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  • Open Access


    Identification of Secondary Metabolites in Tunisian Tilia platyphyllos Scop. Using MALDI-TOF and GC-MS

    Ayda Khadhri1, Mohamed Mendili1, Marwa Bannour-Scharinger1, Eric Masson2, Antonio Pizzi2,*

    Journal of Renewable Materials, Vol.12, No.4, pp. 827-842, 2024, DOI:10.32604/jrm.2024.046950

    Abstract This study is the first to evaluate the phytochemical content and biological properties of Tunisian T. platyphyllos Scop. A total of 23 compounds of essential oils were identified by gas chromatography-mass spectrometry (GC-MS) analysis of bracts and fruit extracts. The results show that oxygenated monoterpenes were the dominant class of essential oils. The phenolic composition was investigated by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). The analysis showed that the chemical profiles of the ethanolic extracts of bracts and fruits are substantially similar. The highest polyphenol content was found in the ethanolic extracts of the fruits (7.65… More > Graphic Abstract

    Identification of Secondary Metabolites in Tunisian <i>Tilia platyphyllos</i> Scop. Using MALDI-TOF and GC-MS

  • Open Access


    A perspective review on the biosynthesis of plant-based secondary metabolites and their application as potent drugs


    BIOCELL, Vol.48, No.4, pp. 541-557, 2024, DOI:10.32604/biocell.2023.029031

    Abstract Many phytochemicals and their derived metabolites produced by plants are extensively employed in commercial goods, pharmaceutical products as well as in the environmental and medical fields. However, these secondary metabolites obtained from plants are in low amounts, and it is difficult to synthesize them at the industrial level. Despite these challenges, they may be utilized for a variety of medicinal products that are either available in the market or are being researched and tested. Secondary metabolites are complex compounds that exhibit chirality. Further, under controlled conditions with elicitors, desired secondary metabolites may be produced from More > Graphic Abstract

    A perspective review on the biosynthesis of plant-based secondary metabolites and their application as potent drugs

  • Open Access


    The Identification of Phenylalanine Ammonia-Lyase (PAL) Genes from Pinus yunnanensis and an Analysis of Enzyme Activity in vitro

    Dejin Mu1,2, Lin Chen1,2, Heze Wang1,2, Zhaoliu Hu1,2, Sihui Chen1,2, Shi Chen1,2, Nianhui Cai1,2, Yulan Xu1,2, Junrong Tang1,2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 503-516, 2024, DOI:10.32604/phyton.2024.048786

    Abstract Phenylalanine ammonia lyase (PAL) is the rate-limiting and pivotal enzyme of the general phenylpropanoid pathway, but few reports have been found on PAL genes in Pinus yunnanensis. In the present study, three PAL genes were cloned and identified from P. yunnanensis seedlings for the first time, namely, PyPAL-1, PyPAL-2, and PyPAL-3. Our results indicated that the open-reading frames of PyPAL genes were 2184, 2157, and 2385 bp. Phylogenetic tree analysis revealed that PyPALs have high homology with other known PAL genes in other plants. In vitro enzymatic analysis showed that all three PyPAL recombinant proteins could catalyze the deamination of L-phenylalanine to form More >

  • Open Access


    Selenium: A Game Changer in Plant Development, Growth, and Stress Tolerance, via the Modulation in Gene Expression and Secondary Metabolite Biosynthesis

    Ali Bandehagh1,*, Zahra Dehghanian2, Vahideh Gougerdchi1, Mohammad Anwar Hossain3,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2301-2324, 2023, DOI:10.32604/phyton.2023.028586

    Abstract The presence of selenium (Se) is not widely established as crucial for crops, although it is commonly recognized as an important nutrient for animals as well as humans. Even so, it is inevitably accepted that Se usually contributes positively to the life cycle of plants. Previous findings suggested that small amounts of Se seem to have a productive role in growth and production. As a result, Se is assumed to function in multiple ways, primarily by influencing a variety of biochemical and physiological functions. Also, Se also acts as a plant antioxidant and pro-oxidant and… More >

  • Open Access


    Integrated Data Reveal the Flavonoid Biosynthesis Metabolic Pathway in Semen Ziziphi Spinosae Using Transcriptomic and Metabolomic

    Wei Tian1, Lingdi Liu1, Zhongxing Song2, Zhishu Tang2, Chunxiu Wen1, Tao Jiang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 369-382, 2023, DOI:10.32604/phyton.2022.023184

    Abstract The extract from Semen Ziziphi Spinosae (SZS) contains many secondary metabolites (flavonoids, alkaloids, and terpenoids), widely reported for their high medicinal value. In order to know the number and type of flavonoids and the regulating mechanism of flavonoid biosynthesis during the growth and development of SZS, we identified and analyzed the flavonoid metabolites and genes in SZS using metabolomic and transcriptomic. A total of 13,232 differentially expressed genes and 83 flavonoid metabolites were identified at three different growth and development stages (T1, T2, and T3) of SZS. The main flavonoid metabolic components of SZS were catechin, More >

  • Open Access


    Impact of nanomaterials on plants: What other implications do they have?


    BIOCELL, Vol.46, No.3, pp. 651-654, 2022, DOI:10.32604/biocell.2022.017350

    Abstract The use of nanomaterials has spread and has been applied in different industries, including agriculture. Here, the possibilities presented by NMs are very varied, from the biostimulation of favorable responses, or the control of pests and diseases, to the monitoring of characteristics of interest with the use of nanosensors. Particularly the biostimulation of agricultural crops with the use of nanomaterials is very relevant, since from this process stress tolerance, higher content of biocompounds, etc., can be induced. Although the positive impacts on crops are clear, there is not enough information to determine the long-term impacts, More >

  • Open Access


    Isolation, purification and structure elucidation of three new bioactive secondary metabolites from Streptomyces lividans AM


    BIOCELL, Vol.45, No.5, pp. 1263-1271, 2021, DOI:10.32604/biocell.2021.013198

    Abstract Microorganisms are a huge mine of bioactive metabolites, and actinomycetes are one of the very active groups in this area. In this article, we are concerned about the full taxonomical characterization of Streptomyces lividans AM, isolated from Egyptian soil. This isolate produced three new bioactive metabolites, namely: 1-Nona-decanoyl,4-oleyl disuccinate (1), filoboletic acid; (9Z,11E)-8,13-dihydroxy octadeca-9,11-dienoic acid (2), and sitosteryl-3β-D-glucoside (3). Extensive 1D and 2D NMR and HR-mass spectrometry were used to elucidate the structures of the three compounds. Moreover, ten known compounds were also identified. The antimicrobial activity of the producing organism and newly reported compounds (1–3) More >

  • Open Access


    Genome-wide identification of WRKY gene family and expression analysis under abiotic stresses in Andrographis paniculata


    BIOCELL, Vol.45, No.4, pp. 1107-1119, 2021, DOI:10.32604/biocell.2021.015282

    Abstract Andrographis paniculata (A. paniculata) is a Chinese herbal medicine that clears away heat, reduces inflammation, protects the liver, and promotes choleretics. The WRKYs of A. paniculata are still not well characterized, although many WRKYs have been identified in various plant species. In the present study, 59 A. paniculata WRKY (ApWRKY) genes were identified and renamed on the basis of their respective chromosome distribution. These ApWRKYs were divided into three groups via phylogenetic analysis according to their WRKY domains and combined with WRKY of Arabidopsis. The 59 identified ApWRKY transcription factors were nonuniformity distributed on 23 chromosomes of A. paniculata. From the structural… More >

  • Open Access


    In vitro Antibacterial Activity of Moringa oleifera Ethanolic Extract against Tomato Phytopathogenic Bacteria

    Roberto Arredondo-Valdés1, Francisco D. Hernández-Castillo2, Mario Rocandio-Rodríguez1, Julia C. Anguiano-Cabello3, Madai Rosas-Mejía1, Venancio Vanoye-Eligio1, Salvador Ordaz-Silva4, Imelda V. López-Sánchez4, Laura D. Carrazco-Peña4, Julio C. Chacón-Hernández1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 895-906, 2021, DOI:10.32604/phyton.2021.014301

    Abstract The tomato (Solanum lycopersicum L.) is one of the world’s most important vegetable crops. Still, phytopathogenic bacteria affect the yield and quality of tomato cultivation, like Agrobacterium tumefeciens (At), Clavibacter michiganensis subsp. michiganensis (Cmm), Pseudomonas syringae pv. tomato (Pst), Ralstonia solanacearum (Rs), and Xanthomonas axonopodis (Xa). Synthetic chemical products are used mostly on disease plant control, but overuse generates resistance to bacterial control. This study aimed to evaluate the in vitro antibacterial activity of the ethanolic extract of Moringa oleifera Lam. leaves against At, Cmm, Pst, Rs, and Xa, as well as information about this plant species’ chemical composition. Antibacterial activity against pathogens observed by microplate technique, phytochemical screening, More >

  • Open Access


    Evaluation of Ocimum basilicum L. with Different Concentrations of K+ as an Inhibitor of Pathogenic Bacterial Strains

    Mercedes Georgina Ramírez-Aragón1, Victoria Jared Borroel-García1, Ramón Valenzuela-Soto1, Ricardo David Valdez-Cepeda2, Francisco Javier Wong-Corral3, José Luis García-Hernández4,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 533-541, 2021, DOI:10.32604/phyton.2021.013166

    Abstract The extraction of bioactive compounds has become one of the most interesting areas of modern chemistry. For therapeutic reasons, it´s important to obtain antimicrobial agents from natural origin. The objective of the present study was to determine the inhibitory effect of ethanolic extract of basil (Ocimum basilicum L. var. Red Rubin) subjected to different concentrations of potassium (K+) on the activity of three bacterial strains that are pathogens in humans. Susceptibility was evaluated by inhibition surface and these results were compared to two antibiotics: Gentamicin (GE) and Ciprofloxacin (CPF) for their efficacy against each bacterial strain. Analyzed More >

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