Sugey Vásquez-Hernández, Joaquín Adolfo Montes-Molina^*, Federico Antonio Gutiérrez-Miceli, Sheila Jazmín Reyes-Zambrano, Carlos Alberto Lecona-Guzmán^*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2065-2078, 2023, DOI:10.32604/phyton.2023.028784

Abstract Biotechnological techniques provide a viable alternative to help improve and increase the production of plant species of agricultural and economic importance, which have been affected over the years by climate change, increasing their susceptibility to pests and/or diseases, generating losses in production as well as a decrease in their regenerative and genetic diversity. The application of biotechnological techniques such as in vitro mutagenesis offers a viable option for the generation of crops that are resistant to the different factors caused by abiotic and biotic stress. In vitro mutagenesis has been used in an efficient way to generate genetic changes in… More >

Shaochun Liu, Jiamin Hu, Haohua He, Junru Fu, Xu Jie, Dahu Zhou^*, Haihui Fu^*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2035-2049, 2023, DOI:10.32604/phyton.2023.028296

Abstract OsPLS4 encodes a β-ketoacyl carrier protein reductase (KAR). The role of OsPLS4 in rice sheath blight (Rhizoctonia solani) remains unclear. Our preliminary studies showed that premature leaf senescence mutants (pls4) were highly susceptive to sheath blight in the early stage of rice development. To explore the role of this gene in the development of rice sheath blight, the transcriptome profiles of the rice pls4 mutant and wild type were compared by RNA-seq. The results revealed 2,569 differentially expressed genes (DEGs). The down-regulated genes were significantly enriched in the defense response-related biological processes. These down-regulated genes included the chitinase genes and… More >

TAO LI^#, XIN FU^#, JIE WANG, WEI SHANG, XIAOTONG WANG, LINYUN ZHANG, JUN LI^*

Oncology Research, Vol.31, No.3, pp. 345-359, 2023, DOI:10.32604/or.2023.028724

Abstract Temozolomide (TMZ) resistance is a major obstacle in glioma treatment. Nuclear protein-1 (NUPR1) is a regulator of glioma progression. This study investigated the mechanism of NUPR1 in TMZ resistance in hypoxia-treated glioma cells and its mechanism in modulating autophagy. We treated TMZ-resistant cells U251-TMZ and T98G-TMZ to normoxia or hypoxia and silenced NUPR1 in hypoxia-treated U251-TMZ and T98G-TMZ cells to assess cell viability, proliferation, apoptosis, LC3-II/LC3-I and p62 expressions, and autophagic flux under different concentrations of TMZ. We found that hypoxia upregulated NUPR1 expression and autophagy while NUPR1 silencing suppressed hypoxia-induced TMZ resistance and autophagy in glioma cells. We also… More > Graphic Abstract

CHANGMEI LONG, TINGTING YANG, YUJIE HAN, LIZHEN HAN^*

BIOCELL, Vol.47, No.6, pp. 1417-1430, 2023, DOI:10.32604/biocell.2023.027485

Abstract Background: The plant-growth-promoting rhizobacterium Tsukamurella tyrosinosolvens is a rare strain of actinomycete, in order to recognize and expand the ecological functions of rare actinomycetes. Methods: In this experiment, we studied the effect of Tsukamurella tyrosinosolvens P9 on the drought resistance of peanut by inoculating peanut seedlings in pots and measuring the growth and physiological indicators of peanut under drought stress and re-watering conditions. Results: The results showed that during drought stress, the relative water content of the soil and leaves, chlorophyll content, and stomatal length, width, and aperture were significantly decreased while the levels of malondialdehyde (MDA), H₂O₂ and stomatal… More >

Xintong Chen, Pinghua Zhu^*, Xiancui Yan, Lei Yang, Huayu Wang

Journal of Renewable Materials, Vol.11, No.6, pp. 2953-2967, 2023, DOI:10.32604/jrm.2023.027140

Abstract With the emphasis on environmental issues, the recycling of waste concrete, even recycled concrete, has become a hot spot in the field of architecture. But the repeated recycling of waste concrete used in harsh environments is still a complex problem. This paper discusses the durability and recyclability of recycled aggregate concrete (RAC) as a prefabricated material in the harsh environment, the effect of high-temperature curing (60°C, 80°C, and 100°C) on the frost resistance of RAC and physical properties of the second generation recycled coarse aggregate (RCA₂) of RAC after 300 freeze-thaw cycles were studied. The frost resistance of RAC was… More >

Ameer Abed Jaddoa^* , Hussain Saad Abd

Frontiers in Heat and Mass Transfer, Vol.20, pp. 1-12, 2023, DOI:10.5098/hmt.20.6

Abstract In this work, the demeanour of heat transfer in a cooled upstanding turbulent flow condition of supercritical carbon dioxide SC- CO2 was investigated and analyzed. Several scenarios were adopted to handle the experimental data acquired by applying a perpendicular pipe with bending tape used in the examination model. The outcomes were presented using a typical method 'the dimensionless constitution'. Also, a modification was achieved to improve the relationship among the parameters for the processes of up and down flows. The achieved results will help address this research gap on turbulent perpendicular compound heat load based on cooling conditions. Finally, the… More >

Yeyao Du^1,#, Ye Zhang^2,#, Yang Song¹, Zhuo Zhang¹, Sujie Fan¹, Hanzhu Zhang¹, Piwu Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1907-1920, 2023, DOI:10.32604/phyton.2023.027607

Abstract Soybean (Glycine max (Linn.) Merr.) annual leguminous crop is cultivated all over the world. The occurrence of diseases has a great impact on the yield and quality of soybean. In this study, based on the RNA-seq of soybean variety M18, a complete CDS (Coding sequence) GmPR1L of the pathogenesis-related protein 1 family was obtained, which has the ability to resist fungal diseases. The overexpression vector and interference expression vector were transferred into tobacco NC89, and the resistance of transgenic tobacco (Nicotiana tabacum L.) to Botrytis cinerea infection was identified. The results show that: Compared with the control, the activities of… More >

TABEA GEWALT^1,2, KA-WON NOH³, LYDIA MEDER^1,2,4,*

Oncology Research, Vol.31, No.2, pp. 101-115, 2023, DOI:10.32604/or.2023.028105

Abstract LIN28B is an RNA-binding protein that targets a broad range of microRNAs and modulates their maturation and activity. Under normal conditions, LIN28B is exclusively expressed in embryogenic stem cells, blocking differentiation and promoting proliferation. In addition, it can play a role in epithelial-to-mesenchymal transition by repressing the biogenesis of let-7 microRNAs. In malignancies, LIN28B is frequently overexpressed, which is associated with increased tumor aggressiveness and metastatic properties. In this review, we discuss the molecular mechanisms of LIN28B in promoting tumor progression and metastasis in solid tumor entities and its potential use as a clinical therapeutic target and biomarker. More >

TINGTING SUN, XUE SUN, XIN WANG, RUI GUO, YUANHUA YU^*, LE GAO^*

BIOCELL, Vol.47, No.4, pp. 879-889, 2023, DOI:10.32604/biocell.2023.026229

Abstract Background: Aldo-keto oxidoreductase (AKR) inhibitors could reverse the resistance of several cancer cells to cis-platin, but their role in resistance remains unclear.Methods: We verified the difference of AKR1Cs expression by Western blot, RNA sequencing and qRT-PCR. The differences of AKR1Cs expression were analyzed and inferred. Use Assay of NADH and NAD⁺ content to verify the inference. The Docking experience was used to verify the affinity between MPA, MCFLA, MLS and AKR1C3.Results: Our RNA-seq results showed de novo NAD biosynthesis-related genes and NAD(P)H-dependent oxidoreductases were significantly upregulated in cis-platin-resistant HepG2 hepatic cancer cells (HepG2-RC cells) compared with HepG2 cells. At least… More >

Guoqing Long¹, Guogang Zhang¹, Qi Zhang², Cuilin Zhao², Suoying He^2,*, Fengzhong Sun²

FDMP-Fluid Dynamics & Materials Processing, Vol.19, No.7, pp. 1789-1801, 2023, DOI:10.32604/fdmp.2023.026559

Abstract In recent years, water collecting systems, with the associated advantages of energy saving and noise reduction, have become the foundation for the development of a scheme to optimize the structure of cooling towers. To explore the feasibility of this approach for mechanical draft cooling towers, a small-scale experimental device has been built to study the resistance and splash performances of three U-type water collecting devices (WCDs) for different water flow rates and wind speeds. The experimental results show that within the considered ranges of wind speed and water flow rate, the pressure drop of the different WCDs can vary significantly.… More > Graphic Abstract