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A Novel Dominant Allele from 93-11, ES(4), Represses Reactive Oxygen Species Scavenging and Leads to Early-Senescence in Rice

Zhishu Jiang#, Cong Gan#, Yulian Liu, Xiaoli Lin, Limei Peng, Yongping Song, Xiaowei Luo, Jie Xu*
Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang, 330045, China
* Corresponding Author: Jie Xu. Email:
(This article belongs to this Special Issue: High-Yield Rice Physiology & Genetics)

Phyton-International Journal of Experimental Botany 2023, 92(3), 665-677. https://doi.org/10.32604/phyton.2023.025266

Received 02 July 2022; Accepted 25 August 2022; Issue published 29 November 2022

Abstract

Senescence is the last developmental process in plant, which has an important impact on crop yield and quality. In this study, a stable hereditary early-senescence line BC64 was isolated from the high-generation recombinant inbred lines of 93-11 and Wuyunjing7 (W7). Genetic analysis showed that the premature aging phenotype was controlled by a dominant gene derived from 93-11. By linkage analysis, the gene was primarily mapped in the region between marker B4 and B5 near the centromere of chromosome 4, described as ES(4). Through multiple backcrossing with W7, the near-isogenic line of ES(4) (NIL-ES(4)) was obtained. Compared with wild-type W7, NIL-ES(4) plants showed more sever senescence phenotype in both nature and dark conditions. In NIL plants, leaves turned yellow at the fully tillering stage; photosynthetic rate, pollen fertility and seed setting rate were decreased. Moreover, the malondialdehyde, proline content and relative conductivity in NIL-ES(4) were significantly higher than those in W7; both transcript level and activities of reactive oxygen species scavenging enzymes were repressed; H2O2 and O2− were significantly accumulated. This study provides a basis for further cloning and function identification of ES(4).

Keywords

Rice; early-senescence; gene mapping; chlorophyll degradation; reactive oxygen species scavenging

Cite This Article

Jiang, Z., Gan, C., Liu, Y., Lin, X., Peng, L. et al. (2023). A Novel Dominant Allele from 93-11, ES(4), Represses Reactive Oxygen Species Scavenging and Leads to Early-Senescence in Rice. Phyton-International Journal of Experimental Botany, 92(3), 665–677.



This work is licensed under a Creative Commons Attribution 4.0 International License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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