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  • Open Access

    ARTICLE

    Global and Comparative Proteome Analysis of Nitrogen-Stress Responsive Proteins in the Root, Stem and Leaf of Brassica napus

    Liang Chai1,2, Cheng Cui1, Benchuan Zheng1, Jinfang Zhang1, Jun Jiang1, Haojie Li1,2,*, Liangcai Jiang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 645-663, 2023, DOI:10.32604/phyton.2023.024717

    Abstract Nitrogen (N) is one of the basic nutrients and signals for plant development and deficiency of it would always limit the productions of crops in the field. Quantitative research on expression of N-stress responsive proteins on a proteome level remains elusive. In order to gain a deep insight into the proteins responding to nitrogen stress in rapeseed (Brassica napus L.), comparative proteomic analysis was performed to investigate changes of protein expression profiles from the root, stem and leaf under different N concentrations, respectively. More than 200 differential abundance proteins (DAPs) were detected and categorized into groups according to annotations, including… More >

  • Open Access

    ARTICLE

    Proteomic Analysis of Chrysanthemum Lateral Buds after Removing Apical Dominance Based on Label-Free Technology

    Sicong Zheng#, Jingjing Song#, Cheng Luo, Xin Li, Qiqi Ma, Beibei Jiang*, Qinglin Liu, Yuanzhi Pan

    Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 525-539, 2022, DOI:10.32604/phyton.2022.017629

    Abstract Studying the genetic basis and regulatory mechanism of chrysanthemum lateral bud outgrowth is of great significance for reduction the production cost of cut chrysanthemum. To clarify the molecular basis of lateral bud elongation after removal of apical dominance in chrysanthemum, label-free quantification analysis was used to analyze the proteome changes after apical bud removal. Quantitative real-time PCR (qPCR) was used to analyze the changes in the expression of three plant hormone-related genes. A total of 440 differentially expressed proteins were successfully identified at three time points during the lateral bud elongation. The number of differentially expressed proteins in the three… More >

  • Open Access

    ARTICLE

    Proteome-wide screening for the analysis of protein targeting of Chlamydia pneumoniae in endoplasmic reticulum of host cells and their possible implication in lung cancer development

    YANYAN LI1, SHAHANAVAJ KHAN2,3,4, ANIS AHMAD CHAUDHARY5, HASSAN AHMED RUDAYNI5, ABDUL MALIK2, ASHWAG SHAMI6

    BIOCELL, Vol.46, No.1, pp. 87-95, 2022, DOI:10.32604/biocell.2022.016509

    Abstract Available reports have confirmed a link between bacterial infection and the progression of different types of cancers, including colon, lungs, and prostate cancer. Here we report the Chlamydia pneumonia proteins targeting in endoplasmic reticulum (ER) using in-silico approaches and their possible role in lung cancer etiology. We predicted 48 proteins that target human ER, which may be associated with protein folding and protein-protein interactions during infection. The results showed C. pneumoniae proteins targeting human ER and their implications in lung cancer growth. These targeted proteins may be involved in competitive interactions between host and bacterial proteins, which may change the… More >

  • Open Access

    ARTICLE

    Data-independent acquisition mass spectrometry quantitative proteomic analysis reveals that skin aging-related proteins differ between men and women

    HUI ZHANG1,3,#, SHIRUI CHEN1,3,#, MENGTING LIU1,2, YAOCHI WANG1,2, CONG XIN1,2, JING MA1,3, XIAODONG ZHENG1,2, YUANDI HUANG1,2, BO ZHANG3,4, XUEJUN ZHANG1,2, LIANGDAN SUN1,2,*, SEN YANG1,3,*

    BIOCELL, Vol.45, No.5, pp. 1321-1335, 2021, DOI:10.32604/biocell.2021.016524

    Abstract The skin is the largest organ of the human body, and its aging is visible to the naked eye. The aging rate of men and women is slightly different. This study compared the protein expression of skin samples on the curved forearms of 11 healthy women and 9 healthy men. Quantitative proteomics analysis found that the expression of epidermal proteins in men and women of the same age group was different. Compared with female skin, in male skin, 20 proteins were upregulated, and 7 proteins were downregulated. These data suggest that men and women have differences in the speed of… More >

  • Open Access

    ARTICLE

    Comparative proteome analysis in hot pepper (Capsicum annuum L.) after space flight

    Xie LB1, X Wang1, M Peng2, Y Zhou1, LX Chen1, LX Liu3, YL Gao1, YH Guo1

    Phyton-International Journal of Experimental Botany, Vol.86, pp. 236-245, 2017, DOI:10.32604/phyton.2017.86.236

    Abstract Hot pepper (Capsicum annuum L.) is an important crop all over the world. To explore and identify differentially expressed proteins of hot pepper after space flight, three space-induced mutants (Y1, Y2 and Y3), which obtained new traits after space flight compared with their control lines (W1 and W2), were analyzed using comparative proteome analysis. In this study, leaf morphological characteristics of five kinds of hot pepper variations were evaluated by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Results showed that obvious changes of cellular structure were seen in space induced mutants. Thirty nine out of the 255 differentially… More >

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