Abstract Better understanding of the acute/chronic inflammation in airways is very important in order to avoid lung injuries for patients undergoing mechanical ventilation for treatment of respiratory problems. Local lung inflammation is triggered by many mechanisms within the lung, including pathogens. In this study, a cellular automata based model (CA) for pulmonary inflammation that incorporates biophysical processes during inflammatory responses was developed. The developed CA results in three possible outcomes related to homeostasis (healing), persistent infection, and resolved infection with high inflammation (inflamed state). The results from the model are validated qualitatively against other existing computational models. A sensitivity analysis was… More >