CHUNKUN FAN, YONGPENG ZHANG, CHUNBAO YANG, YAWEI TANG, JI QU, BU JIE, DEJI QUZHEN, LIYUN GAO^*

BIOCELL, Vol.45, No.4, pp. 1121-1135, 2021, DOI:10.32604/biocell.2021.014336

Abstract Salinity is a serious challenge for agriculture production by limiting the arable land. Rice is a major staple food crop but very sensitive to salt stress. In this study, we used Arabidopsis for the functional characterization of a rice F-box gene LOC_Os04g48270 (OsPP12-A13) under salinity stress. OsPP12-A13 is a nuclear-localized protein that is strongly upregulated under salinity stress in rice and showed the highest expression in the stem, followed by roots and leaves. Two types of transgenic lines for OsPP12-A13 were generated, including constitutive tissue over-expression using the CaMV35S promoter and phloem specific over-expression using the pSUC2 promoter. Both types… More >

ZIXUAN WANG^1,#, HONGMEI PENG^2,#, WEI SHI^4,#, LU GAN¹, LIPING ZHONG¹, JIAN HE¹, LINLIN XIE¹, PAN WU¹, YONGXIANG ZHAO¹, ZHIMING DENG^3,*, HONGLIANG TANG⁴, YONG HUANG^1,3,*

BIOCELL, Vol.45, No.3, pp. 489-500, 2021, DOI:10.32604/biocell.2021.014439

Abstract Although great achievements have been made in the past decades in medicine, cancer remains a worldwide public health issue. Surgery is usually accompanied by shortcomings such as residual lesions and poor treatment effects, and the successive appearance of other treatment methods, such as radiotherapy and chemotherapy, has not changed the postoperative recurrence rate, toxicity, and side effects. However, the advent of photodynamic therapy has greatly improved this situation. Photodynamic therapy is an emerging tumor diagnosis and treatment technology with good application prospects, photodynamic therapy uses a specific wavelength of light to excite a photosensitizer to generate reactive oxygen species, damage… More >

Abdul Awal Chowdhury Masud¹, Md. Fazlul Karim¹, M. H. M. Borhannuddin Bhuyan², Jubayer Al Mahmud³, Kamrun Nahar⁴, Masayuki Fujita⁵, Mirza Hasanuzzaman^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 353-372, 2021, DOI:10.32604/phyton.2021.013259

Abstract Drought is the most common form of abiotic stress that reduces plant growth and productivity. It causes plant injuries through elevated production of reactive oxygen species (ROS). Potassium (K) is a vital plant nutrient that notably ameliorates the detrimental effect of drought stress in the plant. A pot experiment was conducted at the Laboratory of Plant Stress Responses, Faculty of Agriculture, Kagawa University, Japan, under controlled environment of green house to explore the role of K in mitigating drought severity in wheat (Triticum asevitum L.) seedlings. Three days after germination, seedlings were exposed to three water regimes viz., 100, 50,… More >

Hailin Shi^#, Shiwei Sun^#, Xiaohong Liu, Jiahe Fan, Jin Wang, Ke Zhao, Wei Wang^*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 159-170, 2021, DOI:10.32604/phyton.2020.011620

Abstract Allelopathic plants are important resources for the discovery of bioherbicides. Rosebay willowherb [Chamaenerion angustifolium (L.) Scop. syn. Epilobium angustifolium L.] widely distributes in Western Asia, Europe, and North America, and behaves as a dominant species within the community due to the production of substances that restrict growth of other plants. This study aims at investigating the allelopathic potential of rosebay willowherb by evaluation of the effects of aqueous extracts from different parts on seed germination and seedling growth in lettuce (Lactuca sativa L.), as well as measuring the accumulation of reactive oxygen species and structural analysis of root tips via… More >

Mahmuda Binte Monsur¹, Nasrin Akter Ivy¹, M. Moynul Haque², Mirza Hasanuzzaman³, Ayman EL Sabagh^4,5,*, Md. Motiar Rohman^6,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 497-517, 2020, DOI:10.32604/phyton.2020.09144

Abstract The research was conducted to investigate comparative oxidative damage including probable protective roles of antioxidant and glyoxalase systems in rice (Oryza sativa L.) seedlings under salinity stress. Seedlings of two rice genotypes: Pokkali (tolerant) and BRRI dhan28 (sensitive) were subjected to 8 dSm⁻¹ salinity stress for seven days in a hydroponic system. We observed significant variation between Pokkali and BRRI dhan28 in phenotypic, biochemical and molecular level under salinity stress. Carotenoid content, ion homeostasis, antioxidant enzymes, ascorbate and glutathione redox system and proline accumulation may help Pokkali to develop defense system during salinity stress. However, the activity antioxidant enzymes particularly… More >

Zhenzhen SHI, Jiaxin CAO, Hailong PANG, Lingyun JIA, Kun SUN, Hanqing FENG^*

BIOCELL, Vol.44, No.1, pp. 89-99, 2020, DOI:10.32604/biocell.2020.08059

Abstract Cadmium (Cd) is one of the most widespread and toxic heavy metals to plants. Extracellular ATP (exATP) is thought to be an extracellular effector in regulating the physiological responses of plant cells to environmental stresses. However, the function of exATP in Cd-stressed plant cells is much unknown. The present work showed that treating tobacco (Nicotiana tabacum L. cv. Bright Yellow-2) cell-suspension cultures with exogenous CdCl₂ reduced the cell viability, exATP level, and Mg content. However, the production of reactive oxygen species (ROS), Cd content, and electrolyte leakage of the cells were enhanced by exogenous CdCl₂. When the Cd-induced accumulation of… More >

G.C. DALVIT, P.D. CETICA, L.N. PINTOS, M.T. BECONI

BIOCELL, Vol.29, No.2, pp. 209-212, 2005, DOI:10.32604/biocell.2005.29.209

Abstract Oxidative modifications of cell components due to the action of reactive oxygen species (ROS) is one of the most potentially damaging processes for proper cell function. However, in the last few years it has been observed that ROS participate in physiological processes. The aim of this work was to determine ROS generation during in vitro production of bovine embryos. Cumulus-oocyte complexes were recovered by aspiration of antral follicles from ovaries obtained from slaughtered cows and cultured in medium 199 for 22 h at 39ºC in 5% CO₂: 95% humidified air. In vitro fertilization was carried out in IVF-mSOF with frozenthawed… More >

ELISA CEBRAL^1,2, ISABEL CARRASCO¹ , DAVID VANTMAN¹, ROSITA SMITH¹

BIOCELL, Vol.31, No.1, pp. 51-59, 2007, DOI:10.32604/biocell.2007.31.051

Abstract Exposure of either gametes or embryos to conditions and/or factors that generate oxidative stress has been associated with impaired early embryogenesis. The effects of reactive oxygen species (ROS) on mouse preimplantation development, depending of the ROS-concentration and time of exposition, were studied. Two-cell embryos were incubated with 5, 10, 25 and 50 μM of hydrogen peroxide (H₂O₂) for 30 and 60 minutes of exposition and allowed to develop for 72 h to study the quality of development. The incubation with 50 μM H₂O₂ for 30 or 60 minutes, strongly inhibited the 2-cell embryo development as compared to the control (p<0.001).… More >

Atif Kamil¹, Mubarak Ali Khan¹, Muhammad AAsim², Nadir Zaman Khan², Raham Sher Khan¹, Muhsin Jamal³, Waqar Ahmad⁴, Mir Azam Khan⁴, Fazal Jalil⁴

BIOCELL, Vol.43, No.3, pp. 167-174, 2019, DOI:10.32604/biocell.2019.06729

Abstract Several toxic compounds are known to induce apoptosis in mammalian cell lines. The human neuroblastoma cells (SH-SY5Y) were exposed to the phosphatase inhibiting toxin okadaic acid (OA) or hydrogen peroxide (H2O2) to induce apoptosis as well as generate reactive oxygen species (ROS). Mitoxantrone (MXT) was used as a positive control for apoptosis. The SH-SY5Y cells were transfected with eukaryotic expression plasmid pHyPer-dMito encoding mitochondrial-targeted fluorescent or pHyPer-dCito encoding cytoplasmic-targeted fluorescent sensor for hydrogen peroxide (HyPer). The ERp57, also called GRP58 (Glucose-regulated protein 58), is a stress protein induced in conditions like glucose starvation and viral infection. Recently ERp57 was shown… More >